Amino acid dipepetide frequency for Microbacterium sp. SUBG005

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.635AlaAla: 18.635 ± 0.182	0.758AlaCys: 0.758 ± 0.026	7.974AlaAsp: 7.974 ± 0.113	7.405AlaGlu: 7.405 ± 0.111	4.005AlaPhe: 4.005 ± 0.075	11.105AlaGly: 11.105 ± 0.129	2.531AlaHis: 2.531 ± 0.06	5.598AlaIle: 5.598 ± 0.086	2.889AlaLys: 2.889 ± 0.068	13.584AlaLeu: 13.584 ± 0.146	2.899AlaMet: 2.899 ± 0.058	2.377AlaAsn: 2.377 ± 0.058	5.91AlaPro: 5.91 ± 0.086	3.911AlaGln: 3.911 ± 0.062	9.075AlaArg: 9.075 ± 0.121	7.298AlaSer: 7.298 ± 0.084	6.978AlaThr: 6.978 ± 0.084	11.309AlaVal: 11.309 ± 0.134	1.837AlaTrp: 1.837 ± 0.05	2.405AlaTyr: 2.405 ± 0.051	0.038AlaXaa: 0.038 ± 0.006
Cys	0.77CysAla: 0.77 ± 0.028	0.086CysCys: 0.086 ± 0.01	0.34CysAsp: 0.34 ± 0.017	0.282CysGlu: 0.282 ± 0.019	0.231CysPhe: 0.231 ± 0.016	0.664CysGly: 0.664 ± 0.029	0.17CysHis: 0.17 ± 0.015	0.231CysIle: 0.231 ± 0.018	0.097CysLys: 0.097 ± 0.011	0.501CysLeu: 0.501 ± 0.026	0.113CysMet: 0.113 ± 0.011	0.124CysAsn: 0.124 ± 0.012	0.329CysPro: 0.329 ± 0.021	0.193CysGln: 0.193 ± 0.014	0.461CysArg: 0.461 ± 0.021	0.426CysSer: 0.426 ± 0.022	0.357CysThr: 0.357 ± 0.019	0.452CysVal: 0.452 ± 0.023	0.09CysTrp: 0.09 ± 0.009	0.122CysTyr: 0.122 ± 0.013	0.001CysXaa: 0.001 ± 0.001
Asp	9.288AspAla: 9.288 ± 0.112	0.263AspCys: 0.263 ± 0.018	4.893AspAsp: 4.893 ± 0.078	4.271AspGlu: 4.271 ± 0.076	1.804AspPhe: 1.804 ± 0.043	5.844AspGly: 5.844 ± 0.092	1.25AspHis: 1.25 ± 0.037	2.616AspIle: 2.616 ± 0.059	1.198AspLys: 1.198 ± 0.042	6.192AspLeu: 6.192 ± 0.084	0.975AspMet: 0.975 ± 0.03	1.163AspAsn: 1.163 ± 0.037	3.723AspPro: 3.723 ± 0.069	1.608AspGln: 1.608 ± 0.042	4.34AspArg: 4.34 ± 0.072	2.482AspSer: 2.482 ± 0.046	3.087AspThr: 3.087 ± 0.058	5.401AspVal: 5.401 ± 0.091	0.855AspTrp: 0.855 ± 0.032	1.378AspTyr: 1.378 ± 0.039	0.013AspXaa: 0.013 ± 0.004
Glu	6.497GluAla: 6.497 ± 0.097	0.249GluCys: 0.249 ± 0.018	2.411GluAsp: 2.411 ± 0.063	2.905GluGlu: 2.905 ± 0.058	1.656GluPhe: 1.656 ± 0.038	4.106GluGly: 4.106 ± 0.073	1.352GluHis: 1.352 ± 0.039	2.857GluIle: 2.857 ± 0.057	1.685GluLys: 1.685 ± 0.049	5.773GluLeu: 5.773 ± 0.083	1.114GluMet: 1.114 ± 0.037	1.47GluAsn: 1.47 ± 0.039	2.746GluPro: 2.746 ± 0.057	2.281GluGln: 2.281 ± 0.051	4.943GluArg: 4.943 ± 0.086	2.594GluSer: 2.594 ± 0.048	3.021GluThr: 3.021 ± 0.057	4.642GluVal: 4.642 ± 0.074	0.871GluTrp: 0.871 ± 0.036	1.162GluTyr: 1.162 ± 0.037	0.015GluXaa: 0.015 ± 0.004
Phe	4.525PheAla: 4.525 ± 0.077	0.236PheCys: 0.236 ± 0.018	2.55PheAsp: 2.55 ± 0.053	1.689PheGlu: 1.689 ± 0.047	1.361PhePhe: 1.361 ± 0.048	3.407PheGly: 3.407 ± 0.062	0.621PheHis: 0.621 ± 0.03	1.426PheIle: 1.426 ± 0.047	0.567PheLys: 0.567 ± 0.024	3.183PheLeu: 3.183 ± 0.072	0.541PheMet: 0.541 ± 0.025	0.88PheAsn: 0.88 ± 0.039	1.423PhePro: 1.423 ± 0.044	0.948PheGln: 0.948 ± 0.033	1.968PheArg: 1.968 ± 0.053	2.151PheSer: 2.151 ± 0.054	2.316PheThr: 2.316 ± 0.052	2.965PheVal: 2.965 ± 0.07	0.529PheTrp: 0.529 ± 0.027	0.789PheTyr: 0.789 ± 0.032	0.018PheXaa: 0.018 ± 0.006
Gly	9.849GlyAla: 9.849 ± 0.119	0.672GlyCys: 0.672 ± 0.03	5.097GlyAsp: 5.097 ± 0.076	4.891GlyGlu: 4.891 ± 0.079	3.462GlyPhe: 3.462 ± 0.071	7.367GlyGly: 7.367 ± 0.103	1.781GlyHis: 1.781 ± 0.047	4.884GlyIle: 4.884 ± 0.081	2.389GlyLys: 2.389 ± 0.058	8.459GlyLeu: 8.459 ± 0.1	2.04GlyMet: 2.04 ± 0.049	1.902GlyAsn: 1.902 ± 0.043	3.544GlyPro: 3.544 ± 0.078	2.487GlyGln: 2.487 ± 0.06	6.228GlyArg: 6.228 ± 0.102	5.049GlySer: 5.049 ± 0.075	5.069GlyThr: 5.069 ± 0.08	7.744GlyVal: 7.744 ± 0.1	1.586GlyTrp: 1.586 ± 0.047	2.196GlyTyr: 2.196 ± 0.047	0.023GlyXaa: 0.023 ± 0.005
His	2.302HisAla: 2.302 ± 0.055	0.15HisCys: 0.15 ± 0.011	1.336HisAsp: 1.336 ± 0.045	1.093HisGlu: 1.093 ± 0.035	0.681HisPhe: 0.681 ± 0.03	1.932HisGly: 1.932 ± 0.056	0.603HisHis: 0.603 ± 0.026	0.826HisIle: 0.826 ± 0.031	0.357HisLys: 0.357 ± 0.019	2.104HisLeu: 2.104 ± 0.046	0.391HisMet: 0.391 ± 0.022	0.378HisAsn: 0.378 ± 0.017	1.498HisPro: 1.498 ± 0.039	0.618HisGln: 0.618 ± 0.028	1.641HisArg: 1.641 ± 0.042	0.953HisSer: 0.953 ± 0.028	1.008HisThr: 1.008 ± 0.032	1.74HisVal: 1.74 ± 0.044	0.297HisTrp: 0.297 ± 0.02	0.501HisTyr: 0.501 ± 0.025	0.01HisXaa: 0.01 ± 0.003
Ile	6.895IleAla: 6.895 ± 0.096	0.28IleCys: 0.28 ± 0.017	3.474IleAsp: 3.474 ± 0.072	2.698IleGlu: 2.698 ± 0.057	1.438IlePhe: 1.438 ± 0.047	4.465IleGly: 4.465 ± 0.088	0.761IleHis: 0.761 ± 0.027	1.942IleIle: 1.942 ± 0.049	0.979IleLys: 0.979 ± 0.033	3.761IleLeu: 3.761 ± 0.073	0.721IleMet: 0.721 ± 0.027	1.13IleAsn: 1.13 ± 0.039	2.418IlePro: 2.418 ± 0.056	1.131IleGln: 1.131 ± 0.033	2.726IleArg: 2.726 ± 0.059	2.463IleSer: 2.463 ± 0.05	2.728IleThr: 2.728 ± 0.051	4.74IleVal: 4.74 ± 0.086	0.514IleTrp: 0.514 ± 0.024	0.81IleTyr: 0.81 ± 0.032	0.008IleXaa: 0.008 ± 0.003
Lys	2.658LysAla: 2.658 ± 0.068	0.089LysCys: 0.089 ± 0.011	1.325LysAsp: 1.325 ± 0.044	1.128LysGlu: 1.128 ± 0.039	0.654LysPhe: 0.654 ± 0.031	1.779LysGly: 1.779 ± 0.046	0.445LysHis: 0.445 ± 0.023	1.154LysIle: 1.154 ± 0.035	0.989LysLys: 0.989 ± 0.039	2.203LysLeu: 2.203 ± 0.056	0.5LysMet: 0.5 ± 0.023	0.652LysAsn: 0.652 ± 0.029	1.25LysPro: 1.25 ± 0.041	0.911LysGln: 0.911 ± 0.033	1.682LysArg: 1.682 ± 0.045	1.2LysSer: 1.2 ± 0.04	1.474LysThr: 1.474 ± 0.045	1.919LysVal: 1.919 ± 0.05	0.275LysTrp: 0.275 ± 0.016	0.558LysTyr: 0.558 ± 0.024	0.018LysXaa: 0.018 ± 0.005
Leu	13.662LeuAla: 13.662 ± 0.14	0.589LeuCys: 0.589 ± 0.024	6.374LeuAsp: 6.374 ± 0.085	5.044LeuGlu: 5.044 ± 0.078	3.385LeuPhe: 3.385 ± 0.079	8.548LeuGly: 8.548 ± 0.105	1.902LeuHis: 1.902 ± 0.051	4.405LeuIle: 4.405 ± 0.08	2.109LeuLys: 2.109 ± 0.052	10.159LeuLeu: 10.159 ± 0.139	1.939LeuMet: 1.939 ± 0.052	2.141LeuAsn: 2.141 ± 0.053	5.303LeuPro: 5.303 ± 0.083	2.806LeuGln: 2.806 ± 0.055	7.315LeuArg: 7.315 ± 0.1	6.114LeuSer: 6.114 ± 0.086	6.095LeuThr: 6.095 ± 0.082	8.85LeuVal: 8.85 ± 0.103	1.336LeuTrp: 1.336 ± 0.043	1.861LeuTyr: 1.861 ± 0.049	0.027LeuXaa: 0.027 ± 0.006
Met	2.261MetAla: 2.261 ± 0.062	0.121MetCys: 0.121 ± 0.012	0.923MetAsp: 0.923 ± 0.036	0.729MetGlu: 0.729 ± 0.029	0.635MetPhe: 0.635 ± 0.026	1.544MetGly: 1.544 ± 0.045	0.354MetHis: 0.354 ± 0.02	1.102MetIle: 1.102 ± 0.039	0.597MetLys: 0.597 ± 0.028	2.059MetLeu: 2.059 ± 0.051	0.503MetMet: 0.503 ± 0.028	0.58MetAsn: 0.58 ± 0.024	1.27MetPro: 1.27 ± 0.038	0.75MetGln: 0.75 ± 0.026	1.443MetArg: 1.443 ± 0.041	1.632MetSer: 1.632 ± 0.043	1.86MetThr: 1.86 ± 0.048	1.485MetVal: 1.485 ± 0.048	0.244MetTrp: 0.244 ± 0.018	0.335MetTyr: 0.335 ± 0.02	0.006MetXaa: 0.006 ± 0.003
Asn	2.685AsnAla: 2.685 ± 0.062	0.122AsnCys: 0.122 ± 0.012	1.352AsnAsp: 1.352 ± 0.041	1.121AsnGlu: 1.121 ± 0.034	0.798AsnPhe: 0.798 ± 0.031	2.117AsnGly: 2.117 ± 0.056	0.423AsnHis: 0.423 ± 0.023	1.093AsnIle: 1.093 ± 0.04	0.595AsnLys: 0.595 ± 0.027	2.079AsnLeu: 2.079 ± 0.046	0.435AsnMet: 0.435 ± 0.02	0.637AsnAsn: 0.637 ± 0.027	1.611AsnPro: 1.611 ± 0.041	0.721AsnGln: 0.721 ± 0.028	1.363AsnArg: 1.363 ± 0.04	1.02AsnSer: 1.02 ± 0.037	1.341AsnThr: 1.341 ± 0.045	1.848AsnVal: 1.848 ± 0.047	0.322AsnTrp: 0.322 ± 0.02	0.505AsnTyr: 0.505 ± 0.023	0.001AsnXaa: 0.001 ± 0.001
Pro	6.681ProAla: 6.681 ± 0.114	0.255ProCys: 0.255 ± 0.017	3.738ProAsp: 3.738 ± 0.063	3.406ProGlu: 3.406 ± 0.064	1.855ProPhe: 1.855 ± 0.043	4.608ProGly: 4.608 ± 0.079	1.164ProHis: 1.164 ± 0.037	1.846ProIle: 1.846 ± 0.048	1.039ProLys: 1.039 ± 0.036	4.819ProLeu: 4.819 ± 0.073	1.006ProMet: 1.006 ± 0.032	0.971ProAsn: 0.971 ± 0.033	2.171ProPro: 2.171 ± 0.061	1.469ProGln: 1.469 ± 0.034	3.641ProArg: 3.641 ± 0.069	3.059ProSer: 3.059 ± 0.057	3.19ProThr: 3.19 ± 0.062	4.584ProVal: 4.584 ± 0.077	0.828ProTrp: 0.828 ± 0.027	0.995ProTyr: 0.995 ± 0.033	0.016ProXaa: 0.016 ± 0.004
Gln	3.558GlnAla: 3.558 ± 0.066	0.143GlnCys: 0.143 ± 0.013	1.333GlnAsp: 1.333 ± 0.042	1.366GlnGlu: 1.366 ± 0.041	1.002GlnPhe: 1.002 ± 0.031	2.254GlnGly: 2.254 ± 0.054	0.706GlnHis: 0.706 ± 0.029	1.609GlnIle: 1.609 ± 0.039	0.892GlnLys: 0.892 ± 0.032	3.348GlnLeu: 3.348 ± 0.064	0.634GlnMet: 0.634 ± 0.026	0.857GlnAsn: 0.857 ± 0.032	1.523GlnPro: 1.523 ± 0.041	1.471GlnGln: 1.471 ± 0.044	2.563GlnArg: 2.563 ± 0.057	1.597GlnSer: 1.597 ± 0.049	1.72GlnThr: 1.72 ± 0.052	2.472GlnVal: 2.472 ± 0.052	0.551GlnTrp: 0.551 ± 0.029	0.716GlnTyr: 0.716 ± 0.028	0.017GlnXaa: 0.017 ± 0.004
Arg	8.625ArgAla: 8.625 ± 0.117	0.447ArgCys: 0.447 ± 0.026	4.373ArgAsp: 4.373 ± 0.074	4.405ArgGlu: 4.405 ± 0.082	2.575ArgPhe: 2.575 ± 0.055	5.565ArgGly: 5.565 ± 0.083	1.689ArgHis: 1.689 ± 0.049	3.467ArgIle: 3.467 ± 0.067	1.518ArgLys: 1.518 ± 0.046	7.196ArgLeu: 7.196 ± 0.11	1.81ArgMet: 1.81 ± 0.049	1.44ArgAsn: 1.44 ± 0.04	3.607ArgPro: 3.607 ± 0.062	2.18ArgGln: 2.18 ± 0.054	6.913ArgArg: 6.913 ± 0.106	4.31ArgSer: 4.31 ± 0.071	4.154ArgThr: 4.154 ± 0.069	6.154ArgVal: 6.154 ± 0.091	1.187ArgTrp: 1.187 ± 0.038	1.575ArgTyr: 1.575 ± 0.048	0.009ArgXaa: 0.009 ± 0.003
Ser	6.965SerAla: 6.965 ± 0.105	0.394SerCys: 0.394 ± 0.021	3.316SerAsp: 3.316 ± 0.057	2.617SerGlu: 2.617 ± 0.051	2.05SerPhe: 2.05 ± 0.05	5.533SerGly: 5.533 ± 0.07	1.013SerHis: 1.013 ± 0.038	2.597SerIle: 2.597 ± 0.062	1.163SerLys: 1.163 ± 0.036	5.536SerLeu: 5.536 ± 0.082	1.298SerMet: 1.298 ± 0.039	1.176SerAsn: 1.176 ± 0.036	3.131SerPro: 3.131 ± 0.05	1.564SerGln: 1.564 ± 0.042	3.919SerArg: 3.919 ± 0.081	3.726SerSer: 3.726 ± 0.076	3.703SerThr: 3.703 ± 0.063	4.695SerVal: 4.695 ± 0.072	0.942SerTrp: 0.942 ± 0.034	1.231SerTyr: 1.231 ± 0.045	0.012SerXaa: 0.012 ± 0.004
Thr	7.563ThrAla: 7.563 ± 0.102	0.388ThrCys: 0.388 ± 0.022	3.566ThrAsp: 3.566 ± 0.067	2.714ThrGlu: 2.714 ± 0.057	2.112ThrPhe: 2.112 ± 0.056	5.581ThrGly: 5.581 ± 0.082	1.176ThrHis: 1.176 ± 0.036	2.641ThrIle: 2.641 ± 0.057	1.154ThrLys: 1.154 ± 0.041	6.327ThrLeu: 6.327 ± 0.093	1.078ThrMet: 1.078 ± 0.038	1.216ThrAsn: 1.216 ± 0.042	3.733ThrPro: 3.733 ± 0.061	1.592ThrGln: 1.592 ± 0.041	3.903ThrArg: 3.903 ± 0.071	3.351ThrSer: 3.351 ± 0.066	3.721ThrThr: 3.721 ± 0.073	5.698ThrVal: 5.698 ± 0.078	0.877ThrTrp: 0.877 ± 0.037	1.267ThrTyr: 1.267 ± 0.044	0.019ThrXaa: 0.019 ± 0.005
Val	11.168ValAla: 11.168 ± 0.125	0.538ValCys: 0.538 ± 0.024	5.942ValAsp: 5.942 ± 0.087	4.813ValGlu: 4.813 ± 0.082	3.002ValPhe: 3.002 ± 0.057	7.126ValGly: 7.126 ± 0.1	1.727ValHis: 1.727 ± 0.04	4.302ValIle: 4.302 ± 0.072	1.83ValLys: 1.83 ± 0.05	8.989ValLeu: 8.989 ± 0.121	1.681ValMet: 1.681 ± 0.049	2.046ValAsn: 2.046 ± 0.054	4.408ValPro: 4.408 ± 0.066	2.368ValGln: 2.368 ± 0.055	6.115ValArg: 6.115 ± 0.095	5.161ValSer: 5.161 ± 0.07	5.689ValThr: 5.689 ± 0.077	8.892ValVal: 8.892 ± 0.114	1.155ValTrp: 1.155 ± 0.038	1.57ValTyr: 1.57 ± 0.045	0.022ValXaa: 0.022 ± 0.006
Trp	1.472TrpAla: 1.472 ± 0.046	0.121TrpCys: 0.121 ± 0.012	0.776TrpAsp: 0.776 ± 0.029	0.668TrpGlu: 0.668 ± 0.028	0.607TrpPhe: 0.607 ± 0.025	1.133TrpGly: 1.133 ± 0.039	0.377TrpHis: 0.377 ± 0.02	0.713TrpIle: 0.713 ± 0.024	0.367TrpLys: 0.367 ± 0.02	1.605TrpLeu: 1.605 ± 0.045	0.392TrpMet: 0.392 ± 0.023	0.483TrpAsn: 0.483 ± 0.024	0.703TrpPro: 0.703 ± 0.028	0.591TrpGln: 0.591 ± 0.028	1.314TrpArg: 1.314 ± 0.041	0.896TrpSer: 0.896 ± 0.034	0.903TrpThr: 0.903 ± 0.035	1.169TrpVal: 1.169 ± 0.037	0.309TrpTrp: 0.309 ± 0.019	0.303TrpTyr: 0.303 ± 0.017	0.004TrpXaa: 0.004 ± 0.002
Tyr	2.428TyrAla: 2.428 ± 0.052	0.143TyrCys: 0.143 ± 0.013	1.531TyrAsp: 1.531 ± 0.042	1.093TyrGlu: 1.093 ± 0.037	0.76TyrPhe: 0.76 ± 0.028	2.016TyrGly: 2.016 ± 0.052	0.367TyrHis: 0.367 ± 0.023	0.762TyrIle: 0.762 ± 0.034	0.438TyrLys: 0.438 ± 0.023	2.071TyrLeu: 2.071 ± 0.043	0.357TyrMet: 0.357 ± 0.023	0.556TyrAsn: 0.556 ± 0.029	1.025TyrPro: 1.025 ± 0.036	0.719TyrGln: 0.719 ± 0.029	1.629TyrArg: 1.629 ± 0.049	1.082TyrSer: 1.082 ± 0.036	1.246TyrThr: 1.246 ± 0.037	1.76TyrVal: 1.76 ± 0.044	0.292TyrTrp: 0.292 ± 0.019	0.51TyrTyr: 0.51 ± 0.022	0.004TyrXaa: 0.004 ± 0.002
Xaa	0.033XaaAla: 0.033 ± 0.006	0.0XaaCys: 0.0 ± 0.0	0.02XaaAsp: 0.02 ± 0.005	0.012XaaGlu: 0.012 ± 0.004	0.018XaaPhe: 0.018 ± 0.004	0.026XaaGly: 0.026 ± 0.006	0.011XaaHis: 0.011 ± 0.004	0.01XaaIle: 0.01 ± 0.003	0.011XaaLys: 0.011 ± 0.003	0.019XaaLeu: 0.019 ± 0.005	0.004XaaMet: 0.004 ± 0.002	0.007XaaAsn: 0.007 ± 0.002	0.015XaaPro: 0.015 ± 0.004	0.013XaaGln: 0.013 ± 0.004	0.015XaaArg: 0.015 ± 0.004	0.015XaaSer: 0.015 ± 0.004	0.017XaaThr: 0.017 ± 0.004	0.026XaaVal: 0.026 ± 0.006	0.008XaaTrp: 0.008 ± 0.003	0.002XaaTyr: 0.002 ± 0.001	0.028XaaXaa: 0.028 ± 0.007

Statistics based on 4595 proteins (895786 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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