Streptomyces phage Mischief19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Woodruffvirus; unclassified Woodruffvirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6E4B6|A0A4D6E4B6_9CAUD Uncharacterized protein OS=Streptomyces phage Mischief19 OX=2562344 GN=22 PE=4 SV=1
MM1 pKa = 6.63TTPAFNPLPCDD12 pKa = 3.47AGAGGAADD20 pKa = 4.29DD21 pKa = 4.36VEE23 pKa = 4.72SHH25 pKa = 6.7ILCAVDD31 pKa = 3.78ADD33 pKa = 4.12GVVQASALAVYY44 pKa = 9.07TYY46 pKa = 10.52DD47 pKa = 3.51PQGNPVGAPEE57 pKa = 4.17FVDD60 pKa = 4.06PVTGAPFVLPPGTTLQPCPDD80 pKa = 4.5GGCGGPIQFCQTGTTTGPVQHH101 pKa = 7.37PGRR104 pKa = 11.84TYY106 pKa = 10.96DD107 pKa = 2.79ITLPINPGFAVQSLQIDD124 pKa = 4.09SVSQSAGITWEE135 pKa = 3.73VDD137 pKa = 3.29DD138 pKa = 5.08PFGNTFAADD147 pKa = 3.93LKK149 pKa = 11.24AFIEE153 pKa = 4.3GRR155 pKa = 11.84IAGSTVTVTNPNDD168 pKa = 3.68GQIICGTAAPMQVHH182 pKa = 6.19IEE184 pKa = 4.1CLRR187 pKa = 11.84LDD189 pKa = 3.85QNPPNLIEE197 pKa = 4.07LVYY200 pKa = 10.97NAGQDD205 pKa = 4.35LIINPAYY212 pKa = 10.68NEE214 pKa = 4.18NPPLNPPVSQGNYY227 pKa = 9.34GFHH230 pKa = 7.01LLARR234 pKa = 11.84QDD236 pKa = 3.79DD237 pKa = 4.34PGPFPGNQPSGRR249 pKa = 11.84AVCTDD254 pKa = 2.95VANRR258 pKa = 11.84GWEE261 pKa = 4.1TNDD264 pKa = 3.02RR265 pKa = 11.84GRR267 pKa = 11.84TFEE270 pKa = 3.51IWGNNIVTGEE280 pKa = 4.26NVTPTPRR287 pKa = 11.84GTPVQEE293 pKa = 4.16ITSDD297 pKa = 3.82GPPVAGGRR305 pKa = 11.84PSTIWQTFVAPASGNFIIRR324 pKa = 11.84LVHH327 pKa = 5.81GARR330 pKa = 11.84DD331 pKa = 3.31AGEE334 pKa = 3.98VHH336 pKa = 7.73RR337 pKa = 11.84ITLDD341 pKa = 3.37NGDD344 pKa = 4.42TDD346 pKa = 4.15DD347 pKa = 4.04QQNGTLIDD355 pKa = 3.93DD356 pKa = 3.94TTTPGVVTSSTGGPGPWTQFSQTIPLTAGQTYY388 pKa = 8.41TLALSTNNPVGGARR402 pKa = 11.84GGLFTDD408 pKa = 3.71MRR410 pKa = 11.84AFLDD414 pKa = 3.87RR415 pKa = 11.84PDD417 pKa = 3.87RR418 pKa = 11.84RR419 pKa = 11.84ATAVNDD425 pKa = 3.83DD426 pKa = 4.08DD427 pKa = 4.44TCVVTTTEE435 pKa = 4.1TQTTRR440 pKa = 11.84TCSFWQPQCSGGRR453 pKa = 11.84VAGWVKK459 pKa = 10.68VDD461 pKa = 3.36TGLVLSNAEE470 pKa = 4.27FWAQTPTPQCCDD482 pKa = 3.09STGSGDD488 pKa = 4.0GEE490 pKa = 4.21QSSTLSNLAVSDD502 pKa = 3.95IVCATLGGVQQNAVRR517 pKa = 11.84VVVLDD522 pKa = 3.65PSGGQLSEE530 pKa = 4.42TFLGTDD536 pKa = 4.01GARR539 pKa = 11.84IQPASWSPGACTTEE553 pKa = 3.82RR554 pKa = 11.84TLADD558 pKa = 3.65AVLCDD563 pKa = 4.95FGNLALDD570 pKa = 4.0GTPTSFLRR578 pKa = 11.84KK579 pKa = 9.22YY580 pKa = 8.67VQTWSPTVGTSITEE594 pKa = 3.82VRR596 pKa = 11.84DD597 pKa = 3.81FNLSGNGARR606 pKa = 11.84YY607 pKa = 7.55TPLGLVGDD615 pKa = 4.72CSSLRR620 pKa = 11.84PTDD623 pKa = 3.45TEE625 pKa = 4.2TQLVCDD631 pKa = 4.26VQAPIPGQPPASAQIISRR649 pKa = 11.84ITYY652 pKa = 9.81DD653 pKa = 3.08STGAILSQSFTTLDD667 pKa = 3.7GQPYY671 pKa = 8.41TPGGTILQCPNVDD684 pKa = 3.53TEE686 pKa = 4.93LSVHH690 pKa = 6.57CDD692 pKa = 2.8AGNANHH698 pKa = 6.94RR699 pKa = 11.84FVRR702 pKa = 11.84VYY704 pKa = 10.97NRR706 pKa = 11.84VDD708 pKa = 3.31GVQVNFSTVEE718 pKa = 3.78LDD720 pKa = 3.31GVTPYY725 pKa = 10.24TAVGPLVDD733 pKa = 4.33CAAASVSTDD742 pKa = 4.24PIATTGLCLGDD753 pKa = 3.63GTPIAVVTRR762 pKa = 11.84RR763 pKa = 11.84DD764 pKa = 3.37HH765 pKa = 6.45AAGTIITDD773 pKa = 3.33GWVNLLTGTFSAGAPPVGTTACGSSRR799 pKa = 11.84SIEE802 pKa = 3.96LSGVLCDD809 pKa = 3.68VSGGTTVNGLVLVEE823 pKa = 4.02YY824 pKa = 9.86HH825 pKa = 5.93YY826 pKa = 11.41AADD829 pKa = 4.07GSIEE833 pKa = 4.07STRR836 pKa = 11.84LVNATTGATYY846 pKa = 10.85VPVGTITVCPEE857 pKa = 3.6GTSQPDD863 pKa = 2.92RR864 pKa = 11.84DD865 pKa = 4.01MEE867 pKa = 4.43LLCDD871 pKa = 3.74VAANGTVTPFIRR883 pKa = 11.84DD884 pKa = 3.49YY885 pKa = 11.14QRR887 pKa = 11.84NSTGAITGSTSYY899 pKa = 10.12TLGGAAYY906 pKa = 9.74APTGTVGSCSAKK918 pKa = 10.6DD919 pKa = 3.35SEE921 pKa = 4.69TFILCDD927 pKa = 3.32SAAVPVRR934 pKa = 11.84FLRR937 pKa = 11.84TYY939 pKa = 8.74TYY941 pKa = 10.89KK942 pKa = 10.93ADD944 pKa = 3.47GSVAAFTDD952 pKa = 3.78STFAGAAFTPTGAVGTCATTTTPEE976 pKa = 3.67TDD978 pKa = 3.24VQQEE982 pKa = 4.2ILCDD986 pKa = 3.68ANGTQFIRR994 pKa = 11.84RR995 pKa = 11.84WLFNSATGAVTSTVNLTLAGAAFTPVGAVGLCSNCCPVVVGEE1037 pKa = 4.53GCTNTGSGRR1046 pKa = 11.84YY1047 pKa = 8.22VATRR1051 pKa = 11.84AANGTITLQDD1061 pKa = 3.76SVTGATVTQAQIIACPDD1078 pKa = 3.23QPVTVVAEE1086 pKa = 4.11GRR1088 pKa = 11.84IVADD1092 pKa = 5.03ADD1094 pKa = 4.5TPWSPGNDD1102 pKa = 3.07VIGRR1106 pKa = 11.84LTSVTLTVLSGTANVVDD1123 pKa = 4.46GNGTALTGLPAGYY1136 pKa = 7.14TASWDD1141 pKa = 3.75TQDD1144 pKa = 4.87DD1145 pKa = 4.64HH1146 pKa = 6.72NTLTGPTSIDD1156 pKa = 3.16AVGGSTVVVWTRR1168 pKa = 11.84VV1169 pKa = 3.03

Molecular weight: 120.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6E4L5|A0A4D6E4L5_9CAUD Uncharacterized protein OS=Streptomyces phage Mischief19 OX=2562344 GN=71 PE=4 SV=1
MM1 pKa = 7.58TNTTATKK8 pKa = 8.54VTAAEE13 pKa = 4.21LVAGTIVWGRR23 pKa = 11.84PVRR26 pKa = 11.84EE27 pKa = 4.23GKK29 pKa = 9.96AQSRR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 10.23GLVLGSFGDD44 pKa = 3.72SAVIVWWYY52 pKa = 11.14GEE54 pKa = 4.26GAASGSTTTMAFPRR68 pKa = 11.84EE69 pKa = 4.11LTRR72 pKa = 11.84DD73 pKa = 2.8GDD75 pKa = 4.06MFDD78 pKa = 3.55LSARR82 pKa = 11.84VARR85 pKa = 11.84KK86 pKa = 8.51LAKK89 pKa = 9.63GCYY92 pKa = 8.28GFEE95 pKa = 4.15RR96 pKa = 11.84AASVGWSLTRR106 pKa = 11.84HH107 pKa = 5.9AARR110 pKa = 11.84MASLGASQPVRR121 pKa = 3.42

Molecular weight: 12.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

20485

57

1279

259.3

27.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.41 ± 0.574

1.679 ± 0.203

6.092 ± 0.242

4.926 ± 0.312

2.504 ± 0.183

9.666 ± 0.401

1.816 ± 0.17

3.691 ± 0.242

3.056 ± 0.302

8.352 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.884 ± 0.14

3.373 ± 0.241

5.297 ± 0.271

3.061 ± 0.141

6.375 ± 0.336

5.648 ± 0.25

7.508 ± 0.448

8.05 ± 0.162

1.425 ± 0.13

2.187 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski