Burkholderia phage AMP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Okabevirinae; Ampunavirus; unclassified Ampunavirus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C2IBQ3|A0A5C2IBQ3_9CAUD Putative transcription regulator OS=Burkholderia phage AMP1 OX=2601683 GN=AMP1_14 PE=4 SV=1
MM1 pKa = 6.93NTILNIGLARR11 pKa = 11.84NDD13 pKa = 3.71GKK15 pKa = 10.12PDD17 pKa = 3.33NGVLHH22 pKa = 6.34VVASLNEE29 pKa = 3.96YY30 pKa = 10.11GFKK33 pKa = 10.6LVAGEE38 pKa = 4.2VFEE41 pKa = 4.41VTHH44 pKa = 5.65AHH46 pKa = 6.1GVEE49 pKa = 3.81QTLIAEE55 pKa = 4.36VEE57 pKa = 4.3YY58 pKa = 10.48QHH60 pKa = 6.71NAIYY64 pKa = 10.57LPAAVKK70 pKa = 10.52ALADD74 pKa = 4.4EE75 pKa = 4.88LAQDD79 pKa = 5.01CIAVGYY85 pKa = 9.25LQDD88 pKa = 3.61GTLQGGDD95 pKa = 3.48LYY97 pKa = 11.26GSKK100 pKa = 10.21AAEE103 pKa = 3.69WGPFNPQYY111 pKa = 10.31FFCPTRR117 pKa = 3.85

Molecular weight: 12.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C2IBL8|A0A5C2IBL8_9CAUD Portal protein OS=Burkholderia phage AMP1 OX=2601683 GN=AMP1_31 PE=4 SV=1
MM1 pKa = 7.38LGKK4 pKa = 10.19VEE6 pKa = 4.0NWSNRR11 pKa = 11.84IGRR14 pKa = 11.84GVEE17 pKa = 3.41PRR19 pKa = 11.84TFLKK23 pKa = 10.85NVLTYY28 pKa = 10.58LVYY31 pKa = 10.4HH32 pKa = 7.02AEE34 pKa = 4.1NPSNLKK40 pKa = 10.62YY41 pKa = 8.37PTHH44 pKa = 5.67KK45 pKa = 9.78TEE47 pKa = 4.12AEE49 pKa = 3.86KK50 pKa = 10.76RR51 pKa = 11.84DD52 pKa = 3.32ARR54 pKa = 11.84NRR56 pKa = 11.84KK57 pKa = 8.85ARR59 pKa = 11.84LARR62 pKa = 11.84RR63 pKa = 11.84KK64 pKa = 10.09AKK66 pKa = 10.32EE67 pKa = 3.73AVV69 pKa = 3.17

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12740

55

1665

265.4

29.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.389 ± 0.584

0.887 ± 0.167

6.06 ± 0.268

5.848 ± 0.368

3.352 ± 0.176

8.053 ± 0.373

1.884 ± 0.142

4.474 ± 0.206

5.275 ± 0.435

8.14 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.15

4.49 ± 0.246

4.388 ± 0.255

4.403 ± 0.355

6.374 ± 0.39

5.659 ± 0.245

5.581 ± 0.273

6.86 ± 0.309

1.523 ± 0.108

3.148 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski