Arumowot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Arumowot phlebovirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1SV58|I1SV58_9VIRU Nucleoprotein OS=Arumowot virus OX=904698 PE=3 SV=1
MM1 pKa = 7.83ASPYY5 pKa = 10.96NYY7 pKa = 9.59IHH9 pKa = 7.72DD10 pKa = 4.53LVTPNYY16 pKa = 9.42NGPIPGEE23 pKa = 4.13VVVSFEE29 pKa = 4.28PVNSYY34 pKa = 10.71VNNPVCVYY42 pKa = 10.49EE43 pKa = 4.43DD44 pKa = 4.07LEE46 pKa = 4.44FEE48 pKa = 4.05ISGMGLCTMTSEE60 pKa = 4.54SLYY63 pKa = 11.24DD64 pKa = 3.93FLDD67 pKa = 3.88SGLMPWRR74 pKa = 11.84WGKK77 pKa = 11.12GMFEE81 pKa = 4.23SRR83 pKa = 11.84LTAMEE88 pKa = 4.36TPLFDD93 pKa = 5.17SFLKK97 pKa = 9.52QIPMIPTTQLFSVGRR112 pKa = 11.84DD113 pKa = 3.51PLRR116 pKa = 11.84AALSWPTGRR125 pKa = 11.84CSLRR129 pKa = 11.84FFRR132 pKa = 11.84QQWGSDD138 pKa = 2.87AMSYY142 pKa = 10.26QSNRR146 pKa = 11.84KK147 pKa = 8.78LRR149 pKa = 11.84ARR151 pKa = 11.84LLFQSTQSSDD161 pKa = 3.41LEE163 pKa = 4.23TAIITLAKK171 pKa = 9.83DD172 pKa = 3.11IRR174 pKa = 11.84GMSSILDD181 pKa = 3.65FPLNLIPGFDD191 pKa = 3.84LLKK194 pKa = 10.56CVSILQFIRR203 pKa = 11.84ALRR206 pKa = 11.84SYY208 pKa = 10.72PEE210 pKa = 4.12TEE212 pKa = 5.04AEE214 pKa = 4.07RR215 pKa = 11.84QSNGLRR221 pKa = 11.84DD222 pKa = 4.02DD223 pKa = 4.74LDD225 pKa = 3.72IFLGSARR232 pKa = 11.84EE233 pKa = 3.91ALSRR237 pKa = 11.84DD238 pKa = 3.71FPALIPAITPEE249 pKa = 4.39SDD251 pKa = 3.27DD252 pKa = 4.24SLWDD256 pKa = 3.63SGSSCEE262 pKa = 5.43DD263 pKa = 3.49DD264 pKa = 3.73VDD266 pKa = 3.79VV267 pKa = 5.14

Molecular weight: 30.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1SV58|I1SV58_9VIRU Nucleoprotein OS=Arumowot virus OX=904698 PE=3 SV=1
MM1 pKa = 8.0DD2 pKa = 5.09NYY4 pKa = 11.43AKK6 pKa = 10.33IAIDD10 pKa = 3.69FAGDD14 pKa = 3.87AIDD17 pKa = 3.96ASEE20 pKa = 4.94LIALAEE26 pKa = 4.01EE27 pKa = 4.93FSFNGFDD34 pKa = 3.8PNAIMKK40 pKa = 9.1LIIEE44 pKa = 4.37RR45 pKa = 11.84GGNNWKK51 pKa = 10.14EE52 pKa = 4.11DD53 pKa = 3.77AKK55 pKa = 10.67MMIVIGITRR64 pKa = 11.84GNKK67 pKa = 7.77IKK69 pKa = 11.12KK70 pKa = 9.03MMDD73 pKa = 2.75RR74 pKa = 11.84MGEE77 pKa = 3.91EE78 pKa = 4.5GKK80 pKa = 8.68TKK82 pKa = 10.67LKK84 pKa = 10.9YY85 pKa = 10.61LVDD88 pKa = 3.65KK89 pKa = 11.09YY90 pKa = 10.9KK91 pKa = 10.93LKK93 pKa = 10.34EE94 pKa = 4.0ASPGARR100 pKa = 11.84DD101 pKa = 3.5LTLSRR106 pKa = 11.84VAAVLAPWTVQALRR120 pKa = 11.84VLEE123 pKa = 4.26NKK125 pKa = 10.51LPVTGATMDD134 pKa = 4.16SLSHH138 pKa = 6.41NYY140 pKa = 8.57PRR142 pKa = 11.84ALMHH146 pKa = 6.33SAAGGILDD154 pKa = 4.78PGLGDD159 pKa = 3.54ACFGALLQAHH169 pKa = 6.41SLYY172 pKa = 10.85LDD174 pKa = 3.43TFSRR178 pKa = 11.84LINPPLRR185 pKa = 11.84SKK187 pKa = 10.8SKK189 pKa = 10.89QEE191 pKa = 3.78VAQSFSSALQAAYY204 pKa = 10.36SSNFFSPAQKK214 pKa = 10.31RR215 pKa = 11.84QILQTMGLIDD225 pKa = 4.78MNLQPTKK232 pKa = 10.8AVLDD236 pKa = 3.58AAGIYY241 pKa = 10.95NSMM244 pKa = 4.28

Molecular weight: 26.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3814

244

2093

953.5

107.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.929 ± 1.267

2.727 ± 0.878

5.716 ± 0.805

6.266 ± 0.644

4.274 ± 0.413

6.162 ± 0.498

1.966 ± 0.314

6.66 ± 0.224

6.607 ± 0.713

9.439 ± 0.666

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.609

4.143 ± 0.169

4.064 ± 0.401

3.54 ± 0.234

4.929 ± 0.743

9.622 ± 0.516

5.821 ± 0.564

6.135 ± 0.624

1.337 ± 0.119

3.12 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski