Rotavirus A (isolate RVA/Monkey/South Africa/SA11-H96/1958/G3P5B[2]) (RV-A) (Simian Agent 11 (isolate SI/South Africa/H96/58))

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A; Simian rotavirus A; Simian rotavirus A/SA11

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A2T3P5-2|VP7-2_ROTSH Isoform of A2T3P5 Isoform 2 of Outer capsid glycoprotein VP7 OS=Rotavirus A (isolate RVA/Monkey/South Africa/SA11-H96/1958/G3P5B[2]) OX=450149 PE=1 SV=1
MM1 pKa = 7.9DD2 pKa = 4.82FIIYY6 pKa = 10.0RR7 pKa = 11.84FLFIIVILSPFLRR20 pKa = 11.84AQNYY24 pKa = 8.58GINLPITGSMDD35 pKa = 3.06TAYY38 pKa = 10.65ANSTQEE44 pKa = 4.12EE45 pKa = 4.88TFLTSTLCLYY55 pKa = 10.75YY56 pKa = 8.86PTEE59 pKa = 4.27AATEE63 pKa = 4.11INDD66 pKa = 3.85NSWKK70 pKa = 9.38DD71 pKa = 3.5TLSQLFLTKK80 pKa = 10.05GWPTGSVYY88 pKa = 10.48FKK90 pKa = 10.03EE91 pKa = 4.3YY92 pKa = 10.64TNIASFSVDD101 pKa = 3.03PQLYY105 pKa = 9.31CDD107 pKa = 3.92YY108 pKa = 11.0NVVLMKK114 pKa = 10.53YY115 pKa = 10.24DD116 pKa = 3.35ATLQLDD122 pKa = 3.72MSEE125 pKa = 4.24LADD128 pKa = 5.13LILNEE133 pKa = 4.24WLCNPMDD140 pKa = 3.29ITLYY144 pKa = 10.67YY145 pKa = 9.25YY146 pKa = 10.55QQTDD150 pKa = 3.57EE151 pKa = 4.17ANKK154 pKa = 8.08WISMGSSCTIKK165 pKa = 10.46VCPLNTQTLGIGCLTTDD182 pKa = 2.57ATTFEE187 pKa = 4.44EE188 pKa = 4.7VATAEE193 pKa = 4.34KK194 pKa = 10.74LVITDD199 pKa = 3.22VVDD202 pKa = 4.31GVNHH206 pKa = 6.65KK207 pKa = 10.85LDD209 pKa = 3.69VTTATCTIRR218 pKa = 11.84NCKK221 pKa = 9.83KK222 pKa = 10.18LGPRR226 pKa = 11.84EE227 pKa = 4.01NVAVIQVGGSDD238 pKa = 3.65ILDD241 pKa = 3.33ITADD245 pKa = 3.74PTTAPQTEE253 pKa = 3.66RR254 pKa = 11.84MMRR257 pKa = 11.84INWKK261 pKa = 9.54KK262 pKa = 7.26WWQVFYY268 pKa = 10.57TVVDD272 pKa = 3.76YY273 pKa = 11.33VDD275 pKa = 4.77QIIQVMSKK283 pKa = 10.16RR284 pKa = 11.84SRR286 pKa = 11.84SLNSAAFYY294 pKa = 11.0YY295 pKa = 10.27RR296 pKa = 11.84VV297 pKa = 3.19

Molecular weight: 33.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A2T3R5|VP2_ROTSH Inner capsid protein VP2 OS=Rotavirus A (isolate RVA/Monkey/South Africa/SA11-H96/1958/G3P5B[2]) OX=450149 PE=1 SV=1
MM1 pKa = 7.42NRR3 pKa = 11.84LQQRR7 pKa = 11.84QLFLEE12 pKa = 4.16NLLVGVNSTFHH23 pKa = 6.05QMQKK27 pKa = 10.78HH28 pKa = 6.44SINTCCRR35 pKa = 11.84SLQRR39 pKa = 11.84ILDD42 pKa = 3.95HH43 pKa = 7.28LILLQTIHH51 pKa = 6.7SPVFRR56 pKa = 11.84LDD58 pKa = 3.41RR59 pKa = 11.84MQLRR63 pKa = 11.84QMQTLACLWIHH74 pKa = 5.54QHH76 pKa = 5.34NHH78 pKa = 6.38DD79 pKa = 4.51LQVMSDD85 pKa = 4.07AIKK88 pKa = 10.03WISPP92 pKa = 3.47

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

1

13

6194

92

1088

476.5

54.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.344 ± 0.37

1.195 ± 0.321

6.087 ± 0.253

5.425 ± 0.293

4.391 ± 0.258

3.407 ± 0.313

1.55 ± 0.255

8.056 ± 0.325

6.345 ± 0.602

9.509 ± 0.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.003 ± 0.246

6.991 ± 0.318

3.374 ± 0.308

4.294 ± 0.449

4.682 ± 0.297

7.588 ± 0.716

6.619 ± 0.626

6.006 ± 0.292

1.162 ± 0.137

4.973 ± 0.521
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski