Mycobacterium virus Bethlehem

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5J5J0|Q5J5J0_9CAUD Uncharacterized protein OS=Mycobacterium virus Bethlehem OX=260121 GN=PBI_BETHLEHEM_34 PE=4 SV=1
MM1 pKa = 7.09SLKK4 pKa = 10.88VGDD7 pKa = 4.32LDD9 pKa = 3.97VVGVFVGDD17 pKa = 3.97AAAKK21 pKa = 9.88VYY23 pKa = 10.31VGAMKK28 pKa = 10.01IWPPIPDD35 pKa = 4.08FTPFSISSEE44 pKa = 4.16DD45 pKa = 3.28PGYY48 pKa = 9.65TDD50 pKa = 5.06IYY52 pKa = 10.72DD53 pKa = 3.73EE54 pKa = 4.75PVPEE58 pKa = 4.56GASGCWVTLVGGGGGGSGGGINGRR82 pKa = 11.84PGGNGGDD89 pKa = 3.68GYY91 pKa = 11.6VLIEE95 pKa = 4.07WEE97 pKa = 4.02

Molecular weight: 9.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5J5M3|Q5J5M3_9CAUD Uncharacterized protein OS=Mycobacterium virus Bethlehem OX=260121 GN=PBI_BETHLEHEM_1 PE=4 SV=1
MM1 pKa = 7.25SWEE4 pKa = 3.92SSDD7 pKa = 3.68RR8 pKa = 11.84RR9 pKa = 11.84EE10 pKa = 4.33RR11 pKa = 11.84LPADD15 pKa = 3.28WPRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.77VLRR25 pKa = 11.84AAGHH29 pKa = 6.4RR30 pKa = 11.84CQIRR34 pKa = 11.84YY35 pKa = 9.91ADD37 pKa = 3.88ICTGMATEE45 pKa = 4.27VDD47 pKa = 3.34HH48 pKa = 7.08VRR50 pKa = 11.84YY51 pKa = 9.53RR52 pKa = 11.84DD53 pKa = 3.64EE54 pKa = 4.46EE55 pKa = 4.54SPLQASCRR63 pKa = 11.84PCHH66 pKa = 5.76ARR68 pKa = 11.84KK69 pKa = 9.57SAMEE73 pKa = 4.09GVAQRR78 pKa = 11.84AKK80 pKa = 10.29LRR82 pKa = 11.84AMKK85 pKa = 9.94KK86 pKa = 9.63RR87 pKa = 11.84PPPRR91 pKa = 11.84HH92 pKa = 5.87PGRR95 pKa = 11.84RR96 pKa = 11.84SNN98 pKa = 3.52

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

16075

31

903

184.8

20.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.785 ± 0.474

0.709 ± 0.116

6.737 ± 0.217

6.849 ± 0.301

3.123 ± 0.128

8.267 ± 0.438

1.966 ± 0.183

5.014 ± 0.271

4.249 ± 0.262

8.628 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.114

3.204 ± 0.154

5.493 ± 0.232

3.278 ± 0.166

6.252 ± 0.397

5.86 ± 0.26

6.463 ± 0.261

7.266 ± 0.223

2.003 ± 0.116

2.862 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski