Actinobacillus succinogenes (strain ATCC 55618 / DSM 22257 / 130Z)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Actinobacillus; Actinobacillus succinogenes

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2077 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A6VN03|RF3_ACTSZ Peptide chain release factor 3 OS=Actinobacillus succinogenes (strain ATCC 55618 / DSM 22257 / 130Z) OX=339671 GN=prfC PE=3 SV=1
MM1 pKa = 7.65AIVGLFYY8 pKa = 11.01GSDD11 pKa = 3.2TGNTEE16 pKa = 4.47NIAKK20 pKa = 9.24MIQKK24 pKa = 9.54QLGSEE29 pKa = 4.08LVDD32 pKa = 3.05IRR34 pKa = 11.84DD35 pKa = 3.46IAKK38 pKa = 8.47STKK41 pKa = 9.86EE42 pKa = 4.18DD43 pKa = 3.05IEE45 pKa = 5.8AYY47 pKa = 10.37DD48 pKa = 3.8FLLFGIPTWYY58 pKa = 10.39YY59 pKa = 11.58GEE61 pKa = 4.69AQCDD65 pKa = 3.26WDD67 pKa = 5.24DD68 pKa = 4.09FFPTLEE74 pKa = 4.91EE75 pKa = 4.17IDD77 pKa = 4.0FTDD80 pKa = 3.19KK81 pKa = 10.88LVAIFGCGDD90 pKa = 3.22QEE92 pKa = 4.63DD93 pKa = 4.32YY94 pKa = 11.51SEE96 pKa = 4.47YY97 pKa = 10.75FCDD100 pKa = 4.23AMGTIRR106 pKa = 11.84DD107 pKa = 3.59IVEE110 pKa = 3.75TRR112 pKa = 11.84GAIIVGNTSTEE123 pKa = 4.27GYY125 pKa = 8.71TFDD128 pKa = 4.91ASRR131 pKa = 11.84ALIDD135 pKa = 4.81DD136 pKa = 3.63NTFVGLCIDD145 pKa = 4.19EE146 pKa = 4.82DD147 pKa = 3.95RR148 pKa = 11.84QPEE151 pKa = 4.29LTSARR156 pKa = 11.84VEE158 pKa = 3.93NWVKK162 pKa = 10.57QIYY165 pKa = 10.63DD166 pKa = 3.86EE167 pKa = 4.37MCLAEE172 pKa = 4.39LAA174 pKa = 4.24

Molecular weight: 19.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A6VKA0|RL33_ACTSZ 50S ribosomal protein L33 OS=Actinobacillus succinogenes (strain ATCC 55618 / DSM 22257 / 130Z) OX=339671 GN=rpmG PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2077

0

2077

677060

37

5259

326.0

36.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.958 ± 0.059

1.039 ± 0.023

5.173 ± 0.05

6.124 ± 0.057

4.409 ± 0.043

7.01 ± 0.058

2.047 ± 0.026

6.7 ± 0.046

5.774 ± 0.045

10.369 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.031

4.605 ± 0.077

3.86 ± 0.039

4.381 ± 0.041

4.849 ± 0.054

5.61 ± 0.042

5.347 ± 0.064

6.968 ± 0.05

1.172 ± 0.02

3.108 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski