Mycoplasma parvum str. Indiana
Average proteome isoelectric point is 7.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 580 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5ND49|U5ND49_9MOLU HD_domain domain-containing protein OS=Mycoplasma parvum str. Indiana OX=1403316 GN=PRV_02675 PE=4 SV=1
MM1 pKa = 7.61 SKK3 pKa = 10.85 LSLRR7 pKa = 11.84 TLISIANDD15 pKa = 3.06 VAIEE19 pKa = 4.15 SFGEE23 pKa = 4.15 SSFTFSSLWAKK34 pKa = 8.74 TWTKK38 pKa = 11.1 AKK40 pKa = 10.41 DD41 pKa = 3.74 FKK43 pKa = 11.25 KK44 pKa = 10.81 EE45 pKa = 4.1 NIEE48 pKa = 3.87 DD49 pKa = 4.3 WISSFYY55 pKa = 11.25 LEE57 pKa = 4.31 VLGDD61 pKa = 3.58 PRR63 pKa = 11.84 FVYY66 pKa = 10.24 IGNNMWKK73 pKa = 9.94 LRR75 pKa = 11.84 EE76 pKa = 3.95 FMGLAEE82 pKa = 4.18 YY83 pKa = 10.37 LKK85 pKa = 11.29 VMGKK89 pKa = 9.49 RR90 pKa = 11.84 SKK92 pKa = 11.12 NIVFSEE98 pKa = 4.17 KK99 pKa = 10.83 DD100 pKa = 3.3 LDD102 pKa = 4.77 DD103 pKa = 4.71 EE104 pKa = 5.23 DD105 pKa = 5.04 SSSLEE110 pKa = 3.94 EE111 pKa = 4.03 TTEE114 pKa = 3.95 EE115 pKa = 4.56 LKK117 pKa = 11.18 DD118 pKa = 3.93 EE119 pKa = 4.31 EE120 pKa = 5.94 DD121 pKa = 3.75 EE122 pKa = 6.25 DD123 pKa = 4.99 SDD125 pKa = 5.04 LDD127 pKa = 4.5 SEE129 pKa = 4.78 EE130 pKa = 4.9 EE131 pKa = 4.25 DD132 pKa = 5.15 DD133 pKa = 4.59 SSSSNKK139 pKa = 9.76 EE140 pKa = 3.66 YY141 pKa = 11.11 EE142 pKa = 4.25 EE143 pKa = 4.19 NEE145 pKa = 3.93 EE146 pKa = 4.27 LGQEE150 pKa = 4.13 GSGYY154 pKa = 9.16 QGRR157 pKa = 11.84 IKK159 pKa = 10.91 SNEE162 pKa = 3.95 EE163 pKa = 3.18 EE164 pKa = 4.33 DD165 pKa = 4.03 EE166 pKa = 4.45 EE167 pKa = 5.11 SFEE170 pKa = 4.37
Molecular weight: 19.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.381
IPC2_protein 4.113
IPC_protein 4.05
Toseland 3.884
ProMoST 4.139
Dawson 3.999
Bjellqvist 4.177
Wikipedia 3.859
Rodwell 3.884
Grimsley 3.795
Solomon 3.986
Lehninger 3.948
Nozaki 4.101
DTASelect 4.24
Thurlkill 3.884
EMBOSS 3.872
Sillero 4.151
Patrickios 3.859
IPC_peptide 3.999
IPC2_peptide 4.151
IPC2.peptide.svr19 4.089
Protein with the highest isoelectric point:
>tr|U5NCV4|U5NCV4_9MOLU DUF31 domain-containing protein OS=Mycoplasma parvum str. Indiana OX=1403316 GN=PRV_02160 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.7 QPKK8 pKa = 8.28 KK9 pKa = 8.32 RR10 pKa = 11.84 KK11 pKa = 9.02 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 6.31 GFLKK20 pKa = 10.62 RR21 pKa = 11.84 ISTKK25 pKa = 9.92 SGRR28 pKa = 11.84 NILSARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.47 GRR39 pKa = 11.84 KK40 pKa = 7.94 QLTVSSEE47 pKa = 3.79
Molecular weight: 5.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 10.833
IPC_protein 12.223
Toseland 12.384
ProMoST 12.866
Dawson 12.384
Bjellqvist 12.369
Wikipedia 12.852
Rodwell 12.193
Grimsley 12.427
Solomon 12.881
Lehninger 12.778
Nozaki 12.384
DTASelect 12.369
Thurlkill 12.384
EMBOSS 12.881
Sillero 12.384
Patrickios 11.93
IPC_peptide 12.881
IPC2_peptide 11.857
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
580
0
580
166707
29
2645
287.4
33.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.74 ± 0.069
1.136 ± 0.033
4.206 ± 0.063
8.339 ± 0.116
5.897 ± 0.105
5.246 ± 0.111
1.343 ± 0.033
8.637 ± 0.086
11.246 ± 0.105
10.443 ± 0.113
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.58 ± 0.031
7.005 ± 0.096
2.536 ± 0.044
3.264 ± 0.061
3.458 ± 0.064
8.237 ± 0.092
3.961 ± 0.063
4.417 ± 0.084
1.729 ± 0.05
3.58 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here