Alloactinosynnema sp. L-07

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Alloactinosynnema; unclassified Alloactinosynnema

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H5CJT3|A0A0H5CJT3_9PSEU Uncharacterized protein OS=Alloactinosynnema sp. L-07 OX=1653480 PE=4 SV=1
MM1 pKa = 7.36SAATVWRR8 pKa = 11.84ASALAALVVVPSLVLTGTAQAQDD31 pKa = 3.36GTVQVISGEE40 pKa = 4.15LTIKK44 pKa = 10.63AAGRR48 pKa = 11.84QVNKK52 pKa = 10.24VLVSHH57 pKa = 6.48VGDD60 pKa = 3.6MVVVSDD66 pKa = 5.02VVPLSAGPGCRR77 pKa = 11.84KK78 pKa = 9.86YY79 pKa = 10.86EE80 pKa = 4.06RR81 pKa = 11.84TEE83 pKa = 3.92VRR85 pKa = 11.84CPATGVTSLAIDD97 pKa = 4.51LADD100 pKa = 4.4LDD102 pKa = 5.2DD103 pKa = 4.06RR104 pKa = 11.84AAYY107 pKa = 8.67KK108 pKa = 10.98GPMPVYY114 pKa = 10.64ADD116 pKa = 3.93GGAGDD121 pKa = 5.49DD122 pKa = 4.17IVTGGYY128 pKa = 10.5GGDD131 pKa = 3.46KK132 pKa = 10.69LLGGEE137 pKa = 4.24GVDD140 pKa = 3.98YY141 pKa = 11.42VSGGPGNDD149 pKa = 3.4TLIGGPGADD158 pKa = 2.79HH159 pKa = 7.16VYY161 pKa = 10.9GGTHH165 pKa = 6.64NDD167 pKa = 2.95VLYY170 pKa = 11.22GDD172 pKa = 5.18LPYY175 pKa = 10.13ATPLCDD181 pKa = 5.07SGLPSCGDD189 pKa = 3.33FLYY192 pKa = 11.04GGDD195 pKa = 4.36GADD198 pKa = 3.92KK199 pKa = 10.46IYY201 pKa = 10.78GGEE204 pKa = 4.04WADD207 pKa = 3.71TLAGEE212 pKa = 4.99GGNDD216 pKa = 3.78LLRR219 pKa = 11.84DD220 pKa = 4.22FSGGATFRR228 pKa = 11.84GGPGDD233 pKa = 3.59DD234 pKa = 3.53TMIGASPSMRR244 pKa = 11.84DD245 pKa = 3.16TVDD248 pKa = 3.44YY249 pKa = 10.93SDD251 pKa = 4.67HH252 pKa = 5.85LTDD255 pKa = 4.07VKK257 pKa = 11.33VDD259 pKa = 3.69LTAGTGGDD267 pKa = 3.8VQAGEE272 pKa = 4.81HH273 pKa = 6.03DD274 pKa = 4.94TITSIQDD281 pKa = 3.28VNGGAGNDD289 pKa = 3.67SLKK292 pKa = 11.19GNSGDD297 pKa = 4.53NNLTGNGGNDD307 pKa = 3.35SADD310 pKa = 3.6GGDD313 pKa = 3.31SVLYY317 pKa = 10.79NDD319 pKa = 3.71ICNVEE324 pKa = 4.29SPSYY328 pKa = 10.64CC329 pKa = 3.71

Molecular weight: 33.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H5CDQ8|A0A0H5CDQ8_9PSEU Uncharacterized protein OS=Alloactinosynnema sp. L-07 OX=1653480 PE=4 SV=1
MM1 pKa = 6.94GQGRR5 pKa = 11.84SFRR8 pKa = 11.84APLLAGRR15 pKa = 11.84GPLARR20 pKa = 11.84IHH22 pKa = 6.27PLVVFLLVAGLFAAGVLVRR41 pKa = 11.84GVLGAALLGVLAAGVAVLLAATWRR65 pKa = 11.84VQTASQRR72 pKa = 11.84VGRR75 pKa = 11.84VLVLGLLVAIAVSVLL90 pKa = 3.26

Molecular weight: 9.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7267

0

7267

2223605

37

5032

306.0

32.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.339 ± 0.045

0.816 ± 0.01

6.423 ± 0.022

5.174 ± 0.028

2.821 ± 0.018

9.008 ± 0.029

2.259 ± 0.015

3.697 ± 0.021

2.194 ± 0.022

10.091 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.806 ± 0.013

2.0 ± 0.022

5.905 ± 0.026

2.723 ± 0.017

7.769 ± 0.035

5.14 ± 0.024

6.435 ± 0.038

8.931 ± 0.031

1.527 ± 0.012

1.943 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski