Pantoea phage vB_PagP-SK1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Elunavirus; unclassified Elunavirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8NK79|A0A5P8NK79_9CAUD Major capsid protein OS=Pantoea phage vB_PagP-SK1 OX=2653646 PE=3 SV=1
MM1 pKa = 7.38GLSAFKK7 pKa = 10.89GIDD10 pKa = 2.92IMNDD14 pKa = 2.69INIWCAAFWILAAFIAGLAIGGVVCSDD41 pKa = 3.43TDD43 pKa = 3.71LDD45 pKa = 3.86

Molecular weight: 4.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8NK59|A0A5P8NK59_9CAUD Endonuclease OS=Pantoea phage vB_PagP-SK1 OX=2653646 PE=4 SV=1
MM1 pKa = 7.43LQRR4 pKa = 11.84TRR6 pKa = 11.84WKK8 pKa = 10.08HH9 pKa = 4.2VKK11 pKa = 9.07QALYY15 pKa = 9.98MMAYY19 pKa = 9.52GASKK23 pKa = 10.5RR24 pKa = 11.84KK25 pKa = 9.51AKK27 pKa = 10.17RR28 pKa = 11.84VLKK31 pKa = 10.37RR32 pKa = 11.84GRR34 pKa = 11.84KK35 pKa = 5.71VTARR39 pKa = 11.84QAASAVKK46 pKa = 8.98WAEE49 pKa = 3.86FTLASPMKK57 pKa = 10.03IIKK60 pKa = 9.85

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

11535

34

1332

274.6

30.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.256 ± 0.462

0.806 ± 0.151

6.589 ± 0.296

6.589 ± 0.298

3.762 ± 0.2

8.071 ± 0.322

1.743 ± 0.167

4.889 ± 0.207

6.32 ± 0.341

7.776 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.861 ± 0.216

4.283 ± 0.208

3.676 ± 0.144

4.482 ± 0.344

5.375 ± 0.171

5.713 ± 0.256

5.566 ± 0.249

6.649 ± 0.276

1.439 ± 0.191

3.156 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski