Amino acid dipepetide frequency for Burkholderia phage AMP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.819AlaAla: 17.819 ± 2.188
0.863AlaCys: 0.863 ± 0.273
6.437AlaAsp: 6.437 ± 1.069
6.044AlaGlu: 6.044 ± 0.855
2.904AlaPhe: 2.904 ± 0.374
8.478AlaGly: 8.478 ± 1.032
1.57AlaHis: 1.57 ± 0.368
4.631AlaIle: 4.631 ± 0.546
4.788AlaLys: 4.788 ± 0.906
10.597AlaLeu: 10.597 ± 1.373
2.747AlaMet: 2.747 ± 0.562
6.044AlaAsn: 6.044 ± 1.164
4.239AlaPro: 4.239 ± 0.944
4.317AlaGln: 4.317 ± 0.848
7.379AlaArg: 7.379 ± 0.776
6.515AlaSer: 6.515 ± 0.684
5.416AlaThr: 5.416 ± 0.848
7.3AlaVal: 7.3 ± 0.793
1.962AlaTrp: 1.962 ± 0.3
3.846AlaTyr: 3.846 ± 0.55
0.0AlaXaa: 0.0 ± 0.0
Cys
1.099CysAla: 1.099 ± 0.433
0.078CysCys: 0.078 ± 0.101
0.314CysAsp: 0.314 ± 0.162
0.471CysGlu: 0.471 ± 0.238
0.235CysPhe: 0.235 ± 0.139
0.785CysGly: 0.785 ± 0.326
0.471CysHis: 0.471 ± 0.287
0.235CysIle: 0.235 ± 0.119
0.392CysLys: 0.392 ± 0.193
1.02CysLeu: 1.02 ± 0.314
0.314CysMet: 0.314 ± 0.197
0.549CysAsn: 0.549 ± 0.229
0.549CysPro: 0.549 ± 0.238
0.314CysGln: 0.314 ± 0.155
0.392CysArg: 0.392 ± 0.199
0.314CysSer: 0.314 ± 0.177
0.157CysThr: 0.157 ± 0.11
0.706CysVal: 0.706 ± 0.286
0.235CysTrp: 0.235 ± 0.136
0.235CysTyr: 0.235 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
7.536AspAla: 7.536 ± 1.113
0.392AspCys: 0.392 ± 0.192
4.631AspAsp: 4.631 ± 0.967
3.454AspGlu: 3.454 ± 0.531
2.59AspPhe: 2.59 ± 0.358
4.945AspGly: 4.945 ± 0.674
0.942AspHis: 0.942 ± 0.249
2.904AspIle: 2.904 ± 0.529
3.218AspLys: 3.218 ± 0.449
4.003AspLeu: 4.003 ± 0.462
1.727AspMet: 1.727 ± 0.363
1.413AspAsn: 1.413 ± 0.265
4.788AspPro: 4.788 ± 0.6
2.59AspGln: 2.59 ± 0.433
2.904AspArg: 2.904 ± 0.499
2.904AspSer: 2.904 ± 0.453
3.218AspThr: 3.218 ± 0.383
3.375AspVal: 3.375 ± 0.503
0.863AspTrp: 0.863 ± 0.279
2.198AspTyr: 2.198 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
5.573GluAla: 5.573 ± 0.807
0.863GluCys: 0.863 ± 0.348
3.768GluAsp: 3.768 ± 0.609
3.768GluGlu: 3.768 ± 0.879
2.276GluPhe: 2.276 ± 0.394
3.846GluGly: 3.846 ± 0.542
1.413GluHis: 1.413 ± 0.313
2.747GluIle: 2.747 ± 0.403
3.454GluLys: 3.454 ± 0.599
5.416GluLeu: 5.416 ± 0.713
0.628GluMet: 0.628 ± 0.195
2.512GluAsn: 2.512 ± 0.552
1.884GluPro: 1.884 ± 0.506
3.689GluGln: 3.689 ± 0.504
4.16GluArg: 4.16 ± 0.78
2.433GluSer: 2.433 ± 0.48
2.904GluThr: 2.904 ± 0.555
4.082GluVal: 4.082 ± 0.67
1.334GluTrp: 1.334 ± 0.408
1.648GluTyr: 1.648 ± 0.402
0.0GluXaa: 0.0 ± 0.0
Phe
3.14PheAla: 3.14 ± 0.458
0.392PheCys: 0.392 ± 0.185
2.355PheAsp: 2.355 ± 0.4
2.59PheGlu: 2.59 ± 0.354
1.099PhePhe: 1.099 ± 0.269
2.904PheGly: 2.904 ± 0.428
0.706PheHis: 0.706 ± 0.174
1.648PheIle: 1.648 ± 0.314
1.57PheLys: 1.57 ± 0.358
1.805PheLeu: 1.805 ± 0.347
0.863PheMet: 0.863 ± 0.232
1.256PheAsn: 1.256 ± 0.382
1.02PhePro: 1.02 ± 0.278
1.177PheGln: 1.177 ± 0.217
2.041PheArg: 2.041 ± 0.407
2.355PheSer: 2.355 ± 0.551
2.198PheThr: 2.198 ± 0.416
2.983PheVal: 2.983 ± 0.434
0.392PheTrp: 0.392 ± 0.176
1.02PheTyr: 1.02 ± 0.254
0.0PheXaa: 0.0 ± 0.0
Gly
8.321GlyAla: 8.321 ± 1.161
0.392GlyCys: 0.392 ± 0.191
4.71GlyAsp: 4.71 ± 0.628
4.396GlyGlu: 4.396 ± 0.75
3.532GlyPhe: 3.532 ± 0.665
8.556GlyGly: 8.556 ± 0.918
1.491GlyHis: 1.491 ± 0.349
3.14GlyIle: 3.14 ± 0.405
4.553GlyLys: 4.553 ± 0.755
4.474GlyLeu: 4.474 ± 0.687
2.198GlyMet: 2.198 ± 0.399
3.375GlyAsn: 3.375 ± 0.531
2.512GlyPro: 2.512 ± 0.667
3.611GlyGln: 3.611 ± 0.417
4.631GlyArg: 4.631 ± 0.774
5.73GlySer: 5.73 ± 0.821
5.495GlyThr: 5.495 ± 0.708
5.809GlyVal: 5.809 ± 0.594
0.942GlyTrp: 0.942 ± 0.278
2.669GlyTyr: 2.669 ± 0.521
0.0GlyXaa: 0.0 ± 0.0
His
1.413HisAla: 1.413 ± 0.319
0.078HisCys: 0.078 ± 0.079
1.334HisAsp: 1.334 ± 0.3
1.413HisGlu: 1.413 ± 0.306
0.706HisPhe: 0.706 ± 0.214
1.805HisGly: 1.805 ± 0.319
0.314HisHis: 0.314 ± 0.154
1.413HisIle: 1.413 ± 0.27
0.785HisLys: 0.785 ± 0.289
1.57HisLeu: 1.57 ± 0.354
0.471HisMet: 0.471 ± 0.207
0.785HisAsn: 0.785 ± 0.25
1.256HisPro: 1.256 ± 0.281
0.392HisGln: 0.392 ± 0.176
0.942HisArg: 0.942 ± 0.28
0.942HisSer: 0.942 ± 0.236
0.942HisThr: 0.942 ± 0.286
1.57HisVal: 1.57 ± 0.37
0.157HisTrp: 0.157 ± 0.114
0.549HisTyr: 0.549 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
5.573IleAla: 5.573 ± 0.606
0.549IleCys: 0.549 ± 0.192
3.689IleAsp: 3.689 ± 0.721
3.846IleGlu: 3.846 ± 0.675
1.099IlePhe: 1.099 ± 0.286
4.239IleGly: 4.239 ± 0.493
0.706IleHis: 0.706 ± 0.179
1.727IleIle: 1.727 ± 0.444
2.512IleLys: 2.512 ± 0.394
2.119IleLeu: 2.119 ± 0.529
0.706IleMet: 0.706 ± 0.237
2.041IleAsn: 2.041 ± 0.333
2.041IlePro: 2.041 ± 0.38
1.177IleGln: 1.177 ± 0.375
2.904IleArg: 2.904 ± 0.597
2.355IleSer: 2.355 ± 0.407
2.669IleThr: 2.669 ± 0.567
3.375IleVal: 3.375 ± 0.383
0.314IleTrp: 0.314 ± 0.203
1.099IleTyr: 1.099 ± 0.375
0.0IleXaa: 0.0 ± 0.0
Lys
7.771LysAla: 7.771 ± 0.918
0.549LysCys: 0.549 ± 0.212
2.119LysAsp: 2.119 ± 0.397
3.218LysGlu: 3.218 ± 0.528
1.491LysPhe: 1.491 ± 0.29
3.768LysGly: 3.768 ± 0.667
0.942LysHis: 0.942 ± 0.317
1.256LysIle: 1.256 ± 0.426
3.375LysLys: 3.375 ± 0.837
5.809LysLeu: 5.809 ± 0.804
0.628LysMet: 0.628 ± 0.211
2.59LysAsn: 2.59 ± 0.502
1.962LysPro: 1.962 ± 0.524
2.276LysGln: 2.276 ± 0.438
3.846LysArg: 3.846 ± 0.714
2.041LysSer: 2.041 ± 0.436
2.198LysThr: 2.198 ± 0.473
3.846LysVal: 3.846 ± 0.428
0.785LysTrp: 0.785 ± 0.213
2.276LysTyr: 2.276 ± 0.452
0.0LysXaa: 0.0 ± 0.0
Leu
8.713LeuAla: 8.713 ± 1.022
0.942LeuCys: 0.942 ± 0.501
5.73LeuAsp: 5.73 ± 0.596
4.239LeuGlu: 4.239 ± 0.782
1.648LeuPhe: 1.648 ± 0.3
5.573LeuGly: 5.573 ± 0.826
1.962LeuHis: 1.962 ± 0.348
3.297LeuIle: 3.297 ± 0.525
4.945LeuLys: 4.945 ± 0.805
6.594LeuLeu: 6.594 ± 0.819
1.648LeuMet: 1.648 ± 0.327
4.003LeuAsn: 4.003 ± 0.656
3.925LeuPro: 3.925 ± 0.735
4.003LeuGln: 4.003 ± 0.529
5.966LeuArg: 5.966 ± 0.65
5.259LeuSer: 5.259 ± 0.585
4.788LeuThr: 4.788 ± 0.49
5.259LeuVal: 5.259 ± 0.77
0.785LeuTrp: 0.785 ± 0.326
2.119LeuTyr: 2.119 ± 0.457
0.0LeuXaa: 0.0 ± 0.0
Met
2.512MetAla: 2.512 ± 0.445
0.078MetCys: 0.078 ± 0.071
1.256MetAsp: 1.256 ± 0.297
1.334MetGlu: 1.334 ± 0.378
0.628MetPhe: 0.628 ± 0.192
1.805MetGly: 1.805 ± 0.358
0.471MetHis: 0.471 ± 0.219
0.706MetIle: 0.706 ± 0.29
0.628MetLys: 0.628 ± 0.188
2.198MetLeu: 2.198 ± 0.364
0.549MetMet: 0.549 ± 0.174
1.413MetAsn: 1.413 ± 0.319
0.549MetPro: 0.549 ± 0.204
1.491MetGln: 1.491 ± 0.453
1.334MetArg: 1.334 ± 0.289
1.884MetSer: 1.884 ± 0.321
1.648MetThr: 1.648 ± 0.355
1.099MetVal: 1.099 ± 0.262
0.157MetTrp: 0.157 ± 0.093
0.392MetTyr: 0.392 ± 0.176
0.0MetXaa: 0.0 ± 0.0
Asn
5.181AsnAla: 5.181 ± 0.812
0.392AsnCys: 0.392 ± 0.19
2.59AsnAsp: 2.59 ± 0.367
2.669AsnGlu: 2.669 ± 0.373
1.57AsnPhe: 1.57 ± 0.37
4.553AsnGly: 4.553 ± 0.76
0.942AsnHis: 0.942 ± 0.308
1.334AsnIle: 1.334 ± 0.387
2.198AsnLys: 2.198 ± 0.386
3.689AsnLeu: 3.689 ± 0.405
0.785AsnMet: 0.785 ± 0.176
1.57AsnAsn: 1.57 ± 0.3
3.218AsnPro: 3.218 ± 0.509
1.962AsnGln: 1.962 ± 0.443
2.669AsnArg: 2.669 ± 0.373
2.512AsnSer: 2.512 ± 0.408
2.904AsnThr: 2.904 ± 0.668
2.59AsnVal: 2.59 ± 0.437
0.549AsnTrp: 0.549 ± 0.19
1.02AsnTyr: 1.02 ± 0.305
0.0AsnXaa: 0.0 ± 0.0
Pro
4.553ProAla: 4.553 ± 1.368
0.314ProCys: 0.314 ± 0.152
3.061ProAsp: 3.061 ± 0.352
3.454ProGlu: 3.454 ± 0.503
1.491ProPhe: 1.491 ± 0.375
3.689ProGly: 3.689 ± 0.562
0.471ProHis: 0.471 ± 0.198
1.962ProIle: 1.962 ± 0.284
2.433ProLys: 2.433 ± 0.452
2.747ProLeu: 2.747 ± 0.5
1.177ProMet: 1.177 ± 0.284
2.276ProAsn: 2.276 ± 0.357
1.805ProPro: 1.805 ± 0.563
1.962ProGln: 1.962 ± 0.332
1.805ProArg: 1.805 ± 0.385
3.14ProSer: 3.14 ± 0.389
2.355ProThr: 2.355 ± 0.424
2.904ProVal: 2.904 ± 0.414
0.471ProTrp: 0.471 ± 0.183
1.805ProTyr: 1.805 ± 0.349
0.0ProXaa: 0.0 ± 0.0
Gln
5.73GlnAla: 5.73 ± 1.072
0.392GlnCys: 0.392 ± 0.202
1.962GlnAsp: 1.962 ± 0.401
1.727GlnGlu: 1.727 ± 0.372
1.884GlnPhe: 1.884 ± 0.402
2.512GlnGly: 2.512 ± 0.57
1.413GlnHis: 1.413 ± 0.428
1.413GlnIle: 1.413 ± 0.339
2.59GlnLys: 2.59 ± 0.385
4.082GlnLeu: 4.082 ± 0.554
0.942GlnMet: 0.942 ± 0.309
1.334GlnAsn: 1.334 ± 0.319
1.648GlnPro: 1.648 ± 0.323
2.669GlnGln: 2.669 ± 0.545
2.826GlnArg: 2.826 ± 0.474
3.14GlnSer: 3.14 ± 0.666
2.355GlnThr: 2.355 ± 0.51
3.454GlnVal: 3.454 ± 0.468
0.863GlnTrp: 0.863 ± 0.285
1.099GlnTyr: 1.099 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
5.966ArgAla: 5.966 ± 0.489
0.392ArgCys: 0.392 ± 0.188
4.082ArgAsp: 4.082 ± 0.688
3.532ArgGlu: 3.532 ± 0.556
2.59ArgPhe: 2.59 ± 0.549
4.867ArgGly: 4.867 ± 0.621
1.256ArgHis: 1.256 ± 0.369
4.553ArgIle: 4.553 ± 0.674
3.218ArgLys: 3.218 ± 0.506
5.73ArgLeu: 5.73 ± 0.627
1.413ArgMet: 1.413 ± 0.461
2.355ArgAsn: 2.355 ± 0.504
2.747ArgPro: 2.747 ± 0.477
1.334ArgGln: 1.334 ± 0.259
4.71ArgArg: 4.71 ± 0.749
2.826ArgSer: 2.826 ± 0.512
4.003ArgThr: 4.003 ± 0.486
4.396ArgVal: 4.396 ± 0.757
1.57ArgTrp: 1.57 ± 0.418
2.198ArgTyr: 2.198 ± 0.406
0.0ArgXaa: 0.0 ± 0.0
Ser
6.044SerAla: 6.044 ± 0.944
0.549SerCys: 0.549 ± 0.204
2.904SerAsp: 2.904 ± 0.404
2.119SerGlu: 2.119 ± 0.356
1.648SerPhe: 1.648 ± 0.34
4.003SerGly: 4.003 ± 0.67
0.863SerHis: 0.863 ± 0.287
2.669SerIle: 2.669 ± 0.42
3.768SerLys: 3.768 ± 0.549
5.338SerLeu: 5.338 ± 0.974
1.648SerMet: 1.648 ± 0.383
2.983SerAsn: 2.983 ± 0.546
2.669SerPro: 2.669 ± 0.521
2.512SerGln: 2.512 ± 0.557
3.611SerArg: 3.611 ± 0.729
2.826SerSer: 2.826 ± 0.634
3.768SerThr: 3.768 ± 0.568
3.846SerVal: 3.846 ± 0.629
0.706SerTrp: 0.706 ± 0.218
2.119SerTyr: 2.119 ± 0.561
0.0SerXaa: 0.0 ± 0.0
Thr
6.123ThrAla: 6.123 ± 1.385
0.392ThrCys: 0.392 ± 0.181
2.826ThrAsp: 2.826 ± 0.543
3.846ThrGlu: 3.846 ± 0.646
1.805ThrPhe: 1.805 ± 0.39
5.338ThrGly: 5.338 ± 0.577
1.02ThrHis: 1.02 ± 0.354
2.59ThrIle: 2.59 ± 0.592
3.454ThrLys: 3.454 ± 0.691
4.71ThrLeu: 4.71 ± 0.592
1.02ThrMet: 1.02 ± 0.296
1.962ThrAsn: 1.962 ± 0.458
2.747ThrPro: 2.747 ± 0.46
2.669ThrGln: 2.669 ± 0.54
2.669ThrArg: 2.669 ± 0.402
3.14ThrSer: 3.14 ± 0.371
3.218ThrThr: 3.218 ± 0.548
3.846ThrVal: 3.846 ± 0.692
0.628ThrTrp: 0.628 ± 0.203
1.805ThrTyr: 1.805 ± 0.402
0.0ThrXaa: 0.0 ± 0.0
Val
6.594ValAla: 6.594 ± 0.686
0.471ValCys: 0.471 ± 0.175
3.768ValAsp: 3.768 ± 0.461
3.297ValGlu: 3.297 ± 0.667
2.512ValPhe: 2.512 ± 0.495
5.259ValGly: 5.259 ± 0.826
0.942ValHis: 0.942 ± 0.236
4.317ValIle: 4.317 ± 0.57
3.454ValLys: 3.454 ± 0.644
4.71ValLeu: 4.71 ± 0.688
1.727ValMet: 1.727 ± 0.35
4.317ValAsn: 4.317 ± 0.617
2.826ValPro: 2.826 ± 0.495
3.532ValGln: 3.532 ± 0.629
5.416ValArg: 5.416 ± 0.695
3.611ValSer: 3.611 ± 0.632
3.061ValThr: 3.061 ± 0.518
4.71ValVal: 4.71 ± 0.759
1.648ValTrp: 1.648 ± 0.329
2.433ValTyr: 2.433 ± 0.504
0.0ValXaa: 0.0 ± 0.0
Trp
1.648TrpAla: 1.648 ± 0.302
0.235TrpCys: 0.235 ± 0.137
1.099TrpAsp: 1.099 ± 0.297
1.334TrpGlu: 1.334 ± 0.269
0.785TrpPhe: 0.785 ± 0.261
1.02TrpGly: 1.02 ± 0.25
0.314TrpHis: 0.314 ± 0.133
0.628TrpIle: 0.628 ± 0.191
0.471TrpLys: 0.471 ± 0.15
1.884TrpLeu: 1.884 ± 0.319
0.471TrpMet: 0.471 ± 0.236
0.314TrpAsn: 0.314 ± 0.12
0.078TrpPro: 0.078 ± 0.075
0.549TrpGln: 0.549 ± 0.178
0.942TrpArg: 0.942 ± 0.248
0.863TrpSer: 0.863 ± 0.232
0.628TrpThr: 0.628 ± 0.194
1.256TrpVal: 1.256 ± 0.271
0.314TrpTrp: 0.314 ± 0.162
0.392TrpTyr: 0.392 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.59TyrAla: 2.59 ± 0.579
0.549TyrCys: 0.549 ± 0.181
1.962TyrAsp: 1.962 ± 0.407
1.727TyrGlu: 1.727 ± 0.328
1.02TyrPhe: 1.02 ± 0.218
2.59TyrGly: 2.59 ± 0.356
0.628TyrHis: 0.628 ± 0.236
1.57TyrIle: 1.57 ± 0.299
0.942TyrLys: 0.942 ± 0.247
2.904TyrLeu: 2.904 ± 0.529
0.392TyrMet: 0.392 ± 0.153
1.805TyrAsn: 1.805 ± 0.326
1.413TyrPro: 1.413 ± 0.383
1.805TyrGln: 1.805 ± 0.388
2.59TyrArg: 2.59 ± 0.483
1.805TyrSer: 1.805 ± 0.485
1.884TyrThr: 1.884 ± 0.381
2.198TyrVal: 2.198 ± 0.368
0.549TyrTrp: 0.549 ± 0.187
0.549TyrTyr: 0.549 ± 0.159
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12740 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski