Amino acid dipepetide frequency for Streptomyces sp. Ag82_G5-5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.995AlaAla: 19.995 ± 0.114	0.996AlaCys: 0.996 ± 0.02	8.428AlaAsp: 8.428 ± 0.063	8.437AlaGlu: 8.437 ± 0.076	3.518AlaPhe: 3.518 ± 0.037	12.628AlaGly: 12.628 ± 0.07	2.769AlaHis: 2.769 ± 0.03	3.567AlaIle: 3.567 ± 0.045	3.021AlaLys: 3.021 ± 0.045	14.28AlaLeu: 14.28 ± 0.101	2.339AlaMet: 2.339 ± 0.032	2.06AlaAsn: 2.06 ± 0.033	6.657AlaPro: 6.657 ± 0.059	3.86AlaGln: 3.86 ± 0.037	9.812AlaArg: 9.812 ± 0.062	6.163AlaSer: 6.163 ± 0.054	7.311AlaThr: 7.311 ± 0.058	12.034AlaVal: 12.034 ± 0.076	1.88AlaTrp: 1.88 ± 0.026	2.828AlaTyr: 2.828 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.032CysAla: 1.032 ± 0.021	0.092CysCys: 0.092 ± 0.006	0.453CysAsp: 0.453 ± 0.014	0.379CysGlu: 0.379 ± 0.009	0.196CysPhe: 0.196 ± 0.009	0.909CysGly: 0.909 ± 0.021	0.178CysHis: 0.178 ± 0.008	0.154CysIle: 0.154 ± 0.008	0.113CysLys: 0.113 ± 0.006	0.734CysLeu: 0.734 ± 0.017	0.121CysMet: 0.121 ± 0.007	0.135CysAsn: 0.135 ± 0.007	0.451CysPro: 0.451 ± 0.012	0.162CysGln: 0.162 ± 0.007	0.533CysArg: 0.533 ± 0.015	0.415CysSer: 0.415 ± 0.013	0.497CysThr: 0.497 ± 0.014	0.647CysVal: 0.647 ± 0.014	0.12CysTrp: 0.12 ± 0.007	0.155CysTyr: 0.155 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.495AspAla: 7.495 ± 0.063	0.421AspCys: 0.421 ± 0.012	3.646AspAsp: 3.646 ± 0.039	3.882AspGlu: 3.882 ± 0.046	1.76AspPhe: 1.76 ± 0.026	6.51AspGly: 6.51 ± 0.06	1.426AspHis: 1.426 ± 0.023	1.993AspIle: 1.993 ± 0.024	1.256AspLys: 1.256 ± 0.024	6.313AspLeu: 6.313 ± 0.052	0.845AspMet: 0.845 ± 0.019	1.07AspAsn: 1.07 ± 0.023	4.525AspPro: 4.525 ± 0.041	1.58AspGln: 1.58 ± 0.025	4.811AspArg: 4.811 ± 0.044	2.779AspSer: 2.779 ± 0.033	3.386AspThr: 3.386 ± 0.037	4.906AspVal: 4.906 ± 0.044	1.072AspTrp: 1.072 ± 0.021	1.191AspTyr: 1.191 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.047GluAla: 7.047 ± 0.065	0.348GluCys: 0.348 ± 0.012	2.736GluAsp: 2.736 ± 0.034	3.418GluGlu: 3.418 ± 0.041	1.544GluPhe: 1.544 ± 0.024	4.268GluGly: 4.268 ± 0.045	1.463GluHis: 1.463 ± 0.022	2.19GluIle: 2.19 ± 0.031	1.397GluLys: 1.397 ± 0.025	6.814GluLeu: 6.814 ± 0.064	0.837GluMet: 0.837 ± 0.019	1.03GluAsn: 1.03 ± 0.017	3.283GluPro: 3.283 ± 0.041	2.202GluGln: 2.202 ± 0.029	5.203GluArg: 5.203 ± 0.051	2.643GluSer: 2.643 ± 0.036	2.937GluThr: 2.937 ± 0.029	4.373GluVal: 4.373 ± 0.045	0.77GluTrp: 0.77 ± 0.018	1.131GluTyr: 1.131 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	3.694PheAla: 3.694 ± 0.038	0.253PheCys: 0.253 ± 0.01	2.003PheAsp: 2.003 ± 0.027	1.435PheGlu: 1.435 ± 0.023	0.883PhePhe: 0.883 ± 0.021	3.146PheGly: 3.146 ± 0.037	0.623PheHis: 0.623 ± 0.015	0.735PheIle: 0.735 ± 0.018	0.542PheLys: 0.542 ± 0.014	2.653PheLeu: 2.653 ± 0.037	0.414PheMet: 0.414 ± 0.013	0.591PheAsn: 0.591 ± 0.015	1.43PhePro: 1.43 ± 0.023	0.734PheGln: 0.734 ± 0.017	1.823PheArg: 1.823 ± 0.028	1.5PheSer: 1.5 ± 0.026	2.104PheThr: 2.104 ± 0.027	2.344PheVal: 2.344 ± 0.028	0.435PheTrp: 0.435 ± 0.011	0.607PheTyr: 0.607 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	10.866GlyAla: 10.866 ± 0.069	0.795GlyCys: 0.795 ± 0.017	5.232GlyAsp: 5.232 ± 0.053	4.981GlyGlu: 4.981 ± 0.049	2.934GlyPhe: 2.934 ± 0.033	8.582GlyGly: 8.582 ± 0.076	2.218GlyHis: 2.218 ± 0.031	3.565GlyIle: 3.565 ± 0.041	2.479GlyLys: 2.479 ± 0.034	9.413GlyLeu: 9.413 ± 0.057	1.913GlyMet: 1.913 ± 0.026	1.879GlyAsn: 1.879 ± 0.029	4.963GlyPro: 4.963 ± 0.053	2.714GlyGln: 2.714 ± 0.03	7.317GlyArg: 7.317 ± 0.061	5.632GlySer: 5.632 ± 0.047	6.672GlyThr: 6.672 ± 0.061	7.621GlyVal: 7.621 ± 0.058	1.735GlyTrp: 1.735 ± 0.028	2.362GlyTyr: 2.362 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.581HisAla: 2.581 ± 0.033	0.199HisCys: 0.199 ± 0.007	1.368HisAsp: 1.368 ± 0.021	1.215HisGlu: 1.215 ± 0.021	0.669HisPhe: 0.669 ± 0.015	2.316HisGly: 2.316 ± 0.028	0.676HisHis: 0.676 ± 0.016	0.705HisIle: 0.705 ± 0.014	0.359HisLys: 0.359 ± 0.011	2.347HisLeu: 2.347 ± 0.028	0.318HisMet: 0.318 ± 0.01	0.391HisAsn: 0.391 ± 0.011	1.78HisPro: 1.78 ± 0.027	0.636HisGln: 0.636 ± 0.014	2.019HisArg: 2.019 ± 0.027	0.999HisSer: 0.999 ± 0.019	1.359HisThr: 1.359 ± 0.023	1.693HisVal: 1.693 ± 0.025	0.373HisTrp: 0.373 ± 0.01	0.488HisTyr: 0.488 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.851IleAla: 4.851 ± 0.045	0.287IleCys: 0.287 ± 0.009	2.296IleAsp: 2.296 ± 0.029	1.958IleGlu: 1.958 ± 0.028	0.703IlePhe: 0.703 ± 0.017	3.631IleGly: 3.631 ± 0.04	0.627IleHis: 0.627 ± 0.014	0.873IleIle: 0.873 ± 0.02	0.775IleLys: 0.775 ± 0.018	2.448IleLeu: 2.448 ± 0.036	0.441IleMet: 0.441 ± 0.013	0.718IleAsn: 0.718 ± 0.015	1.855IlePro: 1.855 ± 0.029	0.775IleGln: 0.775 ± 0.016	2.253IleArg: 2.253 ± 0.031	1.733IleSer: 1.733 ± 0.026	2.335IleThr: 2.335 ± 0.031	2.783IleVal: 2.783 ± 0.039	0.382IleTrp: 0.382 ± 0.012	0.561IleTyr: 0.561 ± 0.013	0.0IleXaa: 0.0 ± 0.0
Lys	3.06LysAla: 3.06 ± 0.04	0.121LysCys: 0.121 ± 0.008	1.441LysAsp: 1.441 ± 0.029	1.182LysGlu: 1.182 ± 0.022	0.474LysPhe: 0.474 ± 0.013	1.936LysGly: 1.936 ± 0.029	0.424LysHis: 0.424 ± 0.011	0.859LysIle: 0.859 ± 0.019	0.907LysLys: 0.907 ± 0.028	2.05LysLeu: 2.05 ± 0.032	0.381LysMet: 0.381 ± 0.012	0.557LysAsn: 0.557 ± 0.013	1.359LysPro: 1.359 ± 0.024	0.747LysGln: 0.747 ± 0.018	1.386LysArg: 1.386 ± 0.025	1.279LysSer: 1.279 ± 0.025	1.38LysThr: 1.38 ± 0.025	2.039LysVal: 2.039 ± 0.031	0.314LysTrp: 0.314 ± 0.01	0.514LysTyr: 0.514 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.919LeuAla: 14.919 ± 0.082	0.8LeuCys: 0.8 ± 0.017	6.822LeuAsp: 6.822 ± 0.05	4.469LeuGlu: 4.469 ± 0.049	2.658LeuPhe: 2.658 ± 0.034	9.202LeuGly: 9.202 ± 0.066	2.276LeuHis: 2.276 ± 0.031	3.327LeuIle: 3.327 ± 0.041	2.142LeuLys: 2.142 ± 0.032	11.256LeuLeu: 11.256 ± 0.097	1.58LeuMet: 1.58 ± 0.022	1.808LeuAsn: 1.808 ± 0.028	6.413LeuPro: 6.413 ± 0.049	2.212LeuGln: 2.212 ± 0.03	8.458LeuArg: 8.458 ± 0.066	5.481LeuSer: 5.481 ± 0.043	7.227LeuThr: 7.227 ± 0.051	8.927LeuVal: 8.927 ± 0.069	1.339LeuTrp: 1.339 ± 0.024	1.924LeuTyr: 1.924 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.109MetAla: 2.109 ± 0.031	0.119MetCys: 0.119 ± 0.007	0.865MetAsp: 0.865 ± 0.018	0.695MetGlu: 0.695 ± 0.018	0.451MetPhe: 0.451 ± 0.013	1.296MetGly: 1.296 ± 0.022	0.339MetHis: 0.339 ± 0.01	0.649MetIle: 0.649 ± 0.015	0.404MetLys: 0.404 ± 0.012	1.637MetLeu: 1.637 ± 0.02	0.294MetMet: 0.294 ± 0.011	0.438MetAsn: 0.438 ± 0.012	1.075MetPro: 1.075 ± 0.016	0.43MetGln: 0.43 ± 0.012	1.36MetArg: 1.36 ± 0.02	1.33MetSer: 1.33 ± 0.02	1.571MetThr: 1.571 ± 0.023	1.234MetVal: 1.234 ± 0.019	0.214MetTrp: 0.214 ± 0.009	0.319MetTyr: 0.319 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.279AsnAla: 2.279 ± 0.025	0.178AsnCys: 0.178 ± 0.008	1.022AsnAsp: 1.022 ± 0.021	0.838AsnGlu: 0.838 ± 0.017	0.515AsnPhe: 0.515 ± 0.015	2.043AsnGly: 2.043 ± 0.032	0.408AsnHis: 0.408 ± 0.011	0.693AsnIle: 0.693 ± 0.017	0.435AsnLys: 0.435 ± 0.014	1.723AsnLeu: 1.723 ± 0.025	0.307AsnMet: 0.307 ± 0.01	0.492AsnAsn: 0.492 ± 0.018	1.409AsnPro: 1.409 ± 0.022	0.545AsnGln: 0.545 ± 0.013	1.321AsnArg: 1.321 ± 0.022	1.13AsnSer: 1.13 ± 0.023	1.227AsnThr: 1.227 ± 0.024	1.451AsnVal: 1.451 ± 0.024	0.333AsnTrp: 0.333 ± 0.012	0.451AsnTyr: 0.451 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	8.247ProAla: 8.247 ± 0.068	0.307ProCys: 0.307 ± 0.011	4.504ProAsp: 4.504 ± 0.044	4.187ProGlu: 4.187 ± 0.044	1.545ProPhe: 1.545 ± 0.023	6.218ProGly: 6.218 ± 0.053	1.356ProHis: 1.356 ± 0.02	1.346ProIle: 1.346 ± 0.022	1.274ProLys: 1.274 ± 0.024	5.299ProLeu: 5.299 ± 0.05	0.966ProMet: 0.966 ± 0.018	0.981ProAsn: 0.981 ± 0.019	3.4ProPro: 3.4 ± 0.048	1.785ProGln: 1.785 ± 0.032	3.704ProArg: 3.704 ± 0.041	3.36ProSer: 3.36 ± 0.037	3.558ProThr: 3.558 ± 0.038	5.399ProVal: 5.399 ± 0.039	0.895ProTrp: 0.895 ± 0.02	1.435ProTyr: 1.435 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.605GlnAla: 3.605 ± 0.046	0.167GlnCys: 0.167 ± 0.007	1.475GlnAsp: 1.475 ± 0.024	1.476GlnGlu: 1.476 ± 0.023	0.728GlnPhe: 0.728 ± 0.014	2.38GlnGly: 2.38 ± 0.027	0.665GlnHis: 0.665 ± 0.016	1.095GlnIle: 1.095 ± 0.021	0.647GlnLys: 0.647 ± 0.015	3.187GlnLeu: 3.187 ± 0.031	0.479GlnMet: 0.479 ± 0.014	0.538GlnAsn: 0.538 ± 0.015	1.723GlnPro: 1.723 ± 0.03	1.262GlnGln: 1.262 ± 0.024	2.322GlnArg: 2.322 ± 0.034	1.359GlnSer: 1.359 ± 0.022	1.422GlnThr: 1.422 ± 0.024	2.473GlnVal: 2.473 ± 0.034	0.506GlnTrp: 0.506 ± 0.015	0.658GlnTyr: 0.658 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.439ArgAla: 9.439 ± 0.071	0.534ArgCys: 0.534 ± 0.014	4.127ArgAsp: 4.127 ± 0.041	4.592ArgGlu: 4.592 ± 0.048	2.301ArgPhe: 2.301 ± 0.026	5.526ArgGly: 5.526 ± 0.051	2.012ArgHis: 2.012 ± 0.028	3.199ArgIle: 3.199 ± 0.034	1.572ArgLys: 1.572 ± 0.027	8.546ArgLeu: 8.546 ± 0.074	1.635ArgMet: 1.635 ± 0.024	1.324ArgAsn: 1.324 ± 0.02	4.708ArgPro: 4.708 ± 0.049	2.266ArgGln: 2.266 ± 0.027	7.243ArgArg: 7.243 ± 0.07	3.988ArgSer: 3.988 ± 0.039	5.432ArgThr: 5.432 ± 0.041	5.775ArgVal: 5.775 ± 0.048	1.317ArgTrp: 1.317 ± 0.02	1.765ArgTyr: 1.765 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	7.076SerAla: 7.076 ± 0.05	0.405SerCys: 0.405 ± 0.014	2.938SerAsp: 2.938 ± 0.038	2.412SerGlu: 2.412 ± 0.028	1.598SerPhe: 1.598 ± 0.024	6.166SerGly: 6.166 ± 0.053	1.017SerHis: 1.017 ± 0.017	1.529SerIle: 1.529 ± 0.026	1.117SerLys: 1.117 ± 0.021	5.024SerLeu: 5.024 ± 0.049	1.102SerMet: 1.102 ± 0.019	0.983SerAsn: 0.983 ± 0.02	3.229SerPro: 3.229 ± 0.036	1.327SerGln: 1.327 ± 0.021	3.619SerArg: 3.619 ± 0.036	3.181SerSer: 3.181 ± 0.046	3.48SerThr: 3.48 ± 0.038	4.563SerVal: 4.563 ± 0.043	0.955SerTrp: 0.955 ± 0.019	1.361SerTyr: 1.361 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	9.192ThrAla: 9.192 ± 0.062	0.412ThrCys: 0.412 ± 0.013	3.9ThrAsp: 3.9 ± 0.039	3.352ThrGlu: 3.352 ± 0.035	1.741ThrPhe: 1.741 ± 0.028	6.923ThrGly: 6.923 ± 0.056	1.266ThrHis: 1.266 ± 0.019	1.822ThrIle: 1.822 ± 0.028	1.341ThrLys: 1.341 ± 0.022	5.979ThrLeu: 5.979 ± 0.052	0.911ThrMet: 0.911 ± 0.018	1.151ThrAsn: 1.151 ± 0.021	4.402ThrPro: 4.402 ± 0.042	1.43ThrGln: 1.43 ± 0.023	3.953ThrArg: 3.953 ± 0.036	3.554ThrSer: 3.554 ± 0.04	4.18ThrThr: 4.18 ± 0.059	6.343ThrVal: 6.343 ± 0.047	0.962ThrTrp: 0.962 ± 0.018	1.512ThrTyr: 1.512 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.559ValAla: 10.559 ± 0.072	0.724ValCys: 0.724 ± 0.019	5.156ValAsp: 5.156 ± 0.042	4.699ValGlu: 4.699 ± 0.05	2.506ValPhe: 2.506 ± 0.031	6.722ValGly: 6.722 ± 0.049	1.972ValHis: 1.972 ± 0.022	2.88ValIle: 2.88 ± 0.037	1.809ValLys: 1.809 ± 0.027	9.568ValLeu: 9.568 ± 0.07	1.388ValMet: 1.388 ± 0.02	1.749ValAsn: 1.749 ± 0.027	5.217ValPro: 5.217 ± 0.046	2.197ValGln: 2.197 ± 0.028	7.076ValArg: 7.076 ± 0.055	4.513ValSer: 4.513 ± 0.037	5.829ValThr: 5.829 ± 0.055	8.181ValVal: 8.181 ± 0.068	1.2ValTrp: 1.2 ± 0.021	1.63ValTyr: 1.63 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.669TrpAla: 1.669 ± 0.031	0.139TrpCys: 0.139 ± 0.008	0.834TrpAsp: 0.834 ± 0.018	0.708TrpGlu: 0.708 ± 0.015	0.531TrpPhe: 0.531 ± 0.015	1.095TrpGly: 1.095 ± 0.018	0.391TrpHis: 0.391 ± 0.011	0.575TrpIle: 0.575 ± 0.013	0.379TrpLys: 0.379 ± 0.012	1.819TrpLeu: 1.819 ± 0.028	0.289TrpMet: 0.289 ± 0.009	0.442TrpAsn: 0.442 ± 0.014	0.803TrpPro: 0.803 ± 0.017	0.663TrpGln: 0.663 ± 0.017	1.325TrpArg: 1.325 ± 0.022	1.021TrpSer: 1.021 ± 0.022	1.111TrpThr: 1.111 ± 0.024	1.012TrpVal: 1.012 ± 0.019	0.364TrpTrp: 0.364 ± 0.012	0.386TrpTyr: 0.386 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.867TyrAla: 2.867 ± 0.035	0.178TyrCys: 0.178 ± 0.008	1.622TyrAsp: 1.622 ± 0.028	1.317TyrGlu: 1.317 ± 0.021	0.695TyrPhe: 0.695 ± 0.018	2.376TyrGly: 2.376 ± 0.03	0.386TyrHis: 0.386 ± 0.012	0.528TyrIle: 0.528 ± 0.013	0.44TyrLys: 0.44 ± 0.014	2.151TyrLeu: 2.151 ± 0.03	0.273TyrMet: 0.273 ± 0.01	0.466TyrAsn: 0.466 ± 0.015	1.082TyrPro: 1.082 ± 0.018	0.64TyrGln: 0.64 ± 0.016	1.763TyrArg: 1.763 ± 0.028	1.022TyrSer: 1.022 ± 0.022	1.308TyrThr: 1.308 ± 0.02	1.776TyrVal: 1.776 ± 0.024	0.388TyrTrp: 0.388 ± 0.013	0.511TyrTyr: 0.511 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9095 proteins (3100265 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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