Acinetobacter phage Ab105-2phi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vieuvirus; unclassified Vieuvirus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0IRH5|A0A0P0IRH5_9CAUD Uncharacterized protein OS=Acinetobacter phage Ab105-2phi OX=1718841 GN=Ab1052phi_15 PE=4 SV=1
MM1 pKa = 7.7SITLNGHH8 pKa = 4.6QLKK11 pKa = 10.73SLLEE15 pKa = 4.06FVNPDD20 pKa = 3.39GEE22 pKa = 4.39NDD24 pKa = 3.55LDD26 pKa = 3.81QLEE29 pKa = 4.54TEE31 pKa = 4.17LTIKK35 pKa = 10.48FFEE38 pKa = 5.54DD39 pKa = 2.98GHH41 pKa = 7.16SGKK44 pKa = 10.68GYY46 pKa = 7.83YY47 pKa = 10.13FWMTEE52 pKa = 4.0YY53 pKa = 10.43PEE55 pKa = 4.23EE56 pKa = 4.3GAMKK60 pKa = 10.55LDD62 pKa = 3.86IEE64 pKa = 4.49WGAEE68 pKa = 3.95GG69 pKa = 4.51

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0IVX0|A0A0P0IVX0_9CAUD DNA helicase OS=Acinetobacter phage Ab105-2phi OX=1718841 GN=Ab1052phi_22 PE=3 SV=1
MM1 pKa = 7.52TNFKK5 pKa = 10.09KK6 pKa = 10.19HH7 pKa = 5.93PDD9 pKa = 3.52GYY11 pKa = 11.13KK12 pKa = 10.38SFLGRR17 pKa = 11.84DD18 pKa = 3.51DD19 pKa = 3.9KK20 pKa = 11.62GLYY23 pKa = 8.82SVRR26 pKa = 11.84IGWQVYY32 pKa = 9.57ASNANGSVLYY42 pKa = 10.14KK43 pKa = 10.87VKK45 pKa = 10.91DD46 pKa = 3.8GVKK49 pKa = 9.28TPLNVFRR56 pKa = 11.84FRR58 pKa = 11.84TSYY61 pKa = 9.84PKK63 pKa = 10.28VWNEE67 pKa = 3.36LTQEE71 pKa = 3.45IDD73 pKa = 3.47FQRR76 pKa = 11.84RR77 pKa = 11.84KK78 pKa = 9.79QLAIKK83 pKa = 10.26LRR85 pKa = 11.84EE86 pKa = 4.24TNIPTYY92 pKa = 10.38DD93 pKa = 3.01RR94 pKa = 11.84KK95 pKa = 10.61AYY97 pKa = 7.93KK98 pKa = 9.58QKK100 pKa = 10.84RR101 pKa = 11.84GFTGSRR107 pKa = 3.24

Molecular weight: 12.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

18288

26

1287

196.6

22.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.437 ± 0.622

0.941 ± 0.119

5.911 ± 0.166

7.054 ± 0.239

3.899 ± 0.207

6.25 ± 0.296

1.69 ± 0.155

6.299 ± 0.191

7.951 ± 0.342

9.017 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.122

5.271 ± 0.187

3.357 ± 0.256

4.686 ± 0.263

4.287 ± 0.174

6.414 ± 0.215

5.583 ± 0.251

6.228 ± 0.196

1.154 ± 0.102

3.199 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski