Human fecal virus Jorvi4
Average proteome isoelectric point is 7.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGD7|A0A220IGD7_9CIRC Uncharacterized protein OS=Human fecal virus Jorvi4 OX=2017083 PE=4 SV=1
MM1 pKa = 7.44 SRR3 pKa = 11.84 YY4 pKa = 9.7 QSICFTIFDD13 pKa = 3.92 PHH15 pKa = 7.66 IVNDD19 pKa = 4.56 DD20 pKa = 3.7 QVCMQRR26 pKa = 11.84 AYY28 pKa = 10.02 DD29 pKa = 3.63 TGKK32 pKa = 10.82 LGFLVYY38 pKa = 10.47 QIEE41 pKa = 4.36 RR42 pKa = 11.84 CPNTNRR48 pKa = 11.84 EE49 pKa = 4.33 HH50 pKa = 5.58 IQGYY54 pKa = 10.41 AEE56 pKa = 3.99 LHH58 pKa = 5.95 SRR60 pKa = 11.84 TTLGRR65 pKa = 11.84 HH66 pKa = 5.65 GPRR69 pKa = 11.84 SSGVKK74 pKa = 10.33 GIFRR78 pKa = 11.84 SDD80 pKa = 3.12 TMHH83 pKa = 7.9 VEE85 pKa = 4.05 MAKK88 pKa = 10.68 GSAEE92 pKa = 4.01 QNITYY97 pKa = 7.63 CTKK100 pKa = 8.95 PEE102 pKa = 3.86 SRR104 pKa = 11.84 VRR106 pKa = 11.84 EE107 pKa = 4.11 GLRR110 pKa = 11.84 LGEE113 pKa = 4.3 PANKK117 pKa = 10.09 RR118 pKa = 11.84 PGEE121 pKa = 4.09 RR122 pKa = 11.84 KK123 pKa = 9.7 DD124 pKa = 3.25 WEE126 pKa = 4.31 EE127 pKa = 4.15 LYY129 pKa = 10.1 EE130 pKa = 4.16 HH131 pKa = 7.42 CIHH134 pKa = 7.81 ADD136 pKa = 3.26 TDD138 pKa = 3.9 EE139 pKa = 4.71 KK140 pKa = 11.04 NLCIGHH146 pKa = 6.79 PVLIKK151 pKa = 9.73 YY152 pKa = 8.46 YY153 pKa = 10.53 RR154 pKa = 11.84 SATEE158 pKa = 3.29 IWSRR162 pKa = 11.84 THH164 pKa = 6.28 PPSMRR169 pKa = 11.84 FPIRR173 pKa = 11.84 IFILVGPTRR182 pKa = 11.84 TGKK185 pKa = 8.0 TRR187 pKa = 11.84 WAFTKK192 pKa = 10.47 FPEE195 pKa = 4.84 LYY197 pKa = 10.13 RR198 pKa = 11.84 LCTEE202 pKa = 5.1 KK203 pKa = 11.05 ADD205 pKa = 3.16 WWNGYY210 pKa = 9.35 LGQKK214 pKa = 10.03 DD215 pKa = 3.81 VLIDD219 pKa = 3.64 EE220 pKa = 5.17 FDD222 pKa = 3.95 GEE224 pKa = 4.87 SIKK227 pKa = 9.96 LTYY230 pKa = 9.57 MLQLLDD236 pKa = 4.68 RR237 pKa = 11.84 YY238 pKa = 9.84 PMQVPTKK245 pKa = 9.37 GGFVRR250 pKa = 11.84 LCATNWIITSNIDD263 pKa = 2.92 HH264 pKa = 6.74 DD265 pKa = 3.92 QWYY268 pKa = 11.27 SMAPQGNRR276 pKa = 11.84 DD277 pKa = 3.17 AFYY280 pKa = 11.36 ARR282 pKa = 11.84 IQEE285 pKa = 4.09 WGRR288 pKa = 11.84 VLSVVEE294 pKa = 4.0 YY295 pKa = 10.97 RR296 pKa = 11.84 EE297 pKa = 4.15 DD298 pKa = 3.47 LQLEE302 pKa = 4.31 EE303 pKa = 4.56 QEE305 pKa = 4.46 GAQAQEE311 pKa = 3.89
Molecular weight: 36.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.134
IPC2_protein 6.148
IPC_protein 6.249
Toseland 6.478
ProMoST 6.478
Dawson 6.59
Bjellqvist 6.561
Wikipedia 6.605
Rodwell 6.59
Grimsley 6.59
Solomon 6.605
Lehninger 6.605
Nozaki 6.868
DTASelect 7.029
Thurlkill 7.059
EMBOSS 7.029
Sillero 6.985
Patrickios 4.062
IPC_peptide 6.62
IPC2_peptide 6.868
IPC2.peptide.svr19 6.81
Protein with the highest isoelectric point:
>tr|A0A220IGD7|A0A220IGD7_9CIRC Uncharacterized protein OS=Human fecal virus Jorvi4 OX=2017083 PE=4 SV=1
MM1 pKa = 7.51 ARR3 pKa = 11.84 RR4 pKa = 11.84 GYY6 pKa = 10.1 RR7 pKa = 11.84 YY8 pKa = 9.73 KK9 pKa = 10.53 RR10 pKa = 11.84 YY11 pKa = 9.38 YY12 pKa = 9.85 GKK14 pKa = 9.86 NRR16 pKa = 11.84 KK17 pKa = 9.07 RR18 pKa = 11.84 FIGPLLPGGYY28 pKa = 10.32 AKK30 pKa = 10.93 GNAKK34 pKa = 10.07 SLARR38 pKa = 11.84 KK39 pKa = 6.58 WRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 YY45 pKa = 9.89 RR46 pKa = 11.84 YY47 pKa = 9.48 GKK49 pKa = 10.01 KK50 pKa = 9.92 SKK52 pKa = 10.37 QSLRR56 pKa = 11.84 SGWGLGMTRR65 pKa = 11.84 AKK67 pKa = 10.75 SFVKK71 pKa = 10.32 RR72 pKa = 11.84 VDD74 pKa = 3.86 LNEE77 pKa = 3.93 FTPKK81 pKa = 9.98 GSCRR85 pKa = 11.84 FRR87 pKa = 11.84 ISYY90 pKa = 10.1 RR91 pKa = 11.84 GRR93 pKa = 11.84 HH94 pKa = 6.51 LITAANVYY102 pKa = 9.68 CAANFHH108 pKa = 6.22 PHH110 pKa = 5.4 PHH112 pKa = 5.94 YY113 pKa = 8.05 PTTGFADD120 pKa = 3.86 PVLSATSDD128 pKa = 3.58 DD129 pKa = 3.98 FTSDD133 pKa = 3.35 PRR135 pKa = 11.84 TMDD138 pKa = 2.98 HH139 pKa = 6.79 AGLMGALSNEE149 pKa = 3.24 KK150 pKa = 10.07 FMYY153 pKa = 9.62 FKK155 pKa = 10.11 PVWMKK160 pKa = 9.8 TIFKK164 pKa = 10.53 VRR166 pKa = 11.84 VTGGADD172 pKa = 3.42 PVWTCEE178 pKa = 3.83 TMGRR182 pKa = 11.84 KK183 pKa = 8.87 CEE185 pKa = 4.0 YY186 pKa = 10.06 SSSGLPDD193 pKa = 4.97 PYY195 pKa = 10.42 TEE197 pKa = 4.83 SKK199 pKa = 10.65 IEE201 pKa = 3.97 TDD203 pKa = 4.34 FKK205 pKa = 11.1 TKK207 pKa = 9.7 MSFYY211 pKa = 10.77 KK212 pKa = 10.34 ILTAGKK218 pKa = 7.16 TCKK221 pKa = 9.16 YY222 pKa = 9.29 TLFSRR227 pKa = 11.84 LGEE230 pKa = 4.18 NKK232 pKa = 10.38 SIGPGSLQNPAATLISNYY250 pKa = 9.41 IKK252 pKa = 10.44 PSQWLLWNEE261 pKa = 4.85 TIQNMGNWLNYY272 pKa = 8.97 PVHH275 pKa = 6.25 QLIVKK280 pKa = 9.83 SGSFGIGAVDD290 pKa = 3.82 AQRR293 pKa = 11.84 MGLYY297 pKa = 9.87 VEE299 pKa = 5.94 AYY301 pKa = 7.75 HH302 pKa = 6.58 TVHH305 pKa = 7.02 CLVAKK310 pKa = 10.68 NNAVTQAYY318 pKa = 7.35 NTAA321 pKa = 3.61
Molecular weight: 36.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.26
IPC2_protein 9.663
IPC_protein 9.838
Toseland 10.16
ProMoST 9.882
Dawson 10.365
Bjellqvist 10.058
Wikipedia 10.555
Rodwell 10.789
Grimsley 10.452
Solomon 10.394
Lehninger 10.35
Nozaki 10.175
DTASelect 10.058
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.277
Patrickios 10.321
IPC_peptide 10.394
IPC2_peptide 8.887
IPC2.peptide.svr19 8.597
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
632
311
321
316.0
36.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.171 ± 0.923
2.215 ± 0.245
4.272 ± 0.818
5.38 ± 1.821
3.956 ± 0.287
7.911 ± 0.574
2.848 ± 0.252
5.063 ± 0.937
6.487 ± 1.36
6.962 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.848 ± 0.189
4.114 ± 0.615
4.905 ± 0.056
3.639 ± 1.031
7.911 ± 0.307
6.013 ± 0.815
6.804 ± 0.255
4.589 ± 0.06
2.373 ± 0.136
5.538 ± 0.49
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here