Croceibacter phage P2559Y

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8CQM7|W8CQM7_9CAUD Putative tail protein OS=Croceibacter phage P2559Y OX=1327037 GN=P2559Y_0004 PE=4 SV=1
MM1 pKa = 7.67NFGTPQEE8 pKa = 4.7MIDD11 pKa = 3.83EE12 pKa = 4.64HH13 pKa = 7.69RR14 pKa = 11.84DD15 pKa = 3.36TYY17 pKa = 11.07PDD19 pKa = 3.7EE20 pKa = 4.94NSGCFPILLLIVFILVVLCITKK42 pKa = 10.42

Molecular weight: 4.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8CPL4|W8CPL4_9CAUD Uncharacterized protein OS=Croceibacter phage P2559Y OX=1327037 GN=P2559Y_0037 PE=4 SV=1
MM1 pKa = 7.26QSKK4 pKa = 9.33KK5 pKa = 10.12QSWVEE10 pKa = 3.61AFTNTFVGFVISLTAIFLILPLFGVEE36 pKa = 3.93STPIKK41 pKa = 10.72NVGITLCFTVISILRR56 pKa = 11.84GYY58 pKa = 10.02LIRR61 pKa = 11.84RR62 pKa = 11.84FFNKK66 pKa = 9.42KK67 pKa = 9.2HH68 pKa = 5.88SKK70 pKa = 9.87

Molecular weight: 8.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

13348

40

1248

261.7

29.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.435 ± 0.631

0.944 ± 0.126

6.555 ± 0.263

6.75 ± 0.33

4.577 ± 0.249

6.39 ± 0.229

1.648 ± 0.17

7.312 ± 0.288

8.338 ± 0.383

8.376 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.985 ± 0.133

5.701 ± 0.285

3.701 ± 0.271

3.663 ± 0.203

3.873 ± 0.213

5.911 ± 0.221

6.398 ± 0.335

6.008 ± 0.239

1.416 ± 0.104

4.016 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski