Clostridium sp. DMHC 10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2713 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L8EWQ0|A0A0L8EWQ0_9CLOT LacI family transcriptional regulator OS=Clostridium sp. DMHC 10 OX=747377 GN=AGR56_17715 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 4.51EE3 pKa = 4.65KK4 pKa = 11.37EE5 pKa = 4.37NLNTCDD11 pKa = 4.23CGCCGEE17 pKa = 4.16EE18 pKa = 4.29HH19 pKa = 6.08EE20 pKa = 5.22HH21 pKa = 6.67EE22 pKa = 4.46EE23 pKa = 4.06HH24 pKa = 7.35HH25 pKa = 6.87HH26 pKa = 7.29DD27 pKa = 4.32CDD29 pKa = 4.29CGCDD33 pKa = 3.78CGEE36 pKa = 4.66HH37 pKa = 5.97EE38 pKa = 4.5PMMVEE43 pKa = 5.62LEE45 pKa = 4.31DD46 pKa = 3.98EE47 pKa = 4.46NGNSISCEE55 pKa = 3.78IVDD58 pKa = 3.88GFVYY62 pKa = 10.27EE63 pKa = 4.43DD64 pKa = 3.62KK65 pKa = 11.3EE66 pKa = 4.31FVLVQNPQNDD76 pKa = 3.58SVYY79 pKa = 9.52LFKK82 pKa = 11.33VEE84 pKa = 4.15GEE86 pKa = 4.1EE87 pKa = 4.57GEE89 pKa = 5.29LVIPDD94 pKa = 3.97DD95 pKa = 4.56NEE97 pKa = 3.56FEE99 pKa = 4.31AASKK103 pKa = 10.27YY104 pKa = 10.28YY105 pKa = 10.64EE106 pKa = 4.56SIVSKK111 pKa = 11.09

Molecular weight: 12.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L8F3L3|A0A0L8F3L3_9CLOT UPF0122 protein AGR56_01295 OS=Clostridium sp. DMHC 10 OX=747377 GN=AGR56_01295 PE=3 SV=1
MM1 pKa = 7.48SRR3 pKa = 11.84KK4 pKa = 9.55CDD6 pKa = 3.07ICGKK10 pKa = 10.13GVVAGVQYY18 pKa = 11.25SHH20 pKa = 6.63AHH22 pKa = 4.91NQSKK26 pKa = 8.11RR27 pKa = 11.84TWLPNIRR34 pKa = 11.84TVKK37 pKa = 10.41AVVNGTPKK45 pKa = 10.02TIHH48 pKa = 5.45VCTRR52 pKa = 11.84CLRR55 pKa = 11.84SGKK58 pKa = 7.37VQRR61 pKa = 11.84AII63 pKa = 3.7

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2713

0

2713

745997

37

2860

275.0

31.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.949 ± 0.056

1.254 ± 0.019

5.417 ± 0.036

6.916 ± 0.061

4.5 ± 0.034

6.343 ± 0.048

1.366 ± 0.018

9.709 ± 0.056

9.4 ± 0.055

8.74 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.022

6.295 ± 0.047

2.861 ± 0.028

2.366 ± 0.028

3.274 ± 0.03

6.569 ± 0.053

4.887 ± 0.042

6.614 ± 0.043

0.717 ± 0.017

4.129 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski