Amino acid dipepetide frequency for Synechococcus sp. (strain RCC307)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.421AlaAla: 14.421 ± 0.194	1.525AlaCys: 1.525 ± 0.053	5.744AlaAsp: 5.744 ± 0.106	7.029AlaGlu: 7.029 ± 0.111	3.074AlaPhe: 3.074 ± 0.064	9.185AlaGly: 9.185 ± 0.127	1.784AlaHis: 1.784 ± 0.053	5.082AlaIle: 5.082 ± 0.11	2.934AlaLys: 2.934 ± 0.077	14.347AlaLeu: 14.347 ± 0.214	3.004AlaMet: 3.004 ± 0.069	2.558AlaAsn: 2.558 ± 0.073	5.231AlaPro: 5.231 ± 0.106	4.887AlaGln: 4.887 ± 0.089	5.781AlaArg: 5.781 ± 0.097	6.896AlaSer: 6.896 ± 0.119	4.49AlaThr: 4.49 ± 0.091	8.325AlaVal: 8.325 ± 0.125	1.721AlaTrp: 1.721 ± 0.051	1.916AlaTyr: 1.916 ± 0.066	0.0AlaXaa: 0.0 ± 0.0
Cys	1.0CysAla: 1.0 ± 0.039	0.278CysCys: 0.278 ± 0.021	0.681CysAsp: 0.681 ± 0.032	0.639CysGlu: 0.639 ± 0.03	0.473CysPhe: 0.473 ± 0.027	1.296CysGly: 1.296 ± 0.047	0.341CysHis: 0.341 ± 0.024	0.496CysIle: 0.496 ± 0.029	0.241CysLys: 0.241 ± 0.017	1.339CysLeu: 1.339 ± 0.043	0.182CysMet: 0.182 ± 0.017	0.324CysAsn: 0.324 ± 0.02	0.745CysPro: 0.745 ± 0.03	0.615CysGln: 0.615 ± 0.031	1.056CysArg: 1.056 ± 0.047	0.987CysSer: 0.987 ± 0.038	0.516CysThr: 0.516 ± 0.027	0.699CysVal: 0.699 ± 0.035	0.314CysTrp: 0.314 ± 0.02	0.236CysTyr: 0.236 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	5.096AspAla: 5.096 ± 0.098	0.612AspCys: 0.612 ± 0.027	2.439AspAsp: 2.439 ± 0.07	2.744AspGlu: 2.744 ± 0.062	1.541AspPhe: 1.541 ± 0.057	4.828AspGly: 4.828 ± 0.1	1.083AspHis: 1.083 ± 0.048	1.492AspIle: 1.492 ± 0.052	1.085AspLys: 1.085 ± 0.047	6.544AspLeu: 6.544 ± 0.103	0.717AspMet: 0.717 ± 0.031	1.202AspAsn: 1.202 ± 0.044	3.927AspPro: 3.927 ± 0.096	2.929AspGln: 2.929 ± 0.059	3.442AspArg: 3.442 ± 0.079	2.601AspSer: 2.601 ± 0.06	1.912AspThr: 1.912 ± 0.065	3.124AspVal: 3.124 ± 0.074	1.005AspTrp: 1.005 ± 0.041	1.169AspTyr: 1.169 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	7.519GluAla: 7.519 ± 0.115	0.416GluCys: 0.416 ± 0.028	2.154GluAsp: 2.154 ± 0.06	3.256GluGlu: 3.256 ± 0.075	1.281GluPhe: 1.281 ± 0.046	4.349GluGly: 4.349 ± 0.082	1.31GluHis: 1.31 ± 0.041	2.337GluIle: 2.337 ± 0.053	1.562GluLys: 1.562 ± 0.055	8.778GluLeu: 8.778 ± 0.127	1.03GluMet: 1.03 ± 0.044	1.146GluAsn: 1.146 ± 0.039	3.544GluPro: 3.544 ± 0.076	4.239GluGln: 4.239 ± 0.093	5.151GluArg: 5.151 ± 0.106	2.832GluSer: 2.832 ± 0.065	2.297GluThr: 2.297 ± 0.063	3.753GluVal: 3.753 ± 0.075	0.702GluTrp: 0.702 ± 0.037	0.622GluTyr: 0.622 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.068PheAla: 3.068 ± 0.072	0.556PheCys: 0.556 ± 0.029	1.94PheAsp: 1.94 ± 0.043	1.854PheGlu: 1.854 ± 0.047	1.122PhePhe: 1.122 ± 0.052	3.005PheGly: 3.005 ± 0.075	0.678PheHis: 0.678 ± 0.033	1.225PheIle: 1.225 ± 0.043	0.797PheLys: 0.797 ± 0.041	3.075PheLeu: 3.075 ± 0.074	0.555PheMet: 0.555 ± 0.028	1.103PheAsn: 1.103 ± 0.04	1.344PhePro: 1.344 ± 0.044	1.27PheGln: 1.27 ± 0.048	1.93PheArg: 1.93 ± 0.061	2.509PheSer: 2.509 ± 0.064	1.546PheThr: 1.546 ± 0.048	1.933PheVal: 1.933 ± 0.058	0.588PheTrp: 0.588 ± 0.034	0.754PheTyr: 0.754 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	7.843GlyAla: 7.843 ± 0.133	1.389GlyCys: 1.389 ± 0.048	4.348GlyAsp: 4.348 ± 0.093	4.649GlyGlu: 4.649 ± 0.081	3.31GlyPhe: 3.31 ± 0.071	7.294GlyGly: 7.294 ± 0.182	1.763GlyHis: 1.763 ± 0.048	3.832GlyIle: 3.832 ± 0.088	2.28GlyLys: 2.28 ± 0.067	10.985GlyLeu: 10.985 ± 0.138	2.075GlyMet: 2.075 ± 0.054	2.227GlyAsn: 2.227 ± 0.094	3.576GlyPro: 3.576 ± 0.075	3.924GlyGln: 3.924 ± 0.082	5.741GlyArg: 5.741 ± 0.11	5.777GlySer: 5.777 ± 0.111	3.781GlyThr: 3.781 ± 0.101	6.164GlyVal: 6.164 ± 0.103	1.854GlyTrp: 1.854 ± 0.054	1.935GlyTyr: 1.935 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.589HisAla: 1.589 ± 0.047	0.37HisCys: 0.37 ± 0.026	0.924HisAsp: 0.924 ± 0.039	0.837HisGlu: 0.837 ± 0.034	0.695HisPhe: 0.695 ± 0.031	1.993HisGly: 1.993 ± 0.052	0.837HisHis: 0.837 ± 0.041	0.579HisIle: 0.579 ± 0.035	0.436HisLys: 0.436 ± 0.025	2.459HisLeu: 2.459 ± 0.067	0.282HisMet: 0.282 ± 0.021	0.552HisAsn: 0.552 ± 0.027	1.548HisPro: 1.548 ± 0.046	1.251HisGln: 1.251 ± 0.046	1.678HisArg: 1.678 ± 0.056	1.221HisSer: 1.221 ± 0.04	0.817HisThr: 0.817 ± 0.037	1.033HisVal: 1.033 ± 0.044	0.633HisTrp: 0.633 ± 0.029	0.496HisTyr: 0.496 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	4.874IleAla: 4.874 ± 0.092	0.573IleCys: 0.573 ± 0.028	2.688IleAsp: 2.688 ± 0.068	2.752IleGlu: 2.752 ± 0.067	1.046IlePhe: 1.046 ± 0.051	4.03IleGly: 4.03 ± 0.105	0.837IleHis: 0.837 ± 0.037	1.151IleIle: 1.151 ± 0.052	1.28IleLys: 1.28 ± 0.049	2.975IleLeu: 2.975 ± 0.078	0.364IleMet: 0.364 ± 0.025	1.564IleAsn: 1.564 ± 0.055	2.362IlePro: 2.362 ± 0.06	1.584IleGln: 1.584 ± 0.055	2.485IleArg: 2.485 ± 0.058	3.071IleSer: 3.071 ± 0.068	2.518IleThr: 2.518 ± 0.074	2.283IleVal: 2.283 ± 0.064	0.642IleTrp: 0.642 ± 0.031	0.811IleTyr: 0.811 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	3.501LysAla: 3.501 ± 0.088	0.176LysCys: 0.176 ± 0.016	1.283LysAsp: 1.283 ± 0.048	1.5LysGlu: 1.5 ± 0.053	0.556LysPhe: 0.556 ± 0.033	2.184LysGly: 2.184 ± 0.071	0.431LysHis: 0.431 ± 0.027	0.936LysIle: 0.936 ± 0.045	0.867LysLys: 0.867 ± 0.041	3.134LysLeu: 3.134 ± 0.07	0.401LysMet: 0.401 ± 0.024	0.655LysAsn: 0.655 ± 0.043	1.886LysPro: 1.886 ± 0.067	1.582LysGln: 1.582 ± 0.049	2.094LysArg: 2.094 ± 0.064	1.424LysSer: 1.424 ± 0.048	1.337LysThr: 1.337 ± 0.046	1.874LysVal: 1.874 ± 0.061	0.245LysTrp: 0.245 ± 0.019	0.434LysTyr: 0.434 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	14.328LeuAla: 14.328 ± 0.18	1.339LeuCys: 1.339 ± 0.046	6.162LeuAsp: 6.162 ± 0.099	8.675LeuGlu: 8.675 ± 0.151	3.491LeuPhe: 3.491 ± 0.078	9.85LeuGly: 9.85 ± 0.141	2.388LeuHis: 2.388 ± 0.06	5.103LeuIle: 5.103 ± 0.097	3.869LeuLys: 3.869 ± 0.073	17.204LeuLeu: 17.204 ± 0.295	2.717LeuMet: 2.717 ± 0.063	3.353LeuAsn: 3.353 ± 0.084	7.535LeuPro: 7.535 ± 0.121	7.743LeuGln: 7.743 ± 0.135	8.59LeuArg: 8.59 ± 0.141	8.028LeuSer: 8.028 ± 0.11	5.13LeuThr: 5.13 ± 0.089	9.041LeuVal: 9.041 ± 0.143	2.067LeuTrp: 2.067 ± 0.066	1.887LeuTyr: 1.887 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	3.323MetAla: 3.323 ± 0.073	0.118MetCys: 0.118 ± 0.015	0.985MetAsp: 0.985 ± 0.041	1.191MetGlu: 1.191 ± 0.039	0.43MetPhe: 0.43 ± 0.027	1.829MetGly: 1.829 ± 0.056	0.345MetHis: 0.345 ± 0.023	0.651MetIle: 0.651 ± 0.033	0.578MetLys: 0.578 ± 0.028	2.048MetLeu: 2.048 ± 0.057	0.292MetMet: 0.292 ± 0.022	0.54MetAsn: 0.54 ± 0.028	1.318MetPro: 1.318 ± 0.04	0.914MetGln: 0.914 ± 0.031	1.379MetArg: 1.379 ± 0.049	1.265MetSer: 1.265 ± 0.044	1.098MetThr: 1.098 ± 0.042	1.596MetVal: 1.596 ± 0.047	0.122MetTrp: 0.122 ± 0.014	0.189MetTyr: 0.189 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.592AsnAla: 2.592 ± 0.07	0.331AsnCys: 0.331 ± 0.023	1.4AsnAsp: 1.4 ± 0.07	1.115AsnGlu: 1.115 ± 0.04	0.903AsnPhe: 0.903 ± 0.041	2.435AsnGly: 2.435 ± 0.068	0.556AsnHis: 0.556 ± 0.029	0.906AsnIle: 0.906 ± 0.043	0.735AsnLys: 0.735 ± 0.03	3.04AsnLeu: 3.04 ± 0.063	0.355AsnMet: 0.355 ± 0.022	0.834AsnAsn: 0.834 ± 0.041	2.055AsnPro: 2.055 ± 0.053	1.265AsnGln: 1.265 ± 0.044	1.879AsnArg: 1.879 ± 0.057	1.728AsnSer: 1.728 ± 0.061	1.274AsnThr: 1.274 ± 0.05	1.469AsnVal: 1.469 ± 0.049	0.503AsnTrp: 0.503 ± 0.027	0.603AsnTyr: 0.603 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	6.638ProAla: 6.638 ± 0.112	0.593ProCys: 0.593 ± 0.029	3.008ProAsp: 3.008 ± 0.063	4.229ProGlu: 4.229 ± 0.09	1.763ProPhe: 1.763 ± 0.051	4.632ProGly: 4.632 ± 0.076	1.033ProHis: 1.033 ± 0.048	2.193ProIle: 2.193 ± 0.057	1.542ProLys: 1.542 ± 0.052	7.444ProLeu: 7.444 ± 0.116	1.33ProMet: 1.33 ± 0.049	1.344ProAsn: 1.344 ± 0.048	2.921ProPro: 2.921 ± 0.082	3.007ProGln: 3.007 ± 0.07	2.865ProArg: 2.865 ± 0.064	3.637ProSer: 3.637 ± 0.083	2.541ProThr: 2.541 ± 0.058	4.198ProVal: 4.198 ± 0.081	1.121ProTrp: 1.121 ± 0.041	1.046ProTyr: 1.046 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	6.486GlnAla: 6.486 ± 0.115	0.451GlnCys: 0.451 ± 0.023	1.69GlnAsp: 1.69 ± 0.051	2.872GlnGlu: 2.872 ± 0.067	1.253GlnPhe: 1.253 ± 0.043	4.122GlnGly: 4.122 ± 0.085	1.146GlnHis: 1.146 ± 0.045	1.741GlnIle: 1.741 ± 0.052	1.142GlnLys: 1.142 ± 0.042	8.365GlnLeu: 8.365 ± 0.133	0.82GlnMet: 0.82 ± 0.031	0.982GlnAsn: 0.982 ± 0.036	3.465GlnPro: 3.465 ± 0.075	4.994GlnGln: 4.994 ± 0.131	5.321GlnArg: 5.321 ± 0.107	2.723GlnSer: 2.723 ± 0.066	1.731GlnThr: 1.731 ± 0.06	3.538GlnVal: 3.538 ± 0.08	1.197GlnTrp: 1.197 ± 0.046	0.533GlnTyr: 0.533 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	5.327ArgAla: 5.327 ± 0.099	1.103ArgCys: 1.103 ± 0.046	3.283ArgAsp: 3.283 ± 0.07	3.474ArgGlu: 3.474 ± 0.068	2.878ArgPhe: 2.878 ± 0.068	4.633ArgGly: 4.633 ± 0.084	1.705ArgHis: 1.705 ± 0.048	2.892ArgIle: 2.892 ± 0.074	1.661ArgLys: 1.661 ± 0.055	9.638ArgLeu: 9.638 ± 0.147	1.495ArgMet: 1.495 ± 0.047	1.735ArgAsn: 1.735 ± 0.051	3.538ArgPro: 3.538 ± 0.091	4.781ArgGln: 4.781 ± 0.104	5.97ArgArg: 5.97 ± 0.121	4.741ArgSer: 4.741 ± 0.083	2.495ArgThr: 2.495 ± 0.068	3.99ArgVal: 3.99 ± 0.081	1.929ArgTrp: 1.929 ± 0.055	1.631ArgTyr: 1.631 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	6.473SerAla: 6.473 ± 0.093	0.883SerCys: 0.883 ± 0.037	3.389SerAsp: 3.389 ± 0.066	3.424SerGlu: 3.424 ± 0.077	2.198SerPhe: 2.198 ± 0.056	6.354SerGly: 6.354 ± 0.109	1.321SerHis: 1.321 ± 0.04	2.496SerIle: 2.496 ± 0.067	1.68SerLys: 1.68 ± 0.054	7.59SerLeu: 7.59 ± 0.102	1.392SerMet: 1.392 ± 0.055	1.826SerAsn: 1.826 ± 0.059	3.351SerPro: 3.351 ± 0.08	2.825SerGln: 2.825 ± 0.061	4.133SerArg: 4.133 ± 0.074	4.831SerSer: 4.831 ± 0.101	3.085SerThr: 3.085 ± 0.062	3.802SerVal: 3.802 ± 0.082	1.353SerTrp: 1.353 ± 0.048	1.284SerTyr: 1.284 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	5.198ThrAla: 5.198 ± 0.097	0.454ThrCys: 0.454 ± 0.026	2.022ThrAsp: 2.022 ± 0.057	2.131ThrGlu: 2.131 ± 0.051	1.306ThrPhe: 1.306 ± 0.044	3.978ThrGly: 3.978 ± 0.081	0.77ThrHis: 0.77 ± 0.032	1.966ThrIle: 1.966 ± 0.063	1.116ThrLys: 1.116 ± 0.041	5.066ThrLeu: 5.066 ± 0.101	0.768ThrMet: 0.768 ± 0.032	1.177ThrAsn: 1.177 ± 0.047	3.163ThrPro: 3.163 ± 0.069	1.489ThrGln: 1.489 ± 0.046	2.378ThrArg: 2.378 ± 0.065	2.875ThrSer: 2.875 ± 0.072	2.535ThrThr: 2.535 ± 0.074	3.305ThrVal: 3.305 ± 0.072	0.588ThrTrp: 0.588 ± 0.029	0.986ThrTyr: 0.986 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	7.562ValAla: 7.562 ± 0.112	0.734ValCys: 0.734 ± 0.033	3.617ValAsp: 3.617 ± 0.077	4.276ValGlu: 4.276 ± 0.087	2.324ValPhe: 2.324 ± 0.053	5.312ValGly: 5.312 ± 0.091	1.181ValHis: 1.181 ± 0.043	3.331ValIle: 3.331 ± 0.082	1.776ValLys: 1.776 ± 0.066	9.441ValLeu: 9.441 ± 0.145	1.668ValMet: 1.668 ± 0.053	1.859ValAsn: 1.859 ± 0.058	3.517ValPro: 3.517 ± 0.077	3.065ValGln: 3.065 ± 0.069	3.967ValArg: 3.967 ± 0.078	4.056ValSer: 4.056 ± 0.075	2.83ValThr: 2.83 ± 0.069	6.634ValVal: 6.634 ± 0.129	0.825ValTrp: 0.825 ± 0.04	0.996ValTyr: 0.996 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.389TrpAla: 1.389 ± 0.05	0.261TrpCys: 0.261 ± 0.019	0.764TrpAsp: 0.764 ± 0.04	0.675TrpGlu: 0.675 ± 0.032	0.632TrpPhe: 0.632 ± 0.031	1.346TrpGly: 1.346 ± 0.05	0.416TrpHis: 0.416 ± 0.025	0.847TrpIle: 0.847 ± 0.042	0.426TrpLys: 0.426 ± 0.025	2.969TrpLeu: 2.969 ± 0.073	0.463TrpMet: 0.463 ± 0.025	0.507TrpAsn: 0.507 ± 0.026	1.073TrpPro: 1.073 ± 0.049	1.38TrpGln: 1.38 ± 0.042	1.513TrpArg: 1.513 ± 0.05	1.179TrpSer: 1.179 ± 0.037	0.59TrpThr: 0.59 ± 0.027	1.056TrpVal: 1.056 ± 0.041	0.431TrpTrp: 0.431 ± 0.029	0.278TrpTyr: 0.278 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.704TyrAla: 1.704 ± 0.055	0.297TyrCys: 0.297 ± 0.022	0.97TyrAsp: 0.97 ± 0.038	0.957TyrGlu: 0.957 ± 0.043	0.579TyrPhe: 0.579 ± 0.028	2.091TyrGly: 2.091 ± 0.059	0.305TyrHis: 0.305 ± 0.02	0.633TyrIle: 0.633 ± 0.035	0.497TyrLys: 0.497 ± 0.031	2.062TyrLeu: 2.062 ± 0.057	0.3TyrMet: 0.3 ± 0.021	0.49TyrAsn: 0.49 ± 0.026	0.985TyrPro: 0.985 ± 0.035	0.828TyrGln: 0.828 ± 0.035	1.546TyrArg: 1.546 ± 0.043	1.307TyrSer: 1.307 ± 0.049	0.745TyrThr: 0.745 ± 0.039	1.154TyrVal: 1.154 ± 0.041	0.357TyrTrp: 0.357 ± 0.022	0.433TyrTyr: 0.433 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2528 proteins (697790 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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