Altererythrobacter sp. B11
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3568 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5Z034|A0A2Z5Z034_9SPHN OmpR/PhoB-type domain-containing protein OS=Altererythrobacter sp. B11 OX=2060312 GN=AEB_P2940 PE=4 SV=1
MM1 pKa = 7.19 EE2 pKa = 5.68 AEE4 pKa = 4.53 GSAGTSLLEE13 pKa = 4.27 LGQAAGMPLEE23 pKa = 4.71 GTCEE27 pKa = 3.9 GQMACSTCHH36 pKa = 6.34 VIVAQEE42 pKa = 4.04 WFAQLPPASEE52 pKa = 4.78 DD53 pKa = 3.66 EE54 pKa = 4.21 EE55 pKa = 6.14 DD56 pKa = 4.21 MLDD59 pKa = 4.11 LAPGVCATSRR69 pKa = 11.84 LSCQIDD75 pKa = 3.88 LVDD78 pKa = 4.42 EE79 pKa = 4.63 LDD81 pKa = 3.85 GLTVRR86 pKa = 11.84 IPPTSHH92 pKa = 7.26 DD93 pKa = 3.69 MQRR96 pKa = 11.84 GG97 pKa = 3.38
Molecular weight: 10.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.605
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.63
Grimsley 3.528
Solomon 3.745
Lehninger 3.694
Nozaki 3.897
DTASelect 4.037
Thurlkill 3.656
EMBOSS 3.681
Sillero 3.91
Patrickios 0.769
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A2Z5YRY2|A0A2Z5YRY2_9SPHN Uncharacterized protein OS=Altererythrobacter sp. B11 OX=2060312 GN=AEB_P0051 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.24 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.93 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.0 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 NKK41 pKa = 10.45 LSAA44 pKa = 3.94
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3568
0
3568
1161600
39
4941
325.6
35.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.3 ± 0.059
0.829 ± 0.016
5.811 ± 0.026
5.997 ± 0.043
3.585 ± 0.025
9.008 ± 0.054
1.96 ± 0.021
4.701 ± 0.026
2.676 ± 0.031
10.141 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.387 ± 0.023
2.433 ± 0.031
5.454 ± 0.033
3.238 ± 0.022
7.456 ± 0.056
5.278 ± 0.036
5.058 ± 0.056
7.008 ± 0.039
1.456 ± 0.019
2.224 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here