Amino acid dipepetide frequency for Streptomyces phage Mischief19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.335AlaAla: 23.335 ± 1.954
1.465AlaCys: 1.465 ± 0.353
10.252AlaAsp: 10.252 ± 0.944
8.006AlaGlu: 8.006 ± 0.829
3.613AlaPhe: 3.613 ± 0.55
10.35AlaGly: 10.35 ± 0.753
1.806AlaHis: 1.806 ± 0.295
4.296AlaIle: 4.296 ± 0.759
5.028AlaLys: 5.028 ± 0.829
11.521AlaLeu: 11.521 ± 1.403
2.734AlaMet: 2.734 ± 0.362
3.368AlaAsn: 3.368 ± 0.373
6.151AlaPro: 6.151 ± 0.746
4.198AlaGln: 4.198 ± 0.488
7.713AlaArg: 7.713 ± 0.647
7.176AlaSer: 7.176 ± 0.649
8.983AlaThr: 8.983 ± 0.822
10.252AlaVal: 10.252 ± 0.794
1.416AlaTrp: 1.416 ± 0.236
2.441AlaTyr: 2.441 ± 0.338
0.0AlaXaa: 0.0 ± 0.0
Cys
2.05CysAla: 2.05 ± 0.394
0.439CysCys: 0.439 ± 0.173
1.709CysAsp: 1.709 ± 0.415
0.586CysGlu: 0.586 ± 0.161
0.342CysPhe: 0.342 ± 0.134
1.953CysGly: 1.953 ± 0.426
0.635CysHis: 0.635 ± 0.174
0.439CysIle: 0.439 ± 0.146
0.537CysLys: 0.537 ± 0.198
1.025CysLeu: 1.025 ± 0.251
0.195CysMet: 0.195 ± 0.136
0.635CysAsn: 0.635 ± 0.21
1.416CysPro: 1.416 ± 0.39
0.391CysGln: 0.391 ± 0.116
1.074CysArg: 1.074 ± 0.248
1.123CysSer: 1.123 ± 0.291
0.928CysThr: 0.928 ± 0.237
0.781CysVal: 0.781 ± 0.265
0.195CysTrp: 0.195 ± 0.098
0.342CysTyr: 0.342 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
8.153AspAla: 8.153 ± 0.579
1.465AspCys: 1.465 ± 0.367
4.003AspAsp: 4.003 ± 0.498
4.247AspGlu: 4.247 ± 0.512
0.976AspPhe: 0.976 ± 0.198
8.25AspGly: 8.25 ± 0.751
0.781AspHis: 0.781 ± 0.227
1.465AspIle: 1.465 ± 0.291
1.806AspLys: 1.806 ± 0.384
4.882AspLeu: 4.882 ± 0.493
1.855AspMet: 1.855 ± 0.288
1.611AspAsn: 1.611 ± 0.373
2.831AspPro: 2.831 ± 0.475
1.318AspGln: 1.318 ± 0.236
4.296AspArg: 4.296 ± 0.438
2.587AspSer: 2.587 ± 0.415
3.027AspThr: 3.027 ± 0.469
5.224AspVal: 5.224 ± 0.503
0.781AspTrp: 0.781 ± 0.259
1.367AspTyr: 1.367 ± 0.259
0.0AspXaa: 0.0 ± 0.0
Glu
8.299GluAla: 8.299 ± 0.89
0.781GluCys: 0.781 ± 0.208
1.367GluAsp: 1.367 ± 0.31
1.904GluGlu: 1.904 ± 0.369
1.66GluPhe: 1.66 ± 0.256
4.54GluGly: 4.54 ± 0.526
0.928GluHis: 0.928 ± 0.241
2.05GluIle: 2.05 ± 0.344
0.098GluLys: 0.098 ± 0.062
5.419GluLeu: 5.419 ± 0.651
0.781GluMet: 0.781 ± 0.209
1.318GluAsn: 1.318 ± 0.207
2.148GluPro: 2.148 ± 0.378
2.05GluGln: 2.05 ± 0.379
4.491GluArg: 4.491 ± 0.574
1.806GluSer: 1.806 ± 0.368
3.222GluThr: 3.222 ± 0.501
4.296GluVal: 4.296 ± 0.499
1.172GluTrp: 1.172 ± 0.26
0.928GluTyr: 0.928 ± 0.236
0.0GluXaa: 0.0 ± 0.0
Phe
2.294PheAla: 2.294 ± 0.29
0.342PheCys: 0.342 ± 0.124
2.148PheAsp: 2.148 ± 0.32
1.416PheGlu: 1.416 ± 0.288
0.879PhePhe: 0.879 ± 0.22
2.197PheGly: 2.197 ± 0.447
0.293PheHis: 0.293 ± 0.114
0.928PheIle: 0.928 ± 0.294
1.025PheLys: 1.025 ± 0.242
2.197PheLeu: 2.197 ± 0.346
0.293PheMet: 0.293 ± 0.117
1.269PheAsn: 1.269 ± 0.283
1.123PhePro: 1.123 ± 0.233
0.732PheGln: 0.732 ± 0.195
0.683PheArg: 0.683 ± 0.194
1.757PheSer: 1.757 ± 0.341
2.685PheThr: 2.685 ± 0.371
1.953PheVal: 1.953 ± 0.374
0.195PheTrp: 0.195 ± 0.11
0.635PheTyr: 0.635 ± 0.183
0.0PheXaa: 0.0 ± 0.0
Gly
11.228GlyAla: 11.228 ± 0.982
2.197GlyCys: 2.197 ± 0.503
4.931GlyAsp: 4.931 ± 0.588
4.833GlyGlu: 4.833 ± 0.457
2.636GlyPhe: 2.636 ± 0.379
9.52GlyGly: 9.52 ± 1.549
1.709GlyHis: 1.709 ± 0.346
3.759GlyIle: 3.759 ± 0.664
3.661GlyLys: 3.661 ± 0.558
7.176GlyLeu: 7.176 ± 0.766
2.148GlyMet: 2.148 ± 0.338
4.003GlyAsn: 4.003 ± 0.547
4.833GlyPro: 4.833 ± 0.503
3.613GlyGln: 3.613 ± 0.381
5.858GlyArg: 5.858 ± 0.519
6.151GlySer: 6.151 ± 0.539
7.225GlyThr: 7.225 ± 0.94
6.786GlyVal: 6.786 ± 0.667
1.611GlyTrp: 1.611 ± 0.311
2.783GlyTyr: 2.783 ± 0.423
0.0GlyXaa: 0.0 ± 0.0
His
2.294HisAla: 2.294 ± 0.36
0.342HisCys: 0.342 ± 0.167
1.269HisAsp: 1.269 ± 0.288
1.172HisGlu: 1.172 ± 0.26
0.244HisPhe: 0.244 ± 0.097
1.172HisGly: 1.172 ± 0.3
0.488HisHis: 0.488 ± 0.138
0.732HisIle: 0.732 ± 0.204
0.293HisLys: 0.293 ± 0.139
1.611HisLeu: 1.611 ± 0.307
0.391HisMet: 0.391 ± 0.146
0.537HisAsn: 0.537 ± 0.175
1.025HisPro: 1.025 ± 0.289
0.342HisGln: 0.342 ± 0.15
1.269HisArg: 1.269 ± 0.262
0.879HisSer: 0.879 ± 0.205
1.416HisThr: 1.416 ± 0.296
1.416HisVal: 1.416 ± 0.324
0.488HisTrp: 0.488 ± 0.15
0.781HisTyr: 0.781 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
4.296IleAla: 4.296 ± 0.36
0.488IleCys: 0.488 ± 0.113
3.173IleAsp: 3.173 ± 0.378
2.294IleGlu: 2.294 ± 0.291
0.391IlePhe: 0.391 ± 0.146
3.905IleGly: 3.905 ± 0.665
0.488IleHis: 0.488 ± 0.145
1.562IleIle: 1.562 ± 0.374
1.611IleLys: 1.611 ± 0.369
2.343IleLeu: 2.343 ± 0.4
0.488IleMet: 0.488 ± 0.144
1.855IleAsn: 1.855 ± 0.292
1.22IlePro: 1.22 ± 0.247
0.683IleGln: 0.683 ± 0.185
1.757IleArg: 1.757 ± 0.315
2.148IleSer: 2.148 ± 0.419
4.052IleThr: 4.052 ± 0.623
3.076IleVal: 3.076 ± 0.367
0.439IleTrp: 0.439 ± 0.14
0.635IleTyr: 0.635 ± 0.219
0.0IleXaa: 0.0 ± 0.0
Lys
5.175LysAla: 5.175 ± 0.64
0.293LysCys: 0.293 ± 0.198
1.318LysAsp: 1.318 ± 0.311
1.22LysGlu: 1.22 ± 0.233
0.244LysPhe: 0.244 ± 0.103
3.564LysGly: 3.564 ± 0.463
0.879LysHis: 0.879 ± 0.195
1.318LysIle: 1.318 ± 0.408
0.391LysLys: 0.391 ± 0.194
2.929LysLeu: 2.929 ± 0.514
0.928LysMet: 0.928 ± 0.213
0.586LysAsn: 0.586 ± 0.152
1.953LysPro: 1.953 ± 0.437
0.928LysGln: 0.928 ± 0.217
2.197LysArg: 2.197 ± 0.412
1.416LysSer: 1.416 ± 0.304
2.294LysThr: 2.294 ± 0.43
2.246LysVal: 2.246 ± 0.306
0.293LysTrp: 0.293 ± 0.097
0.391LysTyr: 0.391 ± 0.137
0.0LysXaa: 0.0 ± 0.0
Leu
10.935LeuAla: 10.935 ± 1.054
1.172LeuCys: 1.172 ± 0.368
5.272LeuAsp: 5.272 ± 0.463
2.685LeuGlu: 2.685 ± 0.405
1.513LeuPhe: 1.513 ± 0.289
7.811LeuGly: 7.811 ± 0.657
1.22LeuHis: 1.22 ± 0.322
3.124LeuIle: 3.124 ± 0.435
3.515LeuLys: 3.515 ± 0.48
6.444LeuLeu: 6.444 ± 0.73
1.123LeuMet: 1.123 ± 0.236
2.929LeuAsn: 2.929 ± 0.437
5.468LeuPro: 5.468 ± 0.533
1.709LeuGln: 1.709 ± 0.308
6.444LeuArg: 6.444 ± 0.73
5.517LeuSer: 5.517 ± 0.668
7.713LeuThr: 7.713 ± 0.597
6.835LeuVal: 6.835 ± 0.487
0.683LeuTrp: 0.683 ± 0.181
1.416LeuTyr: 1.416 ± 0.282
0.0LeuXaa: 0.0 ± 0.0
Met
2.831MetAla: 2.831 ± 0.373
0.244MetCys: 0.244 ± 0.12
0.195MetAsp: 0.195 ± 0.09
0.391MetGlu: 0.391 ± 0.109
0.439MetPhe: 0.439 ± 0.154
1.318MetGly: 1.318 ± 0.265
0.586MetHis: 0.586 ± 0.167
1.172MetIle: 1.172 ± 0.224
0.195MetLys: 0.195 ± 0.1
1.709MetLeu: 1.709 ± 0.276
0.244MetMet: 0.244 ± 0.131
0.781MetAsn: 0.781 ± 0.182
1.269MetPro: 1.269 ± 0.254
0.83MetGln: 0.83 ± 0.208
1.513MetArg: 1.513 ± 0.284
1.757MetSer: 1.757 ± 0.348
1.513MetThr: 1.513 ± 0.28
1.123MetVal: 1.123 ± 0.347
0.439MetTrp: 0.439 ± 0.17
0.244MetTyr: 0.244 ± 0.107
0.0MetXaa: 0.0 ± 0.0
Asn
4.052AsnAla: 4.052 ± 0.468
0.635AsnCys: 0.635 ± 0.205
1.66AsnAsp: 1.66 ± 0.355
1.513AsnGlu: 1.513 ± 0.341
0.781AsnPhe: 0.781 ± 0.185
4.589AsnGly: 4.589 ± 0.575
0.683AsnHis: 0.683 ± 0.196
1.562AsnIle: 1.562 ± 0.319
0.83AsnLys: 0.83 ± 0.25
3.076AsnLeu: 3.076 ± 0.441
0.293AsnMet: 0.293 ± 0.117
0.976AsnAsn: 0.976 ± 0.212
2.734AsnPro: 2.734 ± 0.531
0.586AsnGln: 0.586 ± 0.195
2.49AsnArg: 2.49 ± 0.445
1.465AsnSer: 1.465 ± 0.227
1.904AsnThr: 1.904 ± 0.385
3.076AsnVal: 3.076 ± 0.433
0.195AsnTrp: 0.195 ± 0.082
0.635AsnTyr: 0.635 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
6.932ProAla: 6.932 ± 0.751
1.123ProCys: 1.123 ± 0.306
3.368ProAsp: 3.368 ± 0.374
2.587ProGlu: 2.587 ± 0.352
1.367ProPhe: 1.367 ± 0.304
5.419ProGly: 5.419 ± 0.564
1.416ProHis: 1.416 ± 0.339
1.709ProIle: 1.709 ± 0.376
1.172ProLys: 1.172 ± 0.317
4.101ProLeu: 4.101 ± 0.488
1.074ProMet: 1.074 ± 0.235
1.074ProAsn: 1.074 ± 0.266
2.685ProPro: 2.685 ± 0.499
2.099ProGln: 2.099 ± 0.355
2.49ProArg: 2.49 ± 0.425
3.466ProSer: 3.466 ± 0.461
3.71ProThr: 3.71 ± 0.493
5.175ProVal: 5.175 ± 0.583
0.879ProTrp: 0.879 ± 0.21
1.123ProTyr: 1.123 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
2.392GlnAla: 2.392 ± 0.285
0.781GlnCys: 0.781 ± 0.256
1.22GlnAsp: 1.22 ± 0.237
0.976GlnGlu: 0.976 ± 0.221
1.416GlnPhe: 1.416 ± 0.243
2.294GlnGly: 2.294 ± 0.308
0.879GlnHis: 0.879 ± 0.184
1.22GlnIle: 1.22 ± 0.24
0.488GlnLys: 0.488 ± 0.156
3.271GlnLeu: 3.271 ± 0.458
0.488GlnMet: 0.488 ± 0.153
1.269GlnAsn: 1.269 ± 0.231
2.734GlnPro: 2.734 ± 0.395
1.465GlnGln: 1.465 ± 0.273
2.441GlnArg: 2.441 ± 0.397
1.562GlnSer: 1.562 ± 0.289
2.88GlnThr: 2.88 ± 0.376
1.855GlnVal: 1.855 ± 0.302
0.586GlnTrp: 0.586 ± 0.182
0.391GlnTyr: 0.391 ± 0.201
0.0GlnXaa: 0.0 ± 0.0
Arg
7.567ArgAla: 7.567 ± 0.598
1.318ArgCys: 1.318 ± 0.288
3.417ArgAsp: 3.417 ± 0.427
3.368ArgGlu: 3.368 ± 0.52
1.562ArgPhe: 1.562 ± 0.244
4.979ArgGly: 4.979 ± 0.603
1.562ArgHis: 1.562 ± 0.372
2.636ArgIle: 2.636 ± 0.363
2.685ArgLys: 2.685 ± 0.406
5.321ArgLeu: 5.321 ± 0.518
1.855ArgMet: 1.855 ± 0.366
2.197ArgAsn: 2.197 ± 0.38
3.271ArgPro: 3.271 ± 0.446
1.806ArgGln: 1.806 ± 0.377
5.126ArgArg: 5.126 ± 0.693
2.734ArgSer: 2.734 ± 0.366
3.71ArgThr: 3.71 ± 0.516
5.712ArgVal: 5.712 ± 0.524
1.025ArgTrp: 1.025 ± 0.239
1.904ArgTyr: 1.904 ± 0.363
0.0ArgXaa: 0.0 ± 0.0
Ser
7.323SerAla: 7.323 ± 0.786
1.074SerCys: 1.074 ± 0.294
2.929SerAsp: 2.929 ± 0.353
2.343SerGlu: 2.343 ± 0.324
2.148SerPhe: 2.148 ± 0.346
6.298SerGly: 6.298 ± 0.715
0.879SerHis: 0.879 ± 0.236
2.197SerIle: 2.197 ± 0.278
1.66SerLys: 1.66 ± 0.321
5.077SerLeu: 5.077 ± 0.469
1.074SerMet: 1.074 ± 0.22
2.246SerAsn: 2.246 ± 0.304
2.392SerPro: 2.392 ± 0.35
1.855SerGln: 1.855 ± 0.333
2.783SerArg: 2.783 ± 0.379
2.441SerSer: 2.441 ± 0.355
5.419SerThr: 5.419 ± 0.688
4.345SerVal: 4.345 ± 0.353
0.781SerTrp: 0.781 ± 0.154
1.22SerTyr: 1.22 ± 0.229
0.0SerXaa: 0.0 ± 0.0
Thr
10.398ThrAla: 10.398 ± 0.735
1.025ThrCys: 1.025 ± 0.293
4.052ThrAsp: 4.052 ± 0.633
4.296ThrGlu: 4.296 ± 0.482
2.197ThrPhe: 2.197 ± 0.387
8.69ThrGly: 8.69 ± 0.918
0.683ThrHis: 0.683 ± 0.164
3.027ThrIle: 3.027 ± 0.482
1.904ThrLys: 1.904 ± 0.264
5.907ThrLeu: 5.907 ± 0.678
1.172ThrMet: 1.172 ± 0.233
2.197ThrAsn: 2.197 ± 0.31
4.54ThrPro: 4.54 ± 0.678
1.806ThrGln: 1.806 ± 0.354
4.003ThrArg: 4.003 ± 0.501
3.515ThrSer: 3.515 ± 0.417
5.419ThrThr: 5.419 ± 0.864
7.469ThrVal: 7.469 ± 0.607
0.732ThrTrp: 0.732 ± 0.206
2.05ThrTyr: 2.05 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
11.424ValAla: 11.424 ± 0.958
1.074ValCys: 1.074 ± 0.206
5.907ValAsp: 5.907 ± 0.403
4.003ValGlu: 4.003 ± 0.413
2.002ValPhe: 2.002 ± 0.326
5.858ValGly: 5.858 ± 0.609
1.513ValHis: 1.513 ± 0.358
2.929ValIle: 2.929 ± 0.426
2.636ValLys: 2.636 ± 0.403
6.249ValLeu: 6.249 ± 0.492
1.172ValMet: 1.172 ± 0.271
3.71ValAsn: 3.71 ± 0.491
3.759ValPro: 3.759 ± 0.444
3.076ValGln: 3.076 ± 0.447
4.394ValArg: 4.394 ± 0.595
5.712ValSer: 5.712 ± 0.726
6.102ValThr: 6.102 ± 0.723
5.761ValVal: 5.761 ± 0.473
1.513ValTrp: 1.513 ± 0.354
1.709ValTyr: 1.709 ± 0.283
0.0ValXaa: 0.0 ± 0.0
Trp
1.172TrpAla: 1.172 ± 0.24
0.195TrpCys: 0.195 ± 0.102
0.732TrpAsp: 0.732 ± 0.174
0.537TrpGlu: 0.537 ± 0.151
0.439TrpPhe: 0.439 ± 0.151
0.83TrpGly: 0.83 ± 0.182
0.244TrpHis: 0.244 ± 0.11
0.195TrpIle: 0.195 ± 0.093
0.391TrpLys: 0.391 ± 0.151
1.611TrpLeu: 1.611 ± 0.263
0.049TrpMet: 0.049 ± 0.047
0.342TrpAsn: 0.342 ± 0.148
0.683TrpPro: 0.683 ± 0.152
0.879TrpGln: 0.879 ± 0.175
0.976TrpArg: 0.976 ± 0.272
2.002TrpSer: 2.002 ± 0.33
1.074TrpThr: 1.074 ± 0.185
1.123TrpVal: 1.123 ± 0.24
0.342TrpTrp: 0.342 ± 0.11
0.439TrpTyr: 0.439 ± 0.143
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.953TyrAla: 1.953 ± 0.31
0.342TyrCys: 0.342 ± 0.137
2.002TyrAsp: 2.002 ± 0.284
0.879TyrGlu: 0.879 ± 0.217
0.195TyrPhe: 0.195 ± 0.096
3.124TyrGly: 3.124 ± 0.535
0.488TyrHis: 0.488 ± 0.12
0.586TyrIle: 0.586 ± 0.165
0.635TyrLys: 0.635 ± 0.153
1.66TyrLeu: 1.66 ± 0.351
0.439TyrMet: 0.439 ± 0.16
0.83TyrAsn: 0.83 ± 0.205
0.732TyrPro: 0.732 ± 0.216
0.244TyrGln: 0.244 ± 0.088
1.757TyrArg: 1.757 ± 0.297
1.269TyrSer: 1.269 ± 0.267
1.806TyrThr: 1.806 ± 0.373
2.002TyrVal: 2.002 ± 0.307
0.488TyrTrp: 0.488 ± 0.15
0.439TyrTyr: 0.439 ± 0.136
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (20485 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski