Pararobbsia alpina

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Pararobbsia

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S7B4P0|A0A6S7B4P0_9BURK Rieske domain-containing protein OS=Pararobbsia alpina OX=621374 GN=LMG28138_02334 PE=4 SV=1
MM1 pKa = 6.68KK2 pKa = 10.06TFWVWLLSAVLLGSVAACGGGHH24 pKa = 7.47DD25 pKa = 4.45DD26 pKa = 5.43HH27 pKa = 6.66STQRR31 pKa = 11.84PFGVAGIMEE40 pKa = 5.33GIWNGNLHH48 pKa = 6.59SNLNGGDD55 pKa = 2.79TGMFVIVTADD65 pKa = 3.36GALSLITNDD74 pKa = 3.8CQQISANIAANGPFFSGAGTTYY96 pKa = 10.87LQTNCDD102 pKa = 3.65GTDD105 pKa = 3.3VTLVSPTASAGPVQPFQIVGQFDD128 pKa = 4.29DD129 pKa = 3.69RR130 pKa = 11.84TGTAFANYY138 pKa = 6.82TTADD142 pKa = 3.59DD143 pKa = 3.89SGTISFLNFYY153 pKa = 9.71PDD155 pKa = 3.52YY156 pKa = 10.52FNEE159 pKa = 4.7PGTLPRR165 pKa = 11.84AAGAYY170 pKa = 10.31SFTQTLTALSIDD182 pKa = 3.64VNGILSYY189 pKa = 11.02RR190 pKa = 11.84DD191 pKa = 3.14AAGQAFPGTLFVIDD205 pKa = 4.39PTVDD209 pKa = 3.02AYY211 pKa = 10.96GMTLQVGSQTLTGLATLVDD230 pKa = 4.65DD231 pKa = 5.72GSGRR235 pKa = 11.84DD236 pKa = 3.45NDD238 pKa = 3.87FLFAVADD245 pKa = 4.05GNLAYY250 pKa = 10.57SAVLKK255 pKa = 10.66RR256 pKa = 11.84DD257 pKa = 3.15

Molecular weight: 26.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S7CUD4|A0A6S7CUD4_9BURK Lipopolysaccharide export system protein LptA OS=Pararobbsia alpina OX=621374 GN=lptA PE=3 SV=1
MM1 pKa = 7.48RR2 pKa = 11.84AAHH5 pKa = 6.59RR6 pKa = 11.84FARR9 pKa = 11.84VHH11 pKa = 6.09RR12 pKa = 11.84ASPCRR17 pKa = 11.84TDD19 pKa = 3.24RR20 pKa = 11.84RR21 pKa = 11.84QAMRR25 pKa = 11.84RR26 pKa = 11.84APSVRR31 pKa = 11.84TLARR35 pKa = 11.84AAHH38 pKa = 6.48ANRR41 pKa = 11.84GPMAMRR47 pKa = 11.84HH48 pKa = 4.28VHH50 pKa = 6.46LALTSTHH57 pKa = 6.3RR58 pKa = 11.84ASRR61 pKa = 11.84APMVTRR67 pKa = 11.84HH68 pKa = 5.04VHH70 pKa = 5.38PVPTSMVRR78 pKa = 11.84ASRR81 pKa = 11.84APMVTRR87 pKa = 11.84HH88 pKa = 5.04VHH90 pKa = 5.38PVPTSMVRR98 pKa = 11.84ASRR101 pKa = 11.84APMVTRR107 pKa = 11.84HH108 pKa = 5.85VYY110 pKa = 9.21PVPTSMVRR118 pKa = 11.84ASRR121 pKa = 11.84APMVTRR127 pKa = 11.84RR128 pKa = 11.84VHH130 pKa = 5.9PVPTSMARR138 pKa = 11.84ASRR141 pKa = 11.84APMVTRR147 pKa = 11.84RR148 pKa = 11.84AHH150 pKa = 5.79PVPTSMARR158 pKa = 11.84ASRR161 pKa = 11.84APMVTPRR168 pKa = 11.84VHH170 pKa = 6.73PVPTSMARR178 pKa = 11.84ASRR181 pKa = 11.84APMVTRR187 pKa = 11.84HH188 pKa = 5.39VRR190 pKa = 11.84LAPTSMVRR198 pKa = 11.84VSRR201 pKa = 11.84VPMVMSLVHH210 pKa = 6.39LVAISIPRR218 pKa = 11.84AGRR221 pKa = 11.84VRR223 pKa = 11.84VAMRR227 pKa = 11.84GVPQAMRR234 pKa = 11.84HH235 pKa = 4.86LDD237 pKa = 3.33ARR239 pKa = 4.21

Molecular weight: 26.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6080

0

6080

1865431

29

2929

306.8

33.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.191 ± 0.038

0.927 ± 0.009

5.448 ± 0.022

5.235 ± 0.03

3.668 ± 0.02

8.186 ± 0.029

2.353 ± 0.016

4.891 ± 0.023

3.106 ± 0.028

10.264 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.014

2.824 ± 0.021

5.085 ± 0.024

3.594 ± 0.022

6.959 ± 0.031

6.034 ± 0.024

5.481 ± 0.026

7.666 ± 0.026

1.344 ± 0.012

2.365 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski