Mycoplasma sp. Mirounga ES2806-NAS

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; unclassified Mycoplasma

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 606 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M4JCS7|A0A6M4JCS7_9MOLU 53EXOc domain-containing protein OS=Mycoplasma sp. Mirounga ES2806-NAS OX=754516 GN=HLA92_01345 PE=4 SV=1
MM1 pKa = 7.63QINLNDD7 pKa = 3.73KK8 pKa = 8.23TRR10 pKa = 11.84KK11 pKa = 8.2ITIQDD16 pKa = 3.68DD17 pKa = 3.56NGKK20 pKa = 8.32EE21 pKa = 4.12TEE23 pKa = 3.91ITILFDD29 pKa = 3.85FEE31 pKa = 5.43EE32 pKa = 5.01NGDD35 pKa = 3.88TYY37 pKa = 11.61LLGVLADD44 pKa = 4.21LEE46 pKa = 4.82TVVGLKK52 pKa = 9.67KK53 pKa = 10.82QNDD56 pKa = 3.61IYY58 pKa = 11.0IEE60 pKa = 4.03IPEE63 pKa = 4.55DD64 pKa = 2.84EE65 pKa = 4.27WEE67 pKa = 4.1IVEE70 pKa = 4.94EE71 pKa = 4.23VFNDD75 pKa = 3.43FLEE78 pKa = 4.42NNEE81 pKa = 4.86IITEE85 pKa = 4.09

Molecular weight: 9.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M4JBQ5|A0A6M4JBQ5_9MOLU Glycerol-3-phosphate acyltransferase OS=Mycoplasma sp. Mirounga ES2806-NAS OX=754516 GN=plsY PE=3 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84KK4 pKa = 9.14INKK7 pKa = 9.16KK8 pKa = 9.54LFVRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84PCQFCINNQEE24 pKa = 4.16YY25 pKa = 10.09IDD27 pKa = 4.26YY28 pKa = 10.48KK29 pKa = 11.0DD30 pKa = 3.96QEE32 pKa = 4.35LLQNYY37 pKa = 8.66INQHH41 pKa = 4.35GKK43 pKa = 9.44ILSSRR48 pKa = 11.84ITGCCSRR55 pKa = 11.84HH56 pKa = 4.73QRR58 pKa = 11.84TLATAIKK65 pKa = 9.74RR66 pKa = 11.84SRR68 pKa = 11.84IVAILPFIGSGKK80 pKa = 10.02VKK82 pKa = 10.72

Molecular weight: 9.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

606

0

606

232485

37

3834

383.6

44.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.883 ± 0.101

0.412 ± 0.021

5.693 ± 0.116

6.538 ± 0.094

5.367 ± 0.111

4.024 ± 0.108

1.511 ± 0.03

10.063 ± 0.142

10.43 ± 0.144

9.616 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.572 ± 0.048

8.852 ± 0.17

2.482 ± 0.048

4.074 ± 0.084

2.447 ± 0.058

6.43 ± 0.063

5.538 ± 0.075

4.883 ± 0.079

0.897 ± 0.026

4.289 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski