Amino acid dipepetide frequency for Pelagibacter phage HTVC201P

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.877AlaAla: 2.877 ± 0.492
0.389AlaCys: 0.389 ± 0.183
4.976AlaAsp: 4.976 ± 0.557
4.354AlaGlu: 4.354 ± 0.771
2.799AlaPhe: 2.799 ± 0.518
5.21AlaGly: 5.21 ± 0.842
0.622AlaHis: 0.622 ± 0.201
4.976AlaIle: 4.976 ± 0.522
6.92AlaLys: 6.92 ± 0.836
5.676AlaLeu: 5.676 ± 0.597
2.41AlaMet: 2.41 ± 0.37
5.832AlaAsn: 5.832 ± 0.627
2.488AlaPro: 2.488 ± 0.51
2.644AlaGln: 2.644 ± 0.56
2.488AlaArg: 2.488 ± 0.461
5.909AlaSer: 5.909 ± 0.752
5.521AlaThr: 5.521 ± 0.961
4.043AlaVal: 4.043 ± 0.744
1.011AlaTrp: 1.011 ± 0.315
2.799AlaTyr: 2.799 ± 0.408
0.0AlaXaa: 0.0 ± 0.0
Cys
0.389CysAla: 0.389 ± 0.18
0.078CysCys: 0.078 ± 0.078
0.467CysAsp: 0.467 ± 0.202
0.778CysGlu: 0.778 ± 0.256
0.7CysPhe: 0.7 ± 0.186
0.233CysGly: 0.233 ± 0.137
0.233CysHis: 0.233 ± 0.116
0.544CysIle: 0.544 ± 0.317
0.855CysLys: 0.855 ± 0.304
0.7CysLeu: 0.7 ± 0.292
0.622CysMet: 0.622 ± 0.221
0.544CysAsn: 0.544 ± 0.237
0.156CysPro: 0.156 ± 0.108
0.311CysGln: 0.311 ± 0.141
0.467CysArg: 0.467 ± 0.152
0.311CysSer: 0.311 ± 0.159
0.544CysThr: 0.544 ± 0.195
0.389CysVal: 0.389 ± 0.176
0.156CysTrp: 0.156 ± 0.113
0.311CysTyr: 0.311 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
3.965AspAla: 3.965 ± 0.658
0.311AspCys: 0.311 ± 0.147
2.41AspAsp: 2.41 ± 0.423
3.266AspGlu: 3.266 ± 0.517
2.721AspPhe: 2.721 ± 0.357
4.51AspGly: 4.51 ± 0.58
0.311AspHis: 0.311 ± 0.144
4.51AspIle: 4.51 ± 0.388
3.888AspLys: 3.888 ± 0.64
5.443AspLeu: 5.443 ± 0.562
1.711AspMet: 1.711 ± 0.368
3.577AspAsn: 3.577 ± 0.53
1.866AspPro: 1.866 ± 0.337
1.244AspGln: 1.244 ± 0.237
2.877AspArg: 2.877 ± 0.591
3.499AspSer: 3.499 ± 0.523
4.821AspThr: 4.821 ± 0.774
3.343AspVal: 3.343 ± 0.596
0.855AspTrp: 0.855 ± 0.263
2.644AspTyr: 2.644 ± 0.375
0.0AspXaa: 0.0 ± 0.0
Glu
5.054GluAla: 5.054 ± 0.69
0.778GluCys: 0.778 ± 0.288
3.81GluAsp: 3.81 ± 0.587
4.199GluGlu: 4.199 ± 0.673
2.41GluPhe: 2.41 ± 0.512
4.199GluGly: 4.199 ± 0.563
1.711GluHis: 1.711 ± 0.42
5.521GluIle: 5.521 ± 0.596
5.832GluLys: 5.832 ± 1.066
4.588GluLeu: 4.588 ± 0.612
2.41GluMet: 2.41 ± 0.475
3.188GluAsn: 3.188 ± 0.416
1.711GluPro: 1.711 ± 0.547
2.41GluGln: 2.41 ± 0.666
3.11GluArg: 3.11 ± 0.631
1.788GluSer: 1.788 ± 0.371
4.043GluThr: 4.043 ± 0.609
3.888GluVal: 3.888 ± 0.543
0.778GluTrp: 0.778 ± 0.317
1.866GluTyr: 1.866 ± 0.419
0.0GluXaa: 0.0 ± 0.0
Phe
2.022PheAla: 2.022 ± 0.374
0.467PheCys: 0.467 ± 0.209
3.11PheAsp: 3.11 ± 0.544
1.944PheGlu: 1.944 ± 0.356
2.022PhePhe: 2.022 ± 0.321
2.488PheGly: 2.488 ± 0.423
0.933PheHis: 0.933 ± 0.292
2.799PheIle: 2.799 ± 0.375
3.732PheLys: 3.732 ± 0.56
2.877PheLeu: 2.877 ± 0.488
1.244PheMet: 1.244 ± 0.272
1.633PheAsn: 1.633 ± 0.402
0.855PhePro: 0.855 ± 0.224
1.711PheGln: 1.711 ± 0.32
1.244PheArg: 1.244 ± 0.303
3.421PheSer: 3.421 ± 0.463
3.266PheThr: 3.266 ± 0.472
2.099PheVal: 2.099 ± 0.369
0.467PheTrp: 0.467 ± 0.183
1.322PheTyr: 1.322 ± 0.353
0.0PheXaa: 0.0 ± 0.0
Gly
4.121GlyAla: 4.121 ± 0.625
0.311GlyCys: 0.311 ± 0.144
3.577GlyAsp: 3.577 ± 0.43
3.965GlyGlu: 3.965 ± 0.544
3.266GlyPhe: 3.266 ± 0.472
3.965GlyGly: 3.965 ± 0.675
1.089GlyHis: 1.089 ± 0.24
3.965GlyIle: 3.965 ± 0.6
5.754GlyLys: 5.754 ± 0.755
5.443GlyLeu: 5.443 ± 0.583
1.477GlyMet: 1.477 ± 0.346
3.81GlyAsn: 3.81 ± 0.877
0.0GlyPro: 0.0 ± 0.0
2.41GlyGln: 2.41 ± 0.472
2.255GlyArg: 2.255 ± 0.507
5.598GlySer: 5.598 ± 1.16
6.609GlyThr: 6.609 ± 1.296
4.821GlyVal: 4.821 ± 0.501
1.166GlyTrp: 1.166 ± 0.266
2.644GlyTyr: 2.644 ± 0.691
0.0GlyXaa: 0.0 ± 0.0
His
1.4HisAla: 1.4 ± 0.297
0.311HisCys: 0.311 ± 0.137
0.7HisAsp: 0.7 ± 0.212
0.544HisGlu: 0.544 ± 0.254
0.855HisPhe: 0.855 ± 0.254
1.166HisGly: 1.166 ± 0.249
0.389HisHis: 0.389 ± 0.182
1.322HisIle: 1.322 ± 0.398
1.166HisLys: 1.166 ± 0.286
1.711HisLeu: 1.711 ± 0.483
0.622HisMet: 0.622 ± 0.187
0.778HisAsn: 0.778 ± 0.191
0.544HisPro: 0.544 ± 0.156
0.467HisGln: 0.467 ± 0.189
0.622HisArg: 0.622 ± 0.25
1.244HisSer: 1.244 ± 0.324
1.166HisThr: 1.166 ± 0.308
0.778HisVal: 0.778 ± 0.227
0.389HisTrp: 0.389 ± 0.165
0.544HisTyr: 0.544 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
5.909IleAla: 5.909 ± 0.633
0.622IleCys: 0.622 ± 0.241
4.043IleAsp: 4.043 ± 0.603
5.287IleGlu: 5.287 ± 0.734
1.866IlePhe: 1.866 ± 0.442
4.665IleGly: 4.665 ± 0.6
1.166IleHis: 1.166 ± 0.326
3.732IleIle: 3.732 ± 0.719
5.832IleLys: 5.832 ± 0.638
4.588IleLeu: 4.588 ± 0.464
1.711IleMet: 1.711 ± 0.374
4.276IleAsn: 4.276 ± 0.627
2.877IlePro: 2.877 ± 0.471
2.566IleGln: 2.566 ± 0.528
2.566IleArg: 2.566 ± 0.374
4.121IleSer: 4.121 ± 0.66
5.054IleThr: 5.054 ± 0.775
3.266IleVal: 3.266 ± 0.387
0.622IleTrp: 0.622 ± 0.209
1.788IleTyr: 1.788 ± 0.36
0.0IleXaa: 0.0 ± 0.0
Lys
5.365LysAla: 5.365 ± 0.855
0.544LysCys: 0.544 ± 0.209
5.365LysAsp: 5.365 ± 0.805
6.842LysGlu: 6.842 ± 0.831
3.654LysPhe: 3.654 ± 0.649
4.821LysGly: 4.821 ± 0.734
1.711LysHis: 1.711 ± 0.32
6.687LysIle: 6.687 ± 0.696
8.009LysLys: 8.009 ± 1.134
7.231LysLeu: 7.231 ± 0.899
1.633LysMet: 1.633 ± 0.383
4.354LysAsn: 4.354 ± 0.781
2.799LysPro: 2.799 ± 0.371
2.41LysGln: 2.41 ± 0.466
4.276LysArg: 4.276 ± 0.781
4.821LysSer: 4.821 ± 0.759
5.909LysThr: 5.909 ± 0.755
4.121LysVal: 4.121 ± 0.624
0.933LysTrp: 0.933 ± 0.325
2.955LysTyr: 2.955 ± 0.566
0.0LysXaa: 0.0 ± 0.0
Leu
6.687LeuAla: 6.687 ± 0.706
0.544LeuCys: 0.544 ± 0.298
4.976LeuAsp: 4.976 ± 0.649
5.365LeuGlu: 5.365 ± 0.713
2.644LeuPhe: 2.644 ± 0.468
3.888LeuGly: 3.888 ± 0.482
1.711LeuHis: 1.711 ± 0.377
4.121LeuIle: 4.121 ± 0.501
7.153LeuLys: 7.153 ± 0.634
6.22LeuLeu: 6.22 ± 0.854
1.944LeuMet: 1.944 ± 0.499
5.365LeuAsn: 5.365 ± 0.562
3.343LeuPro: 3.343 ± 0.409
3.266LeuGln: 3.266 ± 0.512
2.955LeuArg: 2.955 ± 0.465
5.054LeuSer: 5.054 ± 0.424
5.987LeuThr: 5.987 ± 0.747
4.199LeuVal: 4.199 ± 0.437
0.933LeuTrp: 0.933 ± 0.314
2.177LeuTyr: 2.177 ± 0.336
0.0LeuXaa: 0.0 ± 0.0
Met
3.11MetAla: 3.11 ± 0.544
0.389MetCys: 0.389 ± 0.202
0.7MetAsp: 0.7 ± 0.211
1.633MetGlu: 1.633 ± 0.409
0.467MetPhe: 0.467 ± 0.182
1.477MetGly: 1.477 ± 0.268
0.156MetHis: 0.156 ± 0.103
1.322MetIle: 1.322 ± 0.295
2.255MetLys: 2.255 ± 0.522
1.711MetLeu: 1.711 ± 0.483
0.778MetMet: 0.778 ± 0.217
1.244MetAsn: 1.244 ± 0.256
1.477MetPro: 1.477 ± 0.38
1.089MetGln: 1.089 ± 0.332
1.633MetArg: 1.633 ± 0.39
2.177MetSer: 2.177 ± 0.319
1.633MetThr: 1.633 ± 0.467
1.4MetVal: 1.4 ± 0.373
0.389MetTrp: 0.389 ± 0.173
0.7MetTyr: 0.7 ± 0.276
0.0MetXaa: 0.0 ± 0.0
Asn
5.132AsnAla: 5.132 ± 0.742
0.311AsnCys: 0.311 ± 0.144
3.188AsnAsp: 3.188 ± 0.547
3.499AsnGlu: 3.499 ± 0.524
2.955AsnPhe: 2.955 ± 0.521
5.21AsnGly: 5.21 ± 0.873
0.622AsnHis: 0.622 ± 0.244
4.121AsnIle: 4.121 ± 0.647
4.121AsnLys: 4.121 ± 0.466
5.443AsnLeu: 5.443 ± 0.478
1.166AsnMet: 1.166 ± 0.321
4.432AsnAsn: 4.432 ± 0.758
2.255AsnPro: 2.255 ± 0.442
2.255AsnGln: 2.255 ± 0.411
2.488AsnArg: 2.488 ± 0.457
4.276AsnSer: 4.276 ± 0.678
5.521AsnThr: 5.521 ± 0.749
3.188AsnVal: 3.188 ± 0.57
0.622AsnTrp: 0.622 ± 0.273
2.799AsnTyr: 2.799 ± 0.514
0.0AsnXaa: 0.0 ± 0.0
Pro
1.322ProAla: 1.322 ± 0.288
0.311ProCys: 0.311 ± 0.133
1.944ProAsp: 1.944 ± 0.306
2.799ProGlu: 2.799 ± 0.723
1.866ProPhe: 1.866 ± 0.317
0.0ProGly: 0.0 ± 0.0
0.389ProHis: 0.389 ± 0.167
1.866ProIle: 1.866 ± 0.437
2.877ProLys: 2.877 ± 0.554
2.488ProLeu: 2.488 ± 0.37
0.622ProMet: 0.622 ± 0.187
2.333ProAsn: 2.333 ± 0.348
1.089ProPro: 1.089 ± 0.332
1.322ProGln: 1.322 ± 0.314
0.467ProArg: 0.467 ± 0.17
2.877ProSer: 2.877 ± 0.428
2.721ProThr: 2.721 ± 0.536
1.244ProVal: 1.244 ± 0.337
0.078ProTrp: 0.078 ± 0.064
0.778ProTyr: 0.778 ± 0.258
0.0ProXaa: 0.0 ± 0.0
Gln
2.799GlnAla: 2.799 ± 0.504
0.156GlnCys: 0.156 ± 0.115
2.255GlnAsp: 2.255 ± 0.584
3.343GlnGlu: 3.343 ± 0.627
1.322GlnPhe: 1.322 ± 0.343
1.788GlnGly: 1.788 ± 0.237
0.467GlnHis: 0.467 ± 0.184
2.566GlnIle: 2.566 ± 0.409
3.188GlnLys: 3.188 ± 0.541
2.799GlnLeu: 2.799 ± 0.588
1.244GlnMet: 1.244 ± 0.452
1.788GlnAsn: 1.788 ± 0.444
0.467GlnPro: 0.467 ± 0.151
1.555GlnGln: 1.555 ± 0.336
1.555GlnArg: 1.555 ± 0.336
2.955GlnSer: 2.955 ± 0.57
2.177GlnThr: 2.177 ± 0.488
2.099GlnVal: 2.099 ± 0.435
0.467GlnTrp: 0.467 ± 0.138
1.477GlnTyr: 1.477 ± 0.302
0.0GlnXaa: 0.0 ± 0.0
Arg
3.266ArgAla: 3.266 ± 0.49
0.0ArgCys: 0.0 ± 0.0
2.955ArgAsp: 2.955 ± 0.506
2.644ArgGlu: 2.644 ± 0.53
1.089ArgPhe: 1.089 ± 0.283
2.566ArgGly: 2.566 ± 0.477
0.778ArgHis: 0.778 ± 0.248
2.255ArgIle: 2.255 ± 0.418
3.654ArgLys: 3.654 ± 0.767
3.343ArgLeu: 3.343 ± 0.722
1.166ArgMet: 1.166 ± 0.385
2.177ArgAsn: 2.177 ± 0.439
0.933ArgPro: 0.933 ± 0.226
1.322ArgGln: 1.322 ± 0.319
1.711ArgArg: 1.711 ± 0.398
2.177ArgSer: 2.177 ± 0.567
1.944ArgThr: 1.944 ± 0.394
1.555ArgVal: 1.555 ± 0.418
0.7ArgTrp: 0.7 ± 0.239
2.177ArgTyr: 2.177 ± 0.331
0.0ArgXaa: 0.0 ± 0.0
Ser
6.22SerAla: 6.22 ± 0.805
0.933SerCys: 0.933 ± 0.3
3.421SerAsp: 3.421 ± 0.486
3.732SerGlu: 3.732 ± 0.455
2.41SerPhe: 2.41 ± 0.37
5.987SerGly: 5.987 ± 1.08
1.4SerHis: 1.4 ± 0.3
5.676SerIle: 5.676 ± 0.633
5.287SerLys: 5.287 ± 0.629
4.976SerLeu: 4.976 ± 0.608
1.477SerMet: 1.477 ± 0.342
3.654SerAsn: 3.654 ± 0.625
1.711SerPro: 1.711 ± 0.333
2.721SerGln: 2.721 ± 0.418
1.944SerArg: 1.944 ± 0.466
5.054SerSer: 5.054 ± 0.894
5.054SerThr: 5.054 ± 0.921
4.199SerVal: 4.199 ± 0.604
1.011SerTrp: 1.011 ± 0.236
2.255SerTyr: 2.255 ± 0.334
0.0SerXaa: 0.0 ± 0.0
Thr
6.531ThrAla: 6.531 ± 0.814
0.622ThrCys: 0.622 ± 0.223
4.043ThrAsp: 4.043 ± 0.648
4.665ThrGlu: 4.665 ± 0.606
2.877ThrPhe: 2.877 ± 0.656
7.387ThrGly: 7.387 ± 1.052
0.933ThrHis: 0.933 ± 0.223
4.121ThrIle: 4.121 ± 0.464
5.054ThrLys: 5.054 ± 0.83
5.443ThrLeu: 5.443 ± 0.662
1.633ThrMet: 1.633 ± 0.396
5.676ThrAsn: 5.676 ± 1.143
2.644ThrPro: 2.644 ± 0.409
2.644ThrGln: 2.644 ± 0.515
2.333ThrArg: 2.333 ± 0.347
5.598ThrSer: 5.598 ± 1.109
6.687ThrThr: 6.687 ± 1.367
4.976ThrVal: 4.976 ± 1.21
0.855ThrTrp: 0.855 ± 0.286
2.41ThrTyr: 2.41 ± 0.373
0.0ThrXaa: 0.0 ± 0.0
Val
5.521ValAla: 5.521 ± 1.21
0.778ValCys: 0.778 ± 0.267
3.188ValAsp: 3.188 ± 0.4
3.032ValGlu: 3.032 ± 0.442
1.944ValPhe: 1.944 ± 0.311
3.577ValGly: 3.577 ± 0.505
1.4ValHis: 1.4 ± 0.376
4.199ValIle: 4.199 ± 0.529
3.81ValLys: 3.81 ± 0.675
3.654ValLeu: 3.654 ± 0.441
0.933ValMet: 0.933 ± 0.308
4.821ValAsn: 4.821 ± 0.841
1.555ValPro: 1.555 ± 0.338
1.788ValGln: 1.788 ± 0.293
2.099ValArg: 2.099 ± 0.318
4.276ValSer: 4.276 ± 0.59
4.588ValThr: 4.588 ± 0.842
2.721ValVal: 2.721 ± 0.373
0.7ValTrp: 0.7 ± 0.275
1.788ValTyr: 1.788 ± 0.454
0.0ValXaa: 0.0 ± 0.0
Trp
0.622TrpAla: 0.622 ± 0.263
0.078TrpCys: 0.078 ± 0.076
1.011TrpAsp: 1.011 ± 0.213
0.311TrpGlu: 0.311 ± 0.168
0.467TrpPhe: 0.467 ± 0.14
0.544TrpGly: 0.544 ± 0.176
0.0TrpHis: 0.0 ± 0.0
0.311TrpIle: 0.311 ± 0.192
1.244TrpLys: 1.244 ± 0.31
1.555TrpLeu: 1.555 ± 0.404
0.0TrpMet: 0.0 ± 0.0
1.477TrpAsn: 1.477 ± 0.299
0.0TrpPro: 0.0 ± 0.0
0.7TrpGln: 0.7 ± 0.271
0.622TrpArg: 0.622 ± 0.279
1.089TrpSer: 1.089 ± 0.274
1.244TrpThr: 1.244 ± 0.243
1.166TrpVal: 1.166 ± 0.246
0.078TrpTrp: 0.078 ± 0.07
0.389TrpTyr: 0.389 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.633TyrAla: 1.633 ± 0.347
0.855TyrCys: 0.855 ± 0.271
1.633TyrAsp: 1.633 ± 0.266
1.089TyrGlu: 1.089 ± 0.246
1.322TyrPhe: 1.322 ± 0.596
2.566TyrGly: 2.566 ± 0.422
0.7TyrHis: 0.7 ± 0.176
1.944TyrIle: 1.944 ± 0.517
3.888TyrLys: 3.888 ± 0.606
2.644TyrLeu: 2.644 ± 0.468
0.855TyrMet: 0.855 ± 0.218
2.644TyrAsn: 2.644 ± 0.404
0.622TyrPro: 0.622 ± 0.213
1.633TyrGln: 1.633 ± 0.358
0.7TyrArg: 0.7 ± 0.188
2.877TyrSer: 2.877 ± 0.48
2.644TyrThr: 2.644 ± 0.473
2.877TyrVal: 2.877 ± 0.533
0.7TyrTrp: 0.7 ± 0.196
1.089TyrTyr: 1.089 ± 0.229
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (12862 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski