Changjiang tombus-like virus 9
Average proteome isoelectric point is 7.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFV5|A0A1L3KFV5_9VIRU Capsid protein OS=Changjiang tombus-like virus 9 OX=1922823 PE=3 SV=1
MM1 pKa = 7.64 SFFEE5 pKa = 4.45 LLRR8 pKa = 11.84 AFLCCATADD17 pKa = 3.75 SGEE20 pKa = 4.08 VKK22 pKa = 10.0 MDD24 pKa = 3.33 VAKK27 pKa = 9.89 TLLRR31 pKa = 11.84 EE32 pKa = 4.33 DD33 pKa = 4.77 CLATDD38 pKa = 4.49 NIEE41 pKa = 4.1 FTHH44 pKa = 6.87 DD45 pKa = 3.63 PKK47 pKa = 10.59 EE48 pKa = 3.95 HH49 pKa = 6.21 HH50 pKa = 5.52 VTRR53 pKa = 11.84 RR54 pKa = 11.84 PAGVRR59 pKa = 11.84 VTDD62 pKa = 3.49 NRR64 pKa = 11.84 EE65 pKa = 3.61 ISPLEE70 pKa = 3.88 PRR72 pKa = 11.84 AGLPVKK78 pKa = 10.18 KK79 pKa = 9.8 CAYY82 pKa = 8.25 RR83 pKa = 11.84 AEE85 pKa = 4.1 MDD87 pKa = 3.57 VLRR90 pKa = 11.84 KK91 pKa = 9.75 LRR93 pKa = 11.84 LAAEE97 pKa = 4.37 DD98 pKa = 3.44 AFGRR102 pKa = 11.84 QQEE105 pKa = 4.41 KK106 pKa = 10.24 KK107 pKa = 10.17 QPDD110 pKa = 4.03 PVTQHH115 pKa = 7.02 RR116 pKa = 11.84 LEE118 pKa = 4.42 CVLNKK123 pKa = 9.46 ATLAAFKK130 pKa = 10.92 VVGATHH136 pKa = 5.86 QCEE139 pKa = 4.32 SWSLLVAIGEE149 pKa = 4.31 LQLDD153 pKa = 3.87 MTHH156 pKa = 6.65 TRR158 pKa = 11.84 SEE160 pKa = 3.85 WKK162 pKa = 10.05 RR163 pKa = 11.84 RR164 pKa = 11.84 LLRR167 pKa = 11.84 LGEE170 pKa = 4.16 LCSIEE175 pKa = 5.15 SGCEE179 pKa = 3.59 WEE181 pKa = 5.15 VEE183 pKa = 4.3 GNPPGLASEE192 pKa = 4.13 PHH194 pKa = 6.37 PPSGEE199 pKa = 4.06 VPVPTDD205 pKa = 2.99 QPTGVHH211 pKa = 6.68 RR212 pKa = 11.84 LCHH215 pKa = 6.11 DD216 pKa = 3.61 VVEE219 pKa = 5.18 VKK221 pKa = 10.19 AHH223 pKa = 4.96 RR224 pKa = 11.84 RR225 pKa = 11.84 IRR227 pKa = 11.84 KK228 pKa = 9.11 DD229 pKa = 3.03 RR230 pKa = 11.84 EE231 pKa = 3.85 GQYY234 pKa = 10.09 VGTVVRR240 pKa = 11.84 EE241 pKa = 3.8 LKK243 pKa = 10.81 NRR245 pKa = 11.84 FGCPKK250 pKa = 8.82 PTEE253 pKa = 4.56 DD254 pKa = 3.55 NLLAVRR260 pKa = 11.84 RR261 pKa = 11.84 SAINIMEE268 pKa = 4.16 KK269 pKa = 10.22 HH270 pKa = 5.65 GVRR273 pKa = 11.84 PSHH276 pKa = 6.77 VGEE279 pKa = 4.2 VVDD282 pKa = 4.98 LVVAGVFVPDD292 pKa = 3.53 RR293 pKa = 11.84 NEE295 pKa = 3.15 IRR297 pKa = 11.84 AANVLASNRR306 pKa = 11.84 ARR308 pKa = 11.84 ALRR311 pKa = 11.84 RR312 pKa = 11.84 EE313 pKa = 4.13 IANAGPSTVYY323 pKa = 10.84 SKK325 pKa = 9.68 MWTKK329 pKa = 10.41 VRR331 pKa = 11.84 DD332 pKa = 3.98 VVWPDD337 pKa = 2.92 GALPGFLEE345 pKa = 4.72 DD346 pKa = 3.83 PGRR349 pKa = 11.84 AXGGLSVVHH358 pKa = 6.76 GISHH362 pKa = 5.81 KK363 pKa = 9.72 TGLRR367 pKa = 11.84 DD368 pKa = 3.24 VRR370 pKa = 11.84 ISVDD374 pKa = 2.71 RR375 pKa = 11.84 HH376 pKa = 4.59 ARR378 pKa = 11.84 AEE380 pKa = 3.83 PRR382 pKa = 11.84 PRR384 pKa = 11.84 RR385 pKa = 11.84 LYY387 pKa = 10.63 SIKK390 pKa = 10.22 EE391 pKa = 3.66 LSANRR396 pKa = 11.84 DD397 pKa = 3.42 LGVNNADD404 pKa = 3.82 INTLEE409 pKa = 4.35 CALLEE414 pKa = 3.92 RR415 pKa = 11.84 MYY417 pKa = 10.3 YY418 pKa = 10.36 CEE420 pKa = 3.99 VGGDD424 pKa = 4.14 FVAPPPVDD432 pKa = 3.22 PGRR435 pKa = 11.84 FTEE438 pKa = 4.24 RR439 pKa = 11.84 LAGFKK444 pKa = 10.27 AALLSNVPEE453 pKa = 4.12 PTVMSCEE460 pKa = 4.13 EE461 pKa = 4.0 VVATYY466 pKa = 10.32 SGRR469 pKa = 11.84 RR470 pKa = 11.84 NTIYY474 pKa = 10.72 RR475 pKa = 11.84 NALNRR480 pKa = 11.84 LTQIGLSRR488 pKa = 11.84 SDD490 pKa = 3.05 AHH492 pKa = 7.28 SIAFVKK498 pKa = 10.19 MEE500 pKa = 4.78 LVDD503 pKa = 3.39 PCKK506 pKa = 10.46 APRR509 pKa = 11.84 CIQPRR514 pKa = 11.84 KK515 pKa = 8.96 PVYY518 pKa = 9.73 NLCLGRR524 pKa = 11.84 FIKK527 pKa = 10.61 SVEE530 pKa = 3.47 HH531 pKa = 6.99 DD532 pKa = 3.3 IYY534 pKa = 11.12 RR535 pKa = 11.84 GIQKK539 pKa = 8.48 TFGDD543 pKa = 4.77 GPTVMKK549 pKa = 10.2 GYY551 pKa = 9.96 NVSDD555 pKa = 3.3 IGRR558 pKa = 11.84 IARR561 pKa = 11.84 GKK563 pKa = 8.84 WRR565 pKa = 11.84 TFKK568 pKa = 11.0 KK569 pKa = 10.13 PVAIGLDD576 pKa = 3.17 AKK578 pKa = 10.7 KK579 pKa = 10.7 FDD581 pKa = 3.49 MHH583 pKa = 7.85 VSEE586 pKa = 5.74 EE587 pKa = 4.04 ALLWEE592 pKa = 4.62 HH593 pKa = 6.7 GVYY596 pKa = 10.63 LDD598 pKa = 5.37 LFRR601 pKa = 11.84 HH602 pKa = 5.07 NPKK605 pKa = 10.33 LKK607 pKa = 10.4 RR608 pKa = 11.84 LLEE611 pKa = 4.03 WQVDD615 pKa = 3.57 NKK617 pKa = 11.1 GAGFCKK623 pKa = 10.25 DD624 pKa = 3.02 GKK626 pKa = 10.23 LKK628 pKa = 10.63 YY629 pKa = 9.42 RR630 pKa = 11.84 VRR632 pKa = 11.84 GRR634 pKa = 11.84 RR635 pKa = 11.84 FSGDD639 pKa = 3.06 MNTGLGNCLLMSAMVYY655 pKa = 10.32 AYY657 pKa = 10.38 AKK659 pKa = 10.55 DD660 pKa = 3.29 RR661 pKa = 11.84 GVGVKK666 pKa = 10.12 LLNNGDD672 pKa = 3.98 DD673 pKa = 3.9 CVVMLEE679 pKa = 4.69 SADD682 pKa = 3.65 LQQFVTGLDD691 pKa = 3.09 EE692 pKa = 4.31 WFLEE696 pKa = 3.93 MGFRR700 pKa = 11.84 MVAEE704 pKa = 4.04 EE705 pKa = 4.21 PVYY708 pKa = 10.55 KK709 pKa = 10.63 LHH711 pKa = 7.25 QIEE714 pKa = 4.81 FCQMHH719 pKa = 7.29 PIEE722 pKa = 6.36 IGDD725 pKa = 3.79 EE726 pKa = 3.92 CRR728 pKa = 11.84 MVRR731 pKa = 11.84 NIPSTLRR738 pKa = 11.84 KK739 pKa = 8.88 DD740 pKa = 3.58 TLTVHH745 pKa = 6.91 PLSNQKK751 pKa = 9.46 HH752 pKa = 4.82 RR753 pKa = 11.84 EE754 pKa = 3.94 KK755 pKa = 10.18 WCTAVGMGGLALTGGVPIMQDD776 pKa = 3.7 FYY778 pKa = 11.91 QMFQRR783 pKa = 11.84 IGCNRR788 pKa = 11.84 SSNIANDD795 pKa = 3.58 PTFATGLRR803 pKa = 11.84 LMSKK807 pKa = 10.31 GMAEE811 pKa = 4.89 HH812 pKa = 6.39 YY813 pKa = 10.05 RR814 pKa = 11.84 EE815 pKa = 4.37 PDD817 pKa = 2.41 PWTRR821 pKa = 11.84 VQVFEE826 pKa = 4.35 AWGITPDD833 pKa = 3.38 EE834 pKa = 4.08 QKK836 pKa = 10.93 AIEE839 pKa = 4.31 RR840 pKa = 11.84 YY841 pKa = 9.55 YY842 pKa = 11.15 RR843 pKa = 11.84 DD844 pKa = 3.76 FSLSDD849 pKa = 3.3 QPVSGEE855 pKa = 3.87 QANVRR860 pKa = 11.84 CHH862 pKa = 7.38 LLLQLL867 pKa = 4.53
Molecular weight: 97.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.483
IPC2_protein 7.834
IPC_protein 7.6
Toseland 7.234
ProMoST 8.244
Dawson 8.361
Bjellqvist 8.785
Wikipedia 8.273
Rodwell 8.39
Grimsley 7.293
Solomon 8.39
Lehninger 8.419
Nozaki 9.063
DTASelect 8.478
Thurlkill 8.536
EMBOSS 8.565
Sillero 8.887
Patrickios 4.622
IPC_peptide 8.39
IPC2_peptide 7.775
IPC2.peptide.svr19 7.862
Protein with the highest isoelectric point:
>tr|A0A1L3KFV5|A0A1L3KFV5_9VIRU Capsid protein OS=Changjiang tombus-like virus 9 OX=1922823 PE=3 SV=1
MM1 pKa = 7.44 PPVVAAVKK9 pKa = 10.39 AGEE12 pKa = 4.31 LRR14 pKa = 11.84 QQKK17 pKa = 10.74 YY18 pKa = 10.88 NMVKK22 pKa = 10.35 KK23 pKa = 10.56 ANKK26 pKa = 9.43 KK27 pKa = 7.4 MKK29 pKa = 9.21 KK30 pKa = 7.63 TIKK33 pKa = 9.28 VAKK36 pKa = 8.93 KK37 pKa = 9.46 KK38 pKa = 11.05 SEE40 pKa = 3.65 ITEE43 pKa = 4.06 LGRR46 pKa = 11.84 VLRR49 pKa = 11.84 TLGGIGGGFAGGMIGQGAAGSAVGTGLGAAVSRR82 pKa = 11.84 WLGQGDD88 pKa = 4.02 YY89 pKa = 10.89 SVTSNSVLRR98 pKa = 11.84 AATTVPSMHH107 pKa = 7.02 KK108 pKa = 10.03 NDD110 pKa = 3.34 QTVVVRR116 pKa = 11.84 HH117 pKa = 5.82 KK118 pKa = 10.87 EE119 pKa = 3.87 FVGEE123 pKa = 4.23 LRR125 pKa = 11.84 GNQNFTVAYY134 pKa = 9.41 RR135 pKa = 11.84 YY136 pKa = 10.34 SLNPGIALTFPWLHH150 pKa = 6.71 SIAAQYY156 pKa = 10.38 SEE158 pKa = 3.69 YY159 pKa = 10.21 RR160 pKa = 11.84 IRR162 pKa = 11.84 GMVFHH167 pKa = 6.44 YY168 pKa = 10.68 VPTSGASVASSNTALGSVMFQTSYY192 pKa = 10.67 RR193 pKa = 11.84 ASEE196 pKa = 4.15 NPPGSKK202 pKa = 8.86 IEE204 pKa = 3.93 MMNEE208 pKa = 3.32 YY209 pKa = 8.79 WASEE213 pKa = 3.76 GRR215 pKa = 11.84 PCDD218 pKa = 3.97 EE219 pKa = 4.11 FCHH222 pKa = 6.84 PIEE225 pKa = 5.36 CDD227 pKa = 3.21 PKK229 pKa = 9.53 EE230 pKa = 4.09 NPFNVQYY237 pKa = 11.03 VRR239 pKa = 11.84 TGGLPAGEE247 pKa = 4.2 NLLMYY252 pKa = 10.55 DD253 pKa = 4.45 LGTTTVAVTGMQTTGTVLGDD273 pKa = 3.17 IWCTYY278 pKa = 7.91 EE279 pKa = 5.08 VEE281 pKa = 4.24 LKK283 pKa = 10.57 KK284 pKa = 10.68 PKK286 pKa = 9.33 LTGLNTEE293 pKa = 4.45 ATRR296 pKa = 11.84 SLYY299 pKa = 11.05 SNSSTGITNANPLGSGPVLSTIDD322 pKa = 3.4 GVSAVGSQITFPGNLDD338 pKa = 3.19 GKK340 pKa = 9.27 YY341 pKa = 10.07 VVVLTISGFTAAGGVTVMTSGPATLTSLAQAQGSASLSYY380 pKa = 10.7 VAILEE385 pKa = 4.31 FTGSVNTTLLTISLTTLTGATLSTLRR411 pKa = 11.84 ITEE414 pKa = 4.06 WNADD418 pKa = 3.66 FAA420 pKa = 6.14
Molecular weight: 44.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.469
IPC2_protein 8.434
IPC_protein 8.361
Toseland 8.814
ProMoST 8.785
Dawson 9.18
Bjellqvist 9.092
Wikipedia 9.355
Rodwell 9.311
Grimsley 9.194
Solomon 9.268
Lehninger 9.224
Nozaki 9.136
DTASelect 8.99
Thurlkill 9.063
EMBOSS 9.311
Sillero 9.238
Patrickios 4.355
IPC_peptide 9.253
IPC2_peptide 7.805
IPC2.peptide.svr19 7.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1287
420
867
643.5
70.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.77 ± 0.554
2.176 ± 0.652
4.507 ± 1.261
6.138 ± 0.861
3.186 ± 0.175
8.702 ± 1.327
2.409 ± 0.65
3.807 ± 0.128
5.128 ± 0.195
8.936 ± 0.321
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.797 ± 0.032
4.118 ± 0.343
5.128 ± 0.449
2.953 ± 0.076
7.148 ± 1.908
5.905 ± 1.422
6.449 ± 2.275
8.625 ± 0.028
1.321 ± 0.07
2.72 ± 0.454
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here