Amino acid dipepetide frequency for Achromobacter phage phiAxp-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.046AlaAla: 11.046 ± 1.165
0.619AlaCys: 0.619 ± 0.189
4.374AlaAsp: 4.374 ± 0.541
6.981AlaGlu: 6.981 ± 0.761
3.579AlaPhe: 3.579 ± 0.451
6.318AlaGly: 6.318 ± 0.811
1.193AlaHis: 1.193 ± 0.187
5.081AlaIle: 5.081 ± 0.502
5.214AlaLys: 5.214 ± 0.528
9.146AlaLeu: 9.146 ± 0.994
2.739AlaMet: 2.739 ± 0.332
4.286AlaAsn: 4.286 ± 0.384
4.639AlaPro: 4.639 ± 0.459
5.17AlaGln: 5.17 ± 0.605
3.667AlaArg: 3.667 ± 0.466
4.463AlaSer: 4.463 ± 0.383
6.009AlaThr: 6.009 ± 0.522
6.407AlaVal: 6.407 ± 0.855
1.37AlaTrp: 1.37 ± 0.26
2.872AlaTyr: 2.872 ± 0.3
0.0AlaXaa: 0.0 ± 0.0
Cys
0.442CysAla: 0.442 ± 0.174
0.133CysCys: 0.133 ± 0.077
0.442CysAsp: 0.442 ± 0.151
0.353CysGlu: 0.353 ± 0.111
0.265CysPhe: 0.265 ± 0.102
0.663CysGly: 0.663 ± 0.203
0.177CysHis: 0.177 ± 0.094
0.486CysIle: 0.486 ± 0.145
0.795CysLys: 0.795 ± 0.212
0.707CysLeu: 0.707 ± 0.228
0.088CysMet: 0.088 ± 0.063
0.309CysAsn: 0.309 ± 0.164
0.221CysPro: 0.221 ± 0.112
0.442CysGln: 0.442 ± 0.155
0.398CysArg: 0.398 ± 0.192
0.265CysSer: 0.265 ± 0.137
0.53CysThr: 0.53 ± 0.167
0.221CysVal: 0.221 ± 0.104
0.265CysTrp: 0.265 ± 0.139
0.221CysTyr: 0.221 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
6.318AspAla: 6.318 ± 0.803
0.53AspCys: 0.53 ± 0.155
2.519AspAsp: 2.519 ± 0.342
3.402AspGlu: 3.402 ± 0.389
2.784AspPhe: 2.784 ± 0.363
4.153AspGly: 4.153 ± 0.441
1.016AspHis: 1.016 ± 0.264
3.226AspIle: 3.226 ± 0.409
2.828AspLys: 2.828 ± 0.35
5.081AspLeu: 5.081 ± 0.414
2.253AspMet: 2.253 ± 0.285
1.767AspAsn: 1.767 ± 0.404
3.27AspPro: 3.27 ± 0.352
2.739AspGln: 2.739 ± 0.29
3.005AspArg: 3.005 ± 0.35
2.96AspSer: 2.96 ± 0.368
3.181AspThr: 3.181 ± 0.411
3.623AspVal: 3.623 ± 0.465
0.84AspTrp: 0.84 ± 0.219
2.784AspTyr: 2.784 ± 0.431
0.0AspXaa: 0.0 ± 0.0
Glu
7.379GluAla: 7.379 ± 0.803
0.442GluCys: 0.442 ± 0.14
3.667GluAsp: 3.667 ± 0.388
5.17GluGlu: 5.17 ± 0.598
2.077GluPhe: 2.077 ± 0.283
4.109GluGly: 4.109 ± 0.454
1.281GluHis: 1.281 ± 0.263
3.005GluIle: 3.005 ± 0.35
4.198GluLys: 4.198 ± 0.519
6.274GluLeu: 6.274 ± 0.589
1.812GluMet: 1.812 ± 0.315
2.386GluAsn: 2.386 ± 0.28
2.298GluPro: 2.298 ± 0.43
3.093GluGln: 3.093 ± 0.331
3.226GluArg: 3.226 ± 0.443
2.739GluSer: 2.739 ± 0.362
2.828GluThr: 2.828 ± 0.333
4.065GluVal: 4.065 ± 0.449
0.84GluTrp: 0.84 ± 0.197
2.033GluTyr: 2.033 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
2.916PheAla: 2.916 ± 0.373
0.309PheCys: 0.309 ± 0.106
2.077PheAsp: 2.077 ± 0.324
1.812PheGlu: 1.812 ± 0.293
1.458PhePhe: 1.458 ± 0.259
3.181PheGly: 3.181 ± 0.341
0.574PheHis: 0.574 ± 0.162
2.077PheIle: 2.077 ± 0.24
2.165PheLys: 2.165 ± 0.415
2.828PheLeu: 2.828 ± 0.302
1.502PheMet: 1.502 ± 0.314
1.944PheAsn: 1.944 ± 0.273
1.193PhePro: 1.193 ± 0.204
1.458PheGln: 1.458 ± 0.259
2.253PheArg: 2.253 ± 0.315
2.209PheSer: 2.209 ± 0.394
2.563PheThr: 2.563 ± 0.362
2.298PheVal: 2.298 ± 0.313
0.442PheTrp: 0.442 ± 0.177
1.105PheTyr: 1.105 ± 0.216
0.0PheXaa: 0.0 ± 0.0
Gly
6.142GlyAla: 6.142 ± 0.733
0.486GlyCys: 0.486 ± 0.159
4.374GlyAsp: 4.374 ± 0.453
4.198GlyGlu: 4.198 ± 0.337
2.784GlyPhe: 2.784 ± 0.361
5.302GlyGly: 5.302 ± 1.018
1.016GlyHis: 1.016 ± 0.192
4.065GlyIle: 4.065 ± 0.349
4.993GlyLys: 4.993 ± 0.583
4.949GlyLeu: 4.949 ± 0.563
3.181GlyMet: 3.181 ± 0.359
4.153GlyAsn: 4.153 ± 0.359
1.812GlyPro: 1.812 ± 0.388
3.226GlyGln: 3.226 ± 0.343
3.8GlyArg: 3.8 ± 0.484
4.595GlySer: 4.595 ± 0.576
4.198GlyThr: 4.198 ± 0.515
4.507GlyVal: 4.507 ± 0.41
1.06GlyTrp: 1.06 ± 0.227
2.872GlyTyr: 2.872 ± 0.302
0.0GlyXaa: 0.0 ± 0.0
His
1.546HisAla: 1.546 ± 0.274
0.265HisCys: 0.265 ± 0.103
1.237HisAsp: 1.237 ± 0.232
1.281HisGlu: 1.281 ± 0.284
0.707HisPhe: 0.707 ± 0.131
0.928HisGly: 0.928 ± 0.304
0.619HisHis: 0.619 ± 0.188
1.149HisIle: 1.149 ± 0.291
1.458HisLys: 1.458 ± 0.269
1.944HisLeu: 1.944 ± 0.365
0.574HisMet: 0.574 ± 0.156
0.707HisAsn: 0.707 ± 0.135
0.972HisPro: 0.972 ± 0.239
0.884HisGln: 0.884 ± 0.203
1.37HisArg: 1.37 ± 0.283
0.442HisSer: 0.442 ± 0.155
0.751HisThr: 0.751 ± 0.171
1.149HisVal: 1.149 ± 0.273
0.442HisTrp: 0.442 ± 0.138
0.972HisTyr: 0.972 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
5.258IleAla: 5.258 ± 0.476
0.398IleCys: 0.398 ± 0.152
3.623IleAsp: 3.623 ± 0.48
3.756IleGlu: 3.756 ± 0.463
1.767IlePhe: 1.767 ± 0.335
3.181IleGly: 3.181 ± 0.369
1.237IleHis: 1.237 ± 0.212
2.342IleIle: 2.342 ± 0.395
3.181IleLys: 3.181 ± 0.341
3.844IleLeu: 3.844 ± 0.426
1.502IleMet: 1.502 ± 0.246
2.209IleAsn: 2.209 ± 0.305
2.784IlePro: 2.784 ± 0.353
2.784IleGln: 2.784 ± 0.315
2.43IleArg: 2.43 ± 0.35
2.342IleSer: 2.342 ± 0.25
3.579IleThr: 3.579 ± 0.403
2.828IleVal: 2.828 ± 0.338
0.619IleTrp: 0.619 ± 0.167
1.458IleTyr: 1.458 ± 0.248
0.0IleXaa: 0.0 ± 0.0
Lys
6.009LysAla: 6.009 ± 0.47
0.177LysCys: 0.177 ± 0.098
4.109LysAsp: 4.109 ± 0.467
3.579LysGlu: 3.579 ± 0.495
1.458LysPhe: 1.458 ± 0.239
4.021LysGly: 4.021 ± 0.446
1.502LysHis: 1.502 ± 0.336
2.474LysIle: 2.474 ± 0.267
4.198LysLys: 4.198 ± 0.583
5.523LysLeu: 5.523 ± 0.421
2.077LysMet: 2.077 ± 0.331
2.784LysAsn: 2.784 ± 0.323
2.651LysPro: 2.651 ± 0.467
3.535LysGln: 3.535 ± 0.343
2.916LysArg: 2.916 ± 0.468
3.8LysSer: 3.8 ± 0.451
3.358LysThr: 3.358 ± 0.423
3.712LysVal: 3.712 ± 0.437
0.751LysTrp: 0.751 ± 0.173
1.591LysTyr: 1.591 ± 0.274
0.0LysXaa: 0.0 ± 0.0
Leu
7.732LeuAla: 7.732 ± 0.784
0.884LeuCys: 0.884 ± 0.277
5.302LeuAsp: 5.302 ± 0.313
4.772LeuGlu: 4.772 ± 0.432
3.137LeuPhe: 3.137 ± 0.35
6.274LeuGly: 6.274 ± 0.641
2.209LeuHis: 2.209 ± 0.367
3.491LeuIle: 3.491 ± 0.293
4.86LeuLys: 4.86 ± 0.412
6.893LeuLeu: 6.893 ± 0.822
2.739LeuMet: 2.739 ± 0.277
4.507LeuAsn: 4.507 ± 0.388
4.153LeuPro: 4.153 ± 0.406
3.623LeuGln: 3.623 ± 0.391
4.198LeuArg: 4.198 ± 0.417
4.639LeuSer: 4.639 ± 0.523
6.053LeuThr: 6.053 ± 0.489
5.877LeuVal: 5.877 ± 0.539
1.016LeuTrp: 1.016 ± 0.207
2.386LeuTyr: 2.386 ± 0.278
0.0LeuXaa: 0.0 ± 0.0
Met
2.607MetAla: 2.607 ± 0.358
0.177MetCys: 0.177 ± 0.097
2.077MetAsp: 2.077 ± 0.299
2.298MetGlu: 2.298 ± 0.278
0.884MetPhe: 0.884 ± 0.2
2.519MetGly: 2.519 ± 0.344
0.707MetHis: 0.707 ± 0.176
2.209MetIle: 2.209 ± 0.247
1.723MetLys: 1.723 ± 0.317
2.739MetLeu: 2.739 ± 0.317
0.619MetMet: 0.619 ± 0.19
1.856MetAsn: 1.856 ± 0.279
1.546MetPro: 1.546 ± 0.273
1.9MetGln: 1.9 ± 0.246
1.812MetArg: 1.812 ± 0.257
2.386MetSer: 2.386 ± 0.313
2.209MetThr: 2.209 ± 0.207
2.253MetVal: 2.253 ± 0.336
0.353MetTrp: 0.353 ± 0.159
0.707MetTyr: 0.707 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
3.844AsnAla: 3.844 ± 0.36
0.442AsnCys: 0.442 ± 0.154
2.209AsnAsp: 2.209 ± 0.261
2.386AsnGlu: 2.386 ± 0.297
1.546AsnPhe: 1.546 ± 0.204
3.8AsnGly: 3.8 ± 0.565
0.84AsnHis: 0.84 ± 0.188
1.591AsnIle: 1.591 ± 0.252
2.121AsnLys: 2.121 ± 0.372
4.551AsnLeu: 4.551 ± 0.4
1.679AsnMet: 1.679 ± 0.388
2.474AsnAsn: 2.474 ± 0.405
3.446AsnPro: 3.446 ± 0.524
2.784AsnGln: 2.784 ± 0.29
2.386AsnArg: 2.386 ± 0.309
3.27AsnSer: 3.27 ± 0.419
2.43AsnThr: 2.43 ± 0.373
3.005AsnVal: 3.005 ± 0.38
0.84AsnTrp: 0.84 ± 0.21
1.458AsnTyr: 1.458 ± 0.221
0.0AsnXaa: 0.0 ± 0.0
Pro
3.844ProAla: 3.844 ± 0.382
0.398ProCys: 0.398 ± 0.147
2.739ProAsp: 2.739 ± 0.399
3.623ProGlu: 3.623 ± 0.444
1.812ProPhe: 1.812 ± 0.259
3.535ProGly: 3.535 ± 0.462
0.398ProHis: 0.398 ± 0.109
2.165ProIle: 2.165 ± 0.334
3.049ProLys: 3.049 ± 0.391
3.667ProLeu: 3.667 ± 0.366
1.502ProMet: 1.502 ± 0.215
1.546ProAsn: 1.546 ± 0.366
1.9ProPro: 1.9 ± 0.376
2.033ProGln: 2.033 ± 0.29
1.326ProArg: 1.326 ± 0.283
2.651ProSer: 2.651 ± 0.303
3.977ProThr: 3.977 ± 0.345
4.109ProVal: 4.109 ± 0.436
0.619ProTrp: 0.619 ± 0.21
1.281ProTyr: 1.281 ± 0.296
0.0ProXaa: 0.0 ± 0.0
Gln
6.098GlnAla: 6.098 ± 0.738
0.133GlnCys: 0.133 ± 0.069
2.474GlnAsp: 2.474 ± 0.325
3.579GlnGlu: 3.579 ± 0.437
1.9GlnPhe: 1.9 ± 0.292
3.8GlnGly: 3.8 ± 0.414
0.707GlnHis: 0.707 ± 0.152
2.386GlnIle: 2.386 ± 0.353
2.739GlnLys: 2.739 ± 0.382
3.623GlnLeu: 3.623 ± 0.379
2.209GlnMet: 2.209 ± 0.297
2.209GlnAsn: 2.209 ± 0.304
1.723GlnPro: 1.723 ± 0.217
2.96GlnGln: 2.96 ± 0.39
2.651GlnArg: 2.651 ± 0.356
2.386GlnSer: 2.386 ± 0.426
2.298GlnThr: 2.298 ± 0.215
3.491GlnVal: 3.491 ± 0.284
0.972GlnTrp: 0.972 ± 0.2
1.414GlnTyr: 1.414 ± 0.214
0.0GlnXaa: 0.0 ± 0.0
Arg
4.463ArgAla: 4.463 ± 0.464
0.442ArgCys: 0.442 ± 0.175
2.695ArgAsp: 2.695 ± 0.352
2.916ArgGlu: 2.916 ± 0.275
2.209ArgPhe: 2.209 ± 0.366
2.828ArgGly: 2.828 ± 0.38
0.884ArgHis: 0.884 ± 0.27
2.872ArgIle: 2.872 ± 0.347
2.651ArgLys: 2.651 ± 0.373
5.081ArgLeu: 5.081 ± 0.474
1.944ArgMet: 1.944 ± 0.207
2.298ArgAsn: 2.298 ± 0.313
2.209ArgPro: 2.209 ± 0.361
2.342ArgGln: 2.342 ± 0.368
2.033ArgArg: 2.033 ± 0.491
3.049ArgSer: 3.049 ± 0.356
2.253ArgThr: 2.253 ± 0.256
3.712ArgVal: 3.712 ± 0.375
0.84ArgTrp: 0.84 ± 0.241
1.458ArgTyr: 1.458 ± 0.247
0.0ArgXaa: 0.0 ± 0.0
Ser
4.153SerAla: 4.153 ± 0.456
0.353SerCys: 0.353 ± 0.144
3.446SerAsp: 3.446 ± 0.363
2.739SerGlu: 2.739 ± 0.354
2.209SerPhe: 2.209 ± 0.291
4.242SerGly: 4.242 ± 0.421
1.06SerHis: 1.06 ± 0.23
2.828SerIle: 2.828 ± 0.369
3.27SerLys: 3.27 ± 0.328
4.728SerLeu: 4.728 ± 0.593
1.856SerMet: 1.856 ± 0.28
2.784SerAsn: 2.784 ± 0.402
2.519SerPro: 2.519 ± 0.341
2.209SerGln: 2.209 ± 0.32
3.049SerArg: 3.049 ± 0.425
3.226SerSer: 3.226 ± 0.481
3.623SerThr: 3.623 ± 0.448
3.446SerVal: 3.446 ± 0.441
0.751SerTrp: 0.751 ± 0.19
2.165SerTyr: 2.165 ± 0.312
0.0SerXaa: 0.0 ± 0.0
Thr
5.744ThrAla: 5.744 ± 0.565
0.221ThrCys: 0.221 ± 0.101
3.579ThrAsp: 3.579 ± 0.343
3.491ThrGlu: 3.491 ± 0.316
1.9ThrPhe: 1.9 ± 0.306
5.214ThrGly: 5.214 ± 0.567
0.972ThrHis: 0.972 ± 0.224
3.314ThrIle: 3.314 ± 0.339
3.977ThrLys: 3.977 ± 0.354
4.286ThrLeu: 4.286 ± 0.329
1.767ThrMet: 1.767 ± 0.294
2.916ThrAsn: 2.916 ± 0.356
3.712ThrPro: 3.712 ± 0.374
2.474ThrGln: 2.474 ± 0.344
2.519ThrArg: 2.519 ± 0.321
3.27ThrSer: 3.27 ± 0.261
3.314ThrThr: 3.314 ± 0.445
4.021ThrVal: 4.021 ± 0.441
0.928ThrTrp: 0.928 ± 0.306
1.502ThrTyr: 1.502 ± 0.329
0.0ThrXaa: 0.0 ± 0.0
Val
6.142ValAla: 6.142 ± 0.712
0.751ValCys: 0.751 ± 0.216
3.491ValAsp: 3.491 ± 0.353
3.932ValGlu: 3.932 ± 0.388
1.944ValPhe: 1.944 ± 0.338
4.109ValGly: 4.109 ± 0.577
1.856ValHis: 1.856 ± 0.291
3.402ValIle: 3.402 ± 0.514
3.888ValLys: 3.888 ± 0.365
5.921ValLeu: 5.921 ± 0.482
2.253ValMet: 2.253 ± 0.334
3.446ValAsn: 3.446 ± 0.367
3.27ValPro: 3.27 ± 0.43
3.358ValGln: 3.358 ± 0.347
3.491ValArg: 3.491 ± 0.387
3.844ValSer: 3.844 ± 0.465
3.977ValThr: 3.977 ± 0.46
4.949ValVal: 4.949 ± 0.458
0.84ValTrp: 0.84 ± 0.201
2.033ValTyr: 2.033 ± 0.262
0.0ValXaa: 0.0 ± 0.0
Trp
1.237TrpAla: 1.237 ± 0.178
0.133TrpCys: 0.133 ± 0.069
1.37TrpAsp: 1.37 ± 0.336
0.84TrpGlu: 0.84 ± 0.241
0.707TrpPhe: 0.707 ± 0.195
1.06TrpGly: 1.06 ± 0.372
0.309TrpHis: 0.309 ± 0.116
0.751TrpIle: 0.751 ± 0.2
0.972TrpLys: 0.972 ± 0.137
1.06TrpLeu: 1.06 ± 0.244
0.309TrpMet: 0.309 ± 0.128
0.751TrpAsn: 0.751 ± 0.191
0.398TrpPro: 0.398 ± 0.159
0.53TrpGln: 0.53 ± 0.165
0.884TrpArg: 0.884 ± 0.23
0.795TrpSer: 0.795 ± 0.221
0.53TrpThr: 0.53 ± 0.217
1.105TrpVal: 1.105 ± 0.205
0.398TrpTrp: 0.398 ± 0.218
0.442TrpTyr: 0.442 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.253TyrAla: 2.253 ± 0.282
0.133TyrCys: 0.133 ± 0.085
2.474TyrAsp: 2.474 ± 0.265
1.812TyrGlu: 1.812 ± 0.306
1.237TyrPhe: 1.237 ± 0.228
2.474TyrGly: 2.474 ± 0.401
0.84TyrHis: 0.84 ± 0.227
2.209TyrIle: 2.209 ± 0.357
1.988TyrLys: 1.988 ± 0.343
1.9TyrLeu: 1.9 ± 0.324
0.928TyrMet: 0.928 ± 0.202
1.767TyrAsn: 1.767 ± 0.317
1.458TyrPro: 1.458 ± 0.357
2.209TyrGln: 2.209 ± 0.3
1.767TyrArg: 1.767 ± 0.291
1.326TyrSer: 1.326 ± 0.205
1.458TyrThr: 1.458 ± 0.234
2.121TyrVal: 2.121 ± 0.286
0.398TyrTrp: 0.398 ± 0.124
0.84TyrTyr: 0.84 ± 0.165
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (22633 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski