Achromobacter phage phiAxp-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rothmandenesvirinae; Jwalphavirus; Achromobacter virus Axp3

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FHI7|A0A0K2FHI7_9CAUD Uncharacterized protein OS=Achromobacter phage phiAxp-3 OX=1664247 GN=ADP65_00073 PE=4 SV=1
MM1 pKa = 7.25GVAIDD6 pKa = 4.54MATQDD11 pKa = 3.98AVVAMPEE18 pKa = 3.82AALEE22 pKa = 4.06EE23 pKa = 4.15QQVLIRR29 pKa = 11.84QMQAEE34 pKa = 5.06LVAEE38 pKa = 4.28MPGLILGAHH47 pKa = 5.48TVQYY51 pKa = 10.7GPNDD55 pKa = 3.44YY56 pKa = 11.02AFEE59 pKa = 4.63PGIGQIHH66 pKa = 6.18YY67 pKa = 10.63LPATSQRR74 pKa = 11.84ICKK77 pKa = 10.12DD78 pKa = 3.05LAQSYY83 pKa = 9.65LALIGDD89 pKa = 4.35YY90 pKa = 10.82PLVSPIKK97 pKa = 8.47PTRR100 pKa = 11.84DD101 pKa = 2.9SYY103 pKa = 11.9EE104 pKa = 3.76FVGRR108 pKa = 11.84LGIDD112 pKa = 3.22PVNEE116 pKa = 4.06PEE118 pKa = 4.18PVNWPTMEE126 pKa = 3.91YY127 pKa = 10.09YY128 pKa = 10.45RR129 pKa = 11.84RR130 pKa = 11.84FLRR133 pKa = 11.84LLHH136 pKa = 5.83EE137 pKa = 4.82RR138 pKa = 11.84NFKK141 pKa = 10.11FVNSVAYY148 pKa = 9.71EE149 pKa = 3.76ILDD152 pKa = 3.38FFMPEE157 pKa = 3.28EE158 pKa = 3.95WKK160 pKa = 10.19QRR162 pKa = 11.84NWLGLPAQSGWYY174 pKa = 8.25PPSSFIQPANEE185 pKa = 4.0APLDD189 pKa = 4.09YY190 pKa = 10.1IAKK193 pKa = 9.63VQIQILKK200 pKa = 9.31AGQEE204 pKa = 4.04IGMVPYY210 pKa = 9.77FQIGEE215 pKa = 4.17PWWWDD220 pKa = 2.96GSYY223 pKa = 11.66NNGEE227 pKa = 4.42GKK229 pKa = 9.65NAPCLYY235 pKa = 10.19DD236 pKa = 3.57QRR238 pKa = 11.84TMDD241 pKa = 4.67LYY243 pKa = 11.12KK244 pKa = 10.42QEE246 pKa = 4.32TGNDD250 pKa = 3.01VPMPMIKK257 pKa = 10.3SIFDD261 pKa = 3.7PVADD265 pKa = 4.15NQWPYY270 pKa = 11.04IDD272 pKa = 3.58WLRR275 pKa = 11.84TKK277 pKa = 10.8LGDD280 pKa = 3.28STNYY284 pKa = 9.59VRR286 pKa = 11.84DD287 pKa = 3.55KK288 pKa = 11.34VKK290 pKa = 10.67AAFPNAQATLLFFSPQVMSPASDD313 pKa = 3.15LTFRR317 pKa = 11.84LNFPMDD323 pKa = 2.86EE324 pKa = 3.99WVYY327 pKa = 10.58PNYY330 pKa = 10.58EE331 pKa = 3.88FVQIEE336 pKa = 4.4DD337 pKa = 3.92YY338 pKa = 11.37DD339 pKa = 4.01WIIDD343 pKa = 3.74GRR345 pKa = 11.84LDD347 pKa = 4.43LVPQTFTAATEE358 pKa = 3.94LLKK361 pKa = 11.21YY362 pKa = 9.41PLEE365 pKa = 4.22VVHH368 pKa = 6.01YY369 pKa = 8.29FIGFTLLPEE378 pKa = 4.2DD379 pKa = 5.1AKK381 pKa = 11.07RR382 pKa = 11.84IWGNTDD388 pKa = 3.21KK389 pKa = 11.24AWALAQEE396 pKa = 4.39AGIKK400 pKa = 9.72IIYY403 pKa = 7.48PWSYY407 pKa = 7.66TQVMRR412 pKa = 11.84DD413 pKa = 3.08GVYY416 pKa = 9.15FARR419 pKa = 11.84PKK421 pKa = 10.56VCGCC425 pKa = 4.1

Molecular weight: 48.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2FI96|A0A0K2FI96_9CAUD Uncharacterized protein OS=Achromobacter phage phiAxp-3 OX=1664247 GN=ADP65_00026 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 5.41KK3 pKa = 10.21NRR5 pKa = 11.84RR6 pKa = 11.84NAIIEE11 pKa = 4.41KK12 pKa = 10.09IEE14 pKa = 3.92ARR16 pKa = 11.84CDD18 pKa = 3.26IVDD21 pKa = 3.37MGFVIGMKK29 pKa = 10.16PSPCHH34 pKa = 6.1LWQGPTSGNGRR45 pKa = 11.84GGGYY49 pKa = 10.88GRR51 pKa = 11.84MSLNGHH57 pKa = 5.49TVAVHH62 pKa = 5.85LVVFTHH68 pKa = 5.85YY69 pKa = 10.42FGYY72 pKa = 10.25IPGNKK77 pKa = 8.97QVDD80 pKa = 3.82HH81 pKa = 6.98LCNQRR86 pKa = 11.84LCCNPQHH93 pKa = 6.7LEE95 pKa = 4.09LVTHH99 pKa = 6.71LTNQRR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84AARR109 pKa = 11.84SKK111 pKa = 10.85SS112 pKa = 3.34

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

22633

39

3428

282.9

31.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.517 ± 0.614

0.742 ± 0.131

5.828 ± 0.151

6.292 ± 0.184

3.636 ± 0.196

7.171 ± 0.236

1.94 ± 0.173

5.112 ± 0.214

6.027 ± 0.237

8.474 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.358 ± 0.131

4.71 ± 0.165

4.617 ± 0.224

4.975 ± 0.216

4.918 ± 0.182

5.465 ± 0.21

5.912 ± 0.209

6.689 ± 0.209

1.458 ± 0.182

3.159 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski