Grapevine virus G

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4N967|A0A2H4N967_9VIRU Movement protein OS=Grapevine virus G OX=2022475 PE=4 SV=1
MM1 pKa = 7.3EE2 pKa = 4.65RR3 pKa = 11.84VEE5 pKa = 4.29NKK7 pKa = 9.42VFKK10 pKa = 10.79VRR12 pKa = 11.84NLKK15 pKa = 10.62NGLSNLQDD23 pKa = 3.33FEE25 pKa = 5.61KK26 pKa = 10.64IVDD29 pKa = 3.57KK30 pKa = 11.24KK31 pKa = 10.76KK32 pKa = 10.22VYY34 pKa = 10.14EE35 pKa = 4.3LGVLDD40 pKa = 5.65EE41 pKa = 4.48IFGPKK46 pKa = 9.32RR47 pKa = 11.84VFKK50 pKa = 10.79CAICKK55 pKa = 9.79EE56 pKa = 4.18LTVEE60 pKa = 4.01SGEE63 pKa = 4.52VNVQMDD69 pKa = 4.56LFCEE73 pKa = 4.09EE74 pKa = 4.42TMDD77 pKa = 5.84GIDD80 pKa = 3.58PDD82 pKa = 4.53VYY84 pKa = 10.66PFMHH88 pKa = 6.82FGCLALGFMGLGSRR102 pKa = 11.84LDD104 pKa = 3.76GQYY107 pKa = 10.28VAQVVDD113 pKa = 3.67TRR115 pKa = 11.84RR116 pKa = 11.84KK117 pKa = 8.71EE118 pKa = 3.99GSNVLSSFRR127 pKa = 11.84FRR129 pKa = 11.84CKK131 pKa = 10.52DD132 pKa = 3.16GVSAYY137 pKa = 9.77IDD139 pKa = 3.81FPDD142 pKa = 3.85FCVATTDD149 pKa = 2.94IMGGFTISIRR159 pKa = 11.84LRR161 pKa = 11.84SEE163 pKa = 5.05GIDD166 pKa = 3.66FMDD169 pKa = 5.38GSHH172 pKa = 7.3PLALNVVALCRR183 pKa = 11.84FMDD186 pKa = 4.73DD187 pKa = 3.38KK188 pKa = 11.79LEE190 pKa = 4.06TKK192 pKa = 9.92MLIKK196 pKa = 10.53KK197 pKa = 9.19HH198 pKa = 5.39GKK200 pKa = 9.74RR201 pKa = 11.84IYY203 pKa = 9.36QAVGQVEE210 pKa = 4.16ILDD213 pKa = 3.95PEE215 pKa = 4.39IGNLRR220 pKa = 11.84LNDD223 pKa = 3.97TPSGDD228 pKa = 4.21VIDD231 pKa = 3.77KK232 pKa = 10.79TMFDD236 pKa = 3.2VAEE239 pKa = 4.98IIRR242 pKa = 11.84KK243 pKa = 8.65IRR245 pKa = 11.84SSVGSGASASTKK257 pKa = 10.0DD258 pKa = 3.29GRR260 pKa = 11.84GIKK263 pKa = 9.74EE264 pKa = 4.57DD265 pKa = 3.76EE266 pKa = 4.54ASSTDD271 pKa = 3.18AAQRR275 pKa = 11.84EE276 pKa = 4.36ASGSKK281 pKa = 9.87VRR283 pKa = 11.84ALFF286 pKa = 3.77

Molecular weight: 31.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4N957|A0A2H4N957_9VIRU Uncharacterized protein OS=Grapevine virus G OX=2022475 PE=4 SV=1
MM1 pKa = 7.05QRR3 pKa = 11.84LGEE6 pKa = 4.35SKK8 pKa = 10.38SAAKK12 pKa = 10.02RR13 pKa = 11.84RR14 pKa = 11.84AKK16 pKa = 10.38RR17 pKa = 11.84YY18 pKa = 7.65GVCYY22 pKa = 10.06KK23 pKa = 10.13CAKK26 pKa = 9.27SVCVCEE32 pKa = 4.36KK33 pKa = 10.64NRR35 pKa = 11.84ASSFDD40 pKa = 3.47RR41 pKa = 11.84TDD43 pKa = 3.1WKK45 pKa = 10.74CYY47 pKa = 8.86NAIRR51 pKa = 11.84MPATRR56 pKa = 11.84YY57 pKa = 9.79LIEE60 pKa = 4.12SRR62 pKa = 11.84GTYY65 pKa = 8.19LHH67 pKa = 6.88KK68 pKa = 10.72ASQLALSDD76 pKa = 4.05LEE78 pKa = 4.73AIGVLGYY85 pKa = 10.45EE86 pKa = 4.24KK87 pKa = 10.42YY88 pKa = 10.65VRR90 pKa = 11.84YY91 pKa = 10.12NKK93 pKa = 8.57STRR96 pKa = 11.84GCKK99 pKa = 9.95SPEE102 pKa = 3.87EE103 pKa = 4.19TPEE106 pKa = 3.49ITISINKK113 pKa = 9.61EE114 pKa = 4.07GTCLAFPLSVV124 pKa = 3.14

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2469

124

1703

493.8

55.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.197 ± 0.74

2.066 ± 0.411

5.67 ± 0.941

7.25 ± 0.496

4.901 ± 0.699

6.602 ± 0.517

2.754 ± 0.516

6.035 ± 0.179

6.926 ± 0.556

10.531 ± 0.987

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.397

4.01 ± 0.483

3.767 ± 0.341

2.673 ± 0.164

5.306 ± 0.53

7.898 ± 0.702

4.253 ± 0.289

6.278 ± 0.716

0.527 ± 0.228

3.645 ± 0.555
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski