Pan troglodytes polyomavirus 5
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7QK02|K7QK02_9POLY Small T antigen OS=Pan troglodytes polyomavirus 5 OX=1891737 PE=4 SV=1
MM1 pKa = 7.48 GGVISIIVDD10 pKa = 4.11 IIEE13 pKa = 4.14 LTAEE17 pKa = 4.17 LTAATGFSVEE27 pKa = 4.42 AIINGEE33 pKa = 3.69 ALAAVEE39 pKa = 4.4 AQLSSLATVEE49 pKa = 4.17 GLTGVEE55 pKa = 4.21 ALASLGITTEE65 pKa = 4.02 QYY67 pKa = 11.46 SFLTSVPGMLSNAVGLGVVFQTVSGASALVTAGIATSYY105 pKa = 10.37 AKK107 pKa = 10.13 EE108 pKa = 3.83 VSVVNRR114 pKa = 11.84 NMAVIPWRR122 pKa = 11.84 DD123 pKa = 3.0 PDD125 pKa = 3.8 YY126 pKa = 11.55 YY127 pKa = 11.05 DD128 pKa = 3.18 ILFPGVQSFSYY139 pKa = 10.51 AIDD142 pKa = 4.31 VITDD146 pKa = 3.33 WSGSLFHH153 pKa = 7.47 SIGRR157 pKa = 11.84 YY158 pKa = 6.41 IWEE161 pKa = 4.33 SVTTEE166 pKa = 3.22 ARR168 pKa = 11.84 RR169 pKa = 11.84 QIGHH173 pKa = 7.72 ASNQVIVRR181 pKa = 11.84 GTHH184 pKa = 6.59 AFQDD188 pKa = 3.52 TLARR192 pKa = 11.84 ILEE195 pKa = 3.97 NARR198 pKa = 11.84 WVVQSGPTNVYY209 pKa = 10.52 NYY211 pKa = 10.02 LDD213 pKa = 3.31 NYY215 pKa = 9.21 YY216 pKa = 10.54 RR217 pKa = 11.84 QLPPVNPAQARR228 pKa = 11.84 QMYY231 pKa = 9.67 RR232 pKa = 11.84 RR233 pKa = 11.84 LGQKK237 pKa = 10.05 YY238 pKa = 8.61 RR239 pKa = 11.84 VTRR242 pKa = 11.84 DD243 pKa = 2.96 AQIPDD248 pKa = 3.76 RR249 pKa = 11.84 YY250 pKa = 10.07 NLEE253 pKa = 3.96 SSEE256 pKa = 4.37 TEE258 pKa = 3.6 SGEE261 pKa = 4.01 TVEE264 pKa = 5.56 FYY266 pKa = 10.85 KK267 pKa = 10.99 HH268 pKa = 6.39 PGGANQRR275 pKa = 11.84 VTPDD279 pKa = 2.51 WMLPLILGLYY289 pKa = 10.53 GDD291 pKa = 4.96 ITPTWDD297 pKa = 3.24 KK298 pKa = 10.89 YY299 pKa = 7.19 VHH301 pKa = 5.46 QVEE304 pKa = 5.23 EE305 pKa = 3.81 EE306 pKa = 4.08 DD307 pKa = 4.09 EE308 pKa = 4.32 YY309 pKa = 11.36 QKK311 pKa = 11.12 KK312 pKa = 9.61 KK313 pKa = 10.08 RR314 pKa = 11.84 RR315 pKa = 11.84 RR316 pKa = 11.84 VV317 pKa = 3.14
Molecular weight: 35.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.997
IPC2_protein 5.105
IPC_protein 5.029
Toseland 4.965
ProMoST 5.156
Dawson 5.003
Bjellqvist 5.143
Wikipedia 4.863
Rodwell 4.927
Grimsley 4.889
Solomon 5.003
Lehninger 4.965
Nozaki 5.13
DTASelect 5.258
Thurlkill 4.965
EMBOSS 4.914
Sillero 5.207
Patrickios 3.783
IPC_peptide 5.016
IPC2_peptide 5.207
IPC2.peptide.svr19 5.158
Protein with the highest isoelectric point:
>tr|K7QKH4|K7QKH4_9POLY Large T antigen OS=Pan troglodytes polyomavirus 5 OX=1891737 PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 6.07 KK3 pKa = 10.42 ILHH6 pKa = 5.9 KK7 pKa = 10.3 SEE9 pKa = 3.97 KK10 pKa = 10.05 KK11 pKa = 10.3 EE12 pKa = 4.08 LIALLEE18 pKa = 4.3 LPANEE23 pKa = 4.17 YY24 pKa = 11.37 GNYY27 pKa = 10.14 PLMKK31 pKa = 9.9 QQFKK35 pKa = 10.83 KK36 pKa = 9.53 MCLVYY41 pKa = 10.66 HH42 pKa = 6.93 PDD44 pKa = 3.32 KK45 pKa = 11.54 GGDD48 pKa = 3.64 GEE50 pKa = 4.8 KK51 pKa = 9.52 MRR53 pKa = 11.84 RR54 pKa = 11.84 LNALWQTFNTEE65 pKa = 3.95 LLEE68 pKa = 4.15 MRR70 pKa = 11.84 DD71 pKa = 3.36 NRR73 pKa = 11.84 FDD75 pKa = 3.59 MEE77 pKa = 4.25 QVRR80 pKa = 11.84 NMDD83 pKa = 3.71 LAIWEE88 pKa = 4.56 DD89 pKa = 4.02 LLTLKK94 pKa = 10.58 EE95 pKa = 4.29 VIDD98 pKa = 4.08 NFDD101 pKa = 3.26 EE102 pKa = 4.44 VFIKK106 pKa = 9.93 VFPSCFMRR114 pKa = 11.84 VAVHH118 pKa = 6.03 KK119 pKa = 8.59 CKK121 pKa = 10.7 CIVCMLRR128 pKa = 11.84 QQHH131 pKa = 7.2 LIMKK135 pKa = 6.63 EE136 pKa = 3.87 HH137 pKa = 6.45 KK138 pKa = 10.13 RR139 pKa = 11.84 CVLWGEE145 pKa = 4.4 CFCHH149 pKa = 6.77 KK150 pKa = 10.44 CYY152 pKa = 10.64 LDD154 pKa = 3.26 WYY156 pKa = 8.55 GLSGIEE162 pKa = 5.17 LHH164 pKa = 6.75 LIWWKK169 pKa = 10.62 RR170 pKa = 11.84 IVQNTPFIFMRR181 pKa = 11.84 VNVEE185 pKa = 3.87 DD186 pKa = 5.07 LYY188 pKa = 11.35 HH189 pKa = 6.21 KK190 pKa = 10.94 RR191 pKa = 11.84 NMM193 pKa = 3.91
Molecular weight: 23.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.059
IPC2_protein 7.337
IPC_protein 7.234
Toseland 6.912
ProMoST 7.761
Dawson 7.922
Bjellqvist 8.331
Wikipedia 7.834
Rodwell 7.936
Grimsley 6.956
Solomon 7.995
Lehninger 8.009
Nozaki 8.668
DTASelect 8.068
Thurlkill 8.112
EMBOSS 8.156
Sillero 8.463
Patrickios 4.291
IPC_peptide 7.98
IPC2_peptide 7.497
IPC2.peptide.svr19 7.394
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1626
193
733
406.5
46.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.781 ± 0.741
2.583 ± 0.624
5.535 ± 0.295
6.704 ± 0.357
4.49 ± 0.682
5.535 ± 0.936
1.845 ± 0.362
5.105 ± 0.469
7.134 ± 1.199
9.102 ± 0.549
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.014 ± 0.48
4.92 ± 0.295
5.474 ± 0.674
4.059 ± 0.291
4.551 ± 0.453
7.011 ± 0.839
5.843 ± 0.903
6.396 ± 1.161
1.292 ± 0.353
3.629 ± 0.439
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here