Amino acid dipepetide frequency for Clostridium sp. DMHC 10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.948AlaAla: 4.948 ± 0.103	0.716AlaCys: 0.716 ± 0.031	2.782AlaAsp: 2.782 ± 0.063	3.578AlaGlu: 3.578 ± 0.079	2.838AlaPhe: 2.838 ± 0.072	3.916AlaGly: 3.916 ± 0.092	0.854AlaHis: 0.854 ± 0.035	5.586AlaIle: 5.586 ± 0.089	4.942AlaLys: 4.942 ± 0.083	5.954AlaLeu: 5.954 ± 0.098	1.611AlaMet: 1.611 ± 0.045	2.893AlaAsn: 2.893 ± 0.06	1.521AlaPro: 1.521 ± 0.046	1.468AlaGln: 1.468 ± 0.05	1.949AlaArg: 1.949 ± 0.047	3.906AlaSer: 3.906 ± 0.076	2.448AlaThr: 2.448 ± 0.066	4.802AlaVal: 4.802 ± 0.109	0.395AlaTrp: 0.395 ± 0.023	2.385AlaTyr: 2.385 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.708CysAla: 0.708 ± 0.031	0.202CysCys: 0.202 ± 0.018	0.737CysAsp: 0.737 ± 0.031	0.76CysGlu: 0.76 ± 0.035	0.605CysPhe: 0.605 ± 0.033	1.173CysGly: 1.173 ± 0.045	0.198CysHis: 0.198 ± 0.016	1.204CysIle: 1.204 ± 0.042	1.039CysLys: 1.039 ± 0.038	0.902CysLeu: 0.902 ± 0.035	0.34CysMet: 0.34 ± 0.019	0.696CysAsn: 0.696 ± 0.031	0.5CysPro: 0.5 ± 0.028	0.206CysGln: 0.206 ± 0.015	0.413CysArg: 0.413 ± 0.025	0.95CysSer: 0.95 ± 0.035	0.662CysThr: 0.662 ± 0.032	0.733CysVal: 0.733 ± 0.031	0.071CysTrp: 0.071 ± 0.012	0.441CysTyr: 0.441 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	2.849AspAla: 2.849 ± 0.063	0.601AspCys: 0.601 ± 0.03	2.933AspAsp: 2.933 ± 0.071	4.53AspGlu: 4.53 ± 0.089	2.757AspPhe: 2.757 ± 0.052	3.484AspGly: 3.484 ± 0.073	0.555AspHis: 0.555 ± 0.029	5.979AspIle: 5.979 ± 0.089	5.483AspLys: 5.483 ± 0.099	4.355AspLeu: 4.355 ± 0.069	1.513AspMet: 1.513 ± 0.048	3.245AspAsn: 3.245 ± 0.073	1.381AspPro: 1.381 ± 0.044	0.728AspGln: 0.728 ± 0.031	1.714AspArg: 1.714 ± 0.057	3.083AspSer: 3.083 ± 0.067	2.442AspThr: 2.442 ± 0.059	3.614AspVal: 3.614 ± 0.072	0.386AspTrp: 0.386 ± 0.022	2.538AspTyr: 2.538 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	4.375GluAla: 4.375 ± 0.079	0.721GluCys: 0.721 ± 0.036	3.701GluAsp: 3.701 ± 0.072	5.788GluGlu: 5.788 ± 0.112	2.925GluPhe: 2.925 ± 0.069	3.713GluGly: 3.713 ± 0.083	0.94GluHis: 0.94 ± 0.037	6.763GluIle: 6.763 ± 0.121	7.645GluLys: 7.645 ± 0.124	6.332GluLeu: 6.332 ± 0.092	1.808GluMet: 1.808 ± 0.056	5.102GluAsn: 5.102 ± 0.095	1.438GluPro: 1.438 ± 0.043	1.657GluGln: 1.657 ± 0.054	2.425GluArg: 2.425 ± 0.062	3.225GluSer: 3.225 ± 0.06	2.838GluThr: 2.838 ± 0.063	4.59GluVal: 4.59 ± 0.096	0.436GluTrp: 0.436 ± 0.024	2.741GluTyr: 2.741 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	2.461PheAla: 2.461 ± 0.066	0.61PheCys: 0.61 ± 0.035	2.566PheAsp: 2.566 ± 0.065	2.641PheGlu: 2.641 ± 0.058	2.055PhePhe: 2.055 ± 0.055	3.023PheGly: 3.023 ± 0.066	0.599PheHis: 0.599 ± 0.03	4.578PheIle: 4.578 ± 0.095	3.96PheLys: 3.96 ± 0.089	3.908PheLeu: 3.908 ± 0.084	1.322PheMet: 1.322 ± 0.048	2.871PheAsn: 2.871 ± 0.065	1.275PhePro: 1.275 ± 0.039	1.064PheGln: 1.064 ± 0.039	1.263PheArg: 1.263 ± 0.045	3.386PheSer: 3.386 ± 0.07	2.332PheThr: 2.332 ± 0.056	2.908PheVal: 2.908 ± 0.068	0.335PheTrp: 0.335 ± 0.023	1.842PheTyr: 1.842 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	4.071GlyAla: 4.071 ± 0.089	0.903GlyCys: 0.903 ± 0.04	3.11GlyAsp: 3.11 ± 0.067	4.027GlyGlu: 4.027 ± 0.081	3.193GlyPhe: 3.193 ± 0.067	4.035GlyGly: 4.035 ± 0.104	0.941GlyHis: 0.941 ± 0.035	6.847GlyIle: 6.847 ± 0.102	5.849GlyLys: 5.849 ± 0.086	5.135GlyLeu: 5.135 ± 0.103	1.753GlyMet: 1.753 ± 0.047	3.351GlyAsn: 3.351 ± 0.075	1.251GlyPro: 1.251 ± 0.044	1.496GlyGln: 1.496 ± 0.05	2.196GlyArg: 2.196 ± 0.053	3.708GlySer: 3.708 ± 0.076	3.59GlyThr: 3.59 ± 0.08	4.543GlyVal: 4.543 ± 0.081	0.525GlyTrp: 0.525 ± 0.031	2.904GlyTyr: 2.904 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	0.709HisAla: 0.709 ± 0.026	0.181HisCys: 0.181 ± 0.015	0.735HisAsp: 0.735 ± 0.031	0.917HisGlu: 0.917 ± 0.036	0.638HisPhe: 0.638 ± 0.027	1.047HisGly: 1.047 ± 0.038	0.312HisHis: 0.312 ± 0.028	1.279HisIle: 1.279 ± 0.046	1.082HisLys: 1.082 ± 0.04	1.172HisLeu: 1.172 ± 0.042	0.355HisMet: 0.355 ± 0.02	0.788HisAsn: 0.788 ± 0.03	0.584HisPro: 0.584 ± 0.03	0.316HisGln: 0.316 ± 0.022	0.465HisArg: 0.465 ± 0.027	0.877HisSer: 0.877 ± 0.032	0.693HisThr: 0.693 ± 0.03	0.827HisVal: 0.827 ± 0.035	0.123HisTrp: 0.123 ± 0.015	0.559HisTyr: 0.559 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.922IleAla: 5.922 ± 0.097	1.328IleCys: 1.328 ± 0.044	5.623IleAsp: 5.623 ± 0.09	6.509IleGlu: 6.509 ± 0.103	4.312IlePhe: 4.312 ± 0.085	6.117IleGly: 6.117 ± 0.097	1.248IleHis: 1.248 ± 0.043	9.122IleIle: 9.122 ± 0.138	9.032IleLys: 9.032 ± 0.117	8.658IleLeu: 8.658 ± 0.127	2.442IleMet: 2.442 ± 0.052	6.063IleAsn: 6.063 ± 0.1	3.224IlePro: 3.224 ± 0.061	2.106IleGln: 2.106 ± 0.049	3.02IleArg: 3.02 ± 0.066	7.058IleSer: 7.058 ± 0.108	4.975IleThr: 4.975 ± 0.089	6.237IleVal: 6.237 ± 0.104	0.58IleTrp: 0.58 ± 0.028	3.509IleTyr: 3.509 ± 0.067	0.0IleXaa: 0.0 ± 0.0
Lys	5.558LysAla: 5.558 ± 0.085	0.976LysCys: 0.976 ± 0.038	5.586LysAsp: 5.586 ± 0.1	8.07LysGlu: 8.07 ± 0.128	3.48LysPhe: 3.48 ± 0.066	5.146LysGly: 5.146 ± 0.09	1.252LysHis: 1.252 ± 0.045	8.531LysIle: 8.531 ± 0.112	8.692LysLys: 8.692 ± 0.119	8.517LysLeu: 8.517 ± 0.109	2.621LysMet: 2.621 ± 0.057	6.925LysAsn: 6.925 ± 0.103	2.294LysPro: 2.294 ± 0.062	2.334LysGln: 2.334 ± 0.057	3.139LysArg: 3.139 ± 0.068	5.559LysSer: 5.559 ± 0.105	4.198LysThr: 4.198 ± 0.075	6.201LysVal: 6.201 ± 0.087	0.696LysTrp: 0.696 ± 0.035	4.231LysTyr: 4.231 ± 0.07	0.0LysXaa: 0.0 ± 0.0
Leu	4.953LeuAla: 4.953 ± 0.083	1.236LeuCys: 1.236 ± 0.033	4.727LeuAsp: 4.727 ± 0.087	5.357LeuGlu: 5.357 ± 0.09	3.673LeuPhe: 3.673 ± 0.08	5.645LeuGly: 5.645 ± 0.092	1.086LeuHis: 1.086 ± 0.039	7.96LeuIle: 7.96 ± 0.121	9.165LeuLys: 9.165 ± 0.115	7.196LeuLeu: 7.196 ± 0.133	2.366LeuMet: 2.366 ± 0.058	5.902LeuAsn: 5.902 ± 0.102	2.847LeuPro: 2.847 ± 0.068	2.162LeuGln: 2.162 ± 0.064	2.971LeuArg: 2.971 ± 0.065	6.603LeuSer: 6.603 ± 0.094	4.475LeuThr: 4.475 ± 0.071	5.21LeuVal: 5.21 ± 0.089	0.586LeuTrp: 0.586 ± 0.027	3.284LeuTyr: 3.284 ± 0.078	0.0LeuXaa: 0.0 ± 0.0
Met	1.81MetAla: 1.81 ± 0.055	0.335MetCys: 0.335 ± 0.021	1.575MetAsp: 1.575 ± 0.049	1.822MetGlu: 1.822 ± 0.048	1.086MetPhe: 1.086 ± 0.038	1.767MetGly: 1.767 ± 0.052	0.373MetHis: 0.373 ± 0.023	2.149MetIle: 2.149 ± 0.056	2.772MetLys: 2.772 ± 0.066	2.446MetLeu: 2.446 ± 0.062	0.631MetMet: 0.631 ± 0.031	1.756MetAsn: 1.756 ± 0.049	0.957MetPro: 0.957 ± 0.041	0.782MetGln: 0.782 ± 0.031	0.878MetArg: 0.878 ± 0.033	1.857MetSer: 1.857 ± 0.052	1.235MetThr: 1.235 ± 0.041	1.614MetVal: 1.614 ± 0.049	0.158MetTrp: 0.158 ± 0.016	0.926MetTyr: 0.926 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	3.166AsnAla: 3.166 ± 0.07	0.855AsnCys: 0.855 ± 0.037	3.205AsnAsp: 3.205 ± 0.065	4.201AsnGlu: 4.201 ± 0.085	2.635AsnPhe: 2.635 ± 0.067	3.865AsnGly: 3.865 ± 0.076	0.769AsnHis: 0.769 ± 0.036	6.735AsnIle: 6.735 ± 0.12	6.177AsnLys: 6.177 ± 0.105	5.487AsnLeu: 5.487 ± 0.083	1.702AsnMet: 1.702 ± 0.046	4.273AsnAsn: 4.273 ± 0.092	2.071AsnPro: 2.071 ± 0.062	1.304AsnGln: 1.304 ± 0.04	1.893AsnArg: 1.893 ± 0.052	4.327AsnSer: 4.327 ± 0.099	3.036AsnThr: 3.036 ± 0.069	3.832AsnVal: 3.832 ± 0.073	0.488AsnTrp: 0.488 ± 0.026	2.928AsnTyr: 2.928 ± 0.072	0.0AsnXaa: 0.0 ± 0.0
Pro	1.493ProAla: 1.493 ± 0.056	0.316ProCys: 0.316 ± 0.02	1.59ProAsp: 1.59 ± 0.052	2.208ProGlu: 2.208 ± 0.059	1.454ProPhe: 1.454 ± 0.048	1.751ProGly: 1.751 ± 0.051	0.466ProHis: 0.466 ± 0.024	2.749ProIle: 2.749 ± 0.063	2.434ProLys: 2.434 ± 0.06	2.402ProLeu: 2.402 ± 0.055	0.812ProMet: 0.812 ± 0.034	1.524ProAsn: 1.524 ± 0.048	0.618ProPro: 0.618 ± 0.032	0.897ProGln: 0.897 ± 0.037	0.767ProArg: 0.767 ± 0.033	1.827ProSer: 1.827 ± 0.051	1.493ProThr: 1.493 ± 0.044	2.206ProVal: 2.206 ± 0.058	0.267ProTrp: 0.267 ± 0.021	1.332ProTyr: 1.332 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	1.519GlnAla: 1.519 ± 0.049	0.286GlnCys: 0.286 ± 0.019	1.212GlnAsp: 1.212 ± 0.041	1.446GlnGlu: 1.446 ± 0.054	1.122GlnPhe: 1.122 ± 0.042	1.385GlnGly: 1.385 ± 0.045	0.299GlnHis: 0.299 ± 0.018	2.249GlnIle: 2.249 ± 0.047	2.21GlnLys: 2.21 ± 0.062	2.051GlnLeu: 2.051 ± 0.055	0.653GlnMet: 0.653 ± 0.027	1.704GlnAsn: 1.704 ± 0.046	0.603GlnPro: 0.603 ± 0.032	0.708GlnGln: 0.708 ± 0.038	0.922GlnArg: 0.922 ± 0.035	1.454GlnSer: 1.454 ± 0.043	1.091GlnThr: 1.091 ± 0.038	1.441GlnVal: 1.441 ± 0.043	0.197GlnTrp: 0.197 ± 0.016	1.11GlnTyr: 1.11 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	1.83ArgAla: 1.83 ± 0.055	0.394ArgCys: 0.394 ± 0.024	1.629ArgAsp: 1.629 ± 0.047	2.643ArgGlu: 2.643 ± 0.06	1.397ArgPhe: 1.397 ± 0.041	1.869ArgGly: 1.869 ± 0.052	0.489ArgHis: 0.489 ± 0.028	3.139ArgIle: 3.139 ± 0.066	3.32ArgLys: 3.32 ± 0.056	2.757ArgLeu: 2.757 ± 0.059	0.966ArgMet: 0.966 ± 0.042	2.059ArgAsn: 2.059 ± 0.05	0.899ArgPro: 0.899 ± 0.038	0.94ArgGln: 0.94 ± 0.036	1.432ArgArg: 1.432 ± 0.05	1.575ArgSer: 1.575 ± 0.048	1.629ArgThr: 1.629 ± 0.048	2.157ArgVal: 2.157 ± 0.065	0.272ArgTrp: 0.272 ± 0.022	1.343ArgTyr: 1.343 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.521SerAla: 3.521 ± 0.075	0.755SerCys: 0.755 ± 0.036	3.44SerAsp: 3.44 ± 0.064	4.294SerGlu: 4.294 ± 0.087	3.095SerPhe: 3.095 ± 0.07	4.473SerGly: 4.473 ± 0.084	0.937SerHis: 0.937 ± 0.037	6.688SerIle: 6.688 ± 0.106	6.152SerLys: 6.152 ± 0.097	5.614SerLeu: 5.614 ± 0.098	1.791SerMet: 1.791 ± 0.05	3.975SerAsn: 3.975 ± 0.078	1.69SerPro: 1.69 ± 0.042	1.751SerGln: 1.751 ± 0.055	2.113SerArg: 2.113 ± 0.051	4.904SerSer: 4.904 ± 0.115	3.421SerThr: 3.421 ± 0.075	4.067SerVal: 4.067 ± 0.078	0.472SerTrp: 0.472 ± 0.026	2.542SerTyr: 2.542 ± 0.071	0.0SerXaa: 0.0 ± 0.0
Thr	3.31ThrAla: 3.31 ± 0.075	0.523ThrCys: 0.523 ± 0.029	2.39ThrAsp: 2.39 ± 0.055	2.865ThrGlu: 2.865 ± 0.073	2.217ThrPhe: 2.217 ± 0.059	3.65ThrGly: 3.65 ± 0.08	0.708ThrHis: 0.708 ± 0.034	4.65ThrIle: 4.65 ± 0.083	3.717ThrLys: 3.717 ± 0.058	4.515ThrLeu: 4.515 ± 0.08	1.209ThrMet: 1.209 ± 0.042	2.696ThrAsn: 2.696 ± 0.068	1.775ThrPro: 1.775 ± 0.046	1.142ThrGln: 1.142 ± 0.044	1.462ThrArg: 1.462 ± 0.04	3.449ThrSer: 3.449 ± 0.079	2.688ThrThr: 2.688 ± 0.075	3.48ThrVal: 3.48 ± 0.074	0.374ThrTrp: 0.374 ± 0.022	2.055ThrTyr: 2.055 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	3.783ValAla: 3.783 ± 0.084	0.948ValCys: 0.948 ± 0.036	3.893ValAsp: 3.893 ± 0.079	4.327ValGlu: 4.327 ± 0.077	3.098ValPhe: 3.098 ± 0.069	4.197ValGly: 4.197 ± 0.096	0.908ValHis: 0.908 ± 0.036	6.332ValIle: 6.332 ± 0.099	5.815ValLys: 5.815 ± 0.097	5.841ValLeu: 5.841 ± 0.105	1.676ValMet: 1.676 ± 0.053	3.922ValAsn: 3.922 ± 0.078	2.213ValPro: 2.213 ± 0.055	1.582ValGln: 1.582 ± 0.049	2.032ValArg: 2.032 ± 0.048	4.634ValSer: 4.634 ± 0.083	3.263ValThr: 3.263 ± 0.068	4.744ValVal: 4.744 ± 0.093	0.428ValTrp: 0.428 ± 0.027	2.505ValTyr: 2.505 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.377TrpAla: 0.377 ± 0.025	0.114TrpCys: 0.114 ± 0.013	0.385TrpAsp: 0.385 ± 0.025	0.362TrpGlu: 0.362 ± 0.025	0.331TrpPhe: 0.331 ± 0.021	0.547TrpGly: 0.547 ± 0.025	0.138TrpHis: 0.138 ± 0.014	0.649TrpIle: 0.649 ± 0.028	0.651TrpLys: 0.651 ± 0.033	0.625TrpLeu: 0.625 ± 0.03	0.237TrpMet: 0.237 ± 0.016	0.512TrpAsn: 0.512 ± 0.027	0.182TrpPro: 0.182 ± 0.015	0.253TrpGln: 0.253 ± 0.018	0.263TrpArg: 0.263 ± 0.017	0.416TrpSer: 0.416 ± 0.025	0.35TrpThr: 0.35 ± 0.024	0.429TrpVal: 0.429 ± 0.027	0.084TrpTrp: 0.084 ± 0.01	0.261TrpTyr: 0.261 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.129TyrAla: 2.129 ± 0.046	0.542TyrCys: 0.542 ± 0.028	2.354TyrAsp: 2.354 ± 0.057	2.82TyrGlu: 2.82 ± 0.076	2.086TyrPhe: 2.086 ± 0.062	2.627TyrGly: 2.627 ± 0.067	0.587TyrHis: 0.587 ± 0.03	3.899TyrIle: 3.899 ± 0.079	3.867TyrLys: 3.867 ± 0.078	3.535TyrLeu: 3.535 ± 0.084	1.115TyrMet: 1.115 ± 0.04	2.693TyrAsn: 2.693 ± 0.076	1.284TyrPro: 1.284 ± 0.045	0.767TyrGln: 0.767 ± 0.039	1.424TyrArg: 1.424 ± 0.046	2.894TyrSer: 2.894 ± 0.065	2.016TyrThr: 2.016 ± 0.051	2.504TyrVal: 2.504 ± 0.063	0.292TyrTrp: 0.292 ± 0.02	1.857TyrTyr: 1.857 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2713 proteins (745997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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