Pelagibacter phage HTVC201P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pelagivirus; unclassified Pelagivirus

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NU81|A0A4Y1NU81_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC201P OX=2283024 GN=P201_gp50 PE=4 SV=1
MM1 pKa = 7.47ANSFVRR7 pKa = 11.84YY8 pKa = 7.53TGNNSTTSYY17 pKa = 10.59SIPFSYY23 pKa = 10.5RR24 pKa = 11.84ATSDD28 pKa = 3.24LTVTLSGVVTTAYY41 pKa = 9.21TLNSAGTTLTFNTAPAQDD59 pKa = 3.47VAIEE63 pKa = 3.8IRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84TSQGTKK73 pKa = 10.07LVDD76 pKa = 3.56YY77 pKa = 10.83ASGSVLTEE85 pKa = 3.68NDD87 pKa = 3.94LDD89 pKa = 4.06TDD91 pKa = 3.63SDD93 pKa = 3.8QAFFMSQEE101 pKa = 4.56AIDD104 pKa = 4.32DD105 pKa = 4.22AGDD108 pKa = 3.66VIKK111 pKa = 11.09VSNTNFQWDD120 pKa = 3.95VQNKK124 pKa = 9.66RR125 pKa = 11.84LTNVANPVNDD135 pKa = 3.36TDD137 pKa = 4.1AVNKK141 pKa = 9.93QFISTNLPNITTVAGISADD160 pKa = 3.48VTTVAGISSNVTTVANDD177 pKa = 3.35TTNIGTVATNMPSITTVATNINDD200 pKa = 3.93VIKK203 pKa = 11.06VADD206 pKa = 4.73DD207 pKa = 3.71LNEE210 pKa = 4.8AISEE214 pKa = 4.4VEE216 pKa = 4.24TVANDD221 pKa = 3.58LNEE224 pKa = 4.22ATSEE228 pKa = 4.16IEE230 pKa = 4.1VVANNIVNVNTVGTINADD248 pKa = 3.27VTTVANNEE256 pKa = 3.83TDD258 pKa = 3.26IQTLADD264 pKa = 4.24LEE266 pKa = 5.04DD267 pKa = 4.01GTVTTNGLSTLAGLNTEE284 pKa = 3.89IQGVYY289 pKa = 10.52NIRR292 pKa = 11.84TNVTNVDD299 pKa = 3.69TNSANVNLVAGQISPTNNVSAVSAVATEE327 pKa = 3.92IGTLGALGTEE337 pKa = 3.91ITNLNNIRR345 pKa = 11.84TDD347 pKa = 2.9ITGVNNIAPAVTAVNNNSANINTVAGLDD375 pKa = 3.74TEE377 pKa = 4.45ITALGASGTVASINTVASNLASVNNFGEE405 pKa = 4.61VYY407 pKa = 10.0RR408 pKa = 11.84ISATAPTTSLDD419 pKa = 3.8NGDD422 pKa = 3.17MWFDD426 pKa = 3.44TTAGKK431 pKa = 10.42LKK433 pKa = 9.75IWNGSSFDD441 pKa = 4.06LAGSSINGTSARR453 pKa = 11.84FKK455 pKa = 9.34YY456 pKa = 8.74TATASQTTFTGADD469 pKa = 3.49DD470 pKa = 4.13NGNTLAYY477 pKa = 10.26DD478 pKa = 3.44SGFTDD483 pKa = 3.98VYY485 pKa = 11.02LNGVKK490 pKa = 10.07LVNGSDD496 pKa = 3.53YY497 pKa = 10.98TATDD501 pKa = 3.61GSNVVLTTGASVNDD515 pKa = 3.34ILEE518 pKa = 4.12IVGFGTFSVADD529 pKa = 4.37FDD531 pKa = 5.44ASGLTGTINIARR543 pKa = 11.84LADD546 pKa = 3.58NSVTNAKK553 pKa = 9.64LANQSITINGSAVNLGGSVTVGEE576 pKa = 4.52TKK578 pKa = 9.18PTISSISPSTITNAQTSITITGSNFTSVPQVEE610 pKa = 4.76FLNPSTGIWYY620 pKa = 9.82VADD623 pKa = 3.22TVTYY627 pKa = 10.71NNSTSLTVNATLGVDD642 pKa = 3.59GTYY645 pKa = 10.3KK646 pKa = 10.15IRR648 pKa = 11.84IEE650 pKa = 4.26NPDD653 pKa = 3.26GNAVISSTNILTVSDD668 pKa = 3.76APTWTTASGTLGTFAGDD685 pKa = 3.63FSGTLATVVATSDD698 pKa = 3.47SAVTFSEE705 pKa = 4.29VGSNLATANVTLSSGGVLSTTDD727 pKa = 3.79FGGSSTTATTYY738 pKa = 11.13NFTIRR743 pKa = 11.84ATDD746 pKa = 3.77AEE748 pKa = 4.44NQTADD753 pKa = 3.8RR754 pKa = 11.84NFSLTSSFGATGGGQFNN771 pKa = 4.04

Molecular weight: 79.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NTQ4|A0A4Y1NTQ4_9CAUD Lysozyme OS=Pelagibacter phage HTVC201P OX=2283024 GN=P201_gp52 PE=4 SV=1
MM1 pKa = 7.74IIYY4 pKa = 9.65GYY6 pKa = 8.41SVKK9 pKa = 8.81TWRR12 pKa = 11.84DD13 pKa = 2.62KK14 pKa = 11.49AIIYY18 pKa = 7.47WRR20 pKa = 11.84NTNKK24 pKa = 10.43KK25 pKa = 10.06LFTLFVLWSIILWAMM40 pKa = 4.03

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12862

35

1247

247.3

27.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.394 ± 0.381

0.879 ± 0.174

5.777 ± 0.199

6.329 ± 0.482

3.965 ± 0.16

6.624 ± 0.324

1.672 ± 0.203

6.655 ± 0.178

8.451 ± 0.716

7.853 ± 0.392

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.223

6.36 ± 0.413

2.939 ± 0.162

3.646 ± 0.257

3.841 ± 0.305

6.912 ± 0.447

7.713 ± 0.756

5.637 ± 0.364

1.291 ± 0.131

3.491 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski