Circovirus-like genome DCCV-9
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A190WHL0|A0A190WHL0_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-9 OX=1788449 PE=4 SV=1
MM1 pKa = 7.47 MFRR4 pKa = 11.84 ILSGLMATADD14 pKa = 3.59 KK15 pKa = 10.52 KK16 pKa = 10.64 RR17 pKa = 11.84 CFSIMSRR24 pKa = 11.84 TATVLSMLIFFVRR37 pKa = 11.84 SIVILFKK44 pKa = 11.12 SPSKK48 pKa = 10.46 EE49 pKa = 3.85 DD50 pKa = 3.41 SSLGTQSGSTLPPLDD65 pKa = 4.23 SLPDD69 pKa = 3.66 AGLILEE75 pKa = 4.28 TLARR79 pKa = 11.84 STEE82 pKa = 3.94 EE83 pKa = 4.6 LIAILDD89 pKa = 3.87 QEE91 pKa = 4.86 STCSSITHH99 pKa = 6.15 TT100 pKa = 4.41
Molecular weight: 10.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.563
IPC2_protein 5.69
IPC_protein 5.537
Toseland 5.626
ProMoST 5.728
Dawson 5.639
Bjellqvist 5.69
Wikipedia 5.575
Rodwell 5.588
Grimsley 5.614
Solomon 5.639
Lehninger 5.614
Nozaki 5.817
DTASelect 5.995
Thurlkill 5.83
EMBOSS 5.792
Sillero 5.906
Patrickios 4.622
IPC_peptide 5.652
IPC2_peptide 5.893
IPC2.peptide.svr19 6.148
Protein with the highest isoelectric point:
>tr|A0A190WHL0|A0A190WHL0_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-9 OX=1788449 PE=4 SV=1
MM1 pKa = 6.1 VTKK4 pKa = 10.37 SAYY7 pKa = 8.71 IKK9 pKa = 10.51 KK10 pKa = 10.25 GSRR13 pKa = 11.84 LAKK16 pKa = 10.25 ARR18 pKa = 11.84 ARR20 pKa = 11.84 PYY22 pKa = 10.54 KK23 pKa = 9.98 KK24 pKa = 10.43 SKK26 pKa = 10.15 ASKK29 pKa = 8.99 TFAKK33 pKa = 10.01 KK34 pKa = 10.11 VKK36 pKa = 9.38 AVVMKK41 pKa = 10.02 TVEE44 pKa = 4.41 TKK46 pKa = 10.36 SRR48 pKa = 11.84 PWGYY52 pKa = 11.36 GKK54 pKa = 10.87 LEE56 pKa = 4.4 LNHH59 pKa = 6.1 NVPYY63 pKa = 10.71 AITLNGTGNPGPMPPQGTQDD83 pKa = 3.41 YY84 pKa = 10.12 EE85 pKa = 4.7 RR86 pKa = 11.84 IGDD89 pKa = 4.26 IINTSGIKK97 pKa = 9.97 LRR99 pKa = 11.84 LMCGQKK105 pKa = 10.3 ADD107 pKa = 4.08 RR108 pKa = 11.84 PNVTFKK114 pKa = 10.48 FWVVQVPKK122 pKa = 10.82 GSGYY126 pKa = 10.89 SYY128 pKa = 10.77 NQWFMNYY135 pKa = 8.56 TGNCLLDD142 pKa = 4.13 SINTDD147 pKa = 3.72 FVKK150 pKa = 10.61 ILATHH155 pKa = 5.77 TWKK158 pKa = 10.98 APEE161 pKa = 4.31 TYY163 pKa = 10.02 SAEE166 pKa = 4.03 TRR168 pKa = 11.84 EE169 pKa = 4.2 VTFTKK174 pKa = 10.14 QWWIPRR180 pKa = 11.84 RR181 pKa = 11.84 RR182 pKa = 11.84 VVKK185 pKa = 10.32 FGPTNASLSHH195 pKa = 6.61 NDD197 pKa = 3.11 SDD199 pKa = 4.8 VHH201 pKa = 6.28 LLCAAYY207 pKa = 9.75 DD208 pKa = 3.64 AYY210 pKa = 10.07 GTLVTDD216 pKa = 4.42 NIAYY220 pKa = 9.15 VQLHH224 pKa = 5.82 VEE226 pKa = 4.29 TFYY229 pKa = 11.24 RR230 pKa = 11.84 DD231 pKa = 3.33 PP232 pKa = 4.9
Molecular weight: 26.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.144
IPC2_protein 9.311
IPC_protein 9.224
Toseland 9.867
ProMoST 9.589
Dawson 10.101
Bjellqvist 9.794
Wikipedia 10.277
Rodwell 10.54
Grimsley 10.189
Solomon 10.131
Lehninger 10.087
Nozaki 9.897
DTASelect 9.78
Thurlkill 9.94
EMBOSS 10.277
Sillero 10.028
Patrickios 9.663
IPC_peptide 10.116
IPC2_peptide 8.419
IPC2.peptide.svr19 8.246
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
661
100
329
220.3
25.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.295 ± 1.147
2.118 ± 0.438
6.354 ± 1.274
4.539 ± 0.729
3.782 ± 0.327
6.203 ± 0.61
1.815 ± 0.229
4.539 ± 1.283
7.11 ± 1.163
8.018 ± 1.623
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.874 ± 0.578
3.328 ± 1.155
4.992 ± 0.324
3.48 ± 0.584
6.505 ± 0.965
7.11 ± 2.415
7.413 ± 1.363
6.959 ± 1.067
2.572 ± 0.763
4.992 ± 1.38
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here