Lactobacillus phage LJ
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D1GPL6|A0A2D1GPL6_9CAUD Head-to-tail joining protein OS=Lactobacillus phage LJ OX=2041454 GN=LJ_45 PE=4 SV=1
MM1 pKa = 7.62 SGIEE5 pKa = 3.82 RR6 pKa = 11.84 STNYY10 pKa = 10.77 DD11 pKa = 3.21 MEE13 pKa = 5.41 RR14 pKa = 11.84 YY15 pKa = 9.2 QYY17 pKa = 11.14 LSEE20 pKa = 4.6 YY21 pKa = 9.66 DD22 pKa = 3.22 TALPDD27 pKa = 4.57 DD28 pKa = 5.23 LPTIPLAVSEE38 pKa = 4.88 EE39 pKa = 4.22 IKK41 pKa = 10.77 GSYY44 pKa = 10.4 GSLSLRR50 pKa = 11.84 DD51 pKa = 4.03 EE52 pKa = 5.24 LDD54 pKa = 3.72 TADD57 pKa = 4.33 STSDD61 pKa = 3.49 DD62 pKa = 4.23 PSVIDD67 pKa = 3.91 YY68 pKa = 10.65 IEE70 pKa = 4.3 NSEE73 pKa = 4.69 SVFAEE78 pKa = 4.67 AWCRR82 pKa = 11.84 GMWIVKK88 pKa = 7.59 EE89 pKa = 4.21 TGEE92 pKa = 4.0 VTGYY96 pKa = 10.91 DD97 pKa = 3.61 EE98 pKa = 4.36 QQ99 pKa = 5.35
Molecular weight: 11.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.732
IPC_protein 3.681
Toseland 3.478
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.579
Rodwell 3.516
Grimsley 3.389
Solomon 3.643
Lehninger 3.592
Nozaki 3.783
DTASelect 3.961
Thurlkill 3.528
EMBOSS 3.592
Sillero 3.795
Patrickios 0.846
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.729
Protein with the highest isoelectric point:
>tr|A0A2D1GPJ8|A0A2D1GPJ8_9CAUD Uncharacterized protein OS=Lactobacillus phage LJ OX=2041454 GN=LJ_24 PE=4 SV=1
MM1 pKa = 7.35 IKK3 pKa = 10.17 LWQGVAMRR11 pKa = 11.84 TDD13 pKa = 4.2 CDD15 pKa = 3.81 SQAGSIPACHH25 pKa = 6.28 IVQFSDD31 pKa = 3.15 RR32 pKa = 11.84 TQLAMTPSDD41 pKa = 3.35 NGRR44 pKa = 11.84 ASSARR49 pKa = 11.84 RR50 pKa = 11.84 IKK52 pKa = 10.31 FVALLGQKK60 pKa = 9.06 QEE62 pKa = 3.95 QPKK65 pKa = 10.49 AKK67 pKa = 8.98 WSPWTTTQAHH77 pKa = 5.19 GKK79 pKa = 8.2 VYY81 pKa = 10.16 RR82 pKa = 11.84 VRR84 pKa = 11.84 IPTVALKK91 pKa = 10.66 HH92 pKa = 4.8 FTFCEE97 pKa = 4.02 VLFLYY102 pKa = 10.14 IISEE106 pKa = 4.01 ARR108 pKa = 11.84 TT109 pKa = 3.38
Molecular weight: 12.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.174
IPC2_protein 9.224
IPC_protein 9.282
Toseland 10.16
ProMoST 9.736
Dawson 10.292
Bjellqvist 9.955
Wikipedia 10.423
Rodwell 10.774
Grimsley 10.335
Solomon 10.335
Lehninger 10.321
Nozaki 10.218
DTASelect 9.911
Thurlkill 10.175
EMBOSS 10.54
Sillero 10.218
Patrickios 10.57
IPC_peptide 10.35
IPC2_peptide 8.858
IPC2.peptide.svr19 8.152
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
13760
36
1620
211.7
23.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.488 ± 0.608
0.552 ± 0.133
7.573 ± 0.367
5.749 ± 0.344
3.423 ± 0.164
6.679 ± 0.345
1.672 ± 0.191
6.105 ± 0.222
7.384 ± 0.346
7.551 ± 0.243
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.645 ± 0.152
5.182 ± 0.307
3.496 ± 0.19
4.295 ± 0.174
4.484 ± 0.294
6.73 ± 0.307
6.693 ± 0.292
6.185 ± 0.234
1.424 ± 0.124
3.692 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here