Microbacterium sp. SUBG005

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A074TP36|A0A074TP36_9MICO Uncharacterized protein (Fragment) OS=Microbacterium sp. SUBG005 OX=1504156 GN=HR12_13855 PE=4 SV=1
YYY2 pKa = 9.89AEEE5 pKa = 4.01VKKK8 pKa = 8.59TNGVSDDD15 pKa = 3.79VTWSGLEEE23 pKa = 3.73GNGKKK28 pKa = 9.7EE29 pKa = 4.47YYY31 pKa = 10.29LTAEEE36 pKa = 4.59ATGGHHH42 pKa = 6.35GTITGTGYYY51 pKa = 10.94DDD53 pKa = 3.2FIAEEE58 pKa = 4.68DD59 pKa = 3.31TYYY62 pKa = 9.71YYY64 pKa = 10.88GSGGWNTHHH73 pKa = 5.26DDD75 pKa = 3.42YY76 pKa = 10.81DD77 pKa = 3.69WSNTGGMDDD86 pKa = 3.57VDDD89 pKa = 3.99YY90 pKa = 11.29NATSGITVDDD100 pKa = 4.65RR101 pKa = 11.84LSTAQDDD108 pKa = 3.01GFGTTRR114 pKa = 11.84LLNIEEE120 pKa = 4.88INGSDDD126 pKa = 3.82YY127 pKa = 11.66DD128 pKa = 5.02DD129 pKa = 5.05ITGNSGDDD137 pKa = 3.65QFEEE141 pKa = 4.28RR142 pKa = 11.84GGNDDD147 pKa = 2.96FNIGSGGHHH156 pKa = 6.15DD157 pKa = 3.21LLYYY161 pKa = 10.78KK162 pKa = 10.76INASDDD168 pKa = 3.59TGGNGSDDD176 pKa = 3.46VNGFTVGTWEEE187 pKa = 4.2TADDD191 pKa = 3.59DDD193 pKa = 5.43IDDD196 pKa = 5.4RR197 pKa = 11.84DDD199 pKa = 3.72LSDDD203 pKa = 3.57GYYY206 pKa = 9.2GTGSASYYY214 pKa = 10.8NGVATLDDD222 pKa = 3.59SAGNIADDD230 pKa = 4.21YY231 pKa = 10.95RR232 pKa = 11.84VVQNGSNTEEE242 pKa = 3.85QVDDD246 pKa = 3.87DDD248 pKa = 3.68TGGQFTPTTLVTLNGXQTDDD268 pKa = 4.13ATLLANHHH276 pKa = 6.26LLIA

Molecular weight: 28.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A074TZG7|A0A074TZG7_9MICO ATPase AAA OS=Microbacterium sp. SUBG005 OX=1504156 GN=HR12_23780 PE=4 SV=1
MM1 pKa = 7.13VFSSVASSSGMRR13 pKa = 11.84KK14 pKa = 9.32VNPSISTAPAAMLTAPIARR33 pKa = 11.84RR34 pKa = 11.84SFKK37 pKa = 10.44RR38 pKa = 11.84RR39 pKa = 11.84APAHH43 pKa = 5.46MRR45 pKa = 11.84PTATRR50 pKa = 11.84LVALPTAAAVSPPPAVSRR68 pKa = 11.84TFIAPQSRR76 pKa = 11.84AAMAATPKK84 pKa = 10.69

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4595

0

4595

895786

33

1291

194.9

21.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.852 ± 0.061

0.604 ± 0.011

6.264 ± 0.038

5.435 ± 0.036

3.409 ± 0.026

8.672 ± 0.04

2.042 ± 0.02

4.608 ± 0.034

2.286 ± 0.031

9.996 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.043 ± 0.022

2.219 ± 0.025

5.134 ± 0.031

3.033 ± 0.028

7.32 ± 0.047

5.658 ± 0.031

5.968 ± 0.03

8.854 ± 0.037

1.491 ± 0.018

2.079 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski