Galactobacter caseinivorans
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2599 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A496PHT0|A0A496PHT0_9MICC YhgE/Pip domain-containing protein OS=Galactobacter caseinivorans OX=2676123 GN=DWQ67_08730 PE=4 SV=1
MM1 pKa = 7.04 TADD4 pKa = 3.12 VHH6 pKa = 5.88 QAQEE10 pKa = 4.39 FYY12 pKa = 10.2 GTLLGWEE19 pKa = 4.23 FQGGDD24 pKa = 3.27 EE25 pKa = 4.38 EE26 pKa = 5.81 KK27 pKa = 10.96 YY28 pKa = 10.57 GGYY31 pKa = 11.04 LMAHH35 pKa = 6.69 KK36 pKa = 10.55 NGLPVAGMMQKK47 pKa = 9.91 QPDD50 pKa = 4.18 MADD53 pKa = 3.57 MPDD56 pKa = 2.78 VWTTYY61 pKa = 10.59 LGSTSADD68 pKa = 3.19 ASSAGALAAGGSVFFPPMDD87 pKa = 3.74 VPDD90 pKa = 3.75 QGIMGLVGDD99 pKa = 4.25 TTGAAIGIWQPQPMSGFEE117 pKa = 4.0 LTYY120 pKa = 10.84 EE121 pKa = 4.15 PGAPYY126 pKa = 9.87 WFEE129 pKa = 4.2 LFTRR133 pKa = 11.84 DD134 pKa = 3.95 FAGAQAFYY142 pKa = 11.02 RR143 pKa = 11.84 EE144 pKa = 4.24 AFDD147 pKa = 3.21 WDD149 pKa = 3.42 ITVVSDD155 pKa = 3.75 SDD157 pKa = 3.43 EE158 pKa = 3.91 FRR160 pKa = 11.84 YY161 pKa = 8.69 ATLGKK166 pKa = 10.25 DD167 pKa = 3.04 ADD169 pKa = 3.94 SRR171 pKa = 11.84 AGIMDD176 pKa = 4.25 ASAFLPQGVPDD187 pKa = 3.66 HH188 pKa = 6.08 WRR190 pKa = 11.84 VYY192 pKa = 10.72 LGVEE196 pKa = 4.25 DD197 pKa = 4.52 AVAAAKK203 pKa = 10.04 QVVALGGAILSGPEE217 pKa = 3.7 DD218 pKa = 3.76 TPYY221 pKa = 10.2 GTVVSIADD229 pKa = 3.47 PTGAMVLLASGYY241 pKa = 9.37 PGNGG245 pKa = 3.05
Molecular weight: 25.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.872
IPC_protein 3.872
Toseland 3.656
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.567
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.24
Thurlkill 3.706
EMBOSS 3.821
Sillero 3.999
Patrickios 1.914
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.89
Protein with the highest isoelectric point:
>tr|A0A496PLD2|A0A496PLD2_9MICC Uncharacterized protein OS=Galactobacter caseinivorans OX=2676123 GN=DWQ67_00170 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2599
0
2599
856916
24
1971
329.7
35.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.519 ± 0.062
0.531 ± 0.01
5.508 ± 0.041
5.743 ± 0.051
2.92 ± 0.029
9.18 ± 0.041
2.078 ± 0.022
3.756 ± 0.04
2.643 ± 0.05
10.334 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.979 ± 0.02
2.098 ± 0.026
5.58 ± 0.042
3.522 ± 0.031
6.892 ± 0.055
5.967 ± 0.034
5.834 ± 0.034
8.601 ± 0.048
1.519 ± 0.02
1.795 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here