Amino acid dipepetide frequency for Roseobacter sp. AzwK-3b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.666AlaAla: 15.666 ± 0.182	1.16AlaCys: 1.16 ± 0.035	7.039AlaAsp: 7.039 ± 0.092	7.836AlaGlu: 7.836 ± 0.101	4.238AlaPhe: 4.238 ± 0.06	10.36AlaGly: 10.36 ± 0.136	2.525AlaHis: 2.525 ± 0.053	5.797AlaIle: 5.797 ± 0.073	3.511AlaLys: 3.511 ± 0.071	13.46AlaLeu: 13.46 ± 0.147	3.842AlaMet: 3.842 ± 0.067	2.752AlaAsn: 2.752 ± 0.061	5.801AlaPro: 5.801 ± 0.1	4.725AlaGln: 4.725 ± 0.073	9.233AlaArg: 9.233 ± 0.11	5.556AlaSer: 5.556 ± 0.075	5.86AlaThr: 5.86 ± 0.089	8.241AlaVal: 8.241 ± 0.103	1.397AlaTrp: 1.397 ± 0.035	2.468AlaTyr: 2.468 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.138CysAla: 1.138 ± 0.032	0.126CysCys: 0.126 ± 0.01	0.662CysAsp: 0.662 ± 0.023	0.46CysGlu: 0.46 ± 0.021	0.354CysPhe: 0.354 ± 0.017	0.981CysGly: 0.981 ± 0.034	0.267CysHis: 0.267 ± 0.014	0.419CysIle: 0.419 ± 0.017	0.221CysLys: 0.221 ± 0.013	0.888CysLeu: 0.888 ± 0.029	0.188CysMet: 0.188 ± 0.013	0.238CysAsn: 0.238 ± 0.015	0.507CysPro: 0.507 ± 0.024	0.26CysGln: 0.26 ± 0.014	0.6CysArg: 0.6 ± 0.023	0.463CysSer: 0.463 ± 0.021	0.473CysThr: 0.473 ± 0.023	0.63CysVal: 0.63 ± 0.026	0.135CysTrp: 0.135 ± 0.012	0.235CysTyr: 0.235 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	7.758AspAla: 7.758 ± 0.095	0.534AspCys: 0.534 ± 0.024	3.664AspAsp: 3.664 ± 0.093	3.631AspGlu: 3.631 ± 0.068	2.161AspPhe: 2.161 ± 0.045	5.63AspGly: 5.63 ± 0.121	1.475AspHis: 1.475 ± 0.045	3.554AspIle: 3.554 ± 0.063	1.667AspLys: 1.667 ± 0.042	6.688AspLeu: 6.688 ± 0.094	1.897AspMet: 1.897 ± 0.043	1.38AspAsn: 1.38 ± 0.041	3.721AspPro: 3.721 ± 0.064	1.985AspGln: 1.985 ± 0.044	4.625AspArg: 4.625 ± 0.064	2.274AspSer: 2.274 ± 0.049	3.363AspThr: 3.363 ± 0.071	4.495AspVal: 4.495 ± 0.083	1.236AspTrp: 1.236 ± 0.036	1.521AspTyr: 1.521 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	8.198GluAla: 8.198 ± 0.102	0.381GluCys: 0.381 ± 0.017	3.538GluAsp: 3.538 ± 0.062	3.342GluGlu: 3.342 ± 0.081	1.841GluPhe: 1.841 ± 0.044	4.83GluGly: 4.83 ± 0.08	1.186GluHis: 1.186 ± 0.033	3.677GluIle: 3.677 ± 0.059	2.06GluLys: 2.06 ± 0.049	5.071GluLeu: 5.071 ± 0.082	1.884GluMet: 1.884 ± 0.04	1.694GluAsn: 1.694 ± 0.039	2.505GluPro: 2.505 ± 0.064	2.12GluGln: 2.12 ± 0.046	4.414GluArg: 4.414 ± 0.07	2.05GluSer: 2.05 ± 0.039	3.97GluThr: 3.97 ± 0.064	4.039GluVal: 4.039 ± 0.065	0.728GluTrp: 0.728 ± 0.025	1.113GluTyr: 1.113 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.157PheAla: 4.157 ± 0.065	0.433PheCys: 0.433 ± 0.018	2.828PheAsp: 2.828 ± 0.047	2.2PheGlu: 2.2 ± 0.039	1.417PhePhe: 1.417 ± 0.037	3.566PheGly: 3.566 ± 0.053	0.764PheHis: 0.764 ± 0.025	1.684PheIle: 1.684 ± 0.04	0.898PheLys: 0.898 ± 0.027	3.327PheLeu: 3.327 ± 0.064	0.902PheMet: 0.902 ± 0.026	1.025PheAsn: 1.025 ± 0.027	1.458PhePro: 1.458 ± 0.036	1.047PheGln: 1.047 ± 0.027	2.172PheArg: 2.172 ± 0.046	2.076PheSer: 2.076 ± 0.044	2.008PheThr: 2.008 ± 0.046	2.651PheVal: 2.651 ± 0.056	0.578PheTrp: 0.578 ± 0.022	0.883PheTyr: 0.883 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	9.845GlyAla: 9.845 ± 0.129	0.897GlyCys: 0.897 ± 0.032	5.043GlyAsp: 5.043 ± 0.13	4.467GlyGlu: 4.467 ± 0.072	3.56GlyPhe: 3.56 ± 0.058	7.425GlyGly: 7.425 ± 0.131	2.065GlyHis: 2.065 ± 0.05	4.464GlyIle: 4.464 ± 0.08	2.975GlyLys: 2.975 ± 0.062	9.388GlyLeu: 9.388 ± 0.103	2.623GlyMet: 2.623 ± 0.054	2.131GlyAsn: 2.131 ± 0.084	3.716GlyPro: 3.716 ± 0.06	3.337GlyGln: 3.337 ± 0.053	5.828GlyArg: 5.828 ± 0.072	4.15GlySer: 4.15 ± 0.078	4.71GlyThr: 4.71 ± 0.124	6.196GlyVal: 6.196 ± 0.088	1.455GlyTrp: 1.455 ± 0.039	2.329GlyTyr: 2.329 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.433HisAla: 2.433 ± 0.05	0.243HisCys: 0.243 ± 0.016	1.48HisAsp: 1.48 ± 0.05	1.205HisGlu: 1.205 ± 0.035	0.813HisPhe: 0.813 ± 0.026	2.026HisGly: 2.026 ± 0.046	0.589HisHis: 0.589 ± 0.026	0.987HisIle: 0.987 ± 0.031	0.535HisLys: 0.535 ± 0.019	2.191HisLeu: 2.191 ± 0.046	0.583HisMet: 0.583 ± 0.023	0.493HisAsn: 0.493 ± 0.022	1.373HisPro: 1.373 ± 0.039	0.637HisGln: 0.637 ± 0.026	1.409HisArg: 1.409 ± 0.037	0.992HisSer: 0.992 ± 0.031	0.837HisThr: 0.837 ± 0.025	1.642HisVal: 1.642 ± 0.043	0.378HisTrp: 0.378 ± 0.018	0.536HisTyr: 0.536 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.867IleAla: 6.867 ± 0.082	0.642IleCys: 0.642 ± 0.025	3.605IleAsp: 3.605 ± 0.059	3.761IleGlu: 3.761 ± 0.062	1.745IlePhe: 1.745 ± 0.043	4.918IleGly: 4.918 ± 0.079	0.933IleHis: 0.933 ± 0.032	2.17IleIle: 2.17 ± 0.046	1.477IleLys: 1.477 ± 0.039	4.968IleLeu: 4.968 ± 0.066	1.149IleMet: 1.149 ± 0.036	1.403IleAsn: 1.403 ± 0.035	2.324IlePro: 2.324 ± 0.045	1.191IleGln: 1.191 ± 0.033	3.294IleArg: 3.294 ± 0.049	2.997IleSer: 2.997 ± 0.053	3.13IleThr: 3.13 ± 0.061	3.761IleVal: 3.761 ± 0.065	0.712IleTrp: 0.712 ± 0.023	1.175IleTyr: 1.175 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.822LysAla: 3.822 ± 0.07	0.194LysCys: 0.194 ± 0.013	1.695LysAsp: 1.695 ± 0.047	1.452LysGlu: 1.452 ± 0.043	0.857LysPhe: 0.857 ± 0.031	2.576LysGly: 2.576 ± 0.058	0.595LysHis: 0.595 ± 0.022	1.553LysIle: 1.553 ± 0.041	1.128LysLys: 1.128 ± 0.041	2.964LysLeu: 2.964 ± 0.064	0.831LysMet: 0.831 ± 0.031	0.707LysAsn: 0.707 ± 0.028	1.77LysPro: 1.77 ± 0.045	0.894LysGln: 0.894 ± 0.032	2.26LysArg: 2.26 ± 0.05	1.759LysSer: 1.759 ± 0.044	1.949LysThr: 1.949 ± 0.049	2.152LysVal: 2.152 ± 0.05	0.36LysTrp: 0.36 ± 0.019	0.622LysTyr: 0.622 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	12.533LeuAla: 12.533 ± 0.119	1.009LeuCys: 1.009 ± 0.03	6.32LeuAsp: 6.32 ± 0.078	5.664LeuGlu: 5.664 ± 0.083	3.43LeuPhe: 3.43 ± 0.063	8.143LeuGly: 8.143 ± 0.1	1.975LeuHis: 1.975 ± 0.046	5.166LeuIle: 5.166 ± 0.079	3.071LeuLys: 3.071 ± 0.065	8.783LeuLeu: 8.783 ± 0.121	2.761LeuMet: 2.761 ± 0.055	2.674LeuAsn: 2.674 ± 0.045	5.398LeuPro: 5.398 ± 0.079	3.039LeuGln: 3.039 ± 0.056	7.285LeuArg: 7.285 ± 0.075	6.778LeuSer: 6.778 ± 0.075	5.88LeuThr: 5.88 ± 0.089	6.956LeuVal: 6.956 ± 0.081	1.311LeuTrp: 1.311 ± 0.041	1.974LeuTyr: 1.974 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.616MetAla: 3.616 ± 0.063	0.213MetCys: 0.213 ± 0.015	1.535MetAsp: 1.535 ± 0.03	1.354MetGlu: 1.354 ± 0.04	0.851MetPhe: 0.851 ± 0.03	2.36MetGly: 2.36 ± 0.045	0.493MetHis: 0.493 ± 0.021	1.652MetIle: 1.652 ± 0.037	0.978MetLys: 0.978 ± 0.027	2.688MetLeu: 2.688 ± 0.052	0.837MetMet: 0.837 ± 0.025	0.755MetAsn: 0.755 ± 0.025	1.566MetPro: 1.566 ± 0.037	1.088MetGln: 1.088 ± 0.03	2.065MetArg: 2.065 ± 0.047	1.771MetSer: 1.771 ± 0.042	2.165MetThr: 2.165 ± 0.046	1.857MetVal: 1.857 ± 0.044	0.241MetTrp: 0.241 ± 0.015	0.357MetTyr: 0.357 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.099AsnAla: 3.099 ± 0.06	0.254AsnCys: 0.254 ± 0.016	1.557AsnAsp: 1.557 ± 0.07	1.265AsnGlu: 1.265 ± 0.034	0.885AsnPhe: 0.885 ± 0.03	2.347AsnGly: 2.347 ± 0.064	0.48AsnHis: 0.48 ± 0.021	1.355AsnIle: 1.355 ± 0.032	0.656AsnLys: 0.656 ± 0.025	2.474AsnLeu: 2.474 ± 0.046	0.716AsnMet: 0.716 ± 0.024	0.666AsnAsn: 0.666 ± 0.03	1.814AsnPro: 1.814 ± 0.035	0.678AsnGln: 0.678 ± 0.027	1.714AsnArg: 1.714 ± 0.041	1.167AsnSer: 1.167 ± 0.038	1.404AsnThr: 1.404 ± 0.043	1.83AsnVal: 1.83 ± 0.044	0.414AsnTrp: 0.414 ± 0.019	0.606AsnTyr: 0.606 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	5.37ProAla: 5.37 ± 0.073	0.382ProCys: 0.382 ± 0.019	4.178ProAsp: 4.178 ± 0.071	4.155ProGlu: 4.155 ± 0.074	1.907ProPhe: 1.907 ± 0.041	4.659ProGly: 4.659 ± 0.065	1.091ProHis: 1.091 ± 0.034	2.247ProIle: 2.247 ± 0.046	1.539ProLys: 1.539 ± 0.04	4.485ProLeu: 4.485 ± 0.065	1.274ProMet: 1.274 ± 0.037	1.252ProAsn: 1.252 ± 0.035	2.215ProPro: 2.215 ± 0.046	1.715ProGln: 1.715 ± 0.044	2.932ProArg: 2.932 ± 0.055	2.472ProSer: 2.472 ± 0.045	2.314ProThr: 2.314 ± 0.039	4.288ProVal: 4.288 ± 0.058	0.633ProTrp: 0.633 ± 0.024	1.118ProTyr: 1.118 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.409GlnAla: 4.409 ± 0.07	0.218GlnCys: 0.218 ± 0.013	1.866GlnAsp: 1.866 ± 0.035	1.646GlnGlu: 1.646 ± 0.035	1.086GlnPhe: 1.086 ± 0.029	2.741GlnGly: 2.741 ± 0.049	0.635GlnHis: 0.635 ± 0.025	2.053GlnIle: 2.053 ± 0.043	1.115GlnLys: 1.115 ± 0.037	2.872GlnLeu: 2.872 ± 0.054	1.136GlnMet: 1.136 ± 0.032	0.905GlnAsn: 0.905 ± 0.033	1.686GlnPro: 1.686 ± 0.041	1.213GlnGln: 1.213 ± 0.039	2.251GlnArg: 2.251 ± 0.047	1.86GlnSer: 1.86 ± 0.035	2.051GlnThr: 2.051 ± 0.049	2.403GlnVal: 2.403 ± 0.048	0.413GlnTrp: 0.413 ± 0.016	0.603GlnTyr: 0.603 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.357ArgAla: 8.357 ± 0.104	0.528ArgCys: 0.528 ± 0.021	4.825ArgAsp: 4.825 ± 0.072	3.933ArgGlu: 3.933 ± 0.065	2.637ArgPhe: 2.637 ± 0.053	4.624ArgGly: 4.624 ± 0.06	1.792ArgHis: 1.792 ± 0.036	4.051ArgIle: 4.051 ± 0.063	2.413ArgLys: 2.413 ± 0.055	7.629ArgLeu: 7.629 ± 0.098	2.061ArgMet: 2.061 ± 0.047	1.814ArgAsn: 1.814 ± 0.038	3.351ArgPro: 3.351 ± 0.056	2.498ArgGln: 2.498 ± 0.046	5.324ArgArg: 5.324 ± 0.082	3.305ArgSer: 3.305 ± 0.06	3.047ArgThr: 3.047 ± 0.054	4.799ArgVal: 4.799 ± 0.066	0.952ArgTrp: 0.952 ± 0.027	1.568ArgTyr: 1.568 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	5.731SerAla: 5.731 ± 0.077	0.462SerCys: 0.462 ± 0.023	3.576SerAsp: 3.576 ± 0.067	2.953SerGlu: 2.953 ± 0.051	2.124SerPhe: 2.124 ± 0.048	5.412SerGly: 5.412 ± 0.092	1.077SerHis: 1.077 ± 0.037	2.442SerIle: 2.442 ± 0.048	1.42SerLys: 1.42 ± 0.039	5.049SerLeu: 5.049 ± 0.07	1.391SerMet: 1.391 ± 0.034	1.299SerAsn: 1.299 ± 0.034	2.487SerPro: 2.487 ± 0.047	1.64SerGln: 1.64 ± 0.038	3.321SerArg: 3.321 ± 0.054	2.587SerSer: 2.587 ± 0.052	2.501SerThr: 2.501 ± 0.051	3.768SerVal: 3.768 ± 0.063	0.66SerTrp: 0.66 ± 0.023	1.307SerTyr: 1.307 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.209ThrAla: 6.209 ± 0.112	0.515ThrCys: 0.515 ± 0.022	3.295ThrAsp: 3.295 ± 0.064	3.057ThrGlu: 3.057 ± 0.05	1.957ThrPhe: 1.957 ± 0.046	5.698ThrGly: 5.698 ± 0.095	1.131ThrHis: 1.131 ± 0.032	2.811ThrIle: 2.811 ± 0.065	1.415ThrLys: 1.415 ± 0.036	6.059ThrLeu: 6.059 ± 0.083	1.27ThrMet: 1.27 ± 0.034	1.303ThrAsn: 1.303 ± 0.034	3.304ThrPro: 3.304 ± 0.06	1.677ThrGln: 1.677 ± 0.04	3.643ThrArg: 3.643 ± 0.066	2.789ThrSer: 2.789 ± 0.078	2.92ThrThr: 2.92 ± 0.09	4.314ThrVal: 4.314 ± 0.09	0.694ThrTrp: 0.694 ± 0.024	1.222ThrTyr: 1.222 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	8.418ValAla: 8.418 ± 0.101	0.655ValCys: 0.655 ± 0.023	4.15ValAsp: 4.15 ± 0.069	4.355ValGlu: 4.355 ± 0.069	2.811ValPhe: 2.811 ± 0.055	5.176ValGly: 5.176 ± 0.08	1.408ValHis: 1.408 ± 0.039	4.461ValIle: 4.461 ± 0.078	2.046ValLys: 2.046 ± 0.052	7.351ValLeu: 7.351 ± 0.1	2.183ValMet: 2.183 ± 0.043	1.939ValAsn: 1.939 ± 0.043	3.446ValPro: 3.446 ± 0.058	2.162ValGln: 2.162 ± 0.042	4.368ValArg: 4.368 ± 0.068	4.224ValSer: 4.224 ± 0.084	4.885ValThr: 4.885 ± 0.108	5.431ValVal: 5.431 ± 0.077	0.96ValTrp: 0.96 ± 0.034	1.441ValTyr: 1.441 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.405TrpAla: 1.405 ± 0.038	0.151TrpCys: 0.151 ± 0.012	0.751TrpAsp: 0.751 ± 0.029	0.65TrpGlu: 0.65 ± 0.024	0.542TrpPhe: 0.542 ± 0.021	1.027TrpGly: 1.027 ± 0.033	0.349TrpHis: 0.349 ± 0.017	0.713TrpIle: 0.713 ± 0.027	0.442TrpLys: 0.442 ± 0.021	1.601TrpLeu: 1.601 ± 0.043	0.418TrpMet: 0.418 ± 0.019	0.363TrpAsn: 0.363 ± 0.018	0.737TrpPro: 0.737 ± 0.024	0.58TrpGln: 0.58 ± 0.023	1.148TrpArg: 1.148 ± 0.032	0.827TrpSer: 0.827 ± 0.029	0.728TrpThr: 0.728 ± 0.028	0.903TrpVal: 0.903 ± 0.025	0.218TrpTrp: 0.218 ± 0.014	0.271TrpTyr: 0.271 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.436TyrAla: 2.436 ± 0.045	0.248TyrCys: 0.248 ± 0.014	1.652TyrAsp: 1.652 ± 0.036	1.256TyrGlu: 1.256 ± 0.037	0.86TyrPhe: 0.86 ± 0.026	2.104TyrGly: 2.104 ± 0.042	0.553TyrHis: 0.553 ± 0.02	0.968TyrIle: 0.968 ± 0.03	0.573TyrLys: 0.573 ± 0.027	2.215TyrLeu: 2.215 ± 0.048	0.498TyrMet: 0.498 ± 0.022	0.587TyrAsn: 0.587 ± 0.024	1.045TyrPro: 1.045 ± 0.03	0.676TyrGln: 0.676 ± 0.02	1.622TyrArg: 1.622 ± 0.039	1.111TyrSer: 1.111 ± 0.032	1.089TyrThr: 1.089 ± 0.031	1.511TyrVal: 1.511 ± 0.033	0.347TyrTrp: 0.347 ± 0.019	0.566TyrTyr: 0.566 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4072 proteins (1220550 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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