Rhizobium yanglingense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4086 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A245ZCC3|A0A245ZCC3_9RHIZ DNA-binding protein OS=Rhizobium yanglingense OX=61013 GN=AJ87_05220 PE=4 SV=1
MM1 pKa = 6.93YY2 pKa = 9.71RR3 pKa = 11.84ALTRR7 pKa = 11.84DD8 pKa = 2.97IEE10 pKa = 4.73VVVEE14 pKa = 3.87PFYY17 pKa = 11.31LEE19 pKa = 4.21EE20 pKa = 4.77QSDD23 pKa = 4.37PDD25 pKa = 3.5DD26 pKa = 4.65DD27 pKa = 5.04RR28 pKa = 11.84YY29 pKa = 9.9VWGYY33 pKa = 10.79RR34 pKa = 11.84IVISNNSDD42 pKa = 3.17LTVRR46 pKa = 11.84LINRR50 pKa = 11.84YY51 pKa = 7.93WNITDD56 pKa = 3.39QNGLVDD62 pKa = 4.31EE63 pKa = 4.56VTGPGVVGEE72 pKa = 4.28QPRR75 pKa = 11.84LEE77 pKa = 4.64PGDD80 pKa = 3.63TYY82 pKa = 10.78EE83 pKa = 4.33YY84 pKa = 11.37SSGCPLDD91 pKa = 3.95TPSGLMFGHH100 pKa = 5.92YY101 pKa = 9.54QMEE104 pKa = 4.56TEE106 pKa = 4.57DD107 pKa = 5.06GEE109 pKa = 4.6LFDD112 pKa = 4.98VEE114 pKa = 4.82IPAFSLDD121 pKa = 3.5SPGLLRR127 pKa = 11.84VLNN130 pKa = 4.19

Molecular weight: 14.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A245Z9U5|A0A245Z9U5_9RHIZ Uncharacterized protein OS=Rhizobium yanglingense OX=61013 GN=AJ87_08375 PE=4 SV=1
MM1 pKa = 6.77PQNWGHH7 pKa = 5.19GQRR10 pKa = 11.84GLRR13 pKa = 11.84LNHH16 pKa = 6.31HH17 pKa = 6.09GQRR20 pKa = 11.84PVVGLALHH28 pKa = 6.71MPPSMRR34 pKa = 11.84INSQKK39 pKa = 10.04VWVLVASAILRR50 pKa = 11.84RR51 pKa = 11.84PRR53 pKa = 11.84LIPSARR59 pKa = 11.84MTFISPIRR67 pKa = 3.77

Molecular weight: 7.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4086

0

4086

623830

36

656

152.7

16.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.367 ± 0.062

1.297 ± 0.018

5.501 ± 0.035

5.713 ± 0.041

3.822 ± 0.029

7.586 ± 0.04

2.213 ± 0.022

5.371 ± 0.029

3.824 ± 0.038

9.438 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.018

2.791 ± 0.024

5.14 ± 0.03

3.323 ± 0.03

8.236 ± 0.054

6.48 ± 0.038

5.111 ± 0.036

6.745 ± 0.038

1.33 ± 0.02

2.068 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski