Nocardioides alpinus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1A230|A0A1I1A230_9ACTN FMN reductase OS=Nocardioides alpinus OX=748909 GN=SAMN05192575_107144 PE=4 SV=1
MM1 pKa = 7.73PVTDD5 pKa = 3.89VQHH8 pKa = 7.14DD9 pKa = 4.44LDD11 pKa = 5.12ALTLTITADD20 pKa = 4.08FAAPLDD26 pKa = 4.3RR27 pKa = 11.84VWQVYY32 pKa = 10.6ADD34 pKa = 3.68PRR36 pKa = 11.84QLEE39 pKa = 4.61RR40 pKa = 11.84VWGPPGYY47 pKa = 9.25PATFVDD53 pKa = 5.0HH54 pKa = 6.92EE55 pKa = 4.32MAPGGRR61 pKa = 11.84MNYY64 pKa = 9.89FMTSPEE70 pKa = 3.98GEE72 pKa = 3.87NYY74 pKa = 8.28YY75 pKa = 10.65AYY77 pKa = 9.61WVITTVDD84 pKa = 3.56EE85 pKa = 4.65PGGFTFEE92 pKa = 5.61DD93 pKa = 4.47GFALDD98 pKa = 4.01EE99 pKa = 4.4TFAPNPEE106 pKa = 4.2MPVASNDD113 pKa = 3.43YY114 pKa = 11.05AFAEE118 pKa = 4.38ADD120 pKa = 3.13GGTRR124 pKa = 11.84ATFVGTYY131 pKa = 10.16ASAEE135 pKa = 4.02ALQKK139 pKa = 10.46VLDD142 pKa = 3.81MGVVEE147 pKa = 5.41GSTLAINQIDD157 pKa = 4.42DD158 pKa = 4.19LLASS162 pKa = 4.06

Molecular weight: 17.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1AZF2|A0A1I1AZF2_9ACTN Aspartate carbamoyltransferase OS=Nocardioides alpinus OX=748909 GN=pyrB PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4347

0

4347

1417320

29

3754

326.0

34.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.924 ± 0.046

0.698 ± 0.009

6.739 ± 0.033

5.696 ± 0.036

2.815 ± 0.02

9.142 ± 0.04

2.259 ± 0.021

3.536 ± 0.025

1.89 ± 0.029

10.137 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.92 ± 0.016

1.747 ± 0.021

5.516 ± 0.027

2.73 ± 0.017

7.46 ± 0.041

5.496 ± 0.03

6.344 ± 0.045

9.458 ± 0.04

1.604 ± 0.015

1.886 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski