Capybara microvirus Cap1_SP_166
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W492|A0A4P8W492_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_166 OX=2585401 PE=4 SV=1
MM1 pKa = 7.45 VKK3 pKa = 9.25 MRR5 pKa = 11.84 SAVDD9 pKa = 3.87 FPTNAGSAFKK19 pKa = 10.31 IKK21 pKa = 9.13 YY22 pKa = 6.71 TPHH25 pKa = 6.79 VNEE28 pKa = 5.74 DD29 pKa = 3.3 GTIDD33 pKa = 3.57 LVEE36 pKa = 4.17 SEE38 pKa = 5.11 KK39 pKa = 10.98 INLQAEE45 pKa = 4.42 IDD47 pKa = 3.89 SHH49 pKa = 8.26 FDD51 pKa = 3.38 EE52 pKa = 6.02 VDD54 pKa = 3.38 MNSVMQRR61 pKa = 11.84 FGLGDD66 pKa = 3.77 TSAFSYY72 pKa = 10.94 NPLYY76 pKa = 11.0 GDD78 pKa = 3.25 TTVYY82 pKa = 10.15 PKK84 pKa = 10.25 TYY86 pKa = 11.13 AEE88 pKa = 4.0 MLQLQMDD95 pKa = 4.03 VDD97 pKa = 4.66 RR98 pKa = 11.84 YY99 pKa = 10.55 FMQLPTSIKK108 pKa = 9.85 EE109 pKa = 3.85 AYY111 pKa = 9.86 HH112 pKa = 5.45 YY113 pKa = 10.56 DD114 pKa = 3.49 KK115 pKa = 11.45 NQFFADD121 pKa = 3.17 IGSEE125 pKa = 3.63 RR126 pKa = 11.84 WNNLMNPAPASPEE139 pKa = 4.01 SPPEE143 pKa = 4.09 VITNEE148 pKa = 4.1 SS149 pKa = 3.07
Molecular weight: 16.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.371
IPC2_protein 4.406
IPC_protein 4.329
Toseland 4.164
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.19
Rodwell 4.164
Grimsley 4.075
Solomon 4.291
Lehninger 4.24
Nozaki 4.406
DTASelect 4.596
Thurlkill 4.177
EMBOSS 4.202
Sillero 4.444
Patrickios 3.503
IPC_peptide 4.291
IPC2_peptide 4.431
IPC2.peptide.svr19 4.376
Protein with the highest isoelectric point:
>tr|A0A4P8W815|A0A4P8W815_9VIRU Nonstructural protein OS=Capybara microvirus Cap1_SP_166 OX=2585401 PE=4 SV=1
MM1 pKa = 7.8 IEE3 pKa = 4.01 RR4 pKa = 11.84 KK5 pKa = 10.17 NEE7 pKa = 3.69 VFLLDD12 pKa = 3.02 ICSYY16 pKa = 9.71 KK17 pKa = 10.54 VYY19 pKa = 10.53 YY20 pKa = 10.69 SFVATIFILRR30 pKa = 11.84 CFMLVKK36 pKa = 10.35 VIITCARR43 pKa = 11.84 CGHH46 pKa = 6.48 RR47 pKa = 11.84 SNRR50 pKa = 11.84 KK51 pKa = 8.65 IRR53 pKa = 11.84 LGDD56 pKa = 3.53 YY57 pKa = 11.0 LMVRR61 pKa = 11.84 HH62 pKa = 6.79 DD63 pKa = 4.15 RR64 pKa = 11.84 QCSKK68 pKa = 10.82 CLSTDD73 pKa = 3.44 FDD75 pKa = 3.8 IFIEE79 pKa = 4.32 SFMEE83 pKa = 4.12 VV84 pKa = 2.86
Molecular weight: 10.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.835
IPC2_protein 7.834
IPC_protein 7.761
Toseland 7.234
ProMoST 8.258
Dawson 8.39
Bjellqvist 8.829
Wikipedia 8.302
Rodwell 8.39
Grimsley 7.161
Solomon 8.521
Lehninger 8.551
Nozaki 9.121
DTASelect 8.492
Thurlkill 8.551
EMBOSS 8.609
Sillero 8.916
Patrickios 4.024
IPC_peptide 8.521
IPC2_peptide 8.214
IPC2.peptide.svr19 8.232
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1487
83
569
247.8
28.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.994 ± 1.833
1.614 ± 0.722
7.128 ± 0.495
4.237 ± 0.434
5.514 ± 0.386
5.918 ± 0.581
3.295 ± 0.688
5.178 ± 0.824
4.506 ± 1.041
7.734 ± 0.85
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.757 ± 0.532
4.707 ± 0.802
5.38 ± 0.665
3.766 ± 0.312
4.976 ± 0.582
8.675 ± 1.618
5.044 ± 0.448
5.851 ± 0.522
1.21 ± 0.208
5.514 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here