Lactobacillus phage LJ

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Junavirus; Lactobacillus virus LJ

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2D1GPL6|A0A2D1GPL6_9CAUD Head-to-tail joining protein OS=Lactobacillus phage LJ OX=2041454 GN=LJ_45 PE=4 SV=1
MM1 pKa = 7.62SGIEE5 pKa = 3.82RR6 pKa = 11.84STNYY10 pKa = 10.77DD11 pKa = 3.21MEE13 pKa = 5.41RR14 pKa = 11.84YY15 pKa = 9.2QYY17 pKa = 11.14LSEE20 pKa = 4.6YY21 pKa = 9.66DD22 pKa = 3.22TALPDD27 pKa = 4.57DD28 pKa = 5.23LPTIPLAVSEE38 pKa = 4.88EE39 pKa = 4.22IKK41 pKa = 10.77GSYY44 pKa = 10.4GSLSLRR50 pKa = 11.84DD51 pKa = 4.03EE52 pKa = 5.24LDD54 pKa = 3.72TADD57 pKa = 4.33STSDD61 pKa = 3.49DD62 pKa = 4.23PSVIDD67 pKa = 3.91YY68 pKa = 10.65IEE70 pKa = 4.3NSEE73 pKa = 4.69SVFAEE78 pKa = 4.67AWCRR82 pKa = 11.84GMWIVKK88 pKa = 7.59EE89 pKa = 4.21TGEE92 pKa = 4.0VTGYY96 pKa = 10.91DD97 pKa = 3.61EE98 pKa = 4.36QQ99 pKa = 5.35

Molecular weight:
11.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2D1GPJ8|A0A2D1GPJ8_9CAUD Uncharacterized protein OS=Lactobacillus phage LJ OX=2041454 GN=LJ_24 PE=4 SV=1
MM1 pKa = 7.35IKK3 pKa = 10.17LWQGVAMRR11 pKa = 11.84TDD13 pKa = 4.2CDD15 pKa = 3.81SQAGSIPACHH25 pKa = 6.28IVQFSDD31 pKa = 3.15RR32 pKa = 11.84TQLAMTPSDD41 pKa = 3.35NGRR44 pKa = 11.84ASSARR49 pKa = 11.84RR50 pKa = 11.84IKK52 pKa = 10.31FVALLGQKK60 pKa = 9.06QEE62 pKa = 3.95QPKK65 pKa = 10.49AKK67 pKa = 8.98WSPWTTTQAHH77 pKa = 5.19GKK79 pKa = 8.2VYY81 pKa = 10.16RR82 pKa = 11.84VRR84 pKa = 11.84IPTVALKK91 pKa = 10.66HH92 pKa = 4.8FTFCEE97 pKa = 4.02VLFLYY102 pKa = 10.14IISEE106 pKa = 4.01ARR108 pKa = 11.84TT109 pKa = 3.38

Molecular weight:
12.32 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13760

36

1620

211.7

23.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.488 ± 0.608

0.552 ± 0.133

7.573 ± 0.367

5.749 ± 0.344

3.423 ± 0.164

6.679 ± 0.345

1.672 ± 0.191

6.105 ± 0.222

7.384 ± 0.346

7.551 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.152

5.182 ± 0.307

3.496 ± 0.19

4.295 ± 0.174

4.484 ± 0.294

6.73 ± 0.307

6.693 ± 0.292

6.185 ± 0.234

1.424 ± 0.124

3.692 ± 0.324

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski