Albimonas donghaensis
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4253 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3FFE6|A0A1H3FFE6_9RHOB Uncharacterized protein OS=Albimonas donghaensis OX=356660 GN=SAMN05444336_11287 PE=4 SV=1
MM1 pKa = 6.95 GTFAVTYY8 pKa = 9.65 GYY10 pKa = 10.79 FSFGGWSSSKK20 pKa = 10.7 GGYY23 pKa = 9.22 SSSGKK28 pKa = 9.99 CDD30 pKa = 2.73 WGYY33 pKa = 11.52 SSSSWGGSSKK43 pKa = 10.75 GSSHH47 pKa = 6.64 GSKK50 pKa = 10.5 GWGGHH55 pKa = 5.38 NKK57 pKa = 10.2 SNDD60 pKa = 3.51 CDD62 pKa = 2.98 WGYY65 pKa = 11.24 GSKK68 pKa = 10.88 GGGGGSKK75 pKa = 9.93 KK76 pKa = 9.71 HH77 pKa = 6.92 DD78 pKa = 3.74 SKK80 pKa = 11.45 SWCDD84 pKa = 3.82 DD85 pKa = 2.52 GWGGSKK91 pKa = 10.64 GGWGGSSWDD100 pKa = 3.45 DD101 pKa = 3.76 CGWGGGHH108 pKa = 6.55 SGGGHH113 pKa = 6.22 GGGHH117 pKa = 6.7 GGGGHH122 pKa = 6.81 GGGGHH127 pKa = 6.15 GGGWGGGGWGGGWGCDD143 pKa = 3.42 VNAEE147 pKa = 4.26 PVFEE151 pKa = 5.39 DD152 pKa = 4.2 DD153 pKa = 4.73 CNACIEE159 pKa = 4.19 VTIDD163 pKa = 3.24 EE164 pKa = 4.4 NTLAVIDD171 pKa = 5.1 LDD173 pKa = 3.87 ATDD176 pKa = 4.92 ADD178 pKa = 4.68 GDD180 pKa = 4.05 TLTYY184 pKa = 9.9 EE185 pKa = 4.07 ISGGDD190 pKa = 3.57 DD191 pKa = 2.99 AALFTVDD198 pKa = 3.94 PATGEE203 pKa = 4.09 LTFIDD208 pKa = 4.63 APDD211 pKa = 4.06 FEE213 pKa = 6.46 APLDD217 pKa = 4.03 ADD219 pKa = 3.62 GDD221 pKa = 3.84 NVYY224 pKa = 10.64 DD225 pKa = 3.87 VKK227 pKa = 11.6 VKK229 pKa = 9.67 VTDD232 pKa = 3.9 GEE234 pKa = 4.51 GGSDD238 pKa = 3.56 EE239 pKa = 5.22 ACVKK243 pKa = 10.79 VEE245 pKa = 4.37 VEE247 pKa = 4.95 DD248 pKa = 5.04 VDD250 pKa = 4.24 DD251 pKa = 4.48 TPPPSGGGACQFTLSQDD268 pKa = 3.39 GLDD271 pKa = 3.81 IEE273 pKa = 4.64 VHH275 pKa = 5.07 LTEE278 pKa = 5.48 LPDD281 pKa = 3.85 GSVQIEE287 pKa = 4.25 LAVADD292 pKa = 4.96 DD293 pKa = 3.89 SAKK296 pKa = 11.02 VGDD299 pKa = 3.68 LRR301 pKa = 11.84 GLFFHH306 pKa = 6.78 VADD309 pKa = 4.11 EE310 pKa = 4.45 SKK312 pKa = 10.56 IGGLRR317 pKa = 11.84 ITGDD321 pKa = 3.79 DD322 pKa = 3.52 VTGTQYY328 pKa = 11.29 CEE330 pKa = 4.97 DD331 pKa = 3.8 RR332 pKa = 11.84 VKK334 pKa = 11.26 DD335 pKa = 4.09 LGWGANVSGLGRR347 pKa = 11.84 FDD349 pKa = 4.81 AGVKK353 pKa = 10.3 LGTSGIGKK361 pKa = 9.83 DD362 pKa = 4.23 DD363 pKa = 3.4 IRR365 pKa = 11.84 STTLVISHH373 pKa = 7.88 PDD375 pKa = 3.28 GMSLDD380 pKa = 4.03 DD381 pKa = 5.77 FRR383 pKa = 11.84 NQDD386 pKa = 3.08 FAIRR390 pKa = 11.84 ATSVGYY396 pKa = 10.13 EE397 pKa = 4.15 GGWRR401 pKa = 11.84 GDD403 pKa = 3.53 SEE405 pKa = 5.24 KK406 pKa = 10.7 IGGNAGEE413 pKa = 4.66 LDD415 pKa = 3.85 CGGANANSAPIAVDD429 pKa = 4.35 DD430 pKa = 4.51 EE431 pKa = 5.14 GGLCSTDD438 pKa = 2.77 ATLIDD443 pKa = 3.94 VLANDD448 pKa = 4.19 SDD450 pKa = 4.27 ADD452 pKa = 3.91 PDD454 pKa = 4.25 EE455 pKa = 4.26 VWGLLGIKK463 pKa = 8.84 TDD465 pKa = 3.49 AGIEE469 pKa = 4.11 FMTDD473 pKa = 2.66 GDD475 pKa = 4.97 SFTLDD480 pKa = 2.66 SGAVVTRR487 pKa = 11.84 VGDD490 pKa = 3.45 AFEE493 pKa = 4.81 YY494 pKa = 10.73 DD495 pKa = 3.43 LSGTGDD501 pKa = 3.92 DD502 pKa = 5.45 FDD504 pKa = 4.52 TLLVGQKK511 pKa = 10.39 GADD514 pKa = 3.21 SFEE517 pKa = 4.2 YY518 pKa = 10.91 VIGDD522 pKa = 3.89 DD523 pKa = 5.01 EE524 pKa = 4.71 GATDD528 pKa = 3.3 IGEE531 pKa = 4.22 VVIDD535 pKa = 3.25 ICGGVNAVEE544 pKa = 6.1 LIDD547 pKa = 4.6 NDD549 pKa = 3.98 NPLLASMDD557 pKa = 3.93 LGQVLDD563 pKa = 3.91 PVSWLVEE570 pKa = 4.22 TVNAAVDD577 pKa = 4.13 FDD579 pKa = 3.93 EE580 pKa = 4.79 TAAEE584 pKa = 4.05 ALLDD588 pKa = 4.24 LGALGLTDD596 pKa = 4.25 HH597 pKa = 7.2 GIDD600 pKa = 3.64 GLFGPAYY607 pKa = 10.06 CVDD610 pKa = 3.37 RR611 pKa = 11.84 TRR613 pKa = 11.84 PANYY617 pKa = 9.15 DD618 pKa = 3.14 ATDD621 pKa = 3.45 ALVYY625 pKa = 10.08 TSTGDD630 pKa = 3.32 IPDD633 pKa = 3.56 VNFDD637 pKa = 3.7 GGDD640 pKa = 3.67 VIDD643 pKa = 5.39 KK644 pKa = 10.83 EE645 pKa = 4.46 EE646 pKa = 4.65 NLDD649 pKa = 3.7 NLNWLLSQRR658 pKa = 11.84 YY659 pKa = 7.91 EE660 pKa = 4.13 DD661 pKa = 3.31 QGYY664 pKa = 9.7 GYY666 pKa = 9.07 TDD668 pKa = 3.11 IQSAIWQLMDD678 pKa = 4.11 DD679 pKa = 3.99 VGGIDD684 pKa = 3.73 TSRR687 pKa = 11.84 STPGVQLTAAAQTMYY702 pKa = 10.65 DD703 pKa = 3.1 AAMANGDD710 pKa = 3.63 GFAPTGADD718 pKa = 3.19 GDD720 pKa = 4.16 VLGLIFQPVSDD731 pKa = 4.54 NGSGGYY737 pKa = 9.24 DD738 pKa = 3.02 ANGQIFVVGYY748 pKa = 10.19 DD749 pKa = 3.91 LQDD752 pKa = 3.79 CLCYY756 pKa = 10.58
Molecular weight: 77.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.63
IPC_protein 3.694
Toseland 3.452
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.35
Solomon 3.706
Lehninger 3.656
Nozaki 3.808
DTASelect 4.113
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.821
Patrickios 1.558
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.75
Protein with the highest isoelectric point:
>tr|A0A1H3A3J3|A0A1H3A3J3_9RHOB Histidine kinase OS=Albimonas donghaensis OX=356660 GN=SAMN05444336_10423 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.48 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.42 NGAKK29 pKa = 9.88 IIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.25 GRR39 pKa = 11.84 HH40 pKa = 5.49 KK41 pKa = 10.9 LSAA44 pKa = 3.8
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4253
0
4253
1441178
31
9789
338.9
36.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.395 ± 0.082
0.817 ± 0.013
5.96 ± 0.052
5.999 ± 0.038
3.442 ± 0.019
9.762 ± 0.078
1.799 ± 0.018
4.259 ± 0.028
2.251 ± 0.036
9.971 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.576 ± 0.022
1.931 ± 0.023
5.964 ± 0.049
2.349 ± 0.022
7.777 ± 0.058
4.722 ± 0.035
4.819 ± 0.033
6.951 ± 0.035
1.424 ± 0.018
1.83 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here