Amino acid dipepetide frequency for Lactobacillus phage LJ

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.794AlaAla: 8.794 ± 2.427
0.363AlaCys: 0.363 ± 0.171
6.759AlaAsp: 6.759 ± 0.937
5.16AlaGlu: 5.16 ± 0.496
2.762AlaPhe: 2.762 ± 0.482
5.16AlaGly: 5.16 ± 0.786
1.163AlaHis: 1.163 ± 0.284
5.596AlaIle: 5.596 ± 0.753
6.032AlaLys: 6.032 ± 0.967
6.396AlaLeu: 6.396 ± 0.697
2.98AlaMet: 2.98 ± 0.468
4.215AlaAsn: 4.215 ± 0.477
1.962AlaPro: 1.962 ± 0.345
3.561AlaGln: 3.561 ± 0.568
2.689AlaArg: 2.689 ± 0.383
5.088AlaSer: 5.088 ± 0.985
5.451AlaThr: 5.451 ± 0.577
6.541AlaVal: 6.541 ± 0.643
1.09AlaTrp: 1.09 ± 0.282
3.125AlaTyr: 3.125 ± 0.529
0.0AlaXaa: 0.0 ± 0.0
Cys
0.291CysAla: 0.291 ± 0.143
0.218CysCys: 0.218 ± 0.141
0.291CysAsp: 0.291 ± 0.154
0.436CysGlu: 0.436 ± 0.181
0.145CysPhe: 0.145 ± 0.115
0.509CysGly: 0.509 ± 0.176
0.145CysHis: 0.145 ± 0.096
0.363CysIle: 0.363 ± 0.201
0.363CysLys: 0.363 ± 0.153
0.363CysLeu: 0.363 ± 0.206
0.073CysMet: 0.073 ± 0.084
0.291CysAsn: 0.291 ± 0.163
0.218CysPro: 0.218 ± 0.11
0.218CysGln: 0.218 ± 0.118
0.363CysArg: 0.363 ± 0.191
0.218CysSer: 0.218 ± 0.124
0.291CysThr: 0.291 ± 0.145
0.291CysVal: 0.291 ± 0.153
0.145CysTrp: 0.145 ± 0.093
0.291CysTyr: 0.291 ± 0.161
0.0CysXaa: 0.0 ± 0.0
Asp
5.015AspAla: 5.015 ± 0.635
0.291AspCys: 0.291 ± 0.123
5.887AspAsp: 5.887 ± 0.907
4.724AspGlu: 4.724 ± 0.831
3.053AspPhe: 3.053 ± 0.434
7.123AspGly: 7.123 ± 1.247
1.599AspHis: 1.599 ± 0.388
3.852AspIle: 3.852 ± 0.576
4.797AspLys: 4.797 ± 0.601
5.16AspLeu: 5.16 ± 0.616
2.108AspMet: 2.108 ± 0.433
3.125AspAsn: 3.125 ± 0.605
3.925AspPro: 3.925 ± 0.576
3.053AspGln: 3.053 ± 0.518
3.852AspArg: 3.852 ± 0.633
4.361AspSer: 4.361 ± 0.536
4.724AspThr: 4.724 ± 0.569
3.925AspVal: 3.925 ± 0.615
1.526AspTrp: 1.526 ± 0.303
3.634AspTyr: 3.634 ± 0.604
0.0AspXaa: 0.0 ± 0.0
Glu
4.652GluAla: 4.652 ± 0.737
0.436GluCys: 0.436 ± 0.179
3.416GluAsp: 3.416 ± 0.445
3.053GluGlu: 3.053 ± 0.564
1.599GluPhe: 1.599 ± 0.389
2.689GluGly: 2.689 ± 0.437
1.09GluHis: 1.09 ± 0.269
3.271GluIle: 3.271 ± 0.468
4.724GluLys: 4.724 ± 0.776
4.942GluLeu: 4.942 ± 0.623
1.672GluMet: 1.672 ± 0.301
2.689GluAsn: 2.689 ± 0.483
1.962GluPro: 1.962 ± 0.451
2.98GluGln: 2.98 ± 0.4
3.489GluArg: 3.489 ± 0.594
2.98GluSer: 2.98 ± 0.505
3.779GluThr: 3.779 ± 0.546
4.07GluVal: 4.07 ± 0.598
1.454GluTrp: 1.454 ± 0.298
2.544GluTyr: 2.544 ± 0.486
0.0GluXaa: 0.0 ± 0.0
Phe
2.471PheAla: 2.471 ± 0.425
0.291PheCys: 0.291 ± 0.16
2.689PheAsp: 2.689 ± 0.404
1.817PheGlu: 1.817 ± 0.461
0.727PhePhe: 0.727 ± 0.25
2.762PheGly: 2.762 ± 0.453
0.509PheHis: 0.509 ± 0.2
2.18PheIle: 2.18 ± 0.608
2.326PheLys: 2.326 ± 0.344
2.98PheLeu: 2.98 ± 0.636
1.018PheMet: 1.018 ± 0.257
2.398PheAsn: 2.398 ± 0.482
0.654PhePro: 0.654 ± 0.207
1.454PheGln: 1.454 ± 0.339
1.381PheArg: 1.381 ± 0.332
2.253PheSer: 2.253 ± 0.453
2.689PheThr: 2.689 ± 0.56
1.962PheVal: 1.962 ± 0.372
0.581PheTrp: 0.581 ± 0.28
1.09PheTyr: 1.09 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
4.652GlyAla: 4.652 ± 0.879
0.145GlyCys: 0.145 ± 0.122
4.579GlyAsp: 4.579 ± 0.615
3.634GlyGlu: 3.634 ± 0.487
2.616GlyPhe: 2.616 ± 0.434
3.997GlyGly: 3.997 ± 0.475
1.308GlyHis: 1.308 ± 0.357
4.942GlyIle: 4.942 ± 0.653
5.378GlyLys: 5.378 ± 0.607
3.779GlyLeu: 3.779 ± 0.611
2.253GlyMet: 2.253 ± 0.279
2.907GlyAsn: 2.907 ± 0.593
1.744GlyPro: 1.744 ± 0.428
2.253GlyGln: 2.253 ± 0.484
3.271GlyArg: 3.271 ± 0.534
4.724GlySer: 4.724 ± 0.522
5.378GlyThr: 5.378 ± 0.675
5.015GlyVal: 5.015 ± 0.71
1.308GlyTrp: 1.308 ± 0.328
2.907GlyTyr: 2.907 ± 0.515
0.0GlyXaa: 0.0 ± 0.0
His
1.526HisAla: 1.526 ± 0.313
0.073HisCys: 0.073 ± 0.07
1.454HisAsp: 1.454 ± 0.324
0.436HisGlu: 0.436 ± 0.189
0.727HisPhe: 0.727 ± 0.267
1.236HisGly: 1.236 ± 0.31
0.436HisHis: 0.436 ± 0.179
1.454HisIle: 1.454 ± 0.374
0.727HisLys: 0.727 ± 0.264
1.526HisLeu: 1.526 ± 0.313
0.218HisMet: 0.218 ± 0.141
0.291HisAsn: 0.291 ± 0.136
0.436HisPro: 0.436 ± 0.161
0.799HisGln: 0.799 ± 0.243
0.581HisArg: 0.581 ± 0.256
1.454HisSer: 1.454 ± 0.426
1.09HisThr: 1.09 ± 0.245
1.018HisVal: 1.018 ± 0.23
0.363HisTrp: 0.363 ± 0.161
0.872HisTyr: 0.872 ± 0.307
0.0HisXaa: 0.0 ± 0.0
Ile
4.942IleAla: 4.942 ± 0.481
0.363IleCys: 0.363 ± 0.151
5.233IleAsp: 5.233 ± 0.66
4.87IleGlu: 4.87 ± 0.616
1.672IlePhe: 1.672 ± 0.357
3.852IleGly: 3.852 ± 0.451
1.09IleHis: 1.09 ± 0.267
3.198IleIle: 3.198 ± 0.461
5.451IleLys: 5.451 ± 0.591
3.125IleLeu: 3.125 ± 0.472
1.526IleMet: 1.526 ± 0.325
3.707IleAsn: 3.707 ± 0.527
2.616IlePro: 2.616 ± 0.405
1.89IleGln: 1.89 ± 0.349
2.616IleArg: 2.616 ± 0.583
4.433IleSer: 4.433 ± 0.647
4.288IleThr: 4.288 ± 0.641
3.779IleVal: 3.779 ± 0.411
0.727IleTrp: 0.727 ± 0.198
1.672IleTyr: 1.672 ± 0.39
0.0IleXaa: 0.0 ± 0.0
Lys
6.977LysAla: 6.977 ± 0.727
0.436LysCys: 0.436 ± 0.187
4.506LysAsp: 4.506 ± 0.442
3.925LysGlu: 3.925 ± 0.643
2.471LysPhe: 2.471 ± 0.41
4.797LysGly: 4.797 ± 0.803
0.799LysHis: 0.799 ± 0.273
3.852LysIle: 3.852 ± 0.573
4.942LysLys: 4.942 ± 0.705
6.178LysLeu: 6.178 ± 0.75
2.18LysMet: 2.18 ± 0.375
3.997LysAsn: 3.997 ± 0.614
2.762LysPro: 2.762 ± 0.546
4.143LysGln: 4.143 ± 0.595
3.707LysArg: 3.707 ± 0.581
5.16LysSer: 5.16 ± 0.663
4.724LysThr: 4.724 ± 0.642
5.088LysVal: 5.088 ± 0.568
0.727LysTrp: 0.727 ± 0.203
2.471LysTyr: 2.471 ± 0.425
0.0LysXaa: 0.0 ± 0.0
Leu
6.396LeuAla: 6.396 ± 0.658
0.581LeuCys: 0.581 ± 0.231
5.742LeuAsp: 5.742 ± 0.779
4.361LeuGlu: 4.361 ± 0.501
2.471LeuPhe: 2.471 ± 0.311
5.088LeuGly: 5.088 ± 0.766
1.381LeuHis: 1.381 ± 0.317
5.306LeuIle: 5.306 ± 0.722
6.468LeuLys: 6.468 ± 0.928
5.16LeuLeu: 5.16 ± 0.572
1.89LeuMet: 1.89 ± 0.331
3.271LeuAsn: 3.271 ± 0.448
3.634LeuPro: 3.634 ± 0.429
2.762LeuGln: 2.762 ± 0.439
3.707LeuArg: 3.707 ± 0.522
5.814LeuSer: 5.814 ± 0.684
5.233LeuThr: 5.233 ± 0.519
3.125LeuVal: 3.125 ± 0.408
0.654LeuTrp: 0.654 ± 0.212
2.471LeuTyr: 2.471 ± 0.497
0.0LeuXaa: 0.0 ± 0.0
Met
2.326MetAla: 2.326 ± 0.436
0.0MetCys: 0.0 ± 0.0
1.526MetAsp: 1.526 ± 0.276
0.945MetGlu: 0.945 ± 0.232
1.163MetPhe: 1.163 ± 0.296
1.381MetGly: 1.381 ± 0.288
0.291MetHis: 0.291 ± 0.131
1.744MetIle: 1.744 ± 0.338
1.89MetLys: 1.89 ± 0.321
1.526MetLeu: 1.526 ± 0.316
0.581MetMet: 0.581 ± 0.265
2.253MetAsn: 2.253 ± 0.377
1.236MetPro: 1.236 ± 0.333
1.454MetGln: 1.454 ± 0.356
1.599MetArg: 1.599 ± 0.353
1.744MetSer: 1.744 ± 0.363
2.471MetThr: 2.471 ± 0.431
1.236MetVal: 1.236 ± 0.269
0.218MetTrp: 0.218 ± 0.112
0.872MetTyr: 0.872 ± 0.212
0.0MetXaa: 0.0 ± 0.0
Asn
4.724AsnAla: 4.724 ± 0.657
0.291AsnCys: 0.291 ± 0.177
4.652AsnAsp: 4.652 ± 0.587
3.198AsnGlu: 3.198 ± 0.487
1.454AsnPhe: 1.454 ± 0.374
4.797AsnGly: 4.797 ± 0.573
1.236AsnHis: 1.236 ± 0.256
2.689AsnIle: 2.689 ± 0.419
4.07AsnLys: 4.07 ± 0.486
2.98AsnLeu: 2.98 ± 0.444
1.599AsnMet: 1.599 ± 0.278
2.616AsnAsn: 2.616 ± 0.427
1.817AsnPro: 1.817 ± 0.288
1.744AsnGln: 1.744 ± 0.356
2.544AsnArg: 2.544 ± 0.455
3.634AsnSer: 3.634 ± 0.638
3.125AsnThr: 3.125 ± 0.518
2.326AsnVal: 2.326 ± 0.402
0.581AsnTrp: 0.581 ± 0.214
1.744AsnTyr: 1.744 ± 0.391
0.0AsnXaa: 0.0 ± 0.0
Pro
3.416ProAla: 3.416 ± 0.418
0.145ProCys: 0.145 ± 0.114
3.125ProAsp: 3.125 ± 0.577
2.616ProGlu: 2.616 ± 0.402
1.163ProPhe: 1.163 ± 0.261
1.163ProGly: 1.163 ± 0.392
0.727ProHis: 0.727 ± 0.247
1.962ProIle: 1.962 ± 0.439
3.707ProLys: 3.707 ± 0.579
2.689ProLeu: 2.689 ± 0.492
0.654ProMet: 0.654 ± 0.287
1.89ProAsn: 1.89 ± 0.401
0.509ProPro: 0.509 ± 0.271
1.236ProGln: 1.236 ± 0.301
0.654ProArg: 0.654 ± 0.209
3.198ProSer: 3.198 ± 0.415
1.962ProThr: 1.962 ± 0.357
2.398ProVal: 2.398 ± 0.395
0.363ProTrp: 0.363 ± 0.156
1.381ProTyr: 1.381 ± 0.339
0.0ProXaa: 0.0 ± 0.0
Gln
4.87GlnAla: 4.87 ± 0.554
0.073GlnCys: 0.073 ± 0.074
2.253GlnAsp: 2.253 ± 0.394
2.253GlnGlu: 2.253 ± 0.414
1.744GlnPhe: 1.744 ± 0.361
2.544GlnGly: 2.544 ± 0.426
0.872GlnHis: 0.872 ± 0.273
2.471GlnIle: 2.471 ± 0.362
3.053GlnLys: 3.053 ± 0.454
4.652GlnLeu: 4.652 ± 0.634
1.018GlnMet: 1.018 ± 0.282
2.035GlnAsn: 2.035 ± 0.386
1.236GlnPro: 1.236 ± 0.338
2.398GlnGln: 2.398 ± 0.604
1.962GlnArg: 1.962 ± 0.34
2.035GlnSer: 2.035 ± 0.36
2.398GlnThr: 2.398 ± 0.419
2.253GlnVal: 2.253 ± 0.46
1.09GlnTrp: 1.09 ± 0.231
1.744GlnTyr: 1.744 ± 0.31
0.0GlnXaa: 0.0 ± 0.0
Arg
3.053ArgAla: 3.053 ± 0.484
0.291ArgCys: 0.291 ± 0.189
3.343ArgAsp: 3.343 ± 0.636
2.762ArgGlu: 2.762 ± 0.504
1.744ArgPhe: 1.744 ± 0.347
2.98ArgGly: 2.98 ± 0.388
0.945ArgHis: 0.945 ± 0.297
2.98ArgIle: 2.98 ± 0.597
3.198ArgLys: 3.198 ± 0.515
3.707ArgLeu: 3.707 ± 0.639
1.308ArgMet: 1.308 ± 0.294
2.18ArgAsn: 2.18 ± 0.383
0.727ArgPro: 0.727 ± 0.264
2.616ArgGln: 2.616 ± 0.532
1.599ArgArg: 1.599 ± 0.318
2.762ArgSer: 2.762 ± 0.513
3.125ArgThr: 3.125 ± 0.464
3.271ArgVal: 3.271 ± 0.478
0.654ArgTrp: 0.654 ± 0.184
1.599ArgTyr: 1.599 ± 0.426
0.0ArgXaa: 0.0 ± 0.0
Ser
5.015SerAla: 5.015 ± 0.693
0.145SerCys: 0.145 ± 0.114
5.306SerAsp: 5.306 ± 0.61
3.489SerGlu: 3.489 ± 0.578
2.326SerPhe: 2.326 ± 0.356
6.032SerGly: 6.032 ± 0.786
0.727SerHis: 0.727 ± 0.24
3.925SerIle: 3.925 ± 0.453
4.506SerLys: 4.506 ± 0.773
5.742SerLeu: 5.742 ± 0.667
1.308SerMet: 1.308 ± 0.303
3.925SerAsn: 3.925 ± 0.657
1.962SerPro: 1.962 ± 0.472
3.489SerGln: 3.489 ± 0.482
2.616SerArg: 2.616 ± 0.383
5.088SerSer: 5.088 ± 0.958
4.433SerThr: 4.433 ± 0.575
4.07SerVal: 4.07 ± 0.657
0.872SerTrp: 0.872 ± 0.261
2.253SerTyr: 2.253 ± 0.385
0.0SerXaa: 0.0 ± 0.0
Thr
6.614ThrAla: 6.614 ± 0.861
0.291ThrCys: 0.291 ± 0.151
5.524ThrAsp: 5.524 ± 0.646
3.707ThrGlu: 3.707 ± 0.488
2.835ThrPhe: 2.835 ± 0.519
4.506ThrGly: 4.506 ± 0.547
0.654ThrHis: 0.654 ± 0.295
4.87ThrIle: 4.87 ± 0.682
3.634ThrLys: 3.634 ± 0.413
5.451ThrLeu: 5.451 ± 0.612
1.308ThrMet: 1.308 ± 0.238
3.271ThrAsn: 3.271 ± 0.47
3.271ThrPro: 3.271 ± 0.507
2.18ThrGln: 2.18 ± 0.389
2.762ThrArg: 2.762 ± 0.485
4.579ThrSer: 4.579 ± 0.619
3.779ThrThr: 3.779 ± 0.471
4.724ThrVal: 4.724 ± 0.885
0.436ThrTrp: 0.436 ± 0.18
2.544ThrTyr: 2.544 ± 0.487
0.0ThrXaa: 0.0 ± 0.0
Val
5.306ValAla: 5.306 ± 0.625
0.291ValCys: 0.291 ± 0.135
5.378ValAsp: 5.378 ± 0.816
3.343ValGlu: 3.343 ± 0.653
1.454ValPhe: 1.454 ± 0.328
3.271ValGly: 3.271 ± 0.382
0.654ValHis: 0.654 ± 0.242
3.707ValIle: 3.707 ± 0.466
5.814ValLys: 5.814 ± 0.66
4.579ValLeu: 4.579 ± 0.664
1.89ValMet: 1.89 ± 0.353
3.997ValAsn: 3.997 ± 0.612
2.253ValPro: 2.253 ± 0.499
1.744ValGln: 1.744 ± 0.376
2.18ValArg: 2.18 ± 0.453
4.652ValSer: 4.652 ± 0.683
4.797ValThr: 4.797 ± 0.705
4.07ValVal: 4.07 ± 0.506
0.654ValTrp: 0.654 ± 0.183
1.817ValTyr: 1.817 ± 0.423
0.0ValXaa: 0.0 ± 0.0
Trp
0.799TrpAla: 0.799 ± 0.231
0.363TrpCys: 0.363 ± 0.177
0.945TrpAsp: 0.945 ± 0.291
0.727TrpGlu: 0.727 ± 0.257
0.436TrpPhe: 0.436 ± 0.168
0.654TrpGly: 0.654 ± 0.204
0.436TrpHis: 0.436 ± 0.193
0.872TrpIle: 0.872 ± 0.257
0.581TrpLys: 0.581 ± 0.25
1.599TrpLeu: 1.599 ± 0.471
0.073TrpMet: 0.073 ± 0.072
1.09TrpAsn: 1.09 ± 0.529
0.218TrpPro: 0.218 ± 0.119
0.945TrpGln: 0.945 ± 0.228
1.09TrpArg: 1.09 ± 0.377
0.727TrpSer: 0.727 ± 0.298
0.799TrpThr: 0.799 ± 0.254
1.018TrpVal: 1.018 ± 0.313
0.363TrpTrp: 0.363 ± 0.197
0.509TrpTyr: 0.509 ± 0.227
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.053TyrAla: 3.053 ± 0.701
0.436TyrCys: 0.436 ± 0.186
3.125TyrAsp: 3.125 ± 0.509
2.035TyrGlu: 2.035 ± 0.481
1.672TyrPhe: 1.672 ± 0.364
2.253TyrGly: 2.253 ± 0.371
0.654TyrHis: 0.654 ± 0.239
1.817TyrIle: 1.817 ± 0.445
2.18TyrLys: 2.18 ± 0.406
2.98TyrLeu: 2.98 ± 0.593
0.727TyrMet: 0.727 ± 0.28
1.672TyrAsn: 1.672 ± 0.315
1.817TyrPro: 1.817 ± 0.376
2.035TyrGln: 2.035 ± 0.374
2.18TyrArg: 2.18 ± 0.497
2.398TyrSer: 2.398 ± 0.589
2.398TyrThr: 2.398 ± 0.506
1.672TyrVal: 1.672 ± 0.339
0.436TyrTrp: 0.436 ± 0.2
1.381TyrTyr: 1.381 ± 0.36
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (13760 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski