Capybara microvirus Cap3_SP_383
Average proteome isoelectric point is 5.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5R6|A0A4P8W5R6_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_383 OX=2585439 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.56 FNGFNGTDD10 pKa = 3.82 IIQVPTPKK18 pKa = 10.13 NPPMMLLQPNEE29 pKa = 4.14 FGEE32 pKa = 4.38 MEE34 pKa = 4.66 LVAFDD39 pKa = 3.82 DD40 pKa = 4.3 TADD43 pKa = 3.55 LGYY46 pKa = 11.12 ASDD49 pKa = 4.07 YY50 pKa = 11.28 DD51 pKa = 3.58 ITTLLEE57 pKa = 3.99 NGIDD61 pKa = 3.56 PRR63 pKa = 11.84 FVGNTKK69 pKa = 8.97 PTGSMLDD76 pKa = 3.26 AYY78 pKa = 10.84 SGLEE82 pKa = 3.83 AFQPATNINEE92 pKa = 4.25 TNEE95 pKa = 3.86
Molecular weight: 10.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.193
IPC2_protein 3.745
IPC_protein 3.668
Toseland 3.478
ProMoST 3.808
Dawson 3.668
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.503
Grimsley 3.389
Solomon 3.63
Lehninger 3.592
Nozaki 3.795
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.617
Sillero 3.795
Patrickios 3.35
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A4P8W5Z5|A0A4P8W5Z5_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_383 OX=2585439 PE=3 SV=1
MM1 pKa = 8.21 DD2 pKa = 5.38 IFPKK6 pKa = 10.27 SDD8 pKa = 2.56 NHH10 pKa = 7.58 ALIKK14 pKa = 10.41 KK15 pKa = 9.65 GYY17 pKa = 7.27 TPNRR21 pKa = 11.84 YY22 pKa = 9.7 AMQIMNKK29 pKa = 7.25 THH31 pKa = 6.27 YY32 pKa = 9.43 LHH34 pKa = 7.18 PDD36 pKa = 2.33 MRR38 pKa = 11.84 YY39 pKa = 9.1 RR40 pKa = 11.84 YY41 pKa = 9.76 NVINPYY47 pKa = 9.38 SFCRR51 pKa = 11.84 MFSQQQSYY59 pKa = 9.7 YY60 pKa = 9.89 IRR62 pKa = 11.84 AYY64 pKa = 8.6 YY65 pKa = 8.89 QWLVTNEE72 pKa = 4.03 QLGKK76 pKa = 9.28 TIFLTFNYY84 pKa = 10.03 NNNALPHH91 pKa = 6.08 NYY93 pKa = 10.34 GYY95 pKa = 7.84 VTCDD99 pKa = 2.86 NSHH102 pKa = 5.09 IHH104 pKa = 6.52 ALFRR108 pKa = 11.84 NSYY111 pKa = 8.02 LHH113 pKa = 7.05 KK114 pKa = 10.37 KK115 pKa = 9.16 FKK117 pKa = 10.3 DD118 pKa = 3.53 YY119 pKa = 10.49 RR120 pKa = 11.84 IHH122 pKa = 7.36 YY123 pKa = 8.34 IICTEE128 pKa = 3.98 FGEE131 pKa = 4.84 GAGIRR136 pKa = 11.84 KK137 pKa = 9.74 KK138 pKa = 11.05 GEE140 pKa = 3.8 NPHH143 pKa = 5.06 YY144 pKa = 10.28 HH145 pKa = 6.11 ALIYY149 pKa = 8.67 LTHH152 pKa = 7.17 RR153 pKa = 11.84 NGEE156 pKa = 4.36 CVTNHH161 pKa = 5.54 VCRR164 pKa = 11.84 QFLAEE169 pKa = 5.59 CRR171 pKa = 11.84 RR172 pKa = 11.84 FWQGNPNPASKK183 pKa = 9.88 TNDD186 pKa = 2.74 PRR188 pKa = 11.84 NFIFGKK194 pKa = 10.4 VEE196 pKa = 3.81 VAKK199 pKa = 11.04 GKK201 pKa = 10.63 GLFVDD206 pKa = 4.9 SFHH209 pKa = 8.24 SMQYY213 pKa = 8.88 VSKK216 pKa = 9.55 YY217 pKa = 8.01 TIKK220 pKa = 11.04 DD221 pKa = 2.82 ADD223 pKa = 3.54 TRR225 pKa = 11.84 SRR227 pKa = 11.84 MKK229 pKa = 10.48 KK230 pKa = 9.75 IEE232 pKa = 3.91 DD233 pKa = 4.12 AIYY236 pKa = 10.28 NEE238 pKa = 3.24 IRR240 pKa = 11.84 TTVFRR245 pKa = 11.84 DD246 pKa = 3.23 EE247 pKa = 4.76 SVNHH251 pKa = 5.65 TLRR254 pKa = 11.84 DD255 pKa = 3.56 VLEE258 pKa = 3.99 FHH260 pKa = 7.03 PFVDD264 pKa = 4.75 GVNDD268 pKa = 3.54 EE269 pKa = 4.29 QFNLSLPAHH278 pKa = 5.39 YY279 pKa = 9.94 FGYY282 pKa = 10.67 EE283 pKa = 3.8 LLHH286 pKa = 6.37 YY287 pKa = 9.12 YY288 pKa = 10.27 QGVDD292 pKa = 2.74 TGVRR296 pKa = 11.84 GEE298 pKa = 4.18 FKK300 pKa = 10.79 SYY302 pKa = 9.3 LTLLEE307 pKa = 4.27 EE308 pKa = 4.2 QVKK311 pKa = 10.03 QLFEE315 pKa = 4.04 EE316 pKa = 4.51 RR317 pKa = 11.84 LQEE320 pKa = 3.84 EE321 pKa = 4.82 KK322 pKa = 10.81 KK323 pKa = 10.8 RR324 pKa = 11.84 IFPKK328 pKa = 10.44 VFMSKK333 pKa = 10.36 GFGSHH338 pKa = 6.16 ALEE341 pKa = 5.82 HH342 pKa = 6.18 ITPDD346 pKa = 3.36 YY347 pKa = 10.02 KK348 pKa = 10.81 IPISTPKK355 pKa = 10.32 GIKK358 pKa = 9.45 NVPLPMYY365 pKa = 10.29 LYY367 pKa = 10.53 RR368 pKa = 11.84 KK369 pKa = 9.83 LFYY372 pKa = 10.82 DD373 pKa = 3.53 VVKK376 pKa = 8.52 TQEE379 pKa = 4.32 GQVHH383 pKa = 4.74 YY384 pKa = 9.93 HH385 pKa = 5.26 LNEE388 pKa = 4.05 RR389 pKa = 11.84 GVQYY393 pKa = 10.78 KK394 pKa = 9.69 IDD396 pKa = 3.81 KK397 pKa = 9.94 FDD399 pKa = 3.8 NDD401 pKa = 3.96 IYY403 pKa = 11.35 NLRR406 pKa = 11.84 QDD408 pKa = 3.4 IYY410 pKa = 11.68 AFVQNYY416 pKa = 10.0 DD417 pKa = 4.3 DD418 pKa = 4.41 LRR420 pKa = 11.84 EE421 pKa = 3.94 MCDD424 pKa = 2.93 IYY426 pKa = 11.21 NKK428 pKa = 10.37 NMYY431 pKa = 10.05 DD432 pKa = 3.18 SRR434 pKa = 11.84 FINIYY439 pKa = 9.52 HH440 pKa = 7.17 AYY442 pKa = 10.02 AVYY445 pKa = 10.15 KK446 pKa = 9.86 YY447 pKa = 9.53 IYY449 pKa = 8.19 EE450 pKa = 3.85 GRR452 pKa = 11.84 MFSLINPPQLDD463 pKa = 3.85 FYY465 pKa = 11.2 EE466 pKa = 4.33 DD467 pKa = 3.69 FKK469 pKa = 11.55 RR470 pKa = 11.84 FVLPHH475 pKa = 7.35 DD476 pKa = 5.48 DD477 pKa = 3.24 EE478 pKa = 6.2 DD479 pKa = 5.91 LYY481 pKa = 12.05 VNTLDD486 pKa = 4.75 VDD488 pKa = 3.78 EE489 pKa = 5.53 AKK491 pKa = 10.75 QNLIRR496 pKa = 11.84 RR497 pKa = 11.84 MRR499 pKa = 11.84 LCDD502 pKa = 3.52 YY503 pKa = 10.49 AYY505 pKa = 10.57 HH506 pKa = 7.21 PDD508 pKa = 3.32 FRR510 pKa = 11.84 HH511 pKa = 5.08 YY512 pKa = 10.65 RR513 pKa = 11.84 SLFEE517 pKa = 4.52 TISDD521 pKa = 4.59 RR522 pKa = 11.84 IDD524 pKa = 3.4 AYY526 pKa = 11.49 NFDD529 pKa = 4.25 KK530 pKa = 11.05 DD531 pKa = 3.6 AEE533 pKa = 4.25 RR534 pKa = 11.84 MKK536 pKa = 10.95 SYY538 pKa = 10.74 IEE540 pKa = 4.07 RR541 pKa = 11.84 KK542 pKa = 10.3 NITTKK547 pKa = 10.44 HH548 pKa = 5.84 KK549 pKa = 9.6 KK550 pKa = 7.56 TLL552 pKa = 3.53
Molecular weight: 66.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.815
IPC2_protein 8.024
IPC_protein 7.878
Toseland 7.936
ProMoST 8.346
Dawson 8.595
Bjellqvist 8.712
Wikipedia 8.668
Rodwell 8.639
Grimsley 8.097
Solomon 8.668
Lehninger 8.639
Nozaki 8.682
DTASelect 8.565
Thurlkill 8.595
EMBOSS 8.741
Sillero 8.814
Patrickios 3.834
IPC_peptide 8.653
IPC2_peptide 7.571
IPC2.peptide.svr19 7.759
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1629
95
602
407.3
46.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.262 ± 1.376
0.798 ± 0.276
7.612 ± 0.626
4.911 ± 1.162
5.279 ± 0.86
5.893 ± 0.795
2.21 ± 1.107
5.402 ± 0.493
6.016 ± 0.687
8.165 ± 0.619
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.762 ± 0.407
7.428 ± 1.315
3.376 ± 1.055
4.604 ± 1.054
5.034 ± 0.81
5.955 ± 1.114
6.446 ± 0.996
4.85 ± 0.587
0.859 ± 0.292
6.139 ± 1.493
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here