Synechococcus sp. (strain RCC307)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5GVJ5|A5GVJ5_SYNR3 Distantly related to glycosyltransferase of family GT2 OS=Synechococcus sp. (strain RCC307) OX=316278 GN=SynRCC307_2001 PE=4 SV=1
MM1 pKa = 7.32HH2 pKa = 7.37LALRR6 pKa = 11.84EE7 pKa = 4.05EE8 pKa = 4.59FASEE12 pKa = 3.95EE13 pKa = 4.42TNGLVLGSCEE23 pKa = 4.17VLHH26 pKa = 7.31DD27 pKa = 3.9AAEE30 pKa = 4.3DD31 pKa = 3.99VACNLLNGLLYY42 pKa = 10.23PDD44 pKa = 4.82DD45 pKa = 3.93

Molecular weight: 4.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5GRR1|A5GRR1_SYNR3 Riboflavin synthase alpha chain OS=Synechococcus sp. (strain RCC307) OX=316278 GN=ribE PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 9.01RR4 pKa = 11.84TLGGTSRR11 pKa = 11.84KK12 pKa = 9.07RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.21TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.97RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.5

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2528

0

2528

697790

25

1915

276.0

30.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.993 ± 0.061

1.246 ± 0.018

4.949 ± 0.041

5.828 ± 0.054

3.085 ± 0.03

8.475 ± 0.059

2.023 ± 0.026

3.989 ± 0.04

2.65 ± 0.038

12.85 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.021 ± 0.022

2.598 ± 0.036

5.708 ± 0.041

5.457 ± 0.046

6.89 ± 0.053

6.369 ± 0.04

4.327 ± 0.037

6.897 ± 0.043

1.82 ± 0.026

1.824 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski