Amino acid dipepetide frequency for Croceibacter phage P2559Y

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.844AlaAla: 5.844 ± 1.377
0.524AlaCys: 0.524 ± 0.218
4.42AlaAsp: 4.42 ± 0.609
5.469AlaGlu: 5.469 ± 1.079
2.398AlaPhe: 2.398 ± 0.458
3.072AlaGly: 3.072 ± 0.565
1.424AlaHis: 1.424 ± 0.438
4.87AlaIle: 4.87 ± 0.717
4.945AlaLys: 4.945 ± 0.893
5.32AlaLeu: 5.32 ± 1.241
1.498AlaMet: 1.498 ± 0.424
3.821AlaAsn: 3.821 ± 0.468
1.424AlaPro: 1.424 ± 0.526
2.472AlaGln: 2.472 ± 0.383
2.248AlaArg: 2.248 ± 0.348
2.398AlaSer: 2.398 ± 0.362
4.87AlaThr: 4.87 ± 0.794
4.196AlaVal: 4.196 ± 0.522
0.974AlaTrp: 0.974 ± 0.249
2.173AlaTyr: 2.173 ± 0.423
0.0AlaXaa: 0.0 ± 0.0
Cys
0.45CysAla: 0.45 ± 0.19
0.075CysCys: 0.075 ± 0.083
0.674CysAsp: 0.674 ± 0.279
0.899CysGlu: 0.899 ± 0.279
0.3CysPhe: 0.3 ± 0.139
0.674CysGly: 0.674 ± 0.228
0.0CysHis: 0.0 ± 0.0
0.45CysIle: 0.45 ± 0.156
0.899CysLys: 0.899 ± 0.243
1.124CysLeu: 1.124 ± 0.296
0.3CysMet: 0.3 ± 0.154
0.375CysAsn: 0.375 ± 0.155
0.45CysPro: 0.45 ± 0.239
0.3CysGln: 0.3 ± 0.149
0.45CysArg: 0.45 ± 0.157
0.599CysSer: 0.599 ± 0.223
0.45CysThr: 0.45 ± 0.207
0.524CysVal: 0.524 ± 0.174
0.075CysTrp: 0.075 ± 0.075
0.375CysTyr: 0.375 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
3.521AspAla: 3.521 ± 0.608
0.674AspCys: 0.674 ± 0.267
4.346AspAsp: 4.346 ± 0.801
4.046AspGlu: 4.046 ± 0.61
4.046AspPhe: 4.046 ± 0.674
4.121AspGly: 4.121 ± 0.476
1.124AspHis: 1.124 ± 0.314
4.271AspIle: 4.271 ± 0.587
4.645AspLys: 4.645 ± 0.544
6.743AspLeu: 6.743 ± 0.937
1.349AspMet: 1.349 ± 0.276
3.072AspAsn: 3.072 ± 0.507
2.173AspPro: 2.173 ± 0.447
2.098AspGln: 2.098 ± 0.438
2.398AspArg: 2.398 ± 0.335
3.821AspSer: 3.821 ± 0.557
3.372AspThr: 3.372 ± 0.471
5.394AspVal: 5.394 ± 0.585
1.498AspTrp: 1.498 ± 0.41
2.847AspTyr: 2.847 ± 0.573
0.0AspXaa: 0.0 ± 0.0
Glu
5.245GluAla: 5.245 ± 0.924
0.599GluCys: 0.599 ± 0.228
2.398GluAsp: 2.398 ± 0.457
3.222GluGlu: 3.222 ± 0.615
3.821GluPhe: 3.821 ± 0.39
3.596GluGly: 3.596 ± 0.648
1.274GluHis: 1.274 ± 0.339
6.219GluIle: 6.219 ± 0.867
6.069GluLys: 6.069 ± 0.963
5.394GluLeu: 5.394 ± 0.599
1.498GluMet: 1.498 ± 0.286
4.72GluAsn: 4.72 ± 0.8
1.498GluPro: 1.498 ± 0.373
1.798GluGln: 1.798 ± 0.325
3.147GluArg: 3.147 ± 0.549
3.596GluSer: 3.596 ± 0.424
5.095GluThr: 5.095 ± 0.853
4.87GluVal: 4.87 ± 0.623
0.674GluTrp: 0.674 ± 0.251
2.772GluTyr: 2.772 ± 0.378
0.0GluXaa: 0.0 ± 0.0
Phe
3.671PheAla: 3.671 ± 0.434
0.524PheCys: 0.524 ± 0.189
3.372PheAsp: 3.372 ± 0.423
3.596PheGlu: 3.596 ± 0.405
1.648PhePhe: 1.648 ± 0.351
2.772PheGly: 2.772 ± 0.446
0.749PheHis: 0.749 ± 0.249
2.847PheIle: 2.847 ± 0.492
3.297PheLys: 3.297 ± 0.443
3.446PheLeu: 3.446 ± 0.559
0.749PheMet: 0.749 ± 0.264
2.922PheAsn: 2.922 ± 0.62
2.023PhePro: 2.023 ± 0.391
0.674PheGln: 0.674 ± 0.191
1.274PheArg: 1.274 ± 0.294
2.697PheSer: 2.697 ± 0.417
3.372PheThr: 3.372 ± 0.663
2.772PheVal: 2.772 ± 0.631
1.124PheTrp: 1.124 ± 0.239
2.248PheTyr: 2.248 ± 0.472
0.0PheXaa: 0.0 ± 0.0
Gly
3.072GlyAla: 3.072 ± 0.576
0.599GlyCys: 0.599 ± 0.241
3.821GlyAsp: 3.821 ± 0.561
2.997GlyGlu: 2.997 ± 0.508
2.922GlyPhe: 2.922 ± 0.392
6.069GlyGly: 6.069 ± 0.735
1.199GlyHis: 1.199 ± 0.317
4.42GlyIle: 4.42 ± 0.476
5.245GlyLys: 5.245 ± 0.686
5.32GlyLeu: 5.32 ± 0.542
1.049GlyMet: 1.049 ± 0.265
3.746GlyAsn: 3.746 ± 0.684
0.45GlyPro: 0.45 ± 0.174
2.098GlyGln: 2.098 ± 0.47
2.622GlyArg: 2.622 ± 0.34
3.297GlySer: 3.297 ± 0.447
5.994GlyThr: 5.994 ± 0.926
4.346GlyVal: 4.346 ± 0.423
1.498GlyTrp: 1.498 ± 0.384
3.147GlyTyr: 3.147 ± 0.478
0.0GlyXaa: 0.0 ± 0.0
His
1.049HisAla: 1.049 ± 0.246
0.375HisCys: 0.375 ± 0.162
1.274HisAsp: 1.274 ± 0.301
0.749HisGlu: 0.749 ± 0.246
0.674HisPhe: 0.674 ± 0.298
1.124HisGly: 1.124 ± 0.286
0.45HisHis: 0.45 ± 0.206
1.723HisIle: 1.723 ± 0.336
0.599HisLys: 0.599 ± 0.206
1.648HisLeu: 1.648 ± 0.395
0.0HisMet: 0.0 ± 0.0
1.049HisAsn: 1.049 ± 0.222
0.899HisPro: 0.899 ± 0.307
0.749HisGln: 0.749 ± 0.265
0.674HisArg: 0.674 ± 0.185
1.274HisSer: 1.274 ± 0.271
0.749HisThr: 0.749 ± 0.164
0.524HisVal: 0.524 ± 0.187
0.225HisTrp: 0.225 ± 0.135
0.674HisTyr: 0.674 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
4.795IleAla: 4.795 ± 0.584
0.674IleCys: 0.674 ± 0.213
5.619IleAsp: 5.619 ± 0.605
5.394IleGlu: 5.394 ± 0.599
2.248IlePhe: 2.248 ± 0.493
4.72IleGly: 4.72 ± 0.63
1.648IleHis: 1.648 ± 0.371
5.02IleIle: 5.02 ± 0.77
6.368IleLys: 6.368 ± 0.9
6.294IleLeu: 6.294 ± 0.827
1.049IleMet: 1.049 ± 0.262
4.42IleAsn: 4.42 ± 0.442
2.922IlePro: 2.922 ± 0.495
2.772IleGln: 2.772 ± 0.37
2.697IleArg: 2.697 ± 0.451
3.821IleSer: 3.821 ± 0.553
4.945IleThr: 4.945 ± 0.65
4.196IleVal: 4.196 ± 0.732
0.899IleTrp: 0.899 ± 0.383
2.622IleTyr: 2.622 ± 0.442
0.0IleXaa: 0.0 ± 0.0
Lys
6.968LysAla: 6.968 ± 1.563
0.225LysCys: 0.225 ± 0.196
5.17LysAsp: 5.17 ± 0.54
5.17LysGlu: 5.17 ± 0.645
2.922LysPhe: 2.922 ± 0.487
4.271LysGly: 4.271 ± 0.479
1.124LysHis: 1.124 ± 0.278
7.118LysIle: 7.118 ± 0.577
8.316LysLys: 8.316 ± 1.246
6.668LysLeu: 6.668 ± 0.792
1.873LysMet: 1.873 ± 0.446
5.17LysAsn: 5.17 ± 0.609
2.847LysPro: 2.847 ± 0.512
3.222LysGln: 3.222 ± 0.748
2.697LysArg: 2.697 ± 0.464
4.795LysSer: 4.795 ± 0.606
5.17LysThr: 5.17 ± 0.592
4.046LysVal: 4.046 ± 0.486
1.349LysTrp: 1.349 ± 0.391
4.196LysTyr: 4.196 ± 0.397
0.0LysXaa: 0.0 ± 0.0
Leu
4.046LeuAla: 4.046 ± 0.616
0.974LeuCys: 0.974 ± 0.313
5.994LeuAsp: 5.994 ± 0.736
6.219LeuGlu: 6.219 ± 0.906
4.42LeuPhe: 4.42 ± 0.601
5.17LeuGly: 5.17 ± 0.49
1.199LeuHis: 1.199 ± 0.327
5.245LeuIle: 5.245 ± 0.523
6.968LeuLys: 6.968 ± 0.703
6.294LeuLeu: 6.294 ± 0.843
1.798LeuMet: 1.798 ± 0.419
5.02LeuAsn: 5.02 ± 0.669
3.446LeuPro: 3.446 ± 0.431
4.046LeuGln: 4.046 ± 0.488
3.372LeuArg: 3.372 ± 0.391
5.469LeuSer: 5.469 ± 0.757
4.795LeuThr: 4.795 ± 0.611
5.469LeuVal: 5.469 ± 0.729
0.974LeuTrp: 0.974 ± 0.222
2.847LeuTyr: 2.847 ± 0.506
0.0LeuXaa: 0.0 ± 0.0
Met
1.199MetAla: 1.199 ± 0.271
0.225MetCys: 0.225 ± 0.126
1.349MetAsp: 1.349 ± 0.261
1.199MetGlu: 1.199 ± 0.338
0.674MetPhe: 0.674 ± 0.177
2.023MetGly: 2.023 ± 0.266
0.524MetHis: 0.524 ± 0.201
1.124MetIle: 1.124 ± 0.285
1.573MetLys: 1.573 ± 0.38
2.023MetLeu: 2.023 ± 0.331
0.375MetMet: 0.375 ± 0.139
0.974MetAsn: 0.974 ± 0.318
0.45MetPro: 0.45 ± 0.146
1.349MetGln: 1.349 ± 0.317
0.524MetArg: 0.524 ± 0.158
1.948MetSer: 1.948 ± 0.369
0.749MetThr: 0.749 ± 0.262
0.674MetVal: 0.674 ± 0.171
0.15MetTrp: 0.15 ± 0.091
0.749MetTyr: 0.749 ± 0.234
0.0MetXaa: 0.0 ± 0.0
Asn
3.297AsnAla: 3.297 ± 0.558
0.524AsnCys: 0.524 ± 0.231
2.398AsnAsp: 2.398 ± 0.42
3.372AsnGlu: 3.372 ± 0.464
2.697AsnPhe: 2.697 ± 0.426
4.346AsnGly: 4.346 ± 0.628
0.749AsnHis: 0.749 ± 0.209
4.121AsnIle: 4.121 ± 0.554
5.095AsnLys: 5.095 ± 0.488
4.72AsnLeu: 4.72 ± 0.539
0.749AsnMet: 0.749 ± 0.216
3.446AsnAsn: 3.446 ± 0.446
4.196AsnPro: 4.196 ± 0.604
1.648AsnGln: 1.648 ± 0.423
2.622AsnArg: 2.622 ± 0.462
3.072AsnSer: 3.072 ± 0.45
3.596AsnThr: 3.596 ± 0.645
3.072AsnVal: 3.072 ± 0.34
0.599AsnTrp: 0.599 ± 0.21
2.697AsnTyr: 2.697 ± 0.43
0.0AsnXaa: 0.0 ± 0.0
Pro
2.098ProAla: 2.098 ± 0.639
0.599ProCys: 0.599 ± 0.24
2.922ProAsp: 2.922 ± 0.581
3.596ProGlu: 3.596 ± 0.568
1.349ProPhe: 1.349 ± 0.328
1.723ProGly: 1.723 ± 0.248
0.674ProHis: 0.674 ± 0.22
3.671ProIle: 3.671 ± 0.544
3.671ProLys: 3.671 ± 0.942
2.023ProLeu: 2.023 ± 0.443
0.974ProMet: 0.974 ± 0.273
1.349ProAsn: 1.349 ± 0.362
0.524ProPro: 0.524 ± 0.23
1.798ProGln: 1.798 ± 0.426
1.199ProArg: 1.199 ± 0.282
2.622ProSer: 2.622 ± 0.448
2.398ProThr: 2.398 ± 0.526
1.873ProVal: 1.873 ± 0.513
0.45ProTrp: 0.45 ± 0.21
1.498ProTyr: 1.498 ± 0.363
0.0ProXaa: 0.0 ± 0.0
Gln
2.398GlnAla: 2.398 ± 0.441
0.225GlnCys: 0.225 ± 0.129
1.573GlnAsp: 1.573 ± 0.313
2.023GlnGlu: 2.023 ± 0.314
1.049GlnPhe: 1.049 ± 0.317
1.723GlnGly: 1.723 ± 0.336
0.225GlnHis: 0.225 ± 0.147
3.446GlnIle: 3.446 ± 0.554
3.821GlnLys: 3.821 ± 0.721
2.622GlnLeu: 2.622 ± 0.444
1.199GlnMet: 1.199 ± 0.326
2.697GlnAsn: 2.697 ± 0.478
1.424GlnPro: 1.424 ± 0.383
1.798GlnGln: 1.798 ± 0.461
2.023GlnArg: 2.023 ± 0.445
2.248GlnSer: 2.248 ± 0.394
2.173GlnThr: 2.173 ± 0.402
1.873GlnVal: 1.873 ± 0.397
0.375GlnTrp: 0.375 ± 0.187
1.723GlnTyr: 1.723 ± 0.389
0.0GlnXaa: 0.0 ± 0.0
Arg
2.398ArgAla: 2.398 ± 0.451
0.15ArgCys: 0.15 ± 0.117
2.547ArgAsp: 2.547 ± 0.388
3.297ArgGlu: 3.297 ± 0.653
2.098ArgPhe: 2.098 ± 0.396
1.424ArgGly: 1.424 ± 0.302
1.049ArgHis: 1.049 ± 0.296
2.847ArgIle: 2.847 ± 0.44
3.446ArgLys: 3.446 ± 0.589
3.971ArgLeu: 3.971 ± 0.522
1.349ArgMet: 1.349 ± 0.274
2.248ArgAsn: 2.248 ± 0.44
1.199ArgPro: 1.199 ± 0.211
0.974ArgGln: 0.974 ± 0.232
1.349ArgArg: 1.349 ± 0.315
2.023ArgSer: 2.023 ± 0.42
2.248ArgThr: 2.248 ± 0.416
2.472ArgVal: 2.472 ± 0.375
0.375ArgTrp: 0.375 ± 0.153
1.274ArgTyr: 1.274 ± 0.3
0.0ArgXaa: 0.0 ± 0.0
Ser
3.821SerAla: 3.821 ± 0.595
0.3SerCys: 0.3 ± 0.15
3.821SerAsp: 3.821 ± 0.361
5.02SerGlu: 5.02 ± 0.513
3.896SerPhe: 3.896 ± 0.481
5.095SerGly: 5.095 ± 0.528
0.674SerHis: 0.674 ± 0.174
3.896SerIle: 3.896 ± 0.655
4.121SerLys: 4.121 ± 0.682
5.02SerLeu: 5.02 ± 0.625
1.124SerMet: 1.124 ± 0.244
3.222SerAsn: 3.222 ± 0.613
1.349SerPro: 1.349 ± 0.293
2.023SerGln: 2.023 ± 0.338
2.098SerArg: 2.098 ± 0.352
2.847SerSer: 2.847 ± 0.449
3.971SerThr: 3.971 ± 0.619
3.671SerVal: 3.671 ± 0.483
0.974SerTrp: 0.974 ± 0.308
2.173SerTyr: 2.173 ± 0.452
0.0SerXaa: 0.0 ± 0.0
Thr
4.645ThrAla: 4.645 ± 0.679
0.45ThrCys: 0.45 ± 0.173
4.87ThrAsp: 4.87 ± 0.684
4.271ThrGlu: 4.271 ± 0.437
2.622ThrPhe: 2.622 ± 0.393
5.17ThrGly: 5.17 ± 0.657
0.824ThrHis: 0.824 ± 0.23
4.271ThrIle: 4.271 ± 0.536
4.645ThrLys: 4.645 ± 0.538
5.02ThrLeu: 5.02 ± 0.612
0.749ThrMet: 0.749 ± 0.237
2.997ThrAsn: 2.997 ± 0.599
4.945ThrPro: 4.945 ± 1.069
1.873ThrGln: 1.873 ± 0.503
2.098ThrArg: 2.098 ± 0.359
3.446ThrSer: 3.446 ± 0.558
3.671ThrThr: 3.671 ± 0.526
4.645ThrVal: 4.645 ± 0.604
0.45ThrTrp: 0.45 ± 0.149
2.323ThrTyr: 2.323 ± 0.553
0.0ThrXaa: 0.0 ± 0.0
Val
3.521ValAla: 3.521 ± 0.526
0.749ValCys: 0.749 ± 0.187
4.645ValAsp: 4.645 ± 0.546
3.821ValGlu: 3.821 ± 0.602
3.521ValPhe: 3.521 ± 0.629
3.372ValGly: 3.372 ± 0.587
0.824ValHis: 0.824 ± 0.239
3.971ValIle: 3.971 ± 0.572
5.095ValLys: 5.095 ± 0.723
4.795ValLeu: 4.795 ± 0.582
0.899ValMet: 0.899 ± 0.251
3.147ValAsn: 3.147 ± 0.546
2.772ValPro: 2.772 ± 0.418
2.997ValGln: 2.997 ± 0.325
2.697ValArg: 2.697 ± 0.368
4.645ValSer: 4.645 ± 0.57
3.372ValThr: 3.372 ± 0.52
2.772ValVal: 2.772 ± 0.517
0.524ValTrp: 0.524 ± 0.27
1.948ValTyr: 1.948 ± 0.333
0.0ValXaa: 0.0 ± 0.0
Trp
0.524TrpAla: 0.524 ± 0.174
0.225TrpCys: 0.225 ± 0.133
1.199TrpAsp: 1.199 ± 0.333
0.974TrpGlu: 0.974 ± 0.235
0.824TrpPhe: 0.824 ± 0.197
0.899TrpGly: 0.899 ± 0.194
0.225TrpHis: 0.225 ± 0.106
0.599TrpIle: 0.599 ± 0.211
0.749TrpLys: 0.749 ± 0.246
1.124TrpLeu: 1.124 ± 0.277
0.225TrpMet: 0.225 ± 0.12
0.599TrpAsn: 0.599 ± 0.253
0.375TrpPro: 0.375 ± 0.175
0.524TrpGln: 0.524 ± 0.208
0.824TrpArg: 0.824 ± 0.197
1.349TrpSer: 1.349 ± 0.256
0.974TrpThr: 0.974 ± 0.27
1.124TrpVal: 1.124 ± 0.28
0.225TrpTrp: 0.225 ± 0.149
0.599TrpTyr: 0.599 ± 0.203
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.798TyrAla: 1.798 ± 0.339
0.749TyrCys: 0.749 ± 0.268
3.147TyrAsp: 3.147 ± 0.393
2.173TyrGlu: 2.173 ± 0.443
1.648TyrPhe: 1.648 ± 0.37
2.547TyrGly: 2.547 ± 0.463
0.524TyrHis: 0.524 ± 0.21
2.997TyrIle: 2.997 ± 0.527
3.821TyrLys: 3.821 ± 0.426
4.196TyrLeu: 4.196 ± 0.593
0.974TyrMet: 0.974 ± 0.27
2.023TyrAsn: 2.023 ± 0.403
1.648TyrPro: 1.648 ± 0.328
1.424TyrGln: 1.424 ± 0.265
1.723TyrArg: 1.723 ± 0.417
3.147TyrSer: 3.147 ± 0.448
2.023TyrThr: 2.023 ± 0.377
1.573TyrVal: 1.573 ± 0.347
0.749TyrTrp: 0.749 ± 0.251
1.274TyrTyr: 1.274 ± 0.305
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (13348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski