Perca fluviatilis (European perch)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamat

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24123 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A5EAC8|A0A6A5EAC8_PERFL Uncharacterized protein OS=Perca fluviatilis OX=8168 GN=PFLUV_G00225870 PE=4 SV=1
MM1 pKa = 7.68DD2 pKa = 4.33HH3 pKa = 7.14CEE5 pKa = 3.97EE6 pKa = 4.31LTAEE10 pKa = 4.02DD11 pKa = 4.95CMWVLCLSPSLSFLQLLTEE30 pKa = 4.5PQQGRR35 pKa = 11.84AAILSVGVACSTLLSDD51 pKa = 4.96PQQQQLALQLLDD63 pKa = 3.73SLVSRR68 pKa = 11.84GQGVLEE74 pKa = 3.99EE75 pKa = 5.72AILQDD80 pKa = 3.65SSSSLDD86 pKa = 2.99LWTSVASLCSEE97 pKa = 4.71LNRR100 pKa = 11.84ADD102 pKa = 3.98LSGG105 pKa = 3.3

Molecular weight: 11.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A5F2M2|A0A6A5F2M2_PERFL C-type lectin domain-containing protein OS=Perca fluviatilis OX=8168 GN=PFLUV_G00126750 PE=4 SV=1
LL1 pKa = 6.23WHH3 pKa = 5.75VTPPPLYY10 pKa = 10.28NPKK13 pKa = 9.9CQHH16 pKa = 5.99CPVYY20 pKa = 10.35RR21 pKa = 11.84PHH23 pKa = 7.77GDD25 pKa = 3.01VRR27 pKa = 11.84TCRR30 pKa = 11.84YY31 pKa = 9.06EE32 pKa = 4.15STQSRR37 pKa = 11.84HH38 pKa = 4.75NTRR41 pKa = 11.84KK42 pKa = 9.89SPMPKK47 pKa = 8.98RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84VAPLNRR56 pKa = 11.84RR57 pKa = 11.84FLGGVCVPRR66 pKa = 11.84EE67 pKa = 4.05SEE69 pKa = 3.96RR70 pKa = 11.84EE71 pKa = 3.68RR72 pKa = 11.84EE73 pKa = 4.0RR74 pKa = 11.84EE75 pKa = 3.79KK76 pKa = 10.53EE77 pKa = 3.78RR78 pKa = 11.84EE79 pKa = 3.94RR80 pKa = 11.84EE81 pKa = 3.89RR82 pKa = 11.84EE83 pKa = 3.79RR84 pKa = 11.84GRR86 pKa = 11.84GRR88 pKa = 11.84ALINNNSAFRR98 pKa = 11.84SRR100 pKa = 11.84FYY102 pKa = 10.46WIQLFTASSVKK113 pKa = 9.92KK114 pKa = 10.2RR115 pKa = 11.84LRR117 pKa = 11.84GLCLCYY123 pKa = 10.17FFPNILSPVVHH134 pKa = 5.97TFIVEE139 pKa = 3.9CHH141 pKa = 6.38CGSEE145 pKa = 3.99KK146 pKa = 10.74LFRR149 pKa = 11.84HH150 pKa = 5.95DD151 pKa = 5.85AEE153 pKa = 5.75LGLCGHH159 pKa = 7.69LAANPEE165 pKa = 4.26KK166 pKa = 10.78APSRR170 pKa = 11.84DD171 pKa = 3.28VRR173 pKa = 11.84SFRR176 pKa = 11.84YY177 pKa = 9.26PRR179 pKa = 11.84AHH181 pKa = 6.89PAQRR185 pKa = 11.84AAPAAGSGWAVV196 pKa = 2.94

Molecular weight: 22.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24123

0

24123

13420900

51

18291

556.4

61.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.665 ± 0.014

2.23 ± 0.013

5.228 ± 0.013

6.924 ± 0.024

3.467 ± 0.011

6.449 ± 0.022

2.629 ± 0.008

4.235 ± 0.013

5.572 ± 0.018

9.433 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.375 ± 0.008

3.801 ± 0.011

5.83 ± 0.021

4.797 ± 0.017

5.69 ± 0.016

8.817 ± 0.018

5.731 ± 0.015

6.319 ± 0.015

1.146 ± 0.005

2.662 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski