Mycobacterium Phage Rachaly

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EIB8|A0A4Y6EIB8_9CAUD Uncharacterized protein OS=Mycobacterium Phage Rachaly OX=2588140 GN=91 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.4AIILACAFAAGGLIGQHH19 pKa = 5.95IPVTVNSTEE28 pKa = 3.54VRR30 pKa = 11.84TVDD33 pKa = 4.14GIDD36 pKa = 3.6YY37 pKa = 7.63PVCAVEE43 pKa = 5.17DD44 pKa = 4.64CSDD47 pKa = 3.31QPGQIGIWIDD57 pKa = 3.69PDD59 pKa = 3.84TGNQWLSHH67 pKa = 6.31GEE69 pKa = 3.87TSQLIEE75 pKa = 3.99RR76 pKa = 4.68

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EM01|A0A4Y6EM01_9CAUD Helix-turn-helix DNA binding domain protein OS=Mycobacterium Phage Rachaly OX=2588140 GN=46 PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 3.5VSFHH6 pKa = 5.76GSARR10 pKa = 11.84LRR12 pKa = 11.84QAEE15 pKa = 4.24AATHH19 pKa = 5.84NRR21 pKa = 11.84DD22 pKa = 4.11KK23 pKa = 11.02FRR25 pKa = 11.84TWLEE29 pKa = 3.59RR30 pKa = 11.84AGASTAPRR38 pKa = 11.84WLSRR42 pKa = 11.84EE43 pKa = 3.73SWLKK47 pKa = 11.39DD48 pKa = 2.78MTEE51 pKa = 3.85WSRR54 pKa = 11.84SDD56 pKa = 3.11RR57 pKa = 11.84FTAAKK62 pKa = 9.22EE63 pKa = 3.91SAGISMTAPTFMAIMRR79 pKa = 11.84VMADD83 pKa = 3.58YY84 pKa = 11.2ADD86 pKa = 4.29HH87 pKa = 7.41SDD89 pKa = 3.64GRR91 pKa = 11.84CVAVSRR97 pKa = 11.84EE98 pKa = 4.14RR99 pKa = 11.84IASEE103 pKa = 4.17AGCSPRR109 pKa = 11.84TVTNAWRR116 pKa = 11.84LLRR119 pKa = 11.84LSGFGIEE126 pKa = 4.23AQRR129 pKa = 11.84GHH131 pKa = 6.51GSDD134 pKa = 3.2RR135 pKa = 11.84TPTQGCRR142 pKa = 11.84PSIYY146 pKa = 10.24HH147 pKa = 6.41LVSKK151 pKa = 10.18RR152 pKa = 11.84KK153 pKa = 8.78PVVGFCYY160 pKa = 10.38LPPKK164 pKa = 10.38AGVSSSSPVGNNSPRR179 pKa = 11.84AATRR183 pKa = 11.84PRR185 pKa = 11.84PIEE188 pKa = 3.82IQRR191 pKa = 11.84LAARR195 pKa = 11.84LAPMVPGYY203 pKa = 9.42RR204 pKa = 11.84HH205 pKa = 5.26WHH207 pKa = 5.91LCDD210 pKa = 3.5ALTAAGIDD218 pKa = 3.64PGEE221 pKa = 4.05WTAGRR226 pKa = 11.84LYY228 pKa = 10.46EE229 pKa = 4.66ALNARR234 pKa = 11.84PWRR237 pKa = 11.84PDD239 pKa = 3.0TPGSVGYY246 pKa = 10.31LRR248 pKa = 11.84AALRR252 pKa = 11.84RR253 pKa = 11.84VAGEE257 pKa = 4.08KK258 pKa = 7.38TTAAAPPARR267 pKa = 11.84TEE269 pKa = 3.9EE270 pKa = 4.14RR271 pKa = 11.84TVPASAEE278 pKa = 3.82TRR280 pKa = 11.84AWAMAQMFGRR290 pKa = 11.84NRR292 pKa = 11.84HH293 pKa = 4.57SDD295 pKa = 2.86IRR297 pKa = 11.84GLL299 pKa = 3.65

Molecular weight: 32.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

16126

34

842

177.2

19.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.947 ± 0.406

0.943 ± 0.116

6.263 ± 0.205

6.666 ± 0.351

3.367 ± 0.185

8.328 ± 0.473

1.997 ± 0.148

5.11 ± 0.199

4.626 ± 0.254

8.148 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.128

3.293 ± 0.18

5.569 ± 0.297

3.857 ± 0.254

6.226 ± 0.414

5.438 ± 0.182

6.114 ± 0.205

7.144 ± 0.286

1.86 ± 0.141

2.815 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski