Streptomyces sp. Ag82_G5-5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9095 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N4T6K0|A0A3N4T6K0_9ACTN Mechanosensitive ion channel-like protein OS=Streptomyces sp. Ag82_G5-5 OX=2485160 GN=EDE03_8721 PE=4 SV=1
MM1 pKa = 6.5QQEE4 pKa = 4.22AGSGGEE10 pKa = 4.19ALEE13 pKa = 4.03VWIDD17 pKa = 3.41QDD19 pKa = 3.99LCTGDD24 pKa = 4.65GICVQYY30 pKa = 10.96APEE33 pKa = 4.18VFEE36 pKa = 5.9LDD38 pKa = 3.05IDD40 pKa = 3.63GLAYY44 pKa = 10.54VRR46 pKa = 11.84GAGEE50 pKa = 4.63EE51 pKa = 4.06LLQAPGATTPVPLPLLTDD69 pKa = 3.6VVDD72 pKa = 4.12SAKK75 pKa = 10.15EE76 pKa = 3.93CPGEE80 pKa = 4.38CIHH83 pKa = 6.24VRR85 pKa = 11.84RR86 pKa = 11.84VSDD89 pKa = 3.32RR90 pKa = 11.84VEE92 pKa = 4.08VFGPDD97 pKa = 3.07AEE99 pKa = 4.33

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N4VJD2|A0A3N4VJD2_9ACTN Uncharacterized protein OS=Streptomyces sp. Ag82_G5-5 OX=2485160 GN=EDE03_0160 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9095

0

9095

3100265

29

9508

340.9

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.258 ± 0.039

0.748 ± 0.007

6.087 ± 0.023

5.563 ± 0.029

2.795 ± 0.015

9.387 ± 0.028

2.249 ± 0.013

3.229 ± 0.022

2.241 ± 0.026

10.324 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.683 ± 0.01

1.88 ± 0.017

5.976 ± 0.024

2.817 ± 0.015

7.73 ± 0.034

5.308 ± 0.022

6.432 ± 0.024

8.557 ± 0.024

1.555 ± 0.011

2.179 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski