Amino acid dipepetide frequency for Mycoplasma sp. Mirounga ES2806-NAS

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.697AlaAla: 2.697 ± 0.13
0.232AlaCys: 0.232 ± 0.035
2.189AlaAsp: 2.189 ± 0.121
2.164AlaGlu: 2.164 ± 0.121
2.443AlaPhe: 2.443 ± 0.114
2.099AlaGly: 2.099 ± 0.113
0.723AlaHis: 0.723 ± 0.065
5.295AlaIle: 5.295 ± 0.162
5.953AlaLys: 5.953 ± 0.341
5.007AlaLeu: 5.007 ± 0.179
0.903AlaMet: 0.903 ± 0.063
3.85AlaAsn: 3.85 ± 0.177
0.912AlaPro: 0.912 ± 0.063
1.893AlaGln: 1.893 ± 0.098
1.604AlaArg: 1.604 ± 0.107
3.002AlaSer: 3.002 ± 0.142
3.093AlaThr: 3.093 ± 0.151
2.271AlaVal: 2.271 ± 0.116
0.409AlaTrp: 0.409 ± 0.049
2.09AlaTyr: 2.09 ± 0.114
0.0AlaXaa: 0.0 ± 0.0
Cys
0.301CysAla: 0.301 ± 0.033
0.03CysCys: 0.03 ± 0.011
0.211CysAsp: 0.211 ± 0.028
0.28CysGlu: 0.28 ± 0.036
0.267CysPhe: 0.267 ± 0.038
0.292CysGly: 0.292 ± 0.037
0.129CysHis: 0.129 ± 0.022
0.366CysIle: 0.366 ± 0.047
0.267CysLys: 0.267 ± 0.034
0.426CysLeu: 0.426 ± 0.045
0.052CysMet: 0.052 ± 0.015
0.249CysAsn: 0.249 ± 0.036
0.099CysPro: 0.099 ± 0.024
0.112CysGln: 0.112 ± 0.022
0.099CysArg: 0.099 ± 0.021
0.301CysSer: 0.301 ± 0.033
0.194CysThr: 0.194 ± 0.03
0.194CysVal: 0.194 ± 0.027
0.052CysTrp: 0.052 ± 0.015
0.206CysTyr: 0.206 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
2.929AspAla: 2.929 ± 0.207
0.142AspCys: 0.142 ± 0.027
3.093AspAsp: 3.093 ± 0.124
4.181AspGlu: 4.181 ± 0.182
3.777AspPhe: 3.777 ± 0.133
2.164AspGly: 2.164 ± 0.114
0.748AspHis: 0.748 ± 0.057
5.622AspIle: 5.622 ± 0.168
6.185AspLys: 6.185 ± 0.175
5.695AspLeu: 5.695 ± 0.161
0.718AspMet: 0.718 ± 0.066
4.869AspAsn: 4.869 ± 0.187
1.432AspPro: 1.432 ± 0.069
1.888AspGln: 1.888 ± 0.098
1.062AspArg: 1.062 ± 0.066
3.346AspSer: 3.346 ± 0.124
2.921AspThr: 2.921 ± 0.115
2.886AspVal: 2.886 ± 0.112
0.559AspTrp: 0.559 ± 0.055
2.71AspTyr: 2.71 ± 0.136
0.0AspXaa: 0.0 ± 0.0
Glu
3.394GluAla: 3.394 ± 0.206
0.155GluCys: 0.155 ± 0.025
3.566GluAsp: 3.566 ± 0.144
4.469GluGlu: 4.469 ± 0.175
3.579GluPhe: 3.579 ± 0.134
2.06GluGly: 2.06 ± 0.133
1.015GluHis: 1.015 ± 0.067
7.712GluIle: 7.712 ± 0.215
6.474GluLys: 6.474 ± 0.214
6.22GluLeu: 6.22 ± 0.192
1.26GluMet: 1.26 ± 0.075
6.822GluAsn: 6.822 ± 0.231
0.998GluPro: 0.998 ± 0.08
2.86GluGln: 2.86 ± 0.118
1.295GluArg: 1.295 ± 0.098
3.149GluSer: 3.149 ± 0.134
3.811GluThr: 3.811 ± 0.146
3.17GluVal: 3.17 ± 0.125
0.43GluTrp: 0.43 ± 0.045
2.938GluTyr: 2.938 ± 0.112
0.0GluXaa: 0.0 ± 0.0
Phe
2.663PheAla: 2.663 ± 0.122
0.344PheCys: 0.344 ± 0.048
3.536PheAsp: 3.536 ± 0.11
3.385PheGlu: 3.385 ± 0.142
2.628PhePhe: 2.628 ± 0.143
2.4PheGly: 2.4 ± 0.123
0.585PheHis: 0.585 ± 0.051
5.652PheIle: 5.652 ± 0.253
5.497PheLys: 5.497 ± 0.163
4.99PheLeu: 4.99 ± 0.216
0.766PheMet: 0.766 ± 0.048
4.796PheAsn: 4.796 ± 0.195
1.14PhePro: 1.14 ± 0.07
1.604PheGln: 1.604 ± 0.087
1.17PheArg: 1.17 ± 0.071
4.409PheSer: 4.409 ± 0.177
2.49PheThr: 2.49 ± 0.109
2.809PheVal: 2.809 ± 0.151
0.533PheTrp: 0.533 ± 0.049
2.275PheTyr: 2.275 ± 0.111
0.0PheXaa: 0.0 ± 0.0
Gly
2.181GlyAla: 2.181 ± 0.117
0.172GlyCys: 0.172 ± 0.027
2.121GlyAsp: 2.121 ± 0.09
2.133GlyGlu: 2.133 ± 0.118
2.697GlyPhe: 2.697 ± 0.131
2.426GlyGly: 2.426 ± 0.152
0.834GlyHis: 0.834 ± 0.066
4.245GlyIle: 4.245 ± 0.153
3.536GlyLys: 3.536 ± 0.16
3.682GlyLeu: 3.682 ± 0.143
0.791GlyMet: 0.791 ± 0.066
2.267GlyAsn: 2.267 ± 0.11
0.895GlyPro: 0.895 ± 0.07
1.566GlyGln: 1.566 ± 0.092
1.269GlyArg: 1.269 ± 0.094
2.706GlySer: 2.706 ± 0.123
2.241GlyThr: 2.241 ± 0.124
2.103GlyVal: 2.103 ± 0.104
0.456GlyTrp: 0.456 ± 0.054
1.918GlyTyr: 1.918 ± 0.103
0.0GlyXaa: 0.0 ± 0.0
His
0.753HisAla: 0.753 ± 0.052
0.077HisCys: 0.077 ± 0.02
0.736HisAsp: 0.736 ± 0.053
0.817HisGlu: 0.817 ± 0.062
0.895HisPhe: 0.895 ± 0.071
0.705HisGly: 0.705 ± 0.062
0.357HisHis: 0.357 ± 0.042
1.445HisIle: 1.445 ± 0.084
1.488HisLys: 1.488 ± 0.076
1.415HisLeu: 1.415 ± 0.075
0.219HisMet: 0.219 ± 0.034
1.299HisAsn: 1.299 ± 0.087
0.568HisPro: 0.568 ± 0.047
0.624HisGln: 0.624 ± 0.055
0.37HisArg: 0.37 ± 0.035
1.127HisSer: 1.127 ± 0.067
0.787HisThr: 0.787 ± 0.06
0.598HisVal: 0.598 ± 0.054
0.146HisTrp: 0.146 ± 0.024
0.684HisTyr: 0.684 ± 0.062
0.0HisXaa: 0.0 ± 0.0
Ile
5.054IleAla: 5.054 ± 0.156
0.469IleCys: 0.469 ± 0.054
5.996IleAsp: 5.996 ± 0.156
6.465IleGlu: 6.465 ± 0.185
5.63IlePhe: 5.63 ± 0.243
3.867IleGly: 3.867 ± 0.168
1.475IleHis: 1.475 ± 0.08
9.377IleIle: 9.377 ± 0.327
10.59IleLys: 10.59 ± 0.29
9.45IleLeu: 9.45 ± 0.318
1.299IleMet: 1.299 ± 0.079
9.36IleAsn: 9.36 ± 0.291
2.869IlePro: 2.869 ± 0.121
3.635IleGln: 3.635 ± 0.12
2.456IleArg: 2.456 ± 0.125
7.325IleSer: 7.325 ± 0.219
5.411IleThr: 5.411 ± 0.154
5.089IleVal: 5.089 ± 0.17
0.731IleTrp: 0.731 ± 0.062
4.078IleTyr: 4.078 ± 0.139
0.0IleXaa: 0.0 ± 0.0
Lys
4.495LysAla: 4.495 ± 0.243
0.262LysCys: 0.262 ± 0.033
6.155LysAsp: 6.155 ± 0.23
7.652LysGlu: 7.652 ± 0.212
4.37LysPhe: 4.37 ± 0.14
3.045LysGly: 3.045 ± 0.141
1.583LysHis: 1.583 ± 0.093
11.136LysIle: 11.136 ± 0.222
10.009LysLys: 10.009 ± 0.259
9.738LysLeu: 9.738 ± 0.248
2.387LysMet: 2.387 ± 0.089
10.448LysAsn: 10.448 ± 0.275
2.254LysPro: 2.254 ± 0.107
5.243LysGln: 5.243 ± 0.184
2.486LysArg: 2.486 ± 0.105
5.596LysSer: 5.596 ± 0.121
6.254LysThr: 6.254 ± 0.199
4.624LysVal: 4.624 ± 0.128
1.11LysTrp: 1.11 ± 0.083
5.446LysTyr: 5.446 ± 0.204
0.0LysXaa: 0.0 ± 0.0
Leu
5.076LeuAla: 5.076 ± 0.169
0.361LeuCys: 0.361 ± 0.04
6.043LeuAsp: 6.043 ± 0.173
6.792LeuGlu: 6.792 ± 0.232
4.762LeuPhe: 4.762 ± 0.197
3.974LeuGly: 3.974 ± 0.154
1.454LeuHis: 1.454 ± 0.071
9.166LeuIle: 9.166 ± 0.258
10.874LeuLys: 10.874 ± 0.266
8.233LeuLeu: 8.233 ± 0.251
1.544LeuMet: 1.544 ± 0.096
8.865LeuAsn: 8.865 ± 0.301
2.62LeuPro: 2.62 ± 0.162
3.514LeuGln: 3.514 ± 0.146
2.443LeuArg: 2.443 ± 0.13
6.585LeuSer: 6.585 ± 0.188
5.308LeuThr: 5.308 ± 0.174
4.684LeuVal: 4.684 ± 0.143
0.718LeuTrp: 0.718 ± 0.059
3.14LeuTyr: 3.14 ± 0.127
0.0LeuXaa: 0.0 ± 0.0
Met
0.813MetAla: 0.813 ± 0.072
0.082MetCys: 0.082 ± 0.021
0.783MetAsp: 0.783 ± 0.069
0.976MetGlu: 0.976 ± 0.074
0.985MetPhe: 0.985 ± 0.062
0.611MetGly: 0.611 ± 0.057
0.249MetHis: 0.249 ± 0.033
1.51MetIle: 1.51 ± 0.088
1.764MetLys: 1.764 ± 0.082
1.376MetLeu: 1.376 ± 0.085
0.31MetMet: 0.31 ± 0.037
1.131MetAsn: 1.131 ± 0.065
0.508MetPro: 0.508 ± 0.049
0.705MetGln: 0.705 ± 0.061
0.503MetArg: 0.503 ± 0.045
1.196MetSer: 1.196 ± 0.071
0.757MetThr: 0.757 ± 0.061
0.761MetVal: 0.761 ± 0.055
0.138MetTrp: 0.138 ± 0.026
0.563MetTyr: 0.563 ± 0.058
0.0MetXaa: 0.0 ± 0.0
Asn
3.729AsnAla: 3.729 ± 0.161
0.348AsnCys: 0.348 ± 0.064
5.385AsnAsp: 5.385 ± 0.218
5.837AsnGlu: 5.837 ± 0.173
4.392AsnPhe: 4.392 ± 0.177
2.856AsnGly: 2.856 ± 0.126
1.118AsnHis: 1.118 ± 0.065
9.067AsnIle: 9.067 ± 0.31
9.786AsnLys: 9.786 ± 0.296
9.011AsnLeu: 9.011 ± 0.283
1.114AsnMet: 1.114 ± 0.071
8.955AsnAsn: 8.955 ± 0.353
2.542AsnPro: 2.542 ± 0.108
3.893AsnGln: 3.893 ± 0.189
1.729AsnArg: 1.729 ± 0.088
5.398AsnSer: 5.398 ± 0.182
4.297AsnThr: 4.297 ± 0.17
4.25AsnVal: 4.25 ± 0.134
0.908AsnTrp: 0.908 ± 0.073
3.901AsnTyr: 3.901 ± 0.135
0.0AsnXaa: 0.0 ± 0.0
Pro
1.101ProAla: 1.101 ± 0.08
0.103ProCys: 0.103 ± 0.023
1.243ProAsp: 1.243 ± 0.085
1.97ProGlu: 1.97 ± 0.09
1.394ProPhe: 1.394 ± 0.08
1.105ProGly: 1.105 ± 0.072
0.529ProHis: 0.529 ± 0.056
2.232ProIle: 2.232 ± 0.112
2.336ProLys: 2.336 ± 0.118
2.327ProLeu: 2.327 ± 0.102
0.323ProMet: 0.323 ± 0.041
2.108ProAsn: 2.108 ± 0.104
0.443ProPro: 0.443 ± 0.04
1.032ProGln: 1.032 ± 0.065
0.551ProArg: 0.551 ± 0.045
1.746ProSer: 1.746 ± 0.092
1.622ProThr: 1.622 ± 0.101
1.381ProVal: 1.381 ± 0.085
0.258ProTrp: 0.258 ± 0.032
1.015ProTyr: 1.015 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
2.09GlnAla: 2.09 ± 0.136
0.103GlnCys: 0.103 ± 0.021
2.116GlnAsp: 2.116 ± 0.107
2.955GlnGlu: 2.955 ± 0.143
1.695GlnPhe: 1.695 ± 0.093
1.346GlnGly: 1.346 ± 0.064
0.581GlnHis: 0.581 ± 0.054
4.048GlnIle: 4.048 ± 0.165
5.218GlnLys: 5.218 ± 0.222
3.626GlnLeu: 3.626 ± 0.125
0.701GlnMet: 0.701 ± 0.057
4.155GlnAsn: 4.155 ± 0.196
0.899GlnPro: 0.899 ± 0.069
1.914GlnGln: 1.914 ± 0.109
1.148GlnArg: 1.148 ± 0.084
2.078GlnSer: 2.078 ± 0.112
2.688GlnThr: 2.688 ± 0.12
1.544GlnVal: 1.544 ± 0.081
0.379GlnTrp: 0.379 ± 0.043
1.454GlnTyr: 1.454 ± 0.087
0.0GlnXaa: 0.0 ± 0.0
Arg
1.303ArgAla: 1.303 ± 0.086
0.129ArgCys: 0.129 ± 0.021
1.376ArgAsp: 1.376 ± 0.086
1.755ArgGlu: 1.755 ± 0.099
1.295ArgPhe: 1.295 ± 0.091
1.234ArgGly: 1.234 ± 0.078
0.353ArgHis: 0.353 ± 0.039
2.258ArgIle: 2.258 ± 0.11
2.275ArgLys: 2.275 ± 0.122
2.254ArgLeu: 2.254 ± 0.101
0.508ArgMet: 0.508 ± 0.044
1.764ArgAsn: 1.764 ± 0.087
0.71ArgPro: 0.71 ± 0.055
1.213ArgGln: 1.213 ± 0.077
0.89ArgArg: 0.89 ± 0.069
1.527ArgSer: 1.527 ± 0.084
1.08ArgThr: 1.08 ± 0.063
1.325ArgVal: 1.325 ± 0.096
0.151ArgTrp: 0.151 ± 0.028
1.071ArgTyr: 1.071 ± 0.076
0.0ArgXaa: 0.0 ± 0.0
Ser
2.83SerAla: 2.83 ± 0.118
0.409SerCys: 0.409 ± 0.043
3.428SerAsp: 3.428 ± 0.124
3.936SerGlu: 3.936 ± 0.134
3.815SerPhe: 3.815 ± 0.15
3.054SerGly: 3.054 ± 0.152
1.007SerHis: 1.007 ± 0.076
6.314SerIle: 6.314 ± 0.215
6.426SerLys: 6.426 ± 0.178
6.805SerLeu: 6.805 ± 0.193
0.869SerMet: 0.869 ± 0.055
4.59SerAsn: 4.59 ± 0.152
1.6SerPro: 1.6 ± 0.09
2.921SerGln: 2.921 ± 0.119
1.561SerArg: 1.561 ± 0.086
4.31SerSer: 4.31 ± 0.147
3.682SerThr: 3.682 ± 0.149
3.389SerVal: 3.389 ± 0.119
0.499SerTrp: 0.499 ± 0.043
2.852SerTyr: 2.852 ± 0.105
0.0SerXaa: 0.0 ± 0.0
Thr
2.202ThrAla: 2.202 ± 0.11
0.206ThrCys: 0.206 ± 0.031
2.667ThrAsp: 2.667 ± 0.098
3.088ThrGlu: 3.088 ± 0.142
3.205ThrPhe: 3.205 ± 0.151
2.37ThrGly: 2.37 ± 0.116
1.062ThrHis: 1.062 ± 0.062
5.76ThrIle: 5.76 ± 0.161
6.048ThrLys: 6.048 ± 0.178
5.536ThrLeu: 5.536 ± 0.155
0.654ThrMet: 0.654 ± 0.044
5.222ThrAsn: 5.222 ± 0.204
1.669ThrPro: 1.669 ± 0.086
2.443ThrGln: 2.443 ± 0.108
1.359ThrArg: 1.359 ± 0.081
3.6ThrSer: 3.6 ± 0.166
3.308ThrThr: 3.308 ± 0.123
2.22ThrVal: 2.22 ± 0.116
0.46ThrTrp: 0.46 ± 0.052
2.301ThrTyr: 2.301 ± 0.09
0.0ThrXaa: 0.0 ± 0.0
Val
2.856ValAla: 2.856 ± 0.123
0.232ValCys: 0.232 ± 0.035
3.063ValAsp: 3.063 ± 0.125
3.2ValGlu: 3.2 ± 0.138
2.835ValPhe: 2.835 ± 0.131
2.49ValGly: 2.49 ± 0.121
0.68ValHis: 0.68 ± 0.06
4.783ValIle: 4.783 ± 0.162
4.013ValLys: 4.013 ± 0.139
4.71ValLeu: 4.71 ± 0.174
0.65ValMet: 0.65 ± 0.058
3.648ValAsn: 3.648 ± 0.135
1.338ValPro: 1.338 ± 0.094
1.454ValGln: 1.454 ± 0.083
1.032ValArg: 1.032 ± 0.083
3.648ValSer: 3.648 ± 0.158
2.8ValThr: 2.8 ± 0.126
2.921ValVal: 2.921 ± 0.134
0.46ValTrp: 0.46 ± 0.05
2.022ValTyr: 2.022 ± 0.099
0.0ValXaa: 0.0 ± 0.0
Trp
0.366TrpAla: 0.366 ± 0.043
0.039TrpCys: 0.039 ± 0.013
0.482TrpAsp: 0.482 ± 0.048
0.512TrpGlu: 0.512 ± 0.058
0.559TrpPhe: 0.559 ± 0.051
0.379TrpGly: 0.379 ± 0.038
0.082TrpHis: 0.082 ± 0.021
1.019TrpIle: 1.019 ± 0.081
0.951TrpLys: 0.951 ± 0.069
0.847TrpLeu: 0.847 ± 0.066
0.219TrpMet: 0.219 ± 0.033
0.847TrpAsn: 0.847 ± 0.057
0.219TrpPro: 0.219 ± 0.028
0.331TrpGln: 0.331 ± 0.039
0.237TrpArg: 0.237 ± 0.035
0.447TrpSer: 0.447 ± 0.055
0.49TrpThr: 0.49 ± 0.049
0.46TrpVal: 0.46 ± 0.044
0.099TrpTrp: 0.099 ± 0.025
0.387TrpTyr: 0.387 ± 0.043
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.996TyrAla: 1.996 ± 0.107
0.228TyrCys: 0.228 ± 0.034
2.74TyrAsp: 2.74 ± 0.15
2.809TyrGlu: 2.809 ± 0.126
2.452TyrPhe: 2.452 ± 0.121
1.759TyrGly: 1.759 ± 0.097
0.546TyrHis: 0.546 ± 0.048
3.617TyrIle: 3.617 ± 0.123
4.611TyrLys: 4.611 ± 0.155
4.809TyrLeu: 4.809 ± 0.188
0.43TyrMet: 0.43 ± 0.044
3.273TyrAsn: 3.273 ± 0.126
1.105TyrPro: 1.105 ± 0.077
1.893TyrGln: 1.893 ± 0.108
1.204TyrArg: 1.204 ± 0.072
2.8TyrSer: 2.8 ± 0.123
2.146TyrThr: 2.146 ± 0.086
2.155TyrVal: 2.155 ± 0.092
0.477TyrTrp: 0.477 ± 0.046
1.837TyrTyr: 1.837 ± 0.096
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 606 proteins (232485 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski