Youngimonas vesicularis
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3652 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S3M7G0|A0A4S3M7G0_9RHOB FAA hydrolase family protein OS=Youngimonas vesicularis OX=1294297 GN=E7681_11360 PE=4 SV=1
MM1 pKa = 7.86 PNGNAYY7 pKa = 10.18 GHH9 pKa = 6.7 GNAAQNFHH17 pKa = 6.85 NGTANNPNNNGANGHH32 pKa = 6.44 SIAMDD37 pKa = 3.35 NVPFLSEE44 pKa = 4.1 TVFGFDD50 pKa = 3.87 LGEE53 pKa = 4.13 FSSTPGTNISYY64 pKa = 10.38 YY65 pKa = 10.14 SQGDD69 pKa = 4.06 FIATVPAWRR78 pKa = 11.84 GRR80 pKa = 11.84 VSHH83 pKa = 7.74 DD84 pKa = 2.83 IRR86 pKa = 11.84 DD87 pKa = 3.51 WDD89 pKa = 3.67 GDD91 pKa = 3.38 GDD93 pKa = 4.65 LEE95 pKa = 4.36 YY96 pKa = 10.85 STMNPGANMLIEE108 pKa = 4.94 HH109 pKa = 6.62 VDD111 pKa = 3.01 GDD113 pKa = 4.0 TFEE116 pKa = 4.5 FSGVSIEE123 pKa = 6.01 DD124 pKa = 3.56 YY125 pKa = 11.36 DD126 pKa = 4.01 NDD128 pKa = 4.1 GAGLINLRR136 pKa = 11.84 GTGLDD141 pKa = 3.31 GSSYY145 pKa = 11.07 GLYY148 pKa = 10.56 FEE150 pKa = 5.3 FDD152 pKa = 3.2 PRR154 pKa = 11.84 FNQLTIVGSVNSSDD168 pKa = 4.42 GTYY171 pKa = 9.73 EE172 pKa = 3.55 WQRR175 pKa = 11.84 FQNFDD180 pKa = 3.59 DD181 pKa = 5.09 AFDD184 pKa = 4.04 FVFTDD189 pKa = 3.75 HH190 pKa = 7.39 EE191 pKa = 4.42 LTQLSVYY198 pKa = 9.47 VRR200 pKa = 11.84 DD201 pKa = 3.53 MALDD205 pKa = 3.86 DD206 pKa = 5.03 FILVV210 pKa = 3.6
Molecular weight: 23.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.617
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.24
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.973
Patrickios 1.138
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|A0A4S3MBZ8|A0A4S3MBZ8_9RHOB PLP-dependent aminotransferase family protein OS=Youngimonas vesicularis OX=1294297 GN=E7681_10705 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 AGRR28 pKa = 11.84 KK29 pKa = 8.46 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3652
0
3652
1143577
28
2246
313.1
34.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.148 ± 0.056
0.883 ± 0.013
5.991 ± 0.035
5.766 ± 0.039
3.668 ± 0.023
8.767 ± 0.049
2.083 ± 0.02
5.151 ± 0.029
3.447 ± 0.038
10.123 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.893 ± 0.02
2.671 ± 0.023
5.056 ± 0.03
3.42 ± 0.021
6.529 ± 0.04
5.006 ± 0.028
5.453 ± 0.029
7.274 ± 0.032
1.392 ± 0.016
2.28 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here