Vibrio viridaestus
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4277 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N9TIE4|A0A3N9TIE4_9VIBR HlyD family efflux transporter periplasmic adaptor subunit OS=Vibrio viridaestus OX=2487322 GN=EES38_10545 PE=3 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.41 KK3 pKa = 10.03 LLPVATGAFALLTLSGFAQAADD25 pKa = 3.78 NDD27 pKa = 3.74 YY28 pKa = 11.31 GLYY31 pKa = 10.55 VGGNYY36 pKa = 10.46 GYY38 pKa = 11.25 VKK40 pKa = 10.7 VDD42 pKa = 3.45 GQDD45 pKa = 5.18 DD46 pKa = 4.0 FDD48 pKa = 6.95 DD49 pKa = 5.91 DD50 pKa = 4.29 NDD52 pKa = 3.87 VYY54 pKa = 11.12 QGLLGFRR61 pKa = 11.84 FNKK64 pKa = 9.99 YY65 pKa = 9.43 LALEE69 pKa = 4.28 GSYY72 pKa = 10.46 IDD74 pKa = 3.94 FGDD77 pKa = 3.72 YY78 pKa = 10.77 GKK80 pKa = 10.77 SGASASTDD88 pKa = 3.1 GYY90 pKa = 8.95 TAALKK95 pKa = 10.54 VIAPIGEE102 pKa = 4.03 RR103 pKa = 11.84 VEE105 pKa = 5.08 LYY107 pKa = 10.99 GKK109 pKa = 10.43 GGQLWYY115 pKa = 8.93 NTDD118 pKa = 3.42 YY119 pKa = 11.51 SVLGVNGSRR128 pKa = 11.84 DD129 pKa = 3.75 DD130 pKa = 3.81 EE131 pKa = 4.7 GVFAGAGVGFKK142 pKa = 9.59 VTDD145 pKa = 3.42 NFLVNVEE152 pKa = 4.26 YY153 pKa = 9.3 TWYY156 pKa = 10.38 DD157 pKa = 2.99 ADD159 pKa = 3.59 IDD161 pKa = 4.06 ADD163 pKa = 3.94 AVSNGADD170 pKa = 3.28 TEE172 pKa = 4.46 TDD174 pKa = 3.93 FKK176 pKa = 10.67 QASVGVEE183 pKa = 3.67 YY184 pKa = 10.87 RR185 pKa = 11.84 FF186 pKa = 3.62
Molecular weight: 20.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.897
IPC_protein 3.91
Toseland 3.668
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.579
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.317
Thurlkill 3.732
EMBOSS 3.872
Sillero 4.024
Patrickios 1.138
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.913
Protein with the highest isoelectric point:
>tr|A0A3N9TBA4|A0A3N9TBA4_9VIBR Uncharacterized protein OS=Vibrio viridaestus OX=2487322 GN=EES38_21535 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 7.65 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 KK29 pKa = 9.39 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSKK44 pKa = 10.83
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4277
0
4277
1367418
19
2360
319.7
35.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.98 ± 0.037
1.096 ± 0.013
5.525 ± 0.027
6.218 ± 0.035
4.217 ± 0.026
6.739 ± 0.035
2.242 ± 0.018
7.007 ± 0.033
5.582 ± 0.03
10.273 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.642 ± 0.017
4.316 ± 0.023
3.816 ± 0.022
4.195 ± 0.028
4.429 ± 0.025
7.038 ± 0.031
5.314 ± 0.025
6.967 ± 0.029
1.239 ± 0.013
3.142 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here