Porphyromonas sp. COT-290 OH860

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas; unclassified Porphyromonas

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1618 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2F9K8|A0A0A2F9K8_9PORP Cell shape protein MreC OS=Porphyromonas sp. COT-290 OH860 OX=1515615 GN=HQ41_03445 PE=3 SV=1
MM1 pKa = 7.44SNQYY5 pKa = 11.19LEE7 pKa = 4.22MEE9 pKa = 4.24EE10 pKa = 4.49QIVAMLRR17 pKa = 11.84TVYY20 pKa = 10.7DD21 pKa = 3.19PEE23 pKa = 5.14IPVNVYY29 pKa = 10.84DD30 pKa = 5.74LGLIYY35 pKa = 10.92NVDD38 pKa = 3.12VDD40 pKa = 4.28DD41 pKa = 4.82NKK43 pKa = 10.61FVTITMTLTAPNCPAADD60 pKa = 4.75FIVEE64 pKa = 4.09DD65 pKa = 3.71VKK67 pKa = 11.15MKK69 pKa = 10.28TEE71 pKa = 4.06YY72 pKa = 10.83VDD74 pKa = 3.8GVTGVAVEE82 pKa = 4.22LTFEE86 pKa = 4.41PEE88 pKa = 3.64WNKK91 pKa = 11.36DD92 pKa = 3.55MMSEE96 pKa = 4.02EE97 pKa = 4.37ALLEE101 pKa = 4.43LGFLL105 pKa = 4.08

Molecular weight: 11.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2FBN2|A0A0A2FBN2_9PORP Lipoyl synthase OS=Porphyromonas sp. COT-290 OH860 OX=1515615 GN=lipA PE=3 SV=1
MM1 pKa = 7.06ATKK4 pKa = 8.82PTEE7 pKa = 4.06PAPSFLQVYY16 pKa = 5.99TQVRR20 pKa = 11.84RR21 pKa = 11.84DD22 pKa = 3.52RR23 pKa = 11.84MKK25 pKa = 10.7HH26 pKa = 4.47SFLRR30 pKa = 11.84QINACVDD37 pKa = 2.77WRR39 pKa = 11.84GIRR42 pKa = 11.84TT43 pKa = 3.52

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1618

0

1618

605102

26

2460

374.0

41.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.129 ± 0.053

0.959 ± 0.017

5.262 ± 0.035

6.754 ± 0.052

3.846 ± 0.037

7.222 ± 0.049

2.171 ± 0.028

6.407 ± 0.043

5.261 ± 0.053

10.377 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.027

3.73 ± 0.046

4.107 ± 0.03

3.913 ± 0.033

5.939 ± 0.059

6.502 ± 0.039

5.234 ± 0.03

6.354 ± 0.047

1.178 ± 0.022

4.122 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski