Amino acid dipepetide frequency for Mycobacterium virus Bethlehem

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.189AlaAla: 13.189 ± 1.661
0.622AlaCys: 0.622 ± 0.194
6.283AlaAsp: 6.283 ± 0.679
6.781AlaGlu: 6.781 ± 0.766
3.235AlaPhe: 3.235 ± 0.538
6.781AlaGly: 6.781 ± 0.657
1.431AlaHis: 1.431 ± 0.333
4.417AlaIle: 4.417 ± 0.608
4.293AlaLys: 4.293 ± 0.551
8.772AlaLeu: 8.772 ± 0.763
2.302AlaMet: 2.302 ± 0.396
2.986AlaAsn: 2.986 ± 0.401
4.479AlaPro: 4.479 ± 0.806
2.862AlaGln: 2.862 ± 0.457
6.097AlaArg: 6.097 ± 0.611
4.666AlaSer: 4.666 ± 0.612
5.972AlaThr: 5.972 ± 0.668
8.212AlaVal: 8.212 ± 0.717
1.68AlaTrp: 1.68 ± 0.294
2.8AlaTyr: 2.8 ± 0.382
0.0AlaXaa: 0.0 ± 0.0
Cys
0.56CysAla: 0.56 ± 0.209
0.0CysCys: 0.0 ± 0.0
0.435CysAsp: 0.435 ± 0.182
0.809CysGlu: 0.809 ± 0.204
0.124CysPhe: 0.124 ± 0.078
0.809CysGly: 0.809 ± 0.254
0.124CysHis: 0.124 ± 0.089
0.311CysIle: 0.311 ± 0.184
0.373CysLys: 0.373 ± 0.16
0.435CysLeu: 0.435 ± 0.172
0.249CysMet: 0.249 ± 0.134
0.249CysAsn: 0.249 ± 0.129
0.311CysPro: 0.311 ± 0.142
0.187CysGln: 0.187 ± 0.099
0.498CysArg: 0.498 ± 0.166
0.435CysSer: 0.435 ± 0.15
0.56CysThr: 0.56 ± 0.178
0.311CysVal: 0.311 ± 0.139
0.249CysTrp: 0.249 ± 0.117
0.062CysTyr: 0.062 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
5.972AspAla: 5.972 ± 0.585
0.684AspCys: 0.684 ± 0.193
4.915AspAsp: 4.915 ± 0.535
3.733AspGlu: 3.733 ± 0.5
2.426AspPhe: 2.426 ± 0.348
6.595AspGly: 6.595 ± 0.609
1.182AspHis: 1.182 ± 0.27
3.235AspIle: 3.235 ± 0.465
2.551AspLys: 2.551 ± 0.425
7.217AspLeu: 7.217 ± 0.751
1.244AspMet: 1.244 ± 0.241
1.742AspAsn: 1.742 ± 0.308
4.977AspPro: 4.977 ± 0.581
1.68AspGln: 1.68 ± 0.429
3.857AspArg: 3.857 ± 0.409
3.546AspSer: 3.546 ± 0.465
3.919AspThr: 3.919 ± 0.434
3.919AspVal: 3.919 ± 0.532
1.618AspTrp: 1.618 ± 0.323
2.364AspTyr: 2.364 ± 0.342
0.0AspXaa: 0.0 ± 0.0
Glu
6.035GluAla: 6.035 ± 0.809
0.498GluCys: 0.498 ± 0.215
4.977GluAsp: 4.977 ± 0.615
5.475GluGlu: 5.475 ± 0.713
2.302GluPhe: 2.302 ± 0.322
4.168GluGly: 4.168 ± 0.432
1.555GluHis: 1.555 ± 0.388
3.671GluIle: 3.671 ± 0.554
2.862GluLys: 2.862 ± 0.426
7.279GluLeu: 7.279 ± 0.552
1.431GluMet: 1.431 ± 0.27
1.68GluAsn: 1.68 ± 0.355
2.675GluPro: 2.675 ± 0.461
2.551GluGln: 2.551 ± 0.418
3.919GluArg: 3.919 ± 0.481
3.671GluSer: 3.671 ± 0.504
4.106GluThr: 4.106 ± 0.566
5.537GluVal: 5.537 ± 0.535
1.68GluTrp: 1.68 ± 0.371
2.364GluTyr: 2.364 ± 0.456
0.0GluXaa: 0.0 ± 0.0
Phe
2.24PheAla: 2.24 ± 0.293
0.435PheCys: 0.435 ± 0.174
2.986PheAsp: 2.986 ± 0.364
2.053PheGlu: 2.053 ± 0.346
0.56PhePhe: 0.56 ± 0.18
3.173PheGly: 3.173 ± 0.466
0.684PheHis: 0.684 ± 0.274
1.306PheIle: 1.306 ± 0.227
1.12PheLys: 1.12 ± 0.281
2.613PheLeu: 2.613 ± 0.407
0.56PheMet: 0.56 ± 0.221
1.058PheAsn: 1.058 ± 0.251
1.431PhePro: 1.431 ± 0.271
1.182PheGln: 1.182 ± 0.263
1.991PheArg: 1.991 ± 0.396
2.115PheSer: 2.115 ± 0.458
1.991PheThr: 1.991 ± 0.322
1.991PheVal: 1.991 ± 0.322
0.56PheTrp: 0.56 ± 0.169
1.182PheTyr: 1.182 ± 0.289
0.0PheXaa: 0.0 ± 0.0
Gly
6.47GlyAla: 6.47 ± 0.989
0.684GlyCys: 0.684 ± 0.248
6.159GlyAsp: 6.159 ± 0.569
5.039GlyGlu: 5.039 ± 0.538
2.8GlyPhe: 2.8 ± 0.435
7.528GlyGly: 7.528 ± 1.354
1.929GlyHis: 1.929 ± 0.371
4.479GlyIle: 4.479 ± 0.796
3.671GlyLys: 3.671 ± 0.552
7.963GlyLeu: 7.963 ± 0.999
1.618GlyMet: 1.618 ± 0.31
3.297GlyAsn: 3.297 ± 0.45
3.484GlyPro: 3.484 ± 0.553
2.302GlyGln: 2.302 ± 0.367
4.977GlyArg: 4.977 ± 0.548
5.599GlySer: 5.599 ± 0.521
4.604GlyThr: 4.604 ± 0.553
5.039GlyVal: 5.039 ± 0.531
2.737GlyTrp: 2.737 ± 0.49
2.302GlyTyr: 2.302 ± 0.348
0.0GlyXaa: 0.0 ± 0.0
His
1.804HisAla: 1.804 ± 0.364
0.062HisCys: 0.062 ± 0.058
1.244HisAsp: 1.244 ± 0.241
1.306HisGlu: 1.306 ± 0.281
0.684HisPhe: 0.684 ± 0.197
1.493HisGly: 1.493 ± 0.346
0.871HisHis: 0.871 ± 0.224
0.995HisIle: 0.995 ± 0.246
1.058HisLys: 1.058 ± 0.285
1.369HisLeu: 1.369 ± 0.362
0.124HisMet: 0.124 ± 0.092
0.311HisAsn: 0.311 ± 0.124
1.493HisPro: 1.493 ± 0.325
0.995HisGln: 0.995 ± 0.254
1.555HisArg: 1.555 ± 0.341
0.56HisSer: 0.56 ± 0.175
0.995HisThr: 0.995 ± 0.272
1.68HisVal: 1.68 ± 0.316
0.498HisTrp: 0.498 ± 0.149
0.56HisTyr: 0.56 ± 0.206
0.0HisXaa: 0.0 ± 0.0
Ile
6.283IleAla: 6.283 ± 0.736
0.249IleCys: 0.249 ± 0.117
3.359IleAsp: 3.359 ± 0.416
3.733IleGlu: 3.733 ± 0.533
1.058IlePhe: 1.058 ± 0.249
3.857IleGly: 3.857 ± 0.453
0.933IleHis: 0.933 ± 0.218
1.493IleIle: 1.493 ± 0.29
1.866IleLys: 1.866 ± 0.342
3.422IleLeu: 3.422 ± 0.361
0.871IleMet: 0.871 ± 0.193
2.053IleAsn: 2.053 ± 0.344
3.173IlePro: 3.173 ± 0.392
1.493IleGln: 1.493 ± 0.401
3.795IleArg: 3.795 ± 0.441
3.111IleSer: 3.111 ± 0.415
3.484IleThr: 3.484 ± 0.446
3.297IleVal: 3.297 ± 0.577
0.809IleTrp: 0.809 ± 0.195
1.804IleTyr: 1.804 ± 0.259
0.0IleXaa: 0.0 ± 0.0
Lys
3.857LysAla: 3.857 ± 0.531
0.311LysCys: 0.311 ± 0.14
2.613LysAsp: 2.613 ± 0.419
2.053LysGlu: 2.053 ± 0.437
1.493LysPhe: 1.493 ± 0.298
2.675LysGly: 2.675 ± 0.366
1.12LysHis: 1.12 ± 0.257
2.426LysIle: 2.426 ± 0.423
1.929LysLys: 1.929 ± 0.428
3.733LysLeu: 3.733 ± 0.462
0.995LysMet: 0.995 ± 0.219
1.804LysAsn: 1.804 ± 0.311
2.302LysPro: 2.302 ± 0.461
1.618LysGln: 1.618 ± 0.319
3.297LysArg: 3.297 ± 0.483
2.613LysSer: 2.613 ± 0.386
2.364LysThr: 2.364 ± 0.445
3.733LysVal: 3.733 ± 0.516
0.809LysTrp: 0.809 ± 0.212
0.747LysTyr: 0.747 ± 0.231
0.0LysXaa: 0.0 ± 0.0
Leu
9.456LeuAla: 9.456 ± 0.83
0.311LeuCys: 0.311 ± 0.133
6.035LeuAsp: 6.035 ± 0.515
5.599LeuGlu: 5.599 ± 0.606
2.115LeuPhe: 2.115 ± 0.388
7.403LeuGly: 7.403 ± 0.842
1.618LeuHis: 1.618 ± 0.319
4.915LeuIle: 4.915 ± 0.544
4.417LeuLys: 4.417 ± 0.525
5.537LeuLeu: 5.537 ± 0.544
1.555LeuMet: 1.555 ± 0.271
2.8LeuAsn: 2.8 ± 0.378
5.91LeuPro: 5.91 ± 0.593
2.426LeuGln: 2.426 ± 0.449
6.283LeuArg: 6.283 ± 0.582
5.786LeuSer: 5.786 ± 0.532
6.221LeuThr: 6.221 ± 0.5
4.541LeuVal: 4.541 ± 0.518
1.058LeuTrp: 1.058 ± 0.277
2.302LeuTyr: 2.302 ± 0.395
0.0LeuXaa: 0.0 ± 0.0
Met
2.302MetAla: 2.302 ± 0.305
0.0MetCys: 0.0 ± 0.0
1.12MetAsp: 1.12 ± 0.259
1.555MetGlu: 1.555 ± 0.34
0.498MetPhe: 0.498 ± 0.162
1.244MetGly: 1.244 ± 0.271
0.249MetHis: 0.249 ± 0.12
0.684MetIle: 0.684 ± 0.212
1.12MetLys: 1.12 ± 0.221
1.12MetLeu: 1.12 ± 0.229
0.124MetMet: 0.124 ± 0.095
1.058MetAsn: 1.058 ± 0.202
1.058MetPro: 1.058 ± 0.251
0.747MetGln: 0.747 ± 0.175
1.12MetArg: 1.12 ± 0.226
2.177MetSer: 2.177 ± 0.381
2.115MetThr: 2.115 ± 0.307
0.871MetVal: 0.871 ± 0.243
0.311MetTrp: 0.311 ± 0.126
0.435MetTyr: 0.435 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
3.297AsnAla: 3.297 ± 0.464
0.0AsnCys: 0.0 ± 0.0
1.991AsnAsp: 1.991 ± 0.394
1.804AsnGlu: 1.804 ± 0.33
0.871AsnPhe: 0.871 ± 0.234
3.671AsnGly: 3.671 ± 0.528
0.684AsnHis: 0.684 ± 0.201
1.742AsnIle: 1.742 ± 0.342
0.747AsnLys: 0.747 ± 0.239
2.426AsnLeu: 2.426 ± 0.361
0.622AsnMet: 0.622 ± 0.17
0.933AsnAsn: 0.933 ± 0.227
2.862AsnPro: 2.862 ± 0.333
1.182AsnGln: 1.182 ± 0.284
1.431AsnArg: 1.431 ± 0.375
1.866AsnSer: 1.866 ± 0.429
1.929AsnThr: 1.929 ± 0.309
2.115AsnVal: 2.115 ± 0.389
0.747AsnTrp: 0.747 ± 0.189
1.12AsnTyr: 1.12 ± 0.27
0.0AsnXaa: 0.0 ± 0.0
Pro
5.226ProAla: 5.226 ± 0.578
0.56ProCys: 0.56 ± 0.198
4.604ProAsp: 4.604 ± 0.586
4.23ProGlu: 4.23 ± 0.51
2.053ProPhe: 2.053 ± 0.376
5.039ProGly: 5.039 ± 0.603
0.747ProHis: 0.747 ± 0.212
2.488ProIle: 2.488 ± 0.375
2.053ProLys: 2.053 ± 0.313
4.541ProLeu: 4.541 ± 0.536
0.933ProMet: 0.933 ± 0.24
1.431ProAsn: 1.431 ± 0.277
2.737ProPro: 2.737 ± 0.459
1.555ProGln: 1.555 ± 0.342
2.302ProArg: 2.302 ± 0.402
3.857ProSer: 3.857 ± 0.461
4.044ProThr: 4.044 ± 0.464
4.293ProVal: 4.293 ± 0.563
0.809ProTrp: 0.809 ± 0.282
1.431ProTyr: 1.431 ± 0.307
0.0ProXaa: 0.0 ± 0.0
Gln
3.235GlnAla: 3.235 ± 0.522
0.124GlnCys: 0.124 ± 0.089
1.12GlnAsp: 1.12 ± 0.33
2.053GlnGlu: 2.053 ± 0.32
1.182GlnPhe: 1.182 ± 0.266
2.675GlnGly: 2.675 ± 0.334
0.56GlnHis: 0.56 ± 0.154
2.8GlnIle: 2.8 ± 0.511
1.058GlnLys: 1.058 ± 0.237
3.546GlnLeu: 3.546 ± 0.491
0.871GlnMet: 0.871 ± 0.254
0.56GlnAsn: 0.56 ± 0.177
2.053GlnPro: 2.053 ± 0.319
1.929GlnGln: 1.929 ± 0.381
1.866GlnArg: 1.866 ± 0.407
1.742GlnSer: 1.742 ± 0.283
1.68GlnThr: 1.68 ± 0.33
2.675GlnVal: 2.675 ± 0.372
0.498GlnTrp: 0.498 ± 0.15
0.56GlnTyr: 0.56 ± 0.17
0.0GlnXaa: 0.0 ± 0.0
Arg
5.91ArgAla: 5.91 ± 0.624
0.747ArgCys: 0.747 ± 0.196
3.173ArgAsp: 3.173 ± 0.435
4.915ArgGlu: 4.915 ± 0.632
2.115ArgPhe: 2.115 ± 0.431
4.106ArgGly: 4.106 ± 0.511
1.12ArgHis: 1.12 ± 0.252
3.484ArgIle: 3.484 ± 0.483
3.484ArgLys: 3.484 ± 0.547
5.91ArgLeu: 5.91 ± 0.72
1.742ArgMet: 1.742 ± 0.327
2.115ArgAsn: 2.115 ± 0.409
2.364ArgPro: 2.364 ± 0.361
2.053ArgGln: 2.053 ± 0.36
4.853ArgArg: 4.853 ± 0.572
3.733ArgSer: 3.733 ± 0.46
2.862ArgThr: 2.862 ± 0.412
4.915ArgVal: 4.915 ± 0.518
1.306ArgTrp: 1.306 ± 0.314
1.618ArgTyr: 1.618 ± 0.291
0.0ArgXaa: 0.0 ± 0.0
Ser
5.786SerAla: 5.786 ± 0.679
0.498SerCys: 0.498 ± 0.191
3.546SerAsp: 3.546 ± 0.493
3.982SerGlu: 3.982 ± 0.518
1.866SerPhe: 1.866 ± 0.369
5.724SerGly: 5.724 ± 0.689
1.431SerHis: 1.431 ± 0.332
2.986SerIle: 2.986 ± 0.484
2.675SerLys: 2.675 ± 0.445
5.35SerLeu: 5.35 ± 0.594
1.555SerMet: 1.555 ± 0.329
2.177SerAsn: 2.177 ± 0.338
3.048SerPro: 3.048 ± 0.503
1.929SerGln: 1.929 ± 0.311
3.048SerArg: 3.048 ± 0.378
2.924SerSer: 2.924 ± 0.532
2.8SerThr: 2.8 ± 0.347
4.168SerVal: 4.168 ± 0.458
1.369SerTrp: 1.369 ± 0.3
1.742SerTyr: 1.742 ± 0.362
0.0SerXaa: 0.0 ± 0.0
Thr
6.035ThrAla: 6.035 ± 0.727
0.311ThrCys: 0.311 ± 0.155
4.23ThrAsp: 4.23 ± 0.492
4.79ThrGlu: 4.79 ± 0.505
2.426ThrPhe: 2.426 ± 0.387
6.221ThrGly: 6.221 ± 0.61
1.182ThrHis: 1.182 ± 0.325
2.8ThrIle: 2.8 ± 0.502
2.488ThrLys: 2.488 ± 0.338
5.786ThrLeu: 5.786 ± 0.581
0.933ThrMet: 0.933 ± 0.201
1.68ThrAsn: 1.68 ± 0.349
3.982ThrPro: 3.982 ± 0.486
1.742ThrGln: 1.742 ± 0.346
3.173ThrArg: 3.173 ± 0.506
3.235ThrSer: 3.235 ± 0.468
4.666ThrThr: 4.666 ± 0.606
5.91ThrVal: 5.91 ± 0.539
1.058ThrTrp: 1.058 ± 0.254
1.991ThrTyr: 1.991 ± 0.367
0.0ThrXaa: 0.0 ± 0.0
Val
6.346ValAla: 6.346 ± 0.65
0.435ValCys: 0.435 ± 0.18
5.661ValAsp: 5.661 ± 0.604
5.226ValGlu: 5.226 ± 0.477
1.991ValPhe: 1.991 ± 0.351
5.164ValGly: 5.164 ± 0.714
1.306ValHis: 1.306 ± 0.248
3.235ValIle: 3.235 ± 0.469
3.111ValLys: 3.111 ± 0.45
5.475ValLeu: 5.475 ± 0.624
1.12ValMet: 1.12 ± 0.28
2.302ValAsn: 2.302 ± 0.354
4.417ValPro: 4.417 ± 0.5
2.364ValGln: 2.364 ± 0.376
4.915ValArg: 4.915 ± 0.626
4.666ValSer: 4.666 ± 0.527
6.159ValThr: 6.159 ± 0.573
5.35ValVal: 5.35 ± 0.603
1.12ValTrp: 1.12 ± 0.284
2.302ValTyr: 2.302 ± 0.372
0.0ValXaa: 0.0 ± 0.0
Trp
1.555TrpAla: 1.555 ± 0.33
0.311TrpCys: 0.311 ± 0.143
1.431TrpAsp: 1.431 ± 0.268
0.995TrpGlu: 0.995 ± 0.237
0.809TrpPhe: 0.809 ± 0.225
1.804TrpGly: 1.804 ± 0.307
0.311TrpHis: 0.311 ± 0.148
1.058TrpIle: 1.058 ± 0.199
0.373TrpLys: 0.373 ± 0.27
1.68TrpLeu: 1.68 ± 0.32
0.311TrpMet: 0.311 ± 0.155
0.622TrpAsn: 0.622 ± 0.178
0.871TrpPro: 0.871 ± 0.255
0.995TrpGln: 0.995 ± 0.213
1.12TrpArg: 1.12 ± 0.311
0.809TrpSer: 0.809 ± 0.214
1.929TrpThr: 1.929 ± 0.31
2.177TrpVal: 2.177 ± 0.295
0.498TrpTrp: 0.498 ± 0.177
0.373TrpTyr: 0.373 ± 0.137
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.302TyrAla: 2.302 ± 0.348
0.249TyrCys: 0.249 ± 0.153
1.493TyrAsp: 1.493 ± 0.332
2.364TyrGlu: 2.364 ± 0.361
0.622TyrPhe: 0.622 ± 0.169
2.551TyrGly: 2.551 ± 0.419
0.622TyrHis: 0.622 ± 0.221
1.618TyrIle: 1.618 ± 0.375
1.244TyrLys: 1.244 ± 0.231
2.115TyrLeu: 2.115 ± 0.331
0.684TyrMet: 0.684 ± 0.167
1.182TyrAsn: 1.182 ± 0.331
1.306TyrPro: 1.306 ± 0.272
0.995TyrGln: 0.995 ± 0.232
2.426TyrArg: 2.426 ± 0.453
1.493TyrSer: 1.493 ± 0.284
2.24TyrThr: 2.24 ± 0.379
1.929TyrVal: 1.929 ± 0.383
0.622TyrTrp: 0.622 ± 0.213
0.56TyrTyr: 0.56 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (16075 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski